人参属植物达玛烷合成酶基因的生物信息学分析
|
摘要
对人参属植物皂苷生物合成途径中已知的达玛烷合成酶基因及其蛋白序列进行分子层面的比较分析,揭示该基因在不同物种间的进化关系。从Genbank中找到目前已公布的12种人参属植物达玛烷合成酶基因的序列信息,利用生物信息学方法对这些序列进行开放阅框预测、核酸及氨基酸序列的比对、序列理化性质分析、蛋白结构域预测以及系统发育树的构建。结果表明不同物种间的达玛烷合成酶基因在核苷酸和氨基酸平均相似性分别为99. 13%和98. 85%。通过系统发育树分析发现这12种不同来源的达玛烷合成酶基因按照聚类关系主要分为两个大分支,其中大部分之间有着较高的亲缘关系。通过生物信息学方法对人参属植物达玛烷合成酶基因进行分析,为深入阐明人参属植物中达玛烷皂苷途径的在不同物种间的进化关系,为探索人参皂苷合成的分子机制提供理论上的依据。
To investigate dammarenediol synthase and its coding gene in dammarane saponin biosynthesis pathway of Panax plants by bioinformatics analysis. Twelve dammarenediol synthase genes of Panax plants published on Genbank were analyzed in this study. Dammarenediol synthase cDNA sequence and its amino acid sequence,ORF and evolutional relationship were respectively analyzed using bioinformatics methods. The different species of dammarenediol synthase gene in nucleotides and amino acids of average similarity and structural analysis showed that 12 kinds of dammarenediol synthase gene encoding in ginseng genus were stable. By phylogenetic tree analysis,all these dammarenediol synthase could be divided into 4 main evolutionary directions,most of which have high affinities. By analyzing the bioinformatical characteristics of Panax dammarenediol synthase and its coding genes,the present study could provide theoretical basis and guidance for the future dammarenediol synthase gene and dammarane saponin pathway in genetic manipulation.
To investigate dammarenediol synthase and its coding gene in dammarane saponin biosynthesis pathway of Panax plants by bioinformatics analysis. Twelve dammarenediol synthase genes of Panax plants published on Genbank were analyzed in this study. Dammarenediol synthase cDNA sequence and its amino acid sequence,ORF and evolutional relationship were respectively analyzed using bioinformatics methods. The different species of dammarenediol synthase gene in nucleotides and amino acids of average similarity and structural analysis showed that 12 kinds of dammarenediol synthase gene encoding in ginseng genus were stable. By phylogenetic tree analysis,all these dammarenediol synthase could be divided into 4 main evolutionary directions,most of which have high affinities. By analyzing the bioinformatical characteristics of Panax dammarenediol synthase and its coding genes,the present study could provide theoretical basis and guidance for the future dammarenediol synthase gene and dammarane saponin pathway in genetic manipulation.
引文
[1]金士琨.人参2,3-氧化角鲨烯代谢途径中关键酶的表达调控研究[D].长春:吉林大学,2014.
[2]Kimura Y,Sumiyoshi M,Kawahira K,et al.Effects of ginseng sapoins isolated from Red Ginseng roots on burn wound healing in mice[J].Br J Pharmacol.,2006,148:860-70.
[3]Yokozawa T,Satoh A,Cho E J.GinsengsideRd attenuates oxidative damage related to aging in senescence-accelerated mice[J].J Pharm Pharmacol,2004,56:107-13.
[4]Xie X,Eberding A,Madera C,et al.Goldenberg L,et al.Rh2 synergistically enhances paclitaxel or mitoxantrone in prostate cancer models[J].J Urol,2006,175:1926-31
[5]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-1206.
[6]杨金玲,高丽丽,朱平.人参皂苷生物合成研究进展[J].药学学报,2013,48(2):170-178.
[7]赵灿,郭丽娜,彭玉帅,等.三七总皂苷生物合成的关键酶及其调控研究进展[J].中草药,2015,46(19):2954-2965.
[8]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-166
[9]张艳敬,候志芳,梁韶,等.人参属药用植物三萜皂苷合成途径关键酶的研究进展[J].特产研究,2016,38(2):53-57.
[10]许晓双,张福生,秦雪梅.三萜皂苷生物合成途径及关键酶的研究进展[J].世界科学技术-中医药现代化,2014,16(11):2440-2448.
[11]杨玲,范伟,孟珍贵,等.药用植物四环三萜皂苷生物合成及代谢研究进展[J].世界科学技术-中医药现代化,2018,20(06):1018-1025.
[2]Kimura Y,Sumiyoshi M,Kawahira K,et al.Effects of ginseng sapoins isolated from Red Ginseng roots on burn wound healing in mice[J].Br J Pharmacol.,2006,148:860-70.
[3]Yokozawa T,Satoh A,Cho E J.GinsengsideRd attenuates oxidative damage related to aging in senescence-accelerated mice[J].J Pharm Pharmacol,2004,56:107-13.
[4]Xie X,Eberding A,Madera C,et al.Goldenberg L,et al.Rh2 synergistically enhances paclitaxel or mitoxantrone in prostate cancer models[J].J Urol,2006,175:1926-31
[5]王海南.人参皂苷药理研究进展[J].中国临床药理学与治疗学,2006,11(11):1201-1206.
[6]杨金玲,高丽丽,朱平.人参皂苷生物合成研究进展[J].药学学报,2013,48(2):170-178.
[7]赵灿,郭丽娜,彭玉帅,等.三七总皂苷生物合成的关键酶及其调控研究进展[J].中草药,2015,46(19):2954-2965.
[8]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-166
[9]张艳敬,候志芳,梁韶,等.人参属药用植物三萜皂苷合成途径关键酶的研究进展[J].特产研究,2016,38(2):53-57.
[10]许晓双,张福生,秦雪梅.三萜皂苷生物合成途径及关键酶的研究进展[J].世界科学技术-中医药现代化,2014,16(11):2440-2448.
[11]杨玲,范伟,孟珍贵,等.药用植物四环三萜皂苷生物合成及代谢研究进展[J].世界科学技术-中医药现代化,2018,20(06):1018-1025.