黄秋葵荚果不同生长时期蛋白质变化初探
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摘要
黄秋葵是重要的蔬菜,其鲜果营养丰富,但采收过晚荚果纤维木质化严重,影响黄秋葵的口感,试验通过Tris-酚法对黄秋葵荚果不同生长时期的全蛋白质进行提取,通过聚丙烯酰胺凝胶电泳将这些全蛋白进行分离,对得到的电泳图进行比较研究。结果表明:黄秋葵嫩果期的蛋白质含量远低于荚果老化期,其荚果最佳采收时间在生长第7天到第10天。
Okra is an important vegetable, and its fresh fruit is rich in nutrition. Missing the harvest time, the okra pod senescence will affect the taste. The whole protein is extracted by Tris-phenol method, and the whole protein is separated by polyacrylamide gel electrophoresis. The obtained electrophoretic maps were compared. The results showed that the protein content of okra during the tender fruit stage was much lower than that of the pods aging stage, and the best harvest time was seventh days to tenth days in growth.
Okra is an important vegetable, and its fresh fruit is rich in nutrition. Missing the harvest time, the okra pod senescence will affect the taste. The whole protein is extracted by Tris-phenol method, and the whole protein is separated by polyacrylamide gel electrophoresis. The obtained electrophoretic maps were compared. The results showed that the protein content of okra during the tender fruit stage was much lower than that of the pods aging stage, and the best harvest time was seventh days to tenth days in growth.
引文
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[16]李慧妍,秦文,辛松林,等.壳聚糖和1-甲基环丙烯对黄秋葵果实低温贮藏品质及生理的影响[J].食品与发酵工业,2015,(12):202-207.
[2]刘连军.黄秋葵的应用价值及栽培技术[J].现代农业科技,2015,(19):101-102.
[3]娄风菊,连立峰,金海潮,等.黄秋葵的栽培与应用[J].特种经济动植物,2004,(7):38.
[4]吕美云,郭孟萍.食用秋葵6种生命元素测定及药用价值初探[J].江西中医学院学报,2001,(2):72.
[5]王君耀,周峻,汤谷平.黄秋葵抗疲劳作用的研究[J].中国现代应用药学,2003,(4):316-317.
[6]杨毅,金祖汉,毛培江,等.黄秋葵提取物抗疲劳的实验研究[J].中国现代应用药学,2012,(12):1091-1094.
[7]Xia F B,Yu Z,Li M Q,et al.Antioxidant and Anti-Fatigue Constituents of Okra[J].Nutrients,2015,7(10):8846-8858.
[8]Liu I M,TZeng T F,Liou S S,et al.Improvement of insulin sensitivity in obese Zucker rats by myricetin extracted from Abelmoschus moschatus[J].Planta Medica,2007.73(10):1054-1060.
[9]Liu I M,TZeng T F,Liou S S,et al.Abelmoschus moschatus(Malvaceae),an aromatic plant,suitable for medical or food uses to improve insulin sensitivity[J].Phytotherapy Research,2009,24(2):233-239.
[10]Okada Y,Okada M,Sagesaka Y.Screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3T3-L1 adipocytes[J].Plant Foods Hum Nutr,2010,65(3):225-232.
[11]Sabitha V,Ramachandran S,Naveen K R,et al.Antidiabetic and antihyperlipidemic potential of Abelmoschus esculentus(L.)Moench.in streptozotocin-induced diabetic rats[J].Journal of Pharmacy&Bioallied Sciences,2011,3(3):397-402.
[12]Thole C,Brandt S,Ahmed N,et al.Acetylated rhamnogalacturonans from immature fruits of Abelmoschus esculentus inhibit the adhesion of helicobacter pylori to human gastric cells by interaction with outer membrane proteins[J].Molecules,2015,20(9):16770-16787.
[13]李学智.黄秋葵嫩荚的食用品质及贮藏的生理研究[D].杭州:浙江大学,2003.
[14]许如意,罗丰,袁廷庆,等.不同采摘期对黄秋葵果实性状和品质的影响[J].长江蔬菜,2011,(2):18-20.
[15]陈江萍.黄秋葵保鲜贮藏技术的研究[J].食品研究与开发,2010,(8):186-189.
[16]李慧妍,秦文,辛松林,等.壳聚糖和1-甲基环丙烯对黄秋葵果实低温贮藏品质及生理的影响[J].食品与发酵工业,2015,(12):202-207.