东乡野生稻低温发芽力QTL定位及超级稻耐冷改良
|
摘要
低温发芽力是限制直播稻种子成苗的主要因素之一。研究低温发芽力遗传及分子机制对选育适宜直播的优良水稻新品种意义重大。本研究利用东乡野生稻和超级稻沈农265通过多代回交和自交的方法构建的回交重组自交系群体,根据农艺性状和低温发芽力的综合表现,筛选了10份综合农艺性状良好且低温发芽力较强的株系,为超级稻沈农265低温发芽力的改良提供了基础材料。采用集团分离分析法(BSA),共检测到4个标记与低温发芽力连锁,分别是2号染色体RM324和RM166、5号染色体RM534和9号染色体RM257。进一步通过连锁分析,鉴定出15个低温发芽力相关QTL,分别位于第1、2、4、5、6、7和11号染色体,这些QTL的LOD值介于2.57~5.00之间,QTL贡献率介于11.48%~17.72%之间。其中位于2号染色体的qGP-2-1和qGP-2-4与BSA法检测到的连锁标记RM324和RM166一致,这两个位点可用于低温发芽力分子标记辅助选择。
The germination rate under relatively low temperature condition( GR-LT) is one of the main factors that restricts the development of direct-seeding rice. Understanding the genetic and molecular mechanism of GR-LT is significant interest in breeding for elite varieties in direct-seeding rice. In this study,140 backcross inbred lines( BILs),derived from a japonica super rice shennong265( SN265) as the recurrent parent and the Dongxiang wild rice( Oryza rufipogon Griff.) as the donor parent,were used for QTL mapping of GR-LT traits. According to the comprehensive performance under low temperature,10 lines with GR-LT and good agronomic traits were selected and served as valuable resources for improving GR-LT in super rice,SN265. The Bulked Segregate Analysis( BSA)revealed four genetic markers RM324 and RM166 on chromosome 2,RM534 on chromosome 5 and RM257 on chromosome 9,that linked to the GR-LT. A total of 15 QTLs were detected on chromosomes 1,2,4,5,6,7 and 11 by linkage map,which explained 11. 48% ~ 17. 72% of the phenotypic variance. Of that,qGP-2-1 and qGP-2-4 were associated with RM324 and RM166 that served as the selection markers for selecting HGR-LT in rice breeding.
The germination rate under relatively low temperature condition( GR-LT) is one of the main factors that restricts the development of direct-seeding rice. Understanding the genetic and molecular mechanism of GR-LT is significant interest in breeding for elite varieties in direct-seeding rice. In this study,140 backcross inbred lines( BILs),derived from a japonica super rice shennong265( SN265) as the recurrent parent and the Dongxiang wild rice( Oryza rufipogon Griff.) as the donor parent,were used for QTL mapping of GR-LT traits. According to the comprehensive performance under low temperature,10 lines with GR-LT and good agronomic traits were selected and served as valuable resources for improving GR-LT in super rice,SN265. The Bulked Segregate Analysis( BSA)revealed four genetic markers RM324 and RM166 on chromosome 2,RM534 on chromosome 5 and RM257 on chromosome 9,that linked to the GR-LT. A total of 15 QTLs were detected on chromosomes 1,2,4,5,6,7 and 11 by linkage map,which explained 11. 48% ~ 17. 72% of the phenotypic variance. Of that,qGP-2-1 and qGP-2-4 were associated with RM324 and RM166 that served as the selection markers for selecting HGR-LT in rice breeding.
引文
[1]吴文革,陈烨,钱银飞,王小军,吴一梅.水稻直播栽培的发展概况与研究进展.中国农业科技导报,2006,8(4):30-36
[2]赵步洪,戴正元,谢成林,王宝和,陆春泉,张洪熙.直播水稻的研究与应用进展及发展策略.江苏农业科学,2010(5):13-15
[3]Wan J,Tamura K,Sakai M,Imbe T.Linkage analysis of low temperature germinability in rice(Oryza sativa L.).Breeding Science,1999(S2):122
[4]Suh J P,Jeung J U,Lee J I,Choi Y H,Yea J D,Virk P S,Mackill D J,Jena K K.Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice(Oryza sativa L.).Theoretical and Applied Genetics,2010,120(5):985-995
[5]Miura K,Lin S Y,Yano M,Nagamine T.Mapping of quantitative trait loci controlling low temperature germinability in rice(Oryza sativa L.).Breeding Science,2001,51:293-299
[6]Teng S,Zeng D L,Qian Q.QTL analysis of rice low temperature germinability.Chinese Science Bulletin,2001,46(21):1800-1803
[7]纪素兰,江令,王益华,刘世家,刘喜,翟虎渠,吉村醇,万建民.水稻种子耐低温发芽力的QTL定位及上位性分析.作物学报,2008,34(4):551-556
[8]巩迎军,阮雯君,荀星,董彦君,林冬枝,叶胜海,张小明.水稻芽性状耐冷性的QTL分析.分子植物育种,2009,7(2):273-278
[9]Hou M Y,Wang C M,Jiang L,Wan J M.Inheritance and QTL mapping of low temperature genninability in rice(Oryza sativa L.).Acta Geneics Sinica,2004,31:701-706
[10]Han L Z,Zhang Y Y,Qiao Y L,Cao G L,Zhang S Y,Kim J H,Koh H J.Genetic and QTL analysis for low-temperature vigor of germination in rice.Acta Genetics Sinica,2006,33(11):998-1006
[11]陈亮,楼巧君,孙宗修,邢永忠,余新桥,罗利军.水稻低温发芽力的QTL定位.中国水稻科学,2006,20(2):159-164
[12]Jiang L,Liu S,Hou M,Tang J,Chen L,Zhai H,Wan J.Analysis of QTLs for seed low temperature germinability and anoxia germinability in rice(Oryza sativa L.).Field Crops Research,2006,98:68-75
[13]Fujino K,Sekiguchi H,Matsuda Y,Sugimoto K,Ono K,Yano M.Molecular identification of a major quantitative trait locus,q LTG3-1,controlling low-temperature germinability in rice.Proceedings of the National Academy of Sciences,2008,105(34):12623-12628
[14]Kenji F,Yasuyuki M.Genome-wide analysis of genes targeted by q LTG3-1 controlling low-temperature germinability in rice.Plant Molecular Biology,2009,72:137-152
[15]Kenji F,Hiroshi S.Origins of functional nucleotide polymorphisms in a major quantitative trait locus,q LTG3-1,controlling low-temperature germinability in rice.Plant Molecular Biology,2011,75(1):1-10
[16]向彦,贺浩华,刘宜柏,傅军如.东乡野生稻耐冷性研究进展.江西农业大学学报:自然科学版,2003,25(4):482-486
[17]陈大洲,肖叶青,赵社香,熊焕金,皮勇华,罗利军.东乡野生稻苗期和穗期的耐寒性研究.江西农业学报,1996(1):1-6
[18]夏瑞祥,肖宁,洪义欢,张超,苏琰,张小蒙,陈建民.东乡野生稻苗期耐冷性的QTL定位.中国农业科学,2010,43(3):443-451
[19]陈大洲,钟平安,肖叶青,黄英金,谢建坤.利用SSR标记定位东乡野生稻苗期耐冷性基因.江西农业大学学报:自然科学版,2002,24(6):753-756
[20]简水溶,万勇,罗向东,方军,储成才,谢建坤.东乡野生稻苗期耐冷性的遗传分析.植物学报,2011,46(1):21-27
[21]刘凤霞,孙传清,谭禄宾,李德军,付永彩,王象坤.江西东乡野生稻孕穗开花期耐冷基因定位.科学通报,2003,48(17):1864-1867
[22]张成良,姜伟,肖叶青,邬文昌,陈大洲,黄英金.东乡野生稻耐寒性研究现状与展望.种子,2006(10):44-47
[23]姜秀娟.利用东乡野生稻进行超级稻低温萌发力改良及QTL定位.沈阳:沈阳农业大学,2017
[24]金铭璐,杨春刚,余腾琼,郭桂珍,汤翠凤,张俊国,阿新祥,曹桂兰,徐福荣,刘宪虎,戴陆园,张三元,韩龙植.中国水稻微核心种质不同生育时期耐冷性鉴定及其相关分析.植物遗传资源学报,2009,10(4):540-546
[25]陈大洲,肖叶青,皮勇华,邬文昌,胡兰香,罗世友,谢金水.东乡野生稻耐冷性的遗传改良初步研究.江西农业大学学报:自然科学版,2003,25(1):8-11
[26]Tanksley S D,Nelson J C.Advanced backcross QTL analysis:A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines.Theoretical and Applied Genetics,1996,92:191-203
[27]Andaya V,Mackill D.QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica x indica cross.Theoretical and Applied Genetics,2003,106(6):1084-1090
[28]You J,Li Q,Yue B,Xue W Y.Identification of quantitative trait loci for ABA sensitivity at seed germination and seedling stages in rice.Acta Genetica Sinica,2006,33(6):532-541
[29]Shi J L,Cao Y P,Fan X R,Li M,Wang Y F,Feng M.A rice microsomal delta-12 fatty acid desaturase can enhance resistance to cold stress in yeast and Oryza sativa.Molecular Breeding,2012,29(3):743-757
[30]Han L Z,Qiao Y L,Zhang S Y,Zhang Y Y,Cao G L,Kim J H,Lee K S,Koh H J.Identification of quantitative trait loci for cold response of seedling vigor traits in rice.Acta Genetica Sinica,2007,34(3):239-246
[31]Suh J P,Ahn S N,Moon H P,Suh H S.QTL analysis of low temperature germinability in a Korean weedy rice(Oryza saliva L.).Rice Research Newsletter,1999,16:53-55
[32]Oh C S,Choi Y H,Lee S J,Yoon D B,Moon H P,Ahn S N.Mapping of quantitative trait loci for cold tolerance in weedy rice.Breeding Science,2004,54:373-380
[2]赵步洪,戴正元,谢成林,王宝和,陆春泉,张洪熙.直播水稻的研究与应用进展及发展策略.江苏农业科学,2010(5):13-15
[3]Wan J,Tamura K,Sakai M,Imbe T.Linkage analysis of low temperature germinability in rice(Oryza sativa L.).Breeding Science,1999(S2):122
[4]Suh J P,Jeung J U,Lee J I,Choi Y H,Yea J D,Virk P S,Mackill D J,Jena K K.Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice(Oryza sativa L.).Theoretical and Applied Genetics,2010,120(5):985-995
[5]Miura K,Lin S Y,Yano M,Nagamine T.Mapping of quantitative trait loci controlling low temperature germinability in rice(Oryza sativa L.).Breeding Science,2001,51:293-299
[6]Teng S,Zeng D L,Qian Q.QTL analysis of rice low temperature germinability.Chinese Science Bulletin,2001,46(21):1800-1803
[7]纪素兰,江令,王益华,刘世家,刘喜,翟虎渠,吉村醇,万建民.水稻种子耐低温发芽力的QTL定位及上位性分析.作物学报,2008,34(4):551-556
[8]巩迎军,阮雯君,荀星,董彦君,林冬枝,叶胜海,张小明.水稻芽性状耐冷性的QTL分析.分子植物育种,2009,7(2):273-278
[9]Hou M Y,Wang C M,Jiang L,Wan J M.Inheritance and QTL mapping of low temperature genninability in rice(Oryza sativa L.).Acta Geneics Sinica,2004,31:701-706
[10]Han L Z,Zhang Y Y,Qiao Y L,Cao G L,Zhang S Y,Kim J H,Koh H J.Genetic and QTL analysis for low-temperature vigor of germination in rice.Acta Genetics Sinica,2006,33(11):998-1006
[11]陈亮,楼巧君,孙宗修,邢永忠,余新桥,罗利军.水稻低温发芽力的QTL定位.中国水稻科学,2006,20(2):159-164
[12]Jiang L,Liu S,Hou M,Tang J,Chen L,Zhai H,Wan J.Analysis of QTLs for seed low temperature germinability and anoxia germinability in rice(Oryza sativa L.).Field Crops Research,2006,98:68-75
[13]Fujino K,Sekiguchi H,Matsuda Y,Sugimoto K,Ono K,Yano M.Molecular identification of a major quantitative trait locus,q LTG3-1,controlling low-temperature germinability in rice.Proceedings of the National Academy of Sciences,2008,105(34):12623-12628
[14]Kenji F,Yasuyuki M.Genome-wide analysis of genes targeted by q LTG3-1 controlling low-temperature germinability in rice.Plant Molecular Biology,2009,72:137-152
[15]Kenji F,Hiroshi S.Origins of functional nucleotide polymorphisms in a major quantitative trait locus,q LTG3-1,controlling low-temperature germinability in rice.Plant Molecular Biology,2011,75(1):1-10
[16]向彦,贺浩华,刘宜柏,傅军如.东乡野生稻耐冷性研究进展.江西农业大学学报:自然科学版,2003,25(4):482-486
[17]陈大洲,肖叶青,赵社香,熊焕金,皮勇华,罗利军.东乡野生稻苗期和穗期的耐寒性研究.江西农业学报,1996(1):1-6
[18]夏瑞祥,肖宁,洪义欢,张超,苏琰,张小蒙,陈建民.东乡野生稻苗期耐冷性的QTL定位.中国农业科学,2010,43(3):443-451
[19]陈大洲,钟平安,肖叶青,黄英金,谢建坤.利用SSR标记定位东乡野生稻苗期耐冷性基因.江西农业大学学报:自然科学版,2002,24(6):753-756
[20]简水溶,万勇,罗向东,方军,储成才,谢建坤.东乡野生稻苗期耐冷性的遗传分析.植物学报,2011,46(1):21-27
[21]刘凤霞,孙传清,谭禄宾,李德军,付永彩,王象坤.江西东乡野生稻孕穗开花期耐冷基因定位.科学通报,2003,48(17):1864-1867
[22]张成良,姜伟,肖叶青,邬文昌,陈大洲,黄英金.东乡野生稻耐寒性研究现状与展望.种子,2006(10):44-47
[23]姜秀娟.利用东乡野生稻进行超级稻低温萌发力改良及QTL定位.沈阳:沈阳农业大学,2017
[24]金铭璐,杨春刚,余腾琼,郭桂珍,汤翠凤,张俊国,阿新祥,曹桂兰,徐福荣,刘宪虎,戴陆园,张三元,韩龙植.中国水稻微核心种质不同生育时期耐冷性鉴定及其相关分析.植物遗传资源学报,2009,10(4):540-546
[25]陈大洲,肖叶青,皮勇华,邬文昌,胡兰香,罗世友,谢金水.东乡野生稻耐冷性的遗传改良初步研究.江西农业大学学报:自然科学版,2003,25(1):8-11
[26]Tanksley S D,Nelson J C.Advanced backcross QTL analysis:A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines.Theoretical and Applied Genetics,1996,92:191-203
[27]Andaya V,Mackill D.QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica x indica cross.Theoretical and Applied Genetics,2003,106(6):1084-1090
[28]You J,Li Q,Yue B,Xue W Y.Identification of quantitative trait loci for ABA sensitivity at seed germination and seedling stages in rice.Acta Genetica Sinica,2006,33(6):532-541
[29]Shi J L,Cao Y P,Fan X R,Li M,Wang Y F,Feng M.A rice microsomal delta-12 fatty acid desaturase can enhance resistance to cold stress in yeast and Oryza sativa.Molecular Breeding,2012,29(3):743-757
[30]Han L Z,Qiao Y L,Zhang S Y,Zhang Y Y,Cao G L,Kim J H,Lee K S,Koh H J.Identification of quantitative trait loci for cold response of seedling vigor traits in rice.Acta Genetica Sinica,2007,34(3):239-246
[31]Suh J P,Ahn S N,Moon H P,Suh H S.QTL analysis of low temperature germinability in a Korean weedy rice(Oryza saliva L.).Rice Research Newsletter,1999,16:53-55
[32]Oh C S,Choi Y H,Lee S J,Yoon D B,Moon H P,Ahn S N.Mapping of quantitative trait loci for cold tolerance in weedy rice.Breeding Science,2004,54:373-380