微生物法快速测定食品中叶酸含量范围
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摘要
本实验旨在提出一种使用鼠李糖乳杆菌(Lactobacillus casei) ATCC 7469快速估算食品中叶酸含量范围的测定方法。利用鼠李糖乳杆菌生长过程中对叶酸需求的特异性,使用一次性离心管代替玻璃试管作为培养该菌的容器,将整个培养体系从10 m L缩小到1 m L,将培养时间从24 h缩短为8～12 h。在对得到的吸光度数据简单换算后,得到待测样品叶酸含量范围,并进行方法学考察,与国标法GB 5009.211-2014进行对比,判断其是否具有可行性。结果表明,本实验室条件下,该方法的检出限范围为0.4～0.8μg/100 g,线性范围为0.01～0.08 ng,回收率范围为82.1%～108.0%。本方法检出限范围可估算出叶酸含量下限范围,线性区间范围可估算出叶酸含量上限范围。相对于国标方法需要2～3 d时间测定,本方法只需1 d时间即可得到叶酸含量范围,其步骤简洁且节约试剂材料。
A method for rapid determination of folic acid content range in food by Lactobacillus casei ATCC 7469 was proposed in this paper.Since folic acid was the specific demand for the growth of Lactobacillus casei,the whole culture system was reduced from 10 m L to 1 m L in disposable centrifuge tubes instead of glass tubes and the incubation time was shortened from about 24 h to 8 ～ 12 h.After simple conversion of the absorbance data,the range of folic acid content of the sample was obtained,and the methodological investigation was carried out.The feasibility of the method was compared with the national standard method(GB 5009.211-2014).The results showed that,under the laboratory conditions,the detection limit of this method was 0.4～0.8 μg/100 g,the linear range 0.01～0.08 ng,and the recovery rate 82.1% ～108%.The detection limit range of this method could be used to estimate the lower limit range of folic acid content,and the linear range could be used to estimate the upper limit range of folic acid content.Compared with the national standard method,which took 2 ～ 3 d for the determination,this method only need 1 d,which had simpler steps,and could savereagent materials.
A method for rapid determination of folic acid content range in food by Lactobacillus casei ATCC 7469 was proposed in this paper.Since folic acid was the specific demand for the growth of Lactobacillus casei,the whole culture system was reduced from 10 m L to 1 m L in disposable centrifuge tubes instead of glass tubes and the incubation time was shortened from about 24 h to 8 ～ 12 h.After simple conversion of the absorbance data,the range of folic acid content of the sample was obtained,and the methodological investigation was carried out.The feasibility of the method was compared with the national standard method(GB 5009.211-2014).The results showed that,under the laboratory conditions,the detection limit of this method was 0.4～0.8 μg/100 g,the linear range 0.01～0.08 ng,and the recovery rate 82.1% ～108%.The detection limit range of this method could be used to estimate the lower limit range of folic acid content,and the linear range could be used to estimate the upper limit range of folic acid content.Compared with the national standard method,which took 2 ～ 3 d for the determination,this method only need 1 d,which had simpler steps,and could savereagent materials.
引文
[1]Crider K S,Bailey L B,Berry R J.Folic acid food fortification-Its history,effect,concerns,and future directions[J].Nutrients,2011,3(3):370-384.
[2]Wagner D.Vitamin analysis for the health and food sciences(second edition)[J].Trends in Food Science and Technology,2009,20(11):598-599.
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[8]孙春燕,赵慧春,欧阳津.流动注射化学发光法测定叶酸[J].分析试验室,2000,19(3):60-62.
[9]蔡建.超高效液相色谱-串联质谱法测定运动饮料中的叶酸[J].食品与发酵工业,2017(11):220-224.
[10]冀晔.超高效液相色谱-串联质谱法测定运动饮品中的叶酸[J].食品工业科技,2017(24):274-277.
[11]黎小兰,陈彩云,蔡伟江,等.液相色谱-质谱法测定保健食品乳清蛋白粉类中叶酸含量的前处理条件的优化[J].食品安全质量检测学报,2017,8(8):3085-3091.
[12]石岩,黄艳梅,熊婧,等.HPLC法测定保健食品中叶酸含量的不确定度评价研究[J].安徽农业科学,2014,30):10651-10653.
[13]And R J P,Flanagan V P.A quantitative Stable-Isotope LC-MS method for the determination of folic acid in fortified foods[J].Journal of Agricultural and Food Chemistry,2001,49(3):1282.
[14]邓小燕,李佳渝,谭克俊.基于碳量子点荧光分光光度法检测叶酸[J].分析化学,2014,42(4):542-526.
[15]郭健,李敏,孟妍,等.荧光分光光度法测定食品中叶酸的含量[J].中国公共卫生,2003,19(10):1259-1259.
[16]叶立,田义梅.紫外分光光度法测定叶酸片的含量[J].天津药学,2000(3):63-64.
[17]李江,严家俊,綦艳,等.酶联免疫法快速检测奶粉中的叶酸[J].中国乳品工业,2017,45(10):37-38.
[18]李贞.ELISA方法检测牛奶中叶酸含量[J].食品研究与开发,2016(19):137-141.
[19]蔡双福,张琴,黄耀雄.奶粉中微生物法测定强化叶酸的方法改进[J].中外食品工业,2015(2):35-37.
[20]李飞飞,于娟娟,高伟敏,等.新型维生素检测试剂盒测定奶粉中叶酸的含量[J].中国乳品工业,2016,44(10):45-47.
[21]马景宏,张旭,王为黎.微生物分析法测定保健食品中叶酸[J].中国微生态学杂志,2007,19(6):576-577.
[22]张慧,吴环,黄伟乾,等.微生物法快速检测婴幼儿配方乳粉中叶酸含量[J].食品研究与开发,2015(5):66-69.
[23]张旺,邹志飞,袁慕云,等.微孔板试剂盒法检测婴幼儿配方奶粉中叶酸质量分数[J].中国乳品工业,2013,41(2):59-60.
[24]石丹,贾云虹,包怡红,等.叶酸检测方法的研究现状及发展趋势[J].中国乳品工业,2009,37(3):42-45.
[25]李全霞,崔亚娟,陈兆天,等.利用冷冻保藏菌种测定食品中的水溶性维生素[J].食品与发酵工业,2016,42(1):59-63.
[2]Wagner D.Vitamin analysis for the health and food sciences(second edition)[J].Trends in Food Science and Technology,2009,20(11):598-599.
[3]Minh T N,Indrawati A,M.Hendrickx.Model studies on the stability of folic acid and 5-Methyltetrahydrofolic acid degradation during thermal treatment in combination with high hydrostatic pressure[J].Journal of Agricultural and Food Chemistry,2003,51(11):3352.
[4]Berry R J,Li Z,Erickson J D,et al.Correction:prevention of neural-tube defects with folic acid in China[J].New England Journal of Medicine,1999,341(24):1864.
[5]Hberg S E,London S J,Stigum H,et al.Folic acid supplements in pregnancy and early childhood respiratory health[J].Journal of Allergy and Clinical Immunology,2009,123(2):S18-S18.
[6]Liu S,West R,Randell E,et al.A comprehensive evaluation of food fortification with folic acid for the primary prevention of neural tube defects[J].BMC Pregnancy and Childbirth,2004,4(1):20-30.
[7]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中叶酸的测定GB 5009.211-2014[S].中国标准出版社.2014:2-6
[8]孙春燕,赵慧春,欧阳津.流动注射化学发光法测定叶酸[J].分析试验室,2000,19(3):60-62.
[9]蔡建.超高效液相色谱-串联质谱法测定运动饮料中的叶酸[J].食品与发酵工业,2017(11):220-224.
[10]冀晔.超高效液相色谱-串联质谱法测定运动饮品中的叶酸[J].食品工业科技,2017(24):274-277.
[11]黎小兰,陈彩云,蔡伟江,等.液相色谱-质谱法测定保健食品乳清蛋白粉类中叶酸含量的前处理条件的优化[J].食品安全质量检测学报,2017,8(8):3085-3091.
[12]石岩,黄艳梅,熊婧,等.HPLC法测定保健食品中叶酸含量的不确定度评价研究[J].安徽农业科学,2014,30):10651-10653.
[13]And R J P,Flanagan V P.A quantitative Stable-Isotope LC-MS method for the determination of folic acid in fortified foods[J].Journal of Agricultural and Food Chemistry,2001,49(3):1282.
[14]邓小燕,李佳渝,谭克俊.基于碳量子点荧光分光光度法检测叶酸[J].分析化学,2014,42(4):542-526.
[15]郭健,李敏,孟妍,等.荧光分光光度法测定食品中叶酸的含量[J].中国公共卫生,2003,19(10):1259-1259.
[16]叶立,田义梅.紫外分光光度法测定叶酸片的含量[J].天津药学,2000(3):63-64.
[17]李江,严家俊,綦艳,等.酶联免疫法快速检测奶粉中的叶酸[J].中国乳品工业,2017,45(10):37-38.
[18]李贞.ELISA方法检测牛奶中叶酸含量[J].食品研究与开发,2016(19):137-141.
[19]蔡双福,张琴,黄耀雄.奶粉中微生物法测定强化叶酸的方法改进[J].中外食品工业,2015(2):35-37.
[20]李飞飞,于娟娟,高伟敏,等.新型维生素检测试剂盒测定奶粉中叶酸的含量[J].中国乳品工业,2016,44(10):45-47.
[21]马景宏,张旭,王为黎.微生物分析法测定保健食品中叶酸[J].中国微生态学杂志,2007,19(6):576-577.
[22]张慧,吴环,黄伟乾,等.微生物法快速检测婴幼儿配方乳粉中叶酸含量[J].食品研究与开发,2015(5):66-69.
[23]张旺,邹志飞,袁慕云,等.微孔板试剂盒法检测婴幼儿配方奶粉中叶酸质量分数[J].中国乳品工业,2013,41(2):59-60.
[24]石丹,贾云虹,包怡红,等.叶酸检测方法的研究现状及发展趋势[J].中国乳品工业,2009,37(3):42-45.
[25]李全霞,崔亚娟,陈兆天,等.利用冷冻保藏菌种测定食品中的水溶性维生素[J].食品与发酵工业,2016,42(1):59-63.