多囊卵巢综合征患者外周血Chemerin水平的变化
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摘要
目的对比多囊卵巢综合征(PCOS)患者与正常育龄妇女外周血Chemerin水平的差异,并分析其影响因素。方法将2017年1月至2018年3月在深圳市宝安区妇幼保健院妇科、生殖健康科门诊就诊的42例PCOS患者作为PCOS组,同期在上述科室进行孕前体检的正常妇女43例作为对照组,采用酶联免疫吸附法检测两组研究对象外周血Chemerin水平,并采用线性回归分析代谢指标及性激素水平与研究对象Chemerin水平的相关性。结果 PCOS患者外周血Chemerin水平为(134.5±41.0) ng/mL,明显高于对照组的(94.8±52.1) ng/mL,差异有显著统计学意义(P<0.001);PCOS患者外周血Chemerin水平与体质量指数(BMI)及空腹血糖水平呈正相关(r值分别为0.034和0.004,P值均<0.05),但与促卵泡生成素、雌二醇等性激素水平无相关(r值分别为0.009和0.257,P值均>0.05);正常妇女的Chemerin水平与上述指标均无关系(P值均>0.05)。结论 PCOS患者外周血中Chemerin水平明显升高,且与代谢紊乱相关,将来可作为该病及其代谢状态的生物标志物。
Objective To compare the Chemerin levels in peripheral blood between patients with polycystic ovary syndrome(PCOS) and controls, and then to analyze the related risk factors. Methods Chemerin levels in peripheral blood of 42 patients with PCOS and 43 normal reproductive women were detected using enzyme-linked immunosorbent assay, and the associations of the Chemerin levels with metabolic indicators and sex hormone levels were further analyzed by linear regression. Results The peripheral blood chemerin level in PCOS patients was significantly higher than that of controls:(134.5±41.0) ng/mL vs(94.8±52.1) ng/mL, P<0.001. The Chemerin level in PCOS patients was correlated with the patient's body mass index(r=0.034, P<0.05) and fasting blood glucose level(r=0.004, P<0.05), but there was no correlation between the Chemerin level with sexual hormone levels like follicular stimulating hormone(r=0.009,P>0.05) and estradiol(r=0.257, P>0.05). In normal women, Chemerin levels were not correlated with either of the above indicators(all P>0.05). Conclusion Chemerin levels in peripheral blood of PCOS patients are significantly increased and correlated with metabolic disorders. It suggested that Chemerin level might serve as a biomarker for PCOS and its related metabolic status.
Objective To compare the Chemerin levels in peripheral blood between patients with polycystic ovary syndrome(PCOS) and controls, and then to analyze the related risk factors. Methods Chemerin levels in peripheral blood of 42 patients with PCOS and 43 normal reproductive women were detected using enzyme-linked immunosorbent assay, and the associations of the Chemerin levels with metabolic indicators and sex hormone levels were further analyzed by linear regression. Results The peripheral blood chemerin level in PCOS patients was significantly higher than that of controls:(134.5±41.0) ng/mL vs(94.8±52.1) ng/mL, P<0.001. The Chemerin level in PCOS patients was correlated with the patient's body mass index(r=0.034, P<0.05) and fasting blood glucose level(r=0.004, P<0.05), but there was no correlation between the Chemerin level with sexual hormone levels like follicular stimulating hormone(r=0.009,P>0.05) and estradiol(r=0.257, P>0.05). In normal women, Chemerin levels were not correlated with either of the above indicators(all P>0.05). Conclusion Chemerin levels in peripheral blood of PCOS patients are significantly increased and correlated with metabolic disorders. It suggested that Chemerin level might serve as a biomarker for PCOS and its related metabolic status.
引文
[1] DUNAIF A, THOMAS A. Current concepts in the polycystic ovary syndrome[J]. Annual Review of Medicine, 2001, 52(1):401-419.
[2] BUKULMEZ AAO. Leukocytes in ovarian function[J]. Human Reproduction Update, 2000, 6(1):1-15.
[3]张艳萍,翟艳红.血清TNF-α和IL-6与多囊卵巢综合征发病的相关性探讨[J].中国妇幼保健, 2012, 27(1):27-28.
[4] MCMILLAN J. Chemerin is a novel adipokine associated with obesity and metabolic syndrome[J]. Endocrinology, 2007, 148(10):4687-4694.
[5] PARK SE, PARK CY, SWEENEY G. Biomarkers of insulin sensitivity and insulin resistance:past, present and future[J]. Critical Reviews in Clinical Laboratory Sciences, 2015, 52(4):180-190.
[6] ZHANG R, LIU S, GUO B, et al. Chemerin induces insulin resistance in rat cardiomyocytes in part through the ERK1/2 signaling pathway[J]. Pharmacology, 2014, 94(5-6):259-264.
[7] GUVENC Y, VAR A, GOKER A, et al. Assessment of serum chemerin, vaspin and omentin-1 levels in patients with polycystic ovary syndrome[J]. Journal of International Medical Research, 2016, 44(4):796-805.
[8] GUZEL EC, CELIK C, ABALI R, et al. Omentin and chemerin and their association with obesity in women with polycystic ovary syndrome[J]. Gynecol Endocrinol, 2014, 30(6):419-422.
[9] YANG X, QUAN X, LAN Y, et al. Serum chemerin level in women with PCOS and its relation with the risk of spontaneous abortion[J].Gynecol Endocrinol. 2018, 34(10):864-867.
[10] GORALSKI KB, SINAL CJ. Elucidation of chemerin and chemokine-like receptor-1 function in adipocytes by adenoviral-mediated shRNA knockdown of gene expression[J]. Methods Enzymol, 2009,460(1):289-312.
[11] PARLEE SD, ERNST MC, MURUGANANDAN S, et al. Serum chemerin levels vary with time of day and are modified by obesity and tumor necrosis factor-alpha[J]. Endocrinology, 2010, 151(6):2590-2602.
[12] HART R, GREAVES DR. Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5[J]. Journal of Immunology, 2010, 185(6):3728-3739.
[13] ZHANG Z, WANG J, WANG H. Correlation of blood glucose, serum chemerin and insulin resistance with NAFLD in patients with type 2diabetes mellitus[J]. Exp Ther Med, 2018, 15(3):2936-2940.
[2] BUKULMEZ AAO. Leukocytes in ovarian function[J]. Human Reproduction Update, 2000, 6(1):1-15.
[3]张艳萍,翟艳红.血清TNF-α和IL-6与多囊卵巢综合征发病的相关性探讨[J].中国妇幼保健, 2012, 27(1):27-28.
[4] MCMILLAN J. Chemerin is a novel adipokine associated with obesity and metabolic syndrome[J]. Endocrinology, 2007, 148(10):4687-4694.
[5] PARK SE, PARK CY, SWEENEY G. Biomarkers of insulin sensitivity and insulin resistance:past, present and future[J]. Critical Reviews in Clinical Laboratory Sciences, 2015, 52(4):180-190.
[6] ZHANG R, LIU S, GUO B, et al. Chemerin induces insulin resistance in rat cardiomyocytes in part through the ERK1/2 signaling pathway[J]. Pharmacology, 2014, 94(5-6):259-264.
[7] GUVENC Y, VAR A, GOKER A, et al. Assessment of serum chemerin, vaspin and omentin-1 levels in patients with polycystic ovary syndrome[J]. Journal of International Medical Research, 2016, 44(4):796-805.
[8] GUZEL EC, CELIK C, ABALI R, et al. Omentin and chemerin and their association with obesity in women with polycystic ovary syndrome[J]. Gynecol Endocrinol, 2014, 30(6):419-422.
[9] YANG X, QUAN X, LAN Y, et al. Serum chemerin level in women with PCOS and its relation with the risk of spontaneous abortion[J].Gynecol Endocrinol. 2018, 34(10):864-867.
[10] GORALSKI KB, SINAL CJ. Elucidation of chemerin and chemokine-like receptor-1 function in adipocytes by adenoviral-mediated shRNA knockdown of gene expression[J]. Methods Enzymol, 2009,460(1):289-312.
[11] PARLEE SD, ERNST MC, MURUGANANDAN S, et al. Serum chemerin levels vary with time of day and are modified by obesity and tumor necrosis factor-alpha[J]. Endocrinology, 2010, 151(6):2590-2602.
[12] HART R, GREAVES DR. Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5[J]. Journal of Immunology, 2010, 185(6):3728-3739.
[13] ZHANG Z, WANG J, WANG H. Correlation of blood glucose, serum chemerin and insulin resistance with NAFLD in patients with type 2diabetes mellitus[J]. Exp Ther Med, 2018, 15(3):2936-2940.