摘要
采用纤维素酶法制备香蕉皮纳米纤维素。研究酶解时间、纤维素酶浓度、酶解温度3个因素对香蕉皮纳米纤维素产率的影响,通过响应曲面分析方法优化其酶解工艺,得到纤维素酶法制备香蕉皮纳米纤维素的最佳合适的工艺条件。结果表明:最佳条件为温度60℃,酶浓度200μ/mL,酶解时间180min,产率为53.08%。
Nano-cellulose in banana peel was prepared by cellulase. The effects of enzymolysis time,cellulase concentration and enzymolysis temperature on the yield of nano-cellulose in banana peel were studied. The process of enzymatic hydrolysis was optimized by the response surface analysis method,and the optimum process conditions for the production of nano-cellulose by cellulose enzyme were obtained. The results showed that the optimal conditions were :temperature was 60 ℃,enzyme concentration was 200 u/mL,enzymolysis time was 180 min,and the yield was 53.08%.
引文
[1]卢宏科,王琴,区子弁.膳食纤维的功能与应用[J].广东农业科学,2007(4):67-70.
[2]唐丽荣,黄彪,戴达松,等.纳米纤维素碱法制备及光谱性质[J].光谱学与光谱分析,2010,30(7):1876-1878.
[3]Favier V,Chanzy H,Cavaille J Y.Polymer nanocomposites reinforced by cellulose whiskers[J].Macromolecules,1995(28):6365-6367.
[4]Tang L R.Huang B,Ou W,et al.Manufacture of cellulose nanocrystals by cation exchange resin-catalyzed hy-drolysis of cellulose[J].Bioresour Technol,2011,102(23):10973-10977.
[5]Jean B,Dubreuil F,Heux L,et al.Structural details of cellulose nanocrystals/polyelectroly-tes multilayers probed by neutron reflectivity and AFM[J].Langmuir,2008,24(7):3452-3458.
[6]Dufresne A.Comparing the mechanical properties of high performances polymer nanocomposites from biological sources[J].Nanosci Nanotechnol,2006,6(2):322-330.
[7]Zimmermann T,Bordeanu N,Strub E.Properties of nanofibrillated cellulose from different raw materials and its reinforcement potential[J].Carbohydrate Polymers,2010,79(4):1086-1093.
[8]Wan Y Z,Huang Y,Yuan C D,et al.Biomimetic synthesis of hydroxyapatite/bacterial cellulose nanocomposites for biomedical applications[J].Materials Science and Engineering:C,2007(27):855-864.
[9]曲萍,高源,白露,等.聚乙二醇增容纳米纤维素/聚乳酸共混体系的研究[J].功能材料,2011,42(增刊):69-72.
[10]王希成.生物化学[M].北京:清华大学出版社,2001:71-76.
[11]孙克岩,张志胜,佟海菊,刘亚南,宋欣.响应面法优化木瓜蛋白酶和风味蛋白酶双酶酶解牡蛎工艺研究[J].食品科技,2011,36(6);14-15.