摘要
本试验旨在研究饲料精氨酸水平对黄颡鱼(Pelteobagrus fulvidraco)幼鱼生长性能、消化吸收相关指标、免疫功能和抗氧化能力的影响。选取初始体均重为(1.13±0.02) g的黄颡鱼幼鱼700尾,随机分为5组(每组4个重复,每个重复35尾鱼),分别投喂精氨酸水平为2.44%、2.64%、2.81%、3.01%和3.23%的等氮等脂试验饲料(蛋白质水平为42%,脂肪水平为9%)。试验期56 d。结果显示:1)与3.23%组相比,2.64%组和2.81%组黄颡鱼幼鱼的增重率(WGR)显著升高(P<0.05),2.64%组、2.81%组和3.01%组黄颡鱼幼鱼的饲料系数(FCR)显著降低(P<0.05)。2)黄颡鱼幼鱼的胃蛋白酶和淀粉酶活性在3. 01%组达到最高,显著高于2. 44%组(P <0.05); 2.81%组和3.01%组黄颡鱼幼鱼的胃脂肪酶活性显著高于其他各组(P <0. 05); 2. 64%、2.81%组、3. 23%组黄颡鱼幼鱼的肝脏淀粉酶活性显著高于2. 44%组(P <0. 05)。2. 64%组、2.81%组、3.01%组和3.23%组黄颡鱼幼鱼的肠道碱性磷酸酶(AKP)活性显著高于2.44%组(P<0.05); 2.64%组、2.81%组黄颡鱼幼鱼的肠道γ-谷氨酰转移酶(γ-GT)活性显著高于2.44%组、3.01%组和3.23%组(P<0.05)。3) 2.81%组和3.01%组黄颡鱼幼鱼的血清一氧化氮(NO)含量显著高于2.44%组(P<0.05),2.64%组、2.81%组、3.01%组和3.23%组黄颡鱼幼鱼的血清白细胞介素-1β(IL-1β)含量均显著低于2.44%组(P<0.05),2.81%组黄颡鱼幼鱼的血清白细胞介素-6(IL-6)含量显著低于2.44%组和2.64%组(P<0.05)。4) 2.64%组黄颡鱼幼鱼血清谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性显著高于2.44%组(P<0.05),3.01%组黄颡鱼幼鱼血清丙二醛(MDA)含量显著低于2.64%组(P<0.05)。由此得出,饲料中适宜水平的精氨酸可提高黄颡鱼幼鱼的WGR,降低FCR,增强消化吸收能力、免疫功能和抗氧化能力。以黄颡鱼幼鱼的WGR和血清NO含量为评价指标,经二次回归模型分析得出黄颡鱼幼鱼对饲料中精氨酸的需求量分别为2.74%(占饲料蛋白质的6.45%)和2.94%(占饲料蛋白质的6.92%)。
This experiment was conducted to investigate the effects of dietary arginine level on growth performance,digestive and absorptive related indexes,immune function and antioxidant ability of juvenile yellowcatfish( Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic diets( 42% protein and 9% lipid) were formulated to contain graded levels of arginine( 2.44%,2.64%,2.81%,3.01% and 3.23% of diet,respectively). Seven hundred juvenile yellowcatfish with an initial average body weight of( 1. 13 ± 0. 02) g were randomly divided into 5 groups with 4 replicates of 35 fish each and each group was fed one of the diets. The experiment lasted for 56 days. The results showed as follows: 1) the weight gain rate( WGR) of juvenile yellowcatfish in 2.64% and 2. 81% groups was significantly higher than that in 3. 23% group( P < 0. 05). The feed conversion ratio( FCR) of juvenile yellowcatfish in 2.64%,2.81% and 3.01% groups was significantly decreased when compared with 3. 23% group( P < 0. 05). 2) The activities of stomach protease and amylase in3.01% group were significantly higher than those in 2.44% group( P < 0.05),and the activity of stomach lipase in 2.81% and 3.01% groups was significantly higher than that in other groups( P<0.05). The activity of liver amylase in 2.64%,2. 81% and 3. 23% groups was significantly higher than that in 2. 44% group( P <0.05). The activity of intestinal alkaline phosphatase( AKP) in 2. 64%,2. 81%,3. 01% and 3. 23% groups was significantly higher than that in 2.44% group( P < 0.05). The activity of intestinal γ-glutamyl transferase(γ-GT) in 2.64% and 2.81% groups was significantly higher than that in 2.44%,3.01% and 3.23% groups(P<0.05). 3) Serum nitric oxide(NO) content in 2.81% and 3.01% groups was significantly higher than that in 2.44% group( P < 0. 05). The content of serum interleukin-1β( IL-1β) in 2. 64%,2. 81%,3. 01% and3.23% groups was significantly lower than that in 2.44% group( P<0.05),and the content of serum interleukin-6( IL-6) in 2.81% group was significantly lower than that in 2. 44% and 2. 64% groups( P < 0. 05). 4)The serum glutathion peroxidase( GSH-Px) and catalase( CAT) activities in 2.64% group were significantly higher than those in 2.44% group( P < 0. 05). The serum malondialdehyde( MDA) content in 3. 01% group was significantly lower than that in 2.64% group( P<0.05). In conclusion,optimal dietary arginine level can increase the WGR,decrease FCR,and enhance the digestion and absorption capacity,immune function and antioxidant ability of juvenile yellowcatfish. A quadratic regression analysis on WGR or serum NO content against dietary arginine level indicate that dietary arginine requirement of juvenile yellowcatfish is estimated to be 2.74% or 2.94% of diet( 6.45% or 6.92 of dietary protein).
引文
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