凉粉草含药血清与抗菌药联用对携带fosA3耐药基因的产CTX-M型ESBLs大肠埃希菌的抑菌效果
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摘要
为了探讨凉粉草含药血清与抗菌药联合使用对连续传代的携带fos A3耐药基因的产CTX-M型ESBLs大肠埃希菌的体外抑菌活性,应用二倍微量稀释法测定各抗菌药及凉粉草含药血清对细菌的最小抑菌浓度(MIC)值,以凉粉草含药血清的亚抑菌浓度(1/2 MIC)与抗菌药联合使用对大肠埃希菌进行传代。结果发现,凉粉草含药血清联合抗菌药对大肠埃希菌有不同程度的抑菌活性,与凉粉草联合使用可显著增强氨基糖苷类、生物碱类、多磷类、利氟霉素类、磺胺类抗菌药对耐药菌的抑制作用。
The aim of the present study was to investigate the in vitro antibacterial activity of Mesona chinensis Benth.-containing serum in combination with antimicrobial agents on fos A3 gene-carrying and CTX-M-producing Escherichia coli(E. coli). Twofold broth micro-dilution method was used to determine the minimum inhibitory concentration(MIC) of the selected antimicrobial agents and Mesona chinensis Benth.-containing serum against the E. coli strain separately; furthermore, the Mesona chinensis Benth.-containing serum of subinhibitory concentration(1/2 MIC) and antimicrobial agents were co-incubated with the E. coli strain to observe the co-inhibitory effect. The results showed that the combined application of Mesona chinensis Benth.-containing serum and antimicrobial agents had antibacterial activity against the fos A3 gene-carrying and CTX-M-producing E.coli strain, and the inhibitory effects of the selected antibacterial agents including aminoglycosides, alkaloids, polyphosphates,leflunomycin and sulfonamides against the drug resistant E. coli were strengthened by Mesona chinensis Benth..
The aim of the present study was to investigate the in vitro antibacterial activity of Mesona chinensis Benth.-containing serum in combination with antimicrobial agents on fos A3 gene-carrying and CTX-M-producing Escherichia coli(E. coli). Twofold broth micro-dilution method was used to determine the minimum inhibitory concentration(MIC) of the selected antimicrobial agents and Mesona chinensis Benth.-containing serum against the E. coli strain separately; furthermore, the Mesona chinensis Benth.-containing serum of subinhibitory concentration(1/2 MIC) and antimicrobial agents were co-incubated with the E. coli strain to observe the co-inhibitory effect. The results showed that the combined application of Mesona chinensis Benth.-containing serum and antimicrobial agents had antibacterial activity against the fos A3 gene-carrying and CTX-M-producing E.coli strain, and the inhibitory effects of the selected antibacterial agents including aminoglycosides, alkaloids, polyphosphates,leflunomycin and sulfonamides against the drug resistant E. coli were strengthened by Mesona chinensis Benth..
引文
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[16]HO P L,CHAN J,LO W U,et al.Plasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig[J].Vet Microbiol,2013,162(2/4):964-967.
[2]刘兰军,宋伟峰.凉粉草水煎液的高效液相色谱离子阱质谱分析研究[J].中国药物经济学,2013(3):61-63.
[3]卫生部批准DHA藻油、棉籽低聚糖等7种物品为新资源食品[J].中国食品学报,2010,10(2):88.
[4]李湧,余巍,马燕娟,等.凉粉草中天然有机酸化学成分的研究[J].食品研究与开发,2014,35(16):35-38,39.
[5]KNOTHE H,SHAH P,KRCMERY V,et al.Transferable resistance to cefotaxime,cefoxitin,cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens[J].Infection,1983,11(6):315-317.
[6]HOU J,YANG X,ZENG Z,et al.Detection of the plasmidencoded fosfomycin resistance gene fos A3 in Escherichia coli of food-animal origin[J].J Antimicrob Chemother,2013,68(4):766-770.
[7]杭永付,薛晓燕,方芸,等.中药抗菌和逆转耐药作用机制研究进展[J].中国药房,2011,22(47):4504-4507.
[8]徐海波,李彩君.中药血清药理学方法探讨[J].中国中医基础医学杂志,1999,5(8):13-16.
[9]CLSI.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals;approved standard-third edition[S].Wayne,PA:CLSI,2008.
[10]司红彬,宋剑武,王鹏霞,等.救必应中药血清与抗菌药联合对产ESBLs细菌抑菌效果的研究[J].中国畜牧兽医,2015,42(4):1009-1014.
[11]刘晓庚,陈梅梅.中国仙草的开发利用研究[J].食品研究与开发,2004,25(5):109-112.
[12]王妍,俞发.仙草抗乙型肝炎病毒的体外实验研究[J].辽宁中医药大学学报,2009,11(8):219-221.
[13]WIDYANINGSIH T D.Cytotoxic effect of water,ethanol and ethyl acetate extract of black cincau(Mesona Palustris BL)against hela cell culture[J].APCBEE Procedia,2012,2:110-114.
[14]BONNET R,SAMPAIO J L,CHANAL C,et al.A novel class A extended-spectrum beta-lactamase(BES-1)in Serratia marcescens isolated in Brazil[J].Antimicrob Agents Chemother,2000,44(11):3061-3068.
[15]BARLOW R S,PEMBERTON J M,DESMARCHELIER P M,et al.Isolation and characterization of integroncontaining bacteria without antibiotic selection[J].Antimicrob Agents Chemother,2004,48(3):838-842.
[16]HO P L,CHAN J,LO W U,et al.Plasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig[J].Vet Microbiol,2013,162(2/4):964-967.