外源H_2O_2通过介导抗氧化酶、ABA和GA促进高盐胁迫下黄瓜种子的萌发
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摘要
为了探讨外源H_2O_2对盐胁迫下黄瓜种子萌发抑制作用的缓解机制,以黄瓜品种‘津瑞100’种子为材料,采用培养皿滤纸发芽法,测定了不同浓度H_2O_2浸种处理对150 mmol·L~(-1) NaCl胁迫下黄瓜种子萌发及相关生理指标的影响。结果表明,在150 mmol·L~(-1) NaCl胁迫下,黄瓜种子的萌发受明显抑制, MDA含量显著增加,抗氧化酶(SOD、POD、CAT、APX)的活性降低; ABA含量显著增加,而GA含量显著降低。适宜浓度的H_2O_2浸种处理能不同程度提高盐胁迫下黄瓜种子的萌发率和抗氧化酶(SOD、POD、CAT、APX)的活性;降低MDA和ABA含量,增加GA含量,从而缓解盐胁迫对黄瓜种子萌发的抑制作用。其中以0.3%H_2O_2浸种处理效果最好。高浓度H_2O_2处理对盐胁迫的缓解作用逐渐减弱甚至加重其毒害效应。说明适宜浓度的H_2O_2可以通过介导抗氧化酶系统、ABA和GA缓解盐胁迫对黄瓜种子萌发的抑制作用。
In order to explore the ameliorating mechanism of hydrogen peroxide(H_2O_2) on inhibition of seed germination of cucumber caused by NaCl, the effects of different H_2O_2 concentration on seed germination and physiological characteristics of cucumber(‘Jinrui No. 100’) were studied under 150 mmol·L~(-1) NaCl stress. The results showed that the seed germination of cucumber was inhibited significantly by 150 mmol·L~(-1) NaCl stress. MDA content increased significantly, the activities of SOD, POD, CAT and APX were inhibited obviously. On the other hand, ABA content increased significantly, GA content decreased obviously. Suitable concentration of H_2O_2 could alleviate the inhibition effects of NaCl. Compared to 150 mmol·L~(-1) NaCl stress, the seed germination percent, the activities of SOD, POD, CAT and APX, and GA content could be improved significantly by pretreated with 0.3% H_2O_2, while ABA content significantly decreased. Moreover, we found that the mitigation effect of NaCl stress was gradually weakened with the increase of H_2O_2 concentration, and 1% H_2O_2 would aggravate the toxicity of NaCl stress. In this study, we found that the appropriate concentration of H_2O_2 could alleviate the inhibitory effects of NaCl stress on germination mainly by regulating antioxidant systems, ABA and GA interaction in cucumber.
In order to explore the ameliorating mechanism of hydrogen peroxide(H_2O_2) on inhibition of seed germination of cucumber caused by NaCl, the effects of different H_2O_2 concentration on seed germination and physiological characteristics of cucumber(‘Jinrui No. 100’) were studied under 150 mmol·L~(-1) NaCl stress. The results showed that the seed germination of cucumber was inhibited significantly by 150 mmol·L~(-1) NaCl stress. MDA content increased significantly, the activities of SOD, POD, CAT and APX were inhibited obviously. On the other hand, ABA content increased significantly, GA content decreased obviously. Suitable concentration of H_2O_2 could alleviate the inhibition effects of NaCl. Compared to 150 mmol·L~(-1) NaCl stress, the seed germination percent, the activities of SOD, POD, CAT and APX, and GA content could be improved significantly by pretreated with 0.3% H_2O_2, while ABA content significantly decreased. Moreover, we found that the mitigation effect of NaCl stress was gradually weakened with the increase of H_2O_2 concentration, and 1% H_2O_2 would aggravate the toxicity of NaCl stress. In this study, we found that the appropriate concentration of H_2O_2 could alleviate the inhibitory effects of NaCl stress on germination mainly by regulating antioxidant systems, ABA and GA interaction in cucumber.
引文
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Cai FX,Chen DD,Yang F,et al(2016).Regulation of H2O2literal on growth and physiology of rice seedlings.Jiangsu Agr Sci,44:74-77(in Chinese)[蔡凤香,陈豆豆,杨飞等(2016).H2O2对水稻幼苗生长和生理的调节.江苏农业科学,44:74-77]
Chen ML,Huang YQ,Liu JQ,et al(2011).Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flightmass spectrometry.J Chromatogr B,879:938-944
Etehadnia M,Waterer D,Jong HD,et al(2008).Scion and rootstock effects on ABA-mediated plant growth regulation and salt tolerance of acclimated and unacclimated potato genotypes.J Plant Growth Regul,27:125-140
Fedina IS,Nedeva D,?i?ek N(2009).Pre-treatment with H2O2 induces salt tolerance in barley seedlings.Biol Plant,53:321-324
Halliwell B,Grootveld M,Gutteridge JMC(1988).Methods for the measurement of hydroxyl radicals in biomedical systems:deoxyribose degradation and aromatic hydroxylation.Methods Biochem Anal,33:59-90
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Kucera B,Cohn MA,Leubner-Metzger G(2005).Plant hormone interactions during seed dormancy release and germination.Seed Sci Res,15:281-307
Li SW,Xue LG,Xu SJ,et al(2009).Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings.Environ Exp Bot,65:63-71
Liu YG,Ye NH,Liu R,et al(2010).H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.J Exp Bot,61:2979-2990
Schopfer P,Plachy C,Frahry G(2001).Release of reactive oxygen intermediates(superoxide radicals,hydrogen peroxide,and hydroxyl radicals)and peroxidase in germinating radish seeds controlled by light,gibberellin,and abscisic acid.Plant Physiol,125:1591-1602
Sun ZH,Fan HF,Du CX,et al(2016).NaCl stress on antioxidant enzyme isozymes expressed in cucumber seedling leaves,phloem exudates,and roots.J Zhejiang A&FUniv,33:652-657(in Chinese with English abstract)[孙张晗,樊怀福,杜长霞等(2016).盐胁迫对黄瓜幼苗叶片、韧皮部渗出液和根系抗氧化酶同工酶表达的影响.浙江农林大学学报,33:652-657]
Uchida A,Jagendorf AT,Takabe T,et al(2002).Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice.Plant Sci,163:515-523
Yang B,He JY,Ren YF,et al(2018).Alleviating effect of hydrogen peroxide on seed germination of rice under cadmium stresss.Plant Physiol J,54(6):1111-1118(in Chinese with English abstract)[杨波,何俊瑜,任艳芳等(2018).过氧化氢对镉胁迫下水稻种子萌发的缓解效应.植物生理学报,54(6):1111-1118]
Yang XL,Yu JH,Li YJ,et al(2004)Effects of NaCl stress on seed germination and seedling growth of Cucumis sativus.J Gansu Agr Univ,39:6-9(in Chinese with English abstract)[杨秀玲,郁继华,李雅佳等(2004).NaCl胁迫对黄瓜种子萌发及幼苗生长的影响.甘肃农业大学学报,39:6-9]
Zhang JY,Wu FZ(2007).Effects of salt stress membrane lipid peroxidation and proline content in cucumber cultivars.Chin Veg,7:12-15(in Chinese with English abstract)[张景云,吴凤芝(2007).盐胁迫对黄瓜不同耐盐品种膜脂过氧化及脯氨酸含量的影响.中国蔬菜,7:12-15]
Zhang ZL,Qu WJ(2003).Plant Physiology Instruction.Beijing:Higher Education Press,274-277[张志良,瞿伟菁(2003).植物生理学指导.北京:高等教育出版社,274-277]
Zhou GQ,He JY,Ren YF,et al(2009).Alleviating effects of Pr3+on the cadmium stress in rice seeds germination.Acta Agri Boreali Sin,24:112-116(in Chinese with English abstract)[周国强,何俊瑜,任艳芳等(2009).硝酸镨对镉胁迫下水稻种子萌发的缓解效应.华北农学报,24:112-116]
Brennan T,Frenkel C(1977).Involvement of hydrogen-peroxide in regulation of senescence in pear.Plant Physiol,59:411-416
Cai FX,Chen DD,Yang F,et al(2016).Regulation of H2O2literal on growth and physiology of rice seedlings.Jiangsu Agr Sci,44:74-77(in Chinese)[蔡凤香,陈豆豆,杨飞等(2016).H2O2对水稻幼苗生长和生理的调节.江苏农业科学,44:74-77]
Chen ML,Huang YQ,Liu JQ,et al(2011).Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flightmass spectrometry.J Chromatogr B,879:938-944
Etehadnia M,Waterer D,Jong HD,et al(2008).Scion and rootstock effects on ABA-mediated plant growth regulation and salt tolerance of acclimated and unacclimated potato genotypes.J Plant Growth Regul,27:125-140
Fedina IS,Nedeva D,?i?ek N(2009).Pre-treatment with H2O2 induces salt tolerance in barley seedlings.Biol Plant,53:321-324
Halliwell B,Grootveld M,Gutteridge JMC(1988).Methods for the measurement of hydroxyl radicals in biomedical systems:deoxyribose degradation and aromatic hydroxylation.Methods Biochem Anal,33:59-90
Han N,Feng HQ,Li YH,et al(2009).Regulation of exogenous H2O2 on cold resistance of rice seedlings.Acta Bot Boreali-Occident Sin,29:1214-1219(in Chinese with English abstract)[韩娜,冯汉青,李燕鸿等(2009).外源H2O2对水稻幼苗抗寒性的调节作用.西北植物学报,29:1214-1219]
Kucera B,Cohn MA,Leubner-Metzger G(2005).Plant hormone interactions during seed dormancy release and germination.Seed Sci Res,15:281-307
Li SW,Xue LG,Xu SJ,et al(2009).Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings.Environ Exp Bot,65:63-71
Liu YG,Ye NH,Liu R,et al(2010).H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.J Exp Bot,61:2979-2990
Schopfer P,Plachy C,Frahry G(2001).Release of reactive oxygen intermediates(superoxide radicals,hydrogen peroxide,and hydroxyl radicals)and peroxidase in germinating radish seeds controlled by light,gibberellin,and abscisic acid.Plant Physiol,125:1591-1602
Sun ZH,Fan HF,Du CX,et al(2016).NaCl stress on antioxidant enzyme isozymes expressed in cucumber seedling leaves,phloem exudates,and roots.J Zhejiang A&FUniv,33:652-657(in Chinese with English abstract)[孙张晗,樊怀福,杜长霞等(2016).盐胁迫对黄瓜幼苗叶片、韧皮部渗出液和根系抗氧化酶同工酶表达的影响.浙江农林大学学报,33:652-657]
Uchida A,Jagendorf AT,Takabe T,et al(2002).Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice.Plant Sci,163:515-523
Yang B,He JY,Ren YF,et al(2018).Alleviating effect of hydrogen peroxide on seed germination of rice under cadmium stresss.Plant Physiol J,54(6):1111-1118(in Chinese with English abstract)[杨波,何俊瑜,任艳芳等(2018).过氧化氢对镉胁迫下水稻种子萌发的缓解效应.植物生理学报,54(6):1111-1118]
Yang XL,Yu JH,Li YJ,et al(2004)Effects of NaCl stress on seed germination and seedling growth of Cucumis sativus.J Gansu Agr Univ,39:6-9(in Chinese with English abstract)[杨秀玲,郁继华,李雅佳等(2004).NaCl胁迫对黄瓜种子萌发及幼苗生长的影响.甘肃农业大学学报,39:6-9]
Zhang JY,Wu FZ(2007).Effects of salt stress membrane lipid peroxidation and proline content in cucumber cultivars.Chin Veg,7:12-15(in Chinese with English abstract)[张景云,吴凤芝(2007).盐胁迫对黄瓜不同耐盐品种膜脂过氧化及脯氨酸含量的影响.中国蔬菜,7:12-15]
Zhang ZL,Qu WJ(2003).Plant Physiology Instruction.Beijing:Higher Education Press,274-277[张志良,瞿伟菁(2003).植物生理学指导.北京:高等教育出版社,274-277]
Zhou GQ,He JY,Ren YF,et al(2009).Alleviating effects of Pr3+on the cadmium stress in rice seeds germination.Acta Agri Boreali Sin,24:112-116(in Chinese with English abstract)[周国强,何俊瑜,任艳芳等(2009).硝酸镨对镉胁迫下水稻种子萌发的缓解效应.华北农学报,24:112-116]