化胃舒复方配伍对红参苷类成分的影响
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摘要
目的:探讨化胃舒复方配伍对制剂中人参皂苷Rg1、Re和Rb1含量影响。方法:采用高效液相色谱法,建立红参中人参皂苷Rg1、Re和Rb1含量测定方法;应用均匀设计法对处方中药材进行配伍实验,对数据进行回归分析,考查化胃舒处方中其它药味对红参中苷类成分的影响。结果:配伍后玄参中的1种成分能使Rg1含量升高,黄芪、陈皮、和乌梅中3种成分使Rg1含量降低;有2个归属于黄芪和1个归属于乌梅的成分使Re含量升高,有1个归属于黄芪的成分使Re含量降低;有2个来自陈皮成分,1个来自乌梅成分,1个来自黄芪成分使Rb1含量升高,有1个归属于乌梅的成分使Rb1含量降低。结论:配伍后处方中的黄芪、乌梅、陈皮和玄参中的一些成分可能对化胃舒颗粒中人参皂苷类成分含量有显著影响。
Objective:To explore the effect of the compatibility of Huaweishu Granules on the contents of ginsenoside Rg1,Re and Rb1.Methods:We used the high performance liquid chromatography to establish the determination method of ginsenoside Rg1,Re and Rb1 in red ginseng and the method of homogeneous design to conduct compatibility experiment,regression analysis of data,to test the influence of other ingredients on glycosides in red ginseng in Huaweishu Granules.Results:The compatibility of a component in Radix Scrophulariae can make the content of Rg1 increase.The content of Rg1 was reduced by astragalus,tangerine and wumei.There were two components belonging to the astragalus and one belonging to wumei,which increases the Re content,and one of the components belonging to the astragalus reduces the Re content,there are two content ingredients from citrus peel,one constituents from fructus mume,one content from astragalus composition Rb1 content increased,one content belong to the plum composition to make Rb1 content reduced.Conclusion:After compatibility,some components in the prescription of radix astragali,fructus mume,citrus peel and radix scrophulariae may be have a significant impact on the content of ginsenosides in Huaweishu Granules.
Objective:To explore the effect of the compatibility of Huaweishu Granules on the contents of ginsenoside Rg1,Re and Rb1.Methods:We used the high performance liquid chromatography to establish the determination method of ginsenoside Rg1,Re and Rb1 in red ginseng and the method of homogeneous design to conduct compatibility experiment,regression analysis of data,to test the influence of other ingredients on glycosides in red ginseng in Huaweishu Granules.Results:The compatibility of a component in Radix Scrophulariae can make the content of Rg1 increase.The content of Rg1 was reduced by astragalus,tangerine and wumei.There were two components belonging to the astragalus and one belonging to wumei,which increases the Re content,and one of the components belonging to the astragalus reduces the Re content,there are two content ingredients from citrus peel,one constituents from fructus mume,one content from astragalus composition Rb1 content increased,one content belong to the plum composition to make Rb1 content reduced.Conclusion:After compatibility,some components in the prescription of radix astragali,fructus mume,citrus peel and radix scrophulariae may be have a significant impact on the content of ginsenosides in Huaweishu Granules.
引文
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[16] 王京辉,陈晶,郭洪祝,等.进口红参与国产红参皂苷类成分含量及鉴别成分研究[J].中国药学杂志,2018,53(13):1070-1076.
[2] 王光函,赵玥,邹桂欣.化胃舒颗粒的定性鉴别研究[J].时珍国医国药,2012,23(8):1978-1979.
[3] 王光函,邸子真,邹桂欣.化胃舒颗粒中黄芪甲苷和人参总皂苷的测定[J].时珍国医国药,2013,24(3):567-568.
[4] 岳美露,王光函,李国信,等.HPLC法测定化胃舒颗粒中哈巴俄苷的含量[J].海峡药学,2014,26(6):45-47.
[5] 吴怡,尤献民,邹桂欣,等.化胃舒颗粒剂的处方研究[J].中成药,2013,35(1):64-67.
[6] 殷紫,向绍杰,乔敏,等.化胃舒颗粒对抗化疗药引起呕吐药效学实验研究及作用机制探讨[J].辽宁中医杂志,2013,40(6):1252-1255.
[7] 孙娜,徐钢,徐珊.人参炮制对其化学成分和药理作用的影响[J].中国药房,2016,27(6):857-859.
[8] 王越,孙佳明,张辉.人参蒸炖炮制的研究[J].长春中医药大学学报,2015,31(6):1130-1131.
[9] 都晓伟,刘艳艳,李滨.从化学和药理学的角度探讨人参、西洋参和三七的传统应用[J].中医药学报,2005,33(4):66-69.
[10] 钟薇,戴雨霖,李晓宇,等.超高效液相色谱-四极杆-静电场轨道阱质谱法分析不同炮制红参的化学成分差异[J].质谱学报,2015,36(6):529-534.
[11] 张聪,王智华,金德庄.中国红参与高丽红参的指纹谱(HPLC-FPS)比较研究[J].中成药,2001,23(3):160-163.
[12] 沈志滨,朱俊访,李博.HPLC法测定益气复脉口服液中人参皂苷Rg1、Re、Rb1的含量[J].广东药学院学报,2006,22(3):259,267.
[13] 郑重,宋凤瑞,刘淑莹,等.人参、红参皂苷类成分指纹图谱研究[J].质谱学报,2012,33(6):327-333.
[14] 孙国祥,张静娴.基于三波长融合谱的系统指纹定量法鉴定龙胆泻肝丸的真实质量[J].色谱,2009,27(3):318-322.
[15] 花汝凤,黄可儿,柯雪红,等.补中益气汤双波长融合指纹图谱的整体性分析[J].中药新药与临床药理,2008,19(1):42-47.
[16] 王京辉,陈晶,郭洪祝,等.进口红参与国产红参皂苷类成分含量及鉴别成分研究[J].中国药学杂志,2018,53(13):1070-1076.