妊娠期糖尿病孕妇血清Th1/Th2比值测定及分析
摘要
目的测定妊娠期糖尿病(GDM)患者血清中Th1细胞分泌的细胞因子干扰素-γ(IFN-γ)及Th2细胞分泌的细胞因子白介素-4(IL-4)的水平,分析Th1/Th2比值与GDM的关系。方法选取2015年10月至2016年10月间在我院住院治疗的GDM孕妇30例为GDM组,同时随机选取同一时期内在我院住院的正常孕妇30例为对照组。两组对象于早晨抽取空腹静脉血测定糖化血红蛋白,采用ELISA方法测定两组对象血清中的IFN-γ和IL-4水平。比较两组对象的上述实验室指标及IFN-γ/IL-4比值。结果两组孕妇的年龄、孕周、糖化血红蛋白水平比较,均无显著性差异(P>0.05)。GDM组患者血清中IFN-γ水平显著低于对照组[(87.38±9.14)vs.(96.27±10.24)pg/ml](P<0.05),IL-4水平显著高于对照组[(49.09±1.44)vs.(42.38±1.28)pg/ml](P<0.05)。GDM组患者血清中IFN-γ/IL-4比值显著低于对照组[(1.78±0.11)vs.(2.27±1.07)](P<0.05)。结论 GDM患者体内存在着Th1/Th2失衡的现象,Th2更加优势表达。
引文
[1]Zeng W,Liu Z,Liu X,et al.Distinct transcriptional and alternative splicing signatures of decidual CD4+T cells in early human pregnancy[J].Front Immunol,2017,8:682.
[2]Wei B,Sheng Li C.Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease[J].J Int Med Res,2018,46:3890-3902.
[3]van Oosterhout AJ,Motta AC.Th1/Th2paradigm:not seeing the forest for the trees?[J].Eur Respir J,2005,25:591-593.
[4]Zhu M,Liang Z,Wang T,et al.Th1/Th2/Th17cells imbalance in patients with asthma with and without psychological symptoms[J].Allergy Asthma Proc,2016,37:148-156.
[5]张珺,杨菁.Th17/Treg免疫失衡与原因不明复发性流产关系的研究进展[J].生殖医学杂志,2015,24:960-964.
[6]Wu L,Luo LH,Zhang YX,et al.Alteration of Th17and Treg cells in patients with unexplained recurrent spontaneous abortion before and after lymphocyte immunization therapy[J].Reprod Biol Endocrinol,2014,12:74.
[7]Williams Z.Inducing tolerance to pregnancy[J].N Engl JMed,2012,367:1159-1161.
[8]Mj9sberg J,Berg G,Jenmalm MC,et al.FOXP3+regulatory Tcells and T helper 1,T helper 2,and T helper 17cells in human early pregnancy decidua[J].Biol Reprod,2010,82:698-705.
[9]Yuan J,Li J,Huang SY,et al.Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2cytokines in patients with unexplained recurrent spontaneous abortion[J].J Reprod Immunol,2015,110:81-88.
[10]Nakagawa K,Kuroda K,Sugiyama R,et al.After 12consecutive miscarriages,apatients received immunosuppressive treatment and delivered an intact baby[J].Reprod Med Biol,2017,16:297-301.
[11]Polese B,Gridelet V,Araklioti E,et al.The endocrine milieu and CD4 T-lymphocyte polarization during pregnancy[J].Front Endocrinol(Lausanne),2014,5:106.
[12]He M,Jiang M,Zhou Y,et al.Impaired Gal-9dysregulates the PBMC-induced Th1/Th2 imbalance in abortion-prone matings[J].J Immunol Res,2018,2018:9517842.
[13]Yessoufou A,Moutairou K.Maternal diabetes in pregnancy:early and long-term outcomes on the offspring and the concept of“metabolic memory”[J].Exp Diabetes Res,2011,2011:218598.
[14]Azizieh F,Dingle K,Raghupathy R,et al.Multivariate analysis of cytokine profiles in pregnancy complications[J].Am J Reprod Immunol,2018,79.doi:10.1111/aji.12818.
[15]Chen Y,Lv W,Jia J,et al.Evaluation of serum concentrations of tumor necrosis factor(TNF)-a,interleukin(IL)-2,IL-10,and nitric oxide(NO)during the estrous cycles,early pregnancy and abortion in goats[J].Anin Reprod Sci,2016,174:73-79.
[16]周素娴,夏宁,梁瑜桢,等.Graves眼病患者T细胞亚群的研究[J].中华内分泌代谢杂志,2006,22:126-127.
[17]Ategbo JM,Grissa O,Yessoufou A,et al.Modulation of adipokines and cytokines in gestational diabetes and macrosomia[J].J Clin Endocrinol Metab,2006,91:4137-4143.
[18]Sudharshana Murthy KA,Bhandiwada A,Chandan SL,et al.Evaluation of oxidative stress and proinflammatory cytokines in gestational diabetes mellitus and their correlation with pregnancy outcome[J].Indian J Endocrinol Metab,2018,22:79-84.
[19]Mrizak I,Grissa O,Henault B,et al.Placental infiltration of inflammatory markers in gestational diabetic women[J].Gen Physiol Biophys,2014,33:169-176.
[20]Viardot A,Grwey ST,Mackay E,et al.Potential antiinflammatory role of insulin via the preferential polarization of effector T cells toward a T helper2 phenotype[J].Endocrinology,2007,148:346-353.
[21]King MR,Ismail AS,Davis LS,et al.Oxidative stress promotes polarization of human T cell differentiation toward a T helper 2phenotype[J].J Immunol,2006,176:2765-2772.
[2]Wei B,Sheng Li C.Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease[J].J Int Med Res,2018,46:3890-3902.
[3]van Oosterhout AJ,Motta AC.Th1/Th2paradigm:not seeing the forest for the trees?[J].Eur Respir J,2005,25:591-593.
[4]Zhu M,Liang Z,Wang T,et al.Th1/Th2/Th17cells imbalance in patients with asthma with and without psychological symptoms[J].Allergy Asthma Proc,2016,37:148-156.
[5]张珺,杨菁.Th17/Treg免疫失衡与原因不明复发性流产关系的研究进展[J].生殖医学杂志,2015,24:960-964.
[6]Wu L,Luo LH,Zhang YX,et al.Alteration of Th17and Treg cells in patients with unexplained recurrent spontaneous abortion before and after lymphocyte immunization therapy[J].Reprod Biol Endocrinol,2014,12:74.
[7]Williams Z.Inducing tolerance to pregnancy[J].N Engl JMed,2012,367:1159-1161.
[8]Mj9sberg J,Berg G,Jenmalm MC,et al.FOXP3+regulatory Tcells and T helper 1,T helper 2,and T helper 17cells in human early pregnancy decidua[J].Biol Reprod,2010,82:698-705.
[9]Yuan J,Li J,Huang SY,et al.Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2cytokines in patients with unexplained recurrent spontaneous abortion[J].J Reprod Immunol,2015,110:81-88.
[10]Nakagawa K,Kuroda K,Sugiyama R,et al.After 12consecutive miscarriages,apatients received immunosuppressive treatment and delivered an intact baby[J].Reprod Med Biol,2017,16:297-301.
[11]Polese B,Gridelet V,Araklioti E,et al.The endocrine milieu and CD4 T-lymphocyte polarization during pregnancy[J].Front Endocrinol(Lausanne),2014,5:106.
[12]He M,Jiang M,Zhou Y,et al.Impaired Gal-9dysregulates the PBMC-induced Th1/Th2 imbalance in abortion-prone matings[J].J Immunol Res,2018,2018:9517842.
[13]Yessoufou A,Moutairou K.Maternal diabetes in pregnancy:early and long-term outcomes on the offspring and the concept of“metabolic memory”[J].Exp Diabetes Res,2011,2011:218598.
[14]Azizieh F,Dingle K,Raghupathy R,et al.Multivariate analysis of cytokine profiles in pregnancy complications[J].Am J Reprod Immunol,2018,79.doi:10.1111/aji.12818.
[15]Chen Y,Lv W,Jia J,et al.Evaluation of serum concentrations of tumor necrosis factor(TNF)-a,interleukin(IL)-2,IL-10,and nitric oxide(NO)during the estrous cycles,early pregnancy and abortion in goats[J].Anin Reprod Sci,2016,174:73-79.
[16]周素娴,夏宁,梁瑜桢,等.Graves眼病患者T细胞亚群的研究[J].中华内分泌代谢杂志,2006,22:126-127.
[17]Ategbo JM,Grissa O,Yessoufou A,et al.Modulation of adipokines and cytokines in gestational diabetes and macrosomia[J].J Clin Endocrinol Metab,2006,91:4137-4143.
[18]Sudharshana Murthy KA,Bhandiwada A,Chandan SL,et al.Evaluation of oxidative stress and proinflammatory cytokines in gestational diabetes mellitus and their correlation with pregnancy outcome[J].Indian J Endocrinol Metab,2018,22:79-84.
[19]Mrizak I,Grissa O,Henault B,et al.Placental infiltration of inflammatory markers in gestational diabetic women[J].Gen Physiol Biophys,2014,33:169-176.
[20]Viardot A,Grwey ST,Mackay E,et al.Potential antiinflammatory role of insulin via the preferential polarization of effector T cells toward a T helper2 phenotype[J].Endocrinology,2007,148:346-353.
[21]King MR,Ismail AS,Davis LS,et al.Oxidative stress promotes polarization of human T cell differentiation toward a T helper 2phenotype[J].J Immunol,2006,176:2765-2772.