绞股蓝皂苷类成分的超高效液相色谱指纹图谱研究
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摘要
目的建立绞股蓝药材皂苷类化合物的超高效液相色谱(UPLC)指纹图谱法,为绞股蓝药材的质量控制和质量评价提供参考。方法色谱柱采用ACQUITY UPLC BEH C18柱(100 mm×2. 1 mm,1. 7μm),流动相以乙腈(A)-水(B)梯度洗脱,检测波长为203 nm,流速为0. 2 mL/min,柱温为30℃。结果绞股蓝药材皂苷类化合物指纹图谱有9个共有峰,指认了1个S峰为参照峰; 11批绞股蓝药材的相似度达0. 88以上,方法学考察符合指纹图谱的技术要求。结论该方法较传统的高效液相色谱法有更好的分离效果,准确性、精密度、重复性高,可为绞股蓝药材的质量控制提供参考。
Objective To establish an UPLC fingerprint of saponins from Gynostemma pentaphyllum,and to provide a reference for quality control and quality evaluation of Gynostemma pentaphyllum. Methods The chromatographic column was ACQUITY UPLC BEH C18column( 100 mm × 2. 1 mm, 1. 7 μm), the mobile phase was acetonitrile( A)-water( B) with gradient elution, the detection wavelength was 203 nm,the flow rate was 0. 2 mL/min,and the column temperature was 30 ℃. Results There were 9 common peaks in the fingerprint of saponins from Gynostemma pentaphyllum,and one S peak was identified as the reference peak. The similarity of 11 batches of Gynostemma pentaphyllum reached above 0. 88, and the methodological investigation met the technical requirements of the fingerprint. Conclusion Compared with the traditional HPLC method,this method has better separation effect,accuracy,precision and repeatability,which can provide a reference for the quality control of Gynostemma pentaphyllum.
Objective To establish an UPLC fingerprint of saponins from Gynostemma pentaphyllum,and to provide a reference for quality control and quality evaluation of Gynostemma pentaphyllum. Methods The chromatographic column was ACQUITY UPLC BEH C18column( 100 mm × 2. 1 mm, 1. 7 μm), the mobile phase was acetonitrile( A)-water( B) with gradient elution, the detection wavelength was 203 nm,the flow rate was 0. 2 mL/min,and the column temperature was 30 ℃. Results There were 9 common peaks in the fingerprint of saponins from Gynostemma pentaphyllum,and one S peak was identified as the reference peak. The similarity of 11 batches of Gynostemma pentaphyllum reached above 0. 88, and the methodological investigation met the technical requirements of the fingerprint. Conclusion Compared with the traditional HPLC method,this method has better separation effect,accuracy,precision and repeatability,which can provide a reference for the quality control of Gynostemma pentaphyllum.
引文
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[7]辛,李山姗,王淼,等.绞股蓝总苷片的指纹图谱及含量测定[J].沈阳药科大学学报,2016,33(3):208-214.
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[10]刘芳,胡坪,ANNIKA K,等.绞股蓝药材黄酮类化合物的HPLC指纹图谱研究[J].中成药,2009,31(4):493-495.
[11]刘世彪,林如,胡正海.绞股蓝属5种植物的茎叶结构和总皂苷含量差异[J].福建农林大学学报(自然版),2006,35(5):495-499.
[12]王临润,黄明珠.中国东南部4种绞股蓝中黄酮成分的分析[J].中草药,2007,38(4):618-619.
[13]王,郭志刚,赵桂仿.利用ISSR分析6种绞股蓝属植物的遗传多样性与亲缘关系[J].西北大学学报:自然科学版,2008,38(5):767-770.
[14]赵瑜,王佩香,邹芳平,等.绞股蓝叶龄变化规律及其应用研究[J].中国农学通报,2005,21(5):230.
[2]浙江植物志委员会.浙江植物志(第六卷)[M].杭州:浙江科学技术出版社,1993:182-184.
[3]钟辉.绞股蓝黄酮指纹图谱研究[J].湖北中医药大学学报,2010,12(3):31-33.
[4]彭亮,李诒光,陈杰,等. HPLC测定不同产地、不同品种绞股蓝中芦丁和槲皮素的含量[J].中国实验方剂学杂志,2016,22(6):45-47.
[5]范冬冬,匡艳辉,向世勰,等.绞股蓝化学成分及其药理活性研究进展[J].中国药学杂志,2017,52(5):342-352
[6]蒋慧宣,陈粲文,徐茂保,等. HPLC法同时测定绞股蓝药材中8种皂苷的含量[J].广州中医药大学学报,2018,35(2):324-328.
[7]辛,李山姗,王淼,等.绞股蓝总苷片的指纹图谱及含量测定[J].沈阳药科大学学报,2016,33(3):208-214.
[8]李浩飞. RP-HPLC-ELSD同时测定绞股蓝中各成份含量[J].中国卫生产业,2015(15):5-8.
[9]陈丹,李柯,吴梅青,等.湖南省不同产地绞股蓝药材中总皂苷含量测定[J].中国民族民间医药,2013,22(7):17-18.
[10]刘芳,胡坪,ANNIKA K,等.绞股蓝药材黄酮类化合物的HPLC指纹图谱研究[J].中成药,2009,31(4):493-495.
[11]刘世彪,林如,胡正海.绞股蓝属5种植物的茎叶结构和总皂苷含量差异[J].福建农林大学学报(自然版),2006,35(5):495-499.
[12]王临润,黄明珠.中国东南部4种绞股蓝中黄酮成分的分析[J].中草药,2007,38(4):618-619.
[13]王,郭志刚,赵桂仿.利用ISSR分析6种绞股蓝属植物的遗传多样性与亲缘关系[J].西北大学学报:自然科学版,2008,38(5):767-770.
[14]赵瑜,王佩香,邹芳平,等.绞股蓝叶龄变化规律及其应用研究[J].中国农学通报,2005,21(5):230.