干旱高温互作下高山杜鹃幼苗生理生化变化研究
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摘要
采取模拟干旱和高温交叉胁迫方法,测定干旱高温互作下高山杜鹃幼苗的生理指标并进行分析,为耐干旱高温种质的遗传改良和人工引种栽培提供理论依据。结果表明,随着干旱高温时间的延长,在40℃高温下,20%的PEG胁迫对高山杜鹃幼苗的伤害程度最大。在干旱高温互作下,脯氨酸含量呈上升趋势,其含量平均高达对照组的283.3%;MDA含量和相对电导率分别比对照组增加了178.1%和163.8%;可溶性蛋白比对照组增加了28.8%;POD、CAT的活性呈上升趋势,分别比对照组上升了71.9%和22.9%;而SOD活性呈先升后降趋势,与对照组相比增加了15.4%,说明干旱高温互作对高山杜鹃幼苗的生长和干旱高温抗性均有显著影响。
In order to provide a theoretical basis for the genetic improvement and artificial introduction and cultivation of Rhododendron lapponicum germplasm resources tolerant to drought and high temperature, we investigated the effects of drought and high temperature interaction on the physiological and biochemical indexes of R. lapponicum seedlings by adopting the method of simulating drought and high temperature stress. The results showed that: along with the extension of drought and high temperature(40 ℃) stress time, 20% PEG stress had the greatest injury to R. lapponicum seedlings. Under drought and high temperature interaction, the proline content in R. lapponicum seedlings increased, being 283.3% that of the control group in average; the MDA content and the relative conductivity increased 178.1% and 163.8% that in the control group, respectively; the content of soluble protein was 0.36 mg/g higher than that in the control group; the POD and CAT activities exhibited a rising trend, and were 71.9% and 22.9% higher than that in the control group, separately; while the activity of SOD increased first and then decreased, and was increased by 15.4%. Thus, drought and high temperature interaction had significant effects on the growth and resistance of R. lapponicum seedlings.
In order to provide a theoretical basis for the genetic improvement and artificial introduction and cultivation of Rhododendron lapponicum germplasm resources tolerant to drought and high temperature, we investigated the effects of drought and high temperature interaction on the physiological and biochemical indexes of R. lapponicum seedlings by adopting the method of simulating drought and high temperature stress. The results showed that: along with the extension of drought and high temperature(40 ℃) stress time, 20% PEG stress had the greatest injury to R. lapponicum seedlings. Under drought and high temperature interaction, the proline content in R. lapponicum seedlings increased, being 283.3% that of the control group in average; the MDA content and the relative conductivity increased 178.1% and 163.8% that in the control group, respectively; the content of soluble protein was 0.36 mg/g higher than that in the control group; the POD and CAT activities exhibited a rising trend, and were 71.9% and 22.9% higher than that in the control group, separately; while the activity of SOD increased first and then decreased, and was increased by 15.4%. Thus, drought and high temperature interaction had significant effects on the growth and resistance of R. lapponicum seedlings.
引文
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[21] 谢亚军,王兵,梁新华,等.干旱胁迫对甘草幼苗活性氧代谢及保护酶活性的影响[J].农业科学研究,2008,29(4):19-22.
[22] Wang X,Peng Y H,Singer J W,et al.Seasonal changes in photosynthesis,antioxidant systems and ELIP experssion in a thermonastic and non-thermonastic Rhododendron species:A compare is on of photoprotective strategies in overwintering plants[J].Plant Science,2009,177(6):607-617.
[23] 时忠杰,杜阿朋,胡哲森,等.水分胁迫对板栗幼苗叶片活性氧代谢的影响[J].林业科学研究,2007,20(5):683-687.
[24] 姬承东,陈平,朱钧,等.高温对匍匐翦股颖果岭草坪草生理特性及再生性的影响[J].中国农学通报,2007,23(1):221-224.
[25] 王丽娟,欧静,钟登慧,等.高温胁迫对桃叶杜鹃幼苗生理生化指标的响应[J].湖北农业科学,2014,53(20):4882-4884.
[2] Ghouil H,Montpied P,Epron D,et al.Thermal optima of photosynthetic functions and thermostability of photochemisity in cork oak seedlings[J].Tree Physiology,2003,23(15):1031-1039.
[3] 赵冰,杜宇科,付玉梅,等.镇安木王国家森林公园野生杜鹃花资源调查[J].Journal of Landscape Research,2010,2(1):54-56,61.
[4] Wu Z Y,Raven P H,Hong D Y.Flora of China (Volum 14) [M].Beijing:Science Press,2005:260-455.
[5] 赵冰,杜宇科,付玉梅,等.镇安木王国家森林公园野生杜鹃花资源调查[J].安徽农业科学,2010,38(8):4000-4001,4041.
[6] 李璟琦,苏真,张晓静.秦岭杜鹃花属植物资源及其利用研究[J].中国农学通报,2012,28(22):304-307.
[7] 赵冰,张果,司国臣,等.秦岭野生杜鹃花属植物种质资源调查研究[J].西北林学院报,2013,28(1):104-109.
[8] Marian C O,Krebs S L,Arora R.Dehydrin variability among Rhododendron species:a 25 kDa dehydrin is conserved and associated with cold acclimation across diverse species[J].New Phytologist,2003,16(3):773-780.
[9] 余丽玲,何天友,陈凌艳,等.人工低温胁迫下西洋杜鹃生理生化指标的变化[J].农学学报,2014,4(1):48-53.
[10] 庞新华,罗清.杜鹃耐热生理研究进展[J].北方园艺,2016(13):192-195.
[11] 李娟,黄丽华,陈训.2种杜鹃对干旱胁迫的生理响应及抗旱性评价[J].西南农业学报,2015,28(3):1067-1072.
[12] 李小方.植物生理学实验指导[M].北京:高等教育出版社,2016:191-192.
[13] 陈建勋,王晓峰.植物生理学实验指导(第二版)[M].广州:华南理工大学出版社,2006:64-66.
[14] 张志良,瞿伟菁.植物生理学实验指导(第四版)[M].北京:高等教育出版社,2009:227-229.
[15] 李合生,植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:167-169.
[16] 郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:110-112.
[17] 高俊风.植物生理学实验指导[M].北京:高等教育出版社,2006:140-143.
[18] 刘世鹏,刘济明,陈宗礼,等.模拟干旱胁迫对枣树幼苗的抗氧化系统和渗透调节的影响[J].西北植物学报,2006,26(9):221-224.
[19] 刘应迪,曹同,向芬,等.高温胁迫下两种藓类植物过氧化物酶活性的变化[J].广西植物,2001,21(3):255-258.
[20] 张显强,罗在柒,唐金刚,等.高温和干旱胁迫对鳞叶藓游离脯氨酸和可溶性糖含量的影响[J].广西植物,2004(6):570-573.
[21] 谢亚军,王兵,梁新华,等.干旱胁迫对甘草幼苗活性氧代谢及保护酶活性的影响[J].农业科学研究,2008,29(4):19-22.
[22] Wang X,Peng Y H,Singer J W,et al.Seasonal changes in photosynthesis,antioxidant systems and ELIP experssion in a thermonastic and non-thermonastic Rhododendron species:A compare is on of photoprotective strategies in overwintering plants[J].Plant Science,2009,177(6):607-617.
[23] 时忠杰,杜阿朋,胡哲森,等.水分胁迫对板栗幼苗叶片活性氧代谢的影响[J].林业科学研究,2007,20(5):683-687.
[24] 姬承东,陈平,朱钧,等.高温对匍匐翦股颖果岭草坪草生理特性及再生性的影响[J].中国农学通报,2007,23(1):221-224.
[25] 王丽娟,欧静,钟登慧,等.高温胁迫对桃叶杜鹃幼苗生理生化指标的响应[J].湖北农业科学,2014,53(20):4882-4884.