简化样品处理快速测定酸枣仁中多种成分含量的研究
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摘要
目的:建立简化样品处理快速测定酸枣仁中多种化学成分含量的方法。方法:比较酸枣仁样品的不同前处理方法,包括简单超声提取法、直接加热回流法和脱脂后加热回流提取法(药典方法)下UPLC-UV-ELSD液相检测黄酮类和皂苷类等4种成分的峰面积。结果:通过比较,药典方法在酸枣仁脱脂后回流4h反而导致成分含量降低,加热回流30 min提取率最高,以此对比,超声90 min对酸枣仁中皂苷类和黄酮类成分的提取效果相当,提取溶剂为70%乙醇,物料比为1∶30。结论:本文建立的提取方法可以获得提取充分的效果,且快速和易于操作,为控制酸枣仁中黄酮类成分和皂苷类成分含量的快速测定提供了可靠的方法。
Objective:To establish a simple and rapid method for the determination of various chemical components.Methods:Through UPLC-UV-ELSD liquid phase method spinosin,6-feruloyl spinosin,jujuboside A and jujuboside B 4 kinds of ingredients such as peak area,different pretreatment methods were compared for the samples of spina date seed,including simple ultrasonic extraction,direct heating reflux and degreased and then heated reflux extraction.Results:By comparison,the reflux of 4 hours after the degreasing of Semen Ziziphi Spinosae resulted in the decrease of the content of the ingredients in Semen Ziziphi Spinosae.Heating reflux for 30 min was the best extraction method.By contrast,The effect of ultrasound at 90 min was comparable to that of reflux at 30 min.Therefore,the ultrasonic extraction method was recommended for 90 min.In ultrasonic extraction,the final optimum extraction condition were 70% ethanol as the extracting solvent,1∶30 as the material ratio.Conclusion:The method established in this paper can obtain sufficient extraction effect,and is fast and easy to operate.It provides a reliable method for rapid determination of flavonoids and saponins in Semen Ziziphi Spinosae.
Objective:To establish a simple and rapid method for the determination of various chemical components.Methods:Through UPLC-UV-ELSD liquid phase method spinosin,6-feruloyl spinosin,jujuboside A and jujuboside B 4 kinds of ingredients such as peak area,different pretreatment methods were compared for the samples of spina date seed,including simple ultrasonic extraction,direct heating reflux and degreased and then heated reflux extraction.Results:By comparison,the reflux of 4 hours after the degreasing of Semen Ziziphi Spinosae resulted in the decrease of the content of the ingredients in Semen Ziziphi Spinosae.Heating reflux for 30 min was the best extraction method.By contrast,The effect of ultrasound at 90 min was comparable to that of reflux at 30 min.Therefore,the ultrasonic extraction method was recommended for 90 min.In ultrasonic extraction,the final optimum extraction condition were 70% ethanol as the extracting solvent,1∶30 as the material ratio.Conclusion:The method established in this paper can obtain sufficient extraction effect,and is fast and easy to operate.It provides a reliable method for rapid determination of flavonoids and saponins in Semen Ziziphi Spinosae.
引文
[1] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:366-367.
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[5] 祝洪艳,张力娜,唐珊,等.HPLC法测定3个产地酸枣仁中斯皮诺素和酸枣仁皂苷A、B的含量[J].药物分析杂志,2015,35(12):2099-2014.
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[7] 李铮,任爽,常增荣,等.中药酸枣仁饮片中3种成分的含量比较研究[J].中国新药杂志,2017,26(1):91-96.
[8] 张雪,向瑞平.酸枣仁中皂苷A的含量测定研究[J].现代牧业,2010,30(3):7-9.
[9] 马玲云,吴朝阳,马双成.RP-HPLC-ELSD法同时测定中药及保健食品中酸枣仁皂苷A和B含量的方法验证[J].临床合理用药杂志,2010,3(7):37-38.
[10] 张巧月,杨浩天,史贺,等.HPLC-MS法同时测定酸枣仁中9种成分[J].中草药,2015,46(1):123-127.
[2] 隋利强,王建,黄礼书,等.蜜炙酸枣仁中斯皮诺素含量测定[J].世界中医药,2016,11(6):1085-1087.
[3] 崔思娇,罗洁,张靓琦,等.UPLC同时测定酸枣仁中4种活性成分的含量[J].世界科学技术-中医药现代化,2013,15(6):1323-1328.
[4] 闫艳,杜晨辉,李小菊,等.HPLLC-DAD-ELSD法同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B的含量[J].药物分析杂志,2011,31(1):30-33.
[5] 祝洪艳,张力娜,唐珊,等.HPLC法测定3个产地酸枣仁中斯皮诺素和酸枣仁皂苷A、B的含量[J].药物分析杂志,2015,35(12):2099-2014.
[6] ZHANG M,ZHANG Y,XIE J.Simultaneous determination of jujuboside A,B and betulinic acid in semen Ziziphi spinosae by high performance liquid chromatography-evaporative light scattering detection[J].J Pharm Biomed Anal,2008,48(5):1467-1470.
[7] 李铮,任爽,常增荣,等.中药酸枣仁饮片中3种成分的含量比较研究[J].中国新药杂志,2017,26(1):91-96.
[8] 张雪,向瑞平.酸枣仁中皂苷A的含量测定研究[J].现代牧业,2010,30(3):7-9.
[9] 马玲云,吴朝阳,马双成.RP-HPLC-ELSD法同时测定中药及保健食品中酸枣仁皂苷A和B含量的方法验证[J].临床合理用药杂志,2010,3(7):37-38.
[10] 张巧月,杨浩天,史贺,等.HPLC-MS法同时测定酸枣仁中9种成分[J].中草药,2015,46(1):123-127.