摘要
[目的]分离筛选获得能高效净化污水的净化细菌。[方法]利用氨氮降解筛选培养基对某污水处理厂的活性污泥进行净水细菌的分离筛选,并研究分离菌株对污水有机质、氨态氮、总磷与总氮含量的去除效果以及药敏性分析。[结果]比较去除效果,获得了1株最高效的净水细菌,该菌株对污水有机质、氨态氮、总氮与总磷均具有较强的净化效果,其去除率分别达58. 90%、65. 45%、51. 91%和35. 00%。结合菌落形态特征观察、生理生化分析及分子鉴定,鉴定该菌株为暹罗芽胞杆菌(Bacillus siamensis)。该菌株对7种常见抗生素均具有一定敏感性,其中对氨苄青霉素最敏感,卡那霉素次之,对庆大霉素最不敏感。[结论]分离筛选获得1株能高效净化污水的暹罗芽胞杆菌,对常见抗生素具有一定敏感性。
[Objective] The purified bacteria capable of purifying sewage efficiently were obtained by separation and screening.[Method] The ammonia sludge degradation screening medium was used to separate and filter the activated sludge from a sewage treatment plant,and the removal effect of the isolates on the organic matter,ammonia nitrogen,total phosphorus and total nitrogen content and the sensitivity of the drug were analyzed.[Result]A most efficient water-purifying bacteria was obtained by comparison of the removal effects. The strain had a strong purification effect on the organic matter,ammonia nitrogen,total nitrogen and tolal phosphorus of the sewage,and the removal rates were 58. 90%,65. 45%,51. 91% and 35. 00%. Combined with colony morphological observation,physiological and biochemical analysis and molecular identification,the strain was identified as Bacillus siamensis. The strain was sensitive to seven common antibiotics,among which ampicillin was the most sensitive,kanamycin was the second,and gentamicin was the least sensitive.[Conclusion] Separation and screening obtained a strain of Bacillus siamensis that can effectively purify sewage,and had certain sensitivity to common antibiotics.
引文
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