补中益气汤对Lewis肺癌小鼠EGFR、NF-κB、ICAM-1表达及JAK1、STAT1蛋白磷酸化的影响
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摘要
目的:探讨补中益气汤对Lewis肺癌小鼠表皮生长因子(epidermal growth factor receptor,EGFR),核转录因子κB(nuclear factor kappa beta,NF-κB),细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)表达及蛋白质酪氨酸激酶-1(janus kinase-1,JAK1),转录活化蛋白1(signal transducer and activator of transcription 1,STAT1)蛋白磷酸化的影响。方法:选取SPF级小鼠60只,随机分为空白组、模型组、西药组、补中益气汤高剂量组、补中益气汤中剂量组、补中益气汤低剂量组,每组10只。除空白组外均将Lewis肺癌细胞无菌接种于小鼠的右腋皮下,建立Lewis肺癌模型。造模成功后,模型组以无菌生理盐水0.2 m L灌胃,西药组小鼠给予吉非替尼50 mg·kg~(-1),每次0.2 m L;补中益气汤高、中、低剂量组以补中益气汤20 g·kg~(-1)、10 g·kg~(-1)、5 g·kg~(-1)进行灌胃,每次0.2 m L,灌胃均为每天1次。各组用药2周。观察并比较各组小鼠的瘤质量、瘤指数、NF-κB、ICAM-1、EGFR、JAK1、STAT1水平。结果:与模型组相比,西药组、补中益气汤高剂量组、补中益气汤中剂量组小鼠的瘤质量和瘤指数,NF-κB、ICAM-1、EGFR及JAK1、STAT1水平较低,差异具有统计学意义(P<0.05);与西药组相比,补中益气汤高剂量组、补中益气汤中剂量组小鼠的瘤质量和瘤指数,NF-κB、ICAM-1、EGFR、JAK1、STAT1水平较低,差异具有统计学意义(P<0.05);补中益气汤高剂量组、补中益气汤中剂量组小鼠的瘤质量和瘤指数,NF-κB、ICAM-1、EGFR、JAK1、STAT1水平相比,差异无统计学意义(P>0.05)。结论:补中益气汤能够降低Lewis肺癌小鼠瘤质量和瘤指数,降低EGFR,NF-κB、ICAM-1、JAK1、STAT1水平,对临床具有一定的参考意义。
Objective:To investigate the effects of Buzhong Yiqi Decoction on epidermal growth factor receptor( EGFR),nuclear factor kappa beta( NF-κB),cell adhesion molecule-1( ICAM-1) and phosphorylation of protein tyrosine kinase-1( JAK1) and STAT1 protein( STAT1) in Lewis lung cancer mice. Methods:60 SPF mice were randomly divided into blank group,model group,western medicine group,Buzhong Yiqi Decoction high dose group,Buzhong Yiqi Decoction group,and Buzhong Yiqi Decoction low dose group,with 10 rats in each group. Except for the blank group,Lewis lung cancer cells were established aseptically inoculated into the right iliac crest of mice and a lung cancer tumor model was established. After the model was successfully established,the model group was given 0. 2 m L of sterile normal saline. The western medicine group mice were treated with gefitinib( CTX) 50 mg·kg~(-1),0. 2 m L each time;Buzhong Yiqi Decoction high,middle,and The low-dose group was given intragastric administration of Buzhong Yiqi Decoction at 20 g·kg~(-1),10 g·kg~(-1),5 g·kg~(-1) each time,0. 2 m L each time,and the intragastric administration was performed once daily. The two groups used medication for 2 weeks. The tumor mass,tumor index,NF-κB,ICAM-1,EGFR and JAK1,STAT1 levels were observed and compared in each group of mice. Results:Compared with the model group,the tumor quality and tumor index levels of the mice in the Western medicine group,the high-dose group of Buzhong Yiqi Decoction,and the middle-dose group of Buzhong Yiqi Decoction,NF-κB,ICAM-1,EGFR,and JAK1 The level of STAT1 was lower,and the difference was statistically significant( P < 0. 05). Compared with the western medicine group,the tumor quality and tumor index level of the high-dose Buzhong Yiqi Decoction group and the middle-dose group of Buzhong Yiqi Decoction. The levels of NF-κB,ICAM-1,EGFR and JAK1,STAT1 were lower,the difference was statistically significant( P < 0. 05); the tumor quality of mice in high dose of Buzhong Yiqi Decoction and middle dose of Buzhong Yiqi Decoction was significant The tumor index level,NF-κB,ICAM-1,EGFR,JAK1,and STAT1 levels had no significant difference( P > 0. 05). Conclusion:Buzhong Yiqi Decoction can reduce the tumor quality and tumor index of Lewis lung cancer mice,and decrease the levels of EGFR,NF-κB,ICAM-1,JAK1 and STAT1.
Objective:To investigate the effects of Buzhong Yiqi Decoction on epidermal growth factor receptor( EGFR),nuclear factor kappa beta( NF-κB),cell adhesion molecule-1( ICAM-1) and phosphorylation of protein tyrosine kinase-1( JAK1) and STAT1 protein( STAT1) in Lewis lung cancer mice. Methods:60 SPF mice were randomly divided into blank group,model group,western medicine group,Buzhong Yiqi Decoction high dose group,Buzhong Yiqi Decoction group,and Buzhong Yiqi Decoction low dose group,with 10 rats in each group. Except for the blank group,Lewis lung cancer cells were established aseptically inoculated into the right iliac crest of mice and a lung cancer tumor model was established. After the model was successfully established,the model group was given 0. 2 m L of sterile normal saline. The western medicine group mice were treated with gefitinib( CTX) 50 mg·kg~(-1),0. 2 m L each time;Buzhong Yiqi Decoction high,middle,and The low-dose group was given intragastric administration of Buzhong Yiqi Decoction at 20 g·kg~(-1),10 g·kg~(-1),5 g·kg~(-1) each time,0. 2 m L each time,and the intragastric administration was performed once daily. The two groups used medication for 2 weeks. The tumor mass,tumor index,NF-κB,ICAM-1,EGFR and JAK1,STAT1 levels were observed and compared in each group of mice. Results:Compared with the model group,the tumor quality and tumor index levels of the mice in the Western medicine group,the high-dose group of Buzhong Yiqi Decoction,and the middle-dose group of Buzhong Yiqi Decoction,NF-κB,ICAM-1,EGFR,and JAK1 The level of STAT1 was lower,and the difference was statistically significant( P < 0. 05). Compared with the western medicine group,the tumor quality and tumor index level of the high-dose Buzhong Yiqi Decoction group and the middle-dose group of Buzhong Yiqi Decoction. The levels of NF-κB,ICAM-1,EGFR and JAK1,STAT1 were lower,the difference was statistically significant( P < 0. 05); the tumor quality of mice in high dose of Buzhong Yiqi Decoction and middle dose of Buzhong Yiqi Decoction was significant The tumor index level,NF-κB,ICAM-1,EGFR,JAK1,and STAT1 levels had no significant difference( P > 0. 05). Conclusion:Buzhong Yiqi Decoction can reduce the tumor quality and tumor index of Lewis lung cancer mice,and decrease the levels of EGFR,NF-κB,ICAM-1,JAK1 and STAT1.
引文
[1]张文远,孟令玉,李欣.补中益气汤含药血清对Lewis肺癌细胞增殖和凋亡的影响[J].中医学报,2015,30(4):463-465.
[2]金学敏.益气养阴化瘀法对Lewis肺癌小鼠凋亡相关因子Caspase-3影响研究[D].长春:长春中医药大学,2015.
[3]包素珍,郑小伟,宋红,等.十全大补汤对小鼠Lewis肺癌转移及肿瘤细胞周期的影响[J].中华中医药学刊,2010,28(12):2479-2481.
[4]庄喜兵,乔田奎,许国雄,等.放射抗拒Lewis肺癌细胞系的建立[J].中华放射医学与防护杂志,2015,35(11):805-808.
[5]陈超,陈慧彬,滕鸣健,等.扶正散结汤拆方对Lewis肺癌移植瘤MVD、HIF-1α及COX-2的影响[J].世界中西医结合杂志,2015,10(4):483-486.
[6]王维,段碧霞,曾丽.PARP抑制剂对Lewis肺癌细胞及移植瘤放疗增敏作用及其机制[J].中国肺癌杂志,2016,19(1):16-23.
[7]李小雪,邵鑫,霍永旭,等.MTHF对C57BL/6鼠Lewis肺癌细胞生长的影响[J].四川动物,2015,34(5):729-733.
[8]高亭,蒋引娣,刘小伟,等.羟基红花黄色素A对Lewis肺癌移植瘤微血管密度及血管内皮生长因子mRNA表达的影响[J].中国呼吸与危重监护杂志,2016,15(5):461-464.
[9]夏丹,李勇敏,谭小宁,等.肺复康对Lewis肺癌小鼠肿瘤生长及E-cadherin、Vimentin和TGF-β表达的影响[J].湖南中医药大学学报,2015,35(3):30-33.
[10]谢雄,谢斌,饶斌,等.清燥救肺汤对Lewis肺癌小鼠EGFR,NF-κB,ICAM-1表达及JAK1,STAT1蛋白磷酸化的影响[J].中国实验方剂学杂志,2016,22(24):140-144.
[11]杨国良,胡丹丹.益气养阴方影响Lewis肺癌移植小鼠肿瘤生长的实验研究[J].中华全科医学,2016,14(10):1623-1625.
[12]冯立志,刘小虹.健脾补血方对Lewis肺癌小鼠化疗后5-HT和SP及其受体的影响[J].中药新药与临床药理,2016,27(2):180-184.
[13]易鑫,刘炜,葛正行,等.清解益肺汤及胶囊剂抗小鼠Lewis肺癌转移及肿瘤血管生成机制的研究[J].时珍国医国药,2015,26(10):2366-2368.
[14]冯立志,刘小虹.健脾补血方对Lewis肺癌小鼠化疗后EPO、TPO、GM-CSF的影响[J].中药新药与临床药理,2015,26(4):431-434.
[15]葛驰宇,周舟,张君丽.基于M2与OK-432的肿瘤细胞裂解物致敏的树突状细胞疫苗抗小鼠Lewis肺癌作用研究[J].药学与临床研究,2016,24(2):97-102.
[16]郭毅峻,王倩蕾,杨晓华,等.益气养精小复方对Lewis肺癌荷瘤小鼠免疫功能的影响[J].上海中医药大学学报,2015,29(2):48-52.
[17]齐元富,张盈盈,李慧杰,等.扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用及机制研究[J].山东中医杂志,2016,35(1):61-63.
[18]王克强,陶琳,王绪淮.中药复方对小鼠Lewis肺癌的抑制作用及其抑制血管新生机制初步探讨[J].中国中医基础医学杂志,2015,21(6):671-674.
[2]金学敏.益气养阴化瘀法对Lewis肺癌小鼠凋亡相关因子Caspase-3影响研究[D].长春:长春中医药大学,2015.
[3]包素珍,郑小伟,宋红,等.十全大补汤对小鼠Lewis肺癌转移及肿瘤细胞周期的影响[J].中华中医药学刊,2010,28(12):2479-2481.
[4]庄喜兵,乔田奎,许国雄,等.放射抗拒Lewis肺癌细胞系的建立[J].中华放射医学与防护杂志,2015,35(11):805-808.
[5]陈超,陈慧彬,滕鸣健,等.扶正散结汤拆方对Lewis肺癌移植瘤MVD、HIF-1α及COX-2的影响[J].世界中西医结合杂志,2015,10(4):483-486.
[6]王维,段碧霞,曾丽.PARP抑制剂对Lewis肺癌细胞及移植瘤放疗增敏作用及其机制[J].中国肺癌杂志,2016,19(1):16-23.
[7]李小雪,邵鑫,霍永旭,等.MTHF对C57BL/6鼠Lewis肺癌细胞生长的影响[J].四川动物,2015,34(5):729-733.
[8]高亭,蒋引娣,刘小伟,等.羟基红花黄色素A对Lewis肺癌移植瘤微血管密度及血管内皮生长因子mRNA表达的影响[J].中国呼吸与危重监护杂志,2016,15(5):461-464.
[9]夏丹,李勇敏,谭小宁,等.肺复康对Lewis肺癌小鼠肿瘤生长及E-cadherin、Vimentin和TGF-β表达的影响[J].湖南中医药大学学报,2015,35(3):30-33.
[10]谢雄,谢斌,饶斌,等.清燥救肺汤对Lewis肺癌小鼠EGFR,NF-κB,ICAM-1表达及JAK1,STAT1蛋白磷酸化的影响[J].中国实验方剂学杂志,2016,22(24):140-144.
[11]杨国良,胡丹丹.益气养阴方影响Lewis肺癌移植小鼠肿瘤生长的实验研究[J].中华全科医学,2016,14(10):1623-1625.
[12]冯立志,刘小虹.健脾补血方对Lewis肺癌小鼠化疗后5-HT和SP及其受体的影响[J].中药新药与临床药理,2016,27(2):180-184.
[13]易鑫,刘炜,葛正行,等.清解益肺汤及胶囊剂抗小鼠Lewis肺癌转移及肿瘤血管生成机制的研究[J].时珍国医国药,2015,26(10):2366-2368.
[14]冯立志,刘小虹.健脾补血方对Lewis肺癌小鼠化疗后EPO、TPO、GM-CSF的影响[J].中药新药与临床药理,2015,26(4):431-434.
[15]葛驰宇,周舟,张君丽.基于M2与OK-432的肿瘤细胞裂解物致敏的树突状细胞疫苗抗小鼠Lewis肺癌作用研究[J].药学与临床研究,2016,24(2):97-102.
[16]郭毅峻,王倩蕾,杨晓华,等.益气养精小复方对Lewis肺癌荷瘤小鼠免疫功能的影响[J].上海中医药大学学报,2015,29(2):48-52.
[17]齐元富,张盈盈,李慧杰,等.扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用及机制研究[J].山东中医杂志,2016,35(1):61-63.
[18]王克强,陶琳,王绪淮.中药复方对小鼠Lewis肺癌的抑制作用及其抑制血管新生机制初步探讨[J].中国中医基础医学杂志,2015,21(6):671-674.