不同叶用芥菜品种的抗旱性评价
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摘要
本研究对贵州喀斯特地方4个叶用芥菜品种采用盆栽试验,设置不同程度的干旱胁迫,研究其在不同干旱胁迫下的抗旱性。结果显示,随着干旱胁迫的加重,三大渗透调节物质Pro、SS、SP含量逐渐增大;超氧化物歧化酶(POD)、过氧化物酶(SOD)、过氧化氢酶(CAT)活性随着干旱胁迫加重呈先上升后下降趋势,丙二醛(MDA)也呈逐渐增大趋势。贵州地方4个叶用芥菜品种综合抗旱性评定结果为:2号≧1号>3号>4号。因此本试验研究干旱胁迫对叶用芥菜生理方面的影响,筛选出适合喀斯特地区种植的高抗旱品种,提高芥菜的产量和经济效益,为贵州筛选高抗旱芥菜品种提供一定基础数据。
In this study, pot experiments were conducted on four leaf mustard cultivars in karst region of Guizhou,and different drought stress levels were set up to investigate the drought resistance of the materials under different drought stress. The results showed that with the aggravation of drought stress, the content of three osmotic adjustment substances(Pro, SS and SP) increased gradually. The activities of superoxide dismutase(POD), peroxidase(SOD) and catalase(CAT) increased first and then decreased with the increase of drought stress, while the activities of malondialdehyde(MDA) also increased gradually. The comprehensive drought resistance evaluation results of 4 leaf mustard cultivars in Guizhou area were: No.2 ≥No.1 >No.3 >No.4. Therefore, this experiment was conducted to study the physiological effects of drought stress on leaf mustard, so as to select high drought-resistant varieties suitable for karst area and improve the yield and economic benefits of mustard, which could provide some basic data for the selection of mustard varieties with strong drought resistance in Guizhou.
In this study, pot experiments were conducted on four leaf mustard cultivars in karst region of Guizhou,and different drought stress levels were set up to investigate the drought resistance of the materials under different drought stress. The results showed that with the aggravation of drought stress, the content of three osmotic adjustment substances(Pro, SS and SP) increased gradually. The activities of superoxide dismutase(POD), peroxidase(SOD) and catalase(CAT) increased first and then decreased with the increase of drought stress, while the activities of malondialdehyde(MDA) also increased gradually. The comprehensive drought resistance evaluation results of 4 leaf mustard cultivars in Guizhou area were: No.2 ≥No.1 >No.3 >No.4. Therefore, this experiment was conducted to study the physiological effects of drought stress on leaf mustard, so as to select high drought-resistant varieties suitable for karst area and improve the yield and economic benefits of mustard, which could provide some basic data for the selection of mustard varieties with strong drought resistance in Guizhou.
引文
Bai P.,Ran C.Y.,and Xie X.Y.,2014,Influence of drought stress on physiological characteristics and agronomic traits at bud stage of(Brassica napus L.),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(18):3566-3576(白鹏,冉春艳,谢小玉,2014,干旱胁迫对油菜蕾薹期生理特征及农艺性状的影响,中国农业科学,47(18):3566-3576)
Chen J.,Gao G.Z.,Zhao L.,Xu L.S.,Zhang X.T.,and Xue W.,2015,Effects of wheat seed soaking with chitosan on protective enzymetic activities in germination stage under drought stress,Jiyinzuxue Yu Yingyong Sh engwuxue(Genomics and Applied Biology),35(10):2251-2254(陈军,高贵珍,赵亮,徐礼生,张兴桃,薛文,2015,干旱胁迫下壳聚糖浸种对小麦萌发期保护酶活性的影响,基因组学与应用生物学,35(10):2251-2254)
Chong P.F.,Li H.Y.,and Li Y.,2015,Physiological responses of seedling roots of the desert plant R eaumuria soongorica to drought stress,Caoye Xuebao(Acta Prataculturae Sinica),24(1):72-80(种培芳,李航毅,李毅,2015,荒漠植物红砂根系对干旱胁迫的生理响应,草业学报,24(1):72-80)
Guo Y.P.,Mi F.G.,Yan L.J.,Ren Y.X.,LüS.J.,and Fu B.Z.,2014,Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties,Caoye Xuebao(Acta Prataculturae Sinica),23(4):220-228(郭郁频,米富贵,闫利军,任永霞,吕世杰,伏兵哲,2014,不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价,草业学报,23(4):220-228)
Han R.H.,Tian H.,Gao G.J.,and Lu X.S.,2008,Change of water metabolism and two osmotic adjustment substances in the leaves of alfalfa under drought stress.,Huabei Nongxuebao(Acta Aoriculturae Boreali-sinica),23(4):140-144(韩瑞宏,田华,高桂娟,卢欣石,2008,干旱胁迫下紫花苜蓿叶片水分代谢与两种渗透调节物质的变化,华北农学报,23(4):140-144)
Ji Y.,Zhang X.Q.,Peng Y.,Liang X.Y.,Huang L.K.,Ma X.,and Ma Y.M.,2014,Effects of drought stress on lipid peroxidation,osmotic adjustment and activities of protective enzymes in the roots and leaves of orchardgrass,Caoye Xuebao(Acta Prataculturae Sinica),23(3):144-151(季杨,张新全,彭燕,梁小玉,黄琳凯,马啸,马迎梅,2014,干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响,草业学报,23(3):144-151)
Jia X.J.,Dong L.H.,Ding C.B.,Li X.,and Yuan M.,2013,Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var.medio-pictum leaf,Caoye Xuebao(Acta Prataculturae Sinica),22(5):248-255(贾学静,董立花,丁春邦,李旭,袁明,2013,干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响,草业学报,22(5):248-255)
Jin Y.Q.,Li D.L.,Chen X.X.,and Zhang L.J.,2012,Physiological response of Sapium sebiferum seedlings from different provenances to drought stress,Xibei Zhiwu Xuebao(Acta Bot.Boreal.-Occident.Sin.),32(7):1395-1402(金雅琴,李冬林,陈小霞,张丽娟,2012,不同种源乌桕幼苗对干旱胁迫的生理响应,西北植物学报,32(7):1395-1402)
Li L.,Liu Y.M.,Wang M.,Wu N.,Liu Z.Y.,and Weng M.,2014,Physiological response mechanism of three kinds of Acer rubrum L.under continuous high temperature and drought stress,Shengtai Xuebao(Acta Ecologica Sinica),34(22):6471-6480(李力,刘玉民,王敏,吴念,刘正艳,翁敏,2014,3种北美红枫对持续高温干旱胁迫的生理响应机制,生态学报,34(22):6471-6480)
Meng M.,Huang X.F.,Li L.,Luo Y.C.,and Wang W.S.,2017,Effects of arsenic stress on activities of antioxidant enzymes of Eucalyptus,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(12):5289-5295(蒙敏,黄雪芬,李磊,罗宇晨,王维生,2017,砷胁迫对桉树抗氧化酶活性的影响,基因组学与应用生物学,36(12):5289-5295)
Pan X.,Qiu Q.,Li J.Y.,Wang J.H.,He Q.,Su Y.,Ma J.W.,and Du K.,2014,Physiological indexes of six plant species from the Tibetan Plateau under drought stress,Shengtai Xuebao(Acta Ecologica Sinica),34(13):3558-3567(潘昕,邱权,李吉跃,王军辉,何茜,苏艳,马建伟,杜坤,2014,干旱胁迫对青藏高原6种植物生理指标的影响,生态学报,34(13):3558-3567)
Qiu Z.J.,Li Y.,and Chong P.F.,2011,Effects of PEG stress on physiological characteristics of Calligonum mongolicum in different geographical provenance,Caoye Xuebao(Acta Prataculturae Sinica),20(3):108-114(邱真静,李毅,种培芳,2011,PEG胁迫对不同地理种源沙拐枣生理特性的影响,草业学报,20(3):108-114)
Ren L.,Zhao X.L.,Xu J.,Zhang H.Y.,Guo Y.H.,Guo F.L.,Zhang C.L.,and LüJ.H.,2015,Varied morphological and physiological responses to drought stress among four tea Chrysanthemum cultivars,Shengtai Xuebao(Acta Ecologica Sinica),35(15):5131-5139(任磊,赵夏陆,许婧,张宏毅,郭彦宏,郭福龙,张春来,吕晋慧,2015,4种茶菊对干旱胁迫的形态和生理响应,生态学报,35(15):5131-5139)
Shi Z.J.,Hu Z.S.,and Li R.S.,2002,Water stress and active oxygen metabolism,Guizhou Daxue Xuebao(Journal of Guizhou University),21(2):140-145(时忠杰,胡哲森,李荣生,2002,水分胁迫与活性氧代谢,贵州大学学报,21(2):140-145)
Tao J.J.,Xie J.,Wang Y.R.,Gao L.,Xiong Y.F.,and Sheng D.Y.,2015,Effect of different pre-cooling methods on quality of cutting greengrocery under low temperature storage,Shipin Gongye Keji(Science and Technology of Food Industry),36(18):337-341(陶佳佳,谢晶,王颖荣,高磊,熊月丰,盛东毅,2015,不同预冷方式对贮藏过程切割青菜品质的影响,食品工业科技,36(18):337-341)
Wang M.,Sha W.,Zhang M.J.,Zhu W.W.,and Xin Y.M.,2015,Effects of high temperature stress on the physiological and biochemical characteristics in Grimmia pilifera,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1290-1295(王曼,沙伟,张梅娟,朱巍巍,信雨萌,2015,高温胁迫对毛尖紫萼藓生理生化特性的影响,基因组学与应用生物学,34(6):1290-1295)
Wang W.,Jiang W.,Zhang J.L.,Miao L.,Zhao T.J.,Gai J.Y.,and Li Y.,2015,Selection of drought-tolerant soybean and evaluation of the drought-tolerance indices,Dadou Kexue(Soybean Science),34(5):808-818(王伟,姜伟,张金龙,苗龙,赵团结,盖钧镒,李艳,2015,大豆种质耐旱性鉴定及耐旱性指标筛选,大豆科学,34(5):808-818)
Wu M.,Zhang W.H.,Zhou J.Y.,Ma C.,and Han W.J.,2014,Effects of drought stress on growth,physiological and biochemical parameters in fine roots of Quercus variabilis Bl.seedlings,Shengtai Xuebao(Acta Ecologica Sinica),34(15):4223-4233(吴敏,张文辉,周建云,马闯,韩文娟,2014,干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响,生态学报,34(15):4223-4233)
Xia Q.,He B.H.,Liu Y.M.,and Xu J.,2010,Effects of high temperature stress on the morphological and physiological characteristics in Scaevola albida cutting seedlings,Shengtai Xuebao(Acta Ecologica Sinica),30(19):5217-5224(夏钦,何丙辉,刘玉民,徐健,2010,高温胁迫对粉带扦插苗形态和生理特征的影响,生态学报,30(19):5217-5224)
Xie X.Y.,Ma Z.L.,Bai P.,and Liu X.J.,2014,The morphological and physiological responses of hot pepper(Capsicum annuum L.)to drought stress with different intensity during blossom and fruit period,Shengtai Xuebao(Acta Ecologica Sinica),34(13):3797-3805(谢小玉,马仲炼,白鹏,刘晓健,2014,辣椒开花结果期对干旱胁迫的形态与生理响应,生态学报,34(13):3797-3805)
Xu C.,Zhang P.,Xu K.Z.,Li D.Y.,Ji P.,Feng Y.D.,and Zhao L.P.,2013,Effects of drought stress on leaf photosynthesis and some physiological traits in different soybean cultivars,Zhongguo Youliao Zuowu Xuebao(Chinese Journal of Oil Crop Sciences),35(6):674-679(徐晨,张鹏,徐克章,李大勇,季平,冯引娣,赵兰坡,2013,干旱胁迫对不同大豆品种叶片光合及生理特性的影响,中国油料作物学报,35(6):674-679)
Zhang C.,Chen H.S.,Nie Y.P.,Zhang W.,Feng T.,and Wang K.L.,2013,Dynamics of soil profile water content in peak-cluster depression areas in karst region,Zhongguo Shengtai Xuebao(Chinese Journal of Eco-Agriculture),21(10):1225-1232(张川,陈洪松,聂云鹏,张伟,冯腾,王克林,2013,喀斯特地区洼地面土壤含水率的动态变化规律,中国生态学报,21(10):1225-1232)
Zhang C.M.,Shi S.L.,and Wu F.,2018,Effects of drought stress on root and physiological responses of different drought-tolerant alfalfa varieties,Zhongguo Nongye Kexue(Scientia A-gricultura Sinica),51(5):868-882(张翠梅,师尚礼,吴芳,2018,干旱胁迫对不同抗旱性苜蓿品种根系生长及生理特性影响,中国农业科学,51(5):868-882)
Zhang Y.F.,and Yin B.,2009,Effects of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage,Caoye Xuebao(Acta Prataculturae Sinica),18(1):46-50(张永峰,殷波,2009,混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响,草业学报,18(1):46-50)
Chen J.,Gao G.Z.,Zhao L.,Xu L.S.,Zhang X.T.,and Xue W.,2015,Effects of wheat seed soaking with chitosan on protective enzymetic activities in germination stage under drought stress,Jiyinzuxue Yu Yingyong Sh engwuxue(Genomics and Applied Biology),35(10):2251-2254(陈军,高贵珍,赵亮,徐礼生,张兴桃,薛文,2015,干旱胁迫下壳聚糖浸种对小麦萌发期保护酶活性的影响,基因组学与应用生物学,35(10):2251-2254)
Chong P.F.,Li H.Y.,and Li Y.,2015,Physiological responses of seedling roots of the desert plant R eaumuria soongorica to drought stress,Caoye Xuebao(Acta Prataculturae Sinica),24(1):72-80(种培芳,李航毅,李毅,2015,荒漠植物红砂根系对干旱胁迫的生理响应,草业学报,24(1):72-80)
Guo Y.P.,Mi F.G.,Yan L.J.,Ren Y.X.,LüS.J.,and Fu B.Z.,2014,Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties,Caoye Xuebao(Acta Prataculturae Sinica),23(4):220-228(郭郁频,米富贵,闫利军,任永霞,吕世杰,伏兵哲,2014,不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价,草业学报,23(4):220-228)
Han R.H.,Tian H.,Gao G.J.,and Lu X.S.,2008,Change of water metabolism and two osmotic adjustment substances in the leaves of alfalfa under drought stress.,Huabei Nongxuebao(Acta Aoriculturae Boreali-sinica),23(4):140-144(韩瑞宏,田华,高桂娟,卢欣石,2008,干旱胁迫下紫花苜蓿叶片水分代谢与两种渗透调节物质的变化,华北农学报,23(4):140-144)
Ji Y.,Zhang X.Q.,Peng Y.,Liang X.Y.,Huang L.K.,Ma X.,and Ma Y.M.,2014,Effects of drought stress on lipid peroxidation,osmotic adjustment and activities of protective enzymes in the roots and leaves of orchardgrass,Caoye Xuebao(Acta Prataculturae Sinica),23(3):144-151(季杨,张新全,彭燕,梁小玉,黄琳凯,马啸,马迎梅,2014,干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响,草业学报,23(3):144-151)
Jia X.J.,Dong L.H.,Ding C.B.,Li X.,and Yuan M.,2013,Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var.medio-pictum leaf,Caoye Xuebao(Acta Prataculturae Sinica),22(5):248-255(贾学静,董立花,丁春邦,李旭,袁明,2013,干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响,草业学报,22(5):248-255)
Jin Y.Q.,Li D.L.,Chen X.X.,and Zhang L.J.,2012,Physiological response of Sapium sebiferum seedlings from different provenances to drought stress,Xibei Zhiwu Xuebao(Acta Bot.Boreal.-Occident.Sin.),32(7):1395-1402(金雅琴,李冬林,陈小霞,张丽娟,2012,不同种源乌桕幼苗对干旱胁迫的生理响应,西北植物学报,32(7):1395-1402)
Li L.,Liu Y.M.,Wang M.,Wu N.,Liu Z.Y.,and Weng M.,2014,Physiological response mechanism of three kinds of Acer rubrum L.under continuous high temperature and drought stress,Shengtai Xuebao(Acta Ecologica Sinica),34(22):6471-6480(李力,刘玉民,王敏,吴念,刘正艳,翁敏,2014,3种北美红枫对持续高温干旱胁迫的生理响应机制,生态学报,34(22):6471-6480)
Meng M.,Huang X.F.,Li L.,Luo Y.C.,and Wang W.S.,2017,Effects of arsenic stress on activities of antioxidant enzymes of Eucalyptus,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(12):5289-5295(蒙敏,黄雪芬,李磊,罗宇晨,王维生,2017,砷胁迫对桉树抗氧化酶活性的影响,基因组学与应用生物学,36(12):5289-5295)
Pan X.,Qiu Q.,Li J.Y.,Wang J.H.,He Q.,Su Y.,Ma J.W.,and Du K.,2014,Physiological indexes of six plant species from the Tibetan Plateau under drought stress,Shengtai Xuebao(Acta Ecologica Sinica),34(13):3558-3567(潘昕,邱权,李吉跃,王军辉,何茜,苏艳,马建伟,杜坤,2014,干旱胁迫对青藏高原6种植物生理指标的影响,生态学报,34(13):3558-3567)
Qiu Z.J.,Li Y.,and Chong P.F.,2011,Effects of PEG stress on physiological characteristics of Calligonum mongolicum in different geographical provenance,Caoye Xuebao(Acta Prataculturae Sinica),20(3):108-114(邱真静,李毅,种培芳,2011,PEG胁迫对不同地理种源沙拐枣生理特性的影响,草业学报,20(3):108-114)
Ren L.,Zhao X.L.,Xu J.,Zhang H.Y.,Guo Y.H.,Guo F.L.,Zhang C.L.,and LüJ.H.,2015,Varied morphological and physiological responses to drought stress among four tea Chrysanthemum cultivars,Shengtai Xuebao(Acta Ecologica Sinica),35(15):5131-5139(任磊,赵夏陆,许婧,张宏毅,郭彦宏,郭福龙,张春来,吕晋慧,2015,4种茶菊对干旱胁迫的形态和生理响应,生态学报,35(15):5131-5139)
Shi Z.J.,Hu Z.S.,and Li R.S.,2002,Water stress and active oxygen metabolism,Guizhou Daxue Xuebao(Journal of Guizhou University),21(2):140-145(时忠杰,胡哲森,李荣生,2002,水分胁迫与活性氧代谢,贵州大学学报,21(2):140-145)
Tao J.J.,Xie J.,Wang Y.R.,Gao L.,Xiong Y.F.,and Sheng D.Y.,2015,Effect of different pre-cooling methods on quality of cutting greengrocery under low temperature storage,Shipin Gongye Keji(Science and Technology of Food Industry),36(18):337-341(陶佳佳,谢晶,王颖荣,高磊,熊月丰,盛东毅,2015,不同预冷方式对贮藏过程切割青菜品质的影响,食品工业科技,36(18):337-341)
Wang M.,Sha W.,Zhang M.J.,Zhu W.W.,and Xin Y.M.,2015,Effects of high temperature stress on the physiological and biochemical characteristics in Grimmia pilifera,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1290-1295(王曼,沙伟,张梅娟,朱巍巍,信雨萌,2015,高温胁迫对毛尖紫萼藓生理生化特性的影响,基因组学与应用生物学,34(6):1290-1295)
Wang W.,Jiang W.,Zhang J.L.,Miao L.,Zhao T.J.,Gai J.Y.,and Li Y.,2015,Selection of drought-tolerant soybean and evaluation of the drought-tolerance indices,Dadou Kexue(Soybean Science),34(5):808-818(王伟,姜伟,张金龙,苗龙,赵团结,盖钧镒,李艳,2015,大豆种质耐旱性鉴定及耐旱性指标筛选,大豆科学,34(5):808-818)
Wu M.,Zhang W.H.,Zhou J.Y.,Ma C.,and Han W.J.,2014,Effects of drought stress on growth,physiological and biochemical parameters in fine roots of Quercus variabilis Bl.seedlings,Shengtai Xuebao(Acta Ecologica Sinica),34(15):4223-4233(吴敏,张文辉,周建云,马闯,韩文娟,2014,干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响,生态学报,34(15):4223-4233)
Xia Q.,He B.H.,Liu Y.M.,and Xu J.,2010,Effects of high temperature stress on the morphological and physiological characteristics in Scaevola albida cutting seedlings,Shengtai Xuebao(Acta Ecologica Sinica),30(19):5217-5224(夏钦,何丙辉,刘玉民,徐健,2010,高温胁迫对粉带扦插苗形态和生理特征的影响,生态学报,30(19):5217-5224)
Xie X.Y.,Ma Z.L.,Bai P.,and Liu X.J.,2014,The morphological and physiological responses of hot pepper(Capsicum annuum L.)to drought stress with different intensity during blossom and fruit period,Shengtai Xuebao(Acta Ecologica Sinica),34(13):3797-3805(谢小玉,马仲炼,白鹏,刘晓健,2014,辣椒开花结果期对干旱胁迫的形态与生理响应,生态学报,34(13):3797-3805)
Xu C.,Zhang P.,Xu K.Z.,Li D.Y.,Ji P.,Feng Y.D.,and Zhao L.P.,2013,Effects of drought stress on leaf photosynthesis and some physiological traits in different soybean cultivars,Zhongguo Youliao Zuowu Xuebao(Chinese Journal of Oil Crop Sciences),35(6):674-679(徐晨,张鹏,徐克章,李大勇,季平,冯引娣,赵兰坡,2013,干旱胁迫对不同大豆品种叶片光合及生理特性的影响,中国油料作物学报,35(6):674-679)
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