蒙药蓝盆花中化学成分的体外抗癌活性研究
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摘要
采用MTT法评价蒙药蓝盆花中十五种化合物的体外抗癌活性。结果显示,大多数化合物对MDCK、YAC-1、SMMC-7721具有不同程度的抑制作用,且呈剂量依赖性,高剂量50μg·mL-1时,对MDCK、YAC-1的抑制率高于50%的只有化合物11和16,而对SMMC-7721的抑制率高于50%的则有1、2、3、4、6、7、8、9、12、15和16;多数化合物对K562的抑制作用在中等剂量25μg·mL-1时最强,9、11、15和16的抑制率大于50%;化合物12对MDCK、K562、SMMC-7221的抑制作用无选择性,均居所有化合物之最;化合物6对MDCK、SMMC-7221的抑制率分别为41.18%、99.42%,表现出高选择性。结果表明,化合物1、2、3、4、6、7、8、9、12、15、16为蒙药蓝盆花的体外抗肝癌活性成分群;化合物12、16、6在药材质量控制中应予以关注。
The MTT assay was used to evaluate anti-tumor activity of fifteen compounds from the flowers of Scabiosa tschilliensis Grunning in vitro.Most compounds exhibited the different inhibitory effects with dose-dependent manner on MDCK,YAC-1 and SMMC-7721.At the high dose of 50μg·mL-1,only Comp. 11 and 16 showed the inhibition rate over 50% on MDCK and YAC-1,while Comp. 1、2、3、4、6、7、8、9、12、15 and 16 with the inhibition rate higher than 50% against SMMC-7721.The inhibitory effect of most compounds on K562 at the middle dose of 25μg·mL-1 was higher than that at high or low dose,Comp. 9,11,15 and 16 with the inhibition rates more than 50%. The inhibitory effect of comp. 12 on MDCK,K562 and SMMC-7221 was the highest among all compounds,so the compound didn't show selectivity. But Comp.6 exhibiting high selective inhibition against SMMC-7221,as its inhibitory rates on MDCK and SMMC-7221 were 41.18% and 99.42%,respectively. The results suggested that comp. 1、2、3、4、6、7、8、9、12、15 and 16 from the flowers of Scabiosa tschilliensis Grunning made up the components group with anti-hematoma effect in vitro;Comp. 12 and 16 should be paid attention to in the aspect of quality control.
The MTT assay was used to evaluate anti-tumor activity of fifteen compounds from the flowers of Scabiosa tschilliensis Grunning in vitro.Most compounds exhibited the different inhibitory effects with dose-dependent manner on MDCK,YAC-1 and SMMC-7721.At the high dose of 50μg·mL-1,only Comp. 11 and 16 showed the inhibition rate over 50% on MDCK and YAC-1,while Comp. 1、2、3、4、6、7、8、9、12、15 and 16 with the inhibition rate higher than 50% against SMMC-7721.The inhibitory effect of most compounds on K562 at the middle dose of 25μg·mL-1 was higher than that at high or low dose,Comp. 9,11,15 and 16 with the inhibition rates more than 50%. The inhibitory effect of comp. 12 on MDCK,K562 and SMMC-7221 was the highest among all compounds,so the compound didn't show selectivity. But Comp.6 exhibiting high selective inhibition against SMMC-7221,as its inhibitory rates on MDCK and SMMC-7221 were 41.18% and 99.42%,respectively. The results suggested that comp. 1、2、3、4、6、7、8、9、12、15 and 16 from the flowers of Scabiosa tschilliensis Grunning made up the components group with anti-hematoma effect in vitro;Comp. 12 and 16 should be paid attention to in the aspect of quality control.
引文
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[11]Ma JN,Bolraa S,Ji M,et al.Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S.tschilliensis.Natural Product Research,2016,30(5):590-594.
[12]Wang GY(王国英),Zhao ZL(赵子龙),Xue PF(薛培凤),et al.Chemical constituents from flowers of Scabiosa tschilliensis.Chin J Chin Mat Med(中国中药杂志),2015,40(5):807-813.
[13]Si TZHQ(司徒镇强),Wu ZHJ(吴军正).Cell culture.Xian:World Book Publishing House,2007.
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[15]Ou YH(欧阳辉),Li TE(黎田儿),He MZ(何明珍),et al.Identification of the Metabolites and Effective Components of Flos scabiosae Extract in Rats Using UHPLC-Q-Tof-MS/MS Method.Chin Pharm J(中国药学杂志),2016,51(14):1197-1203.
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[3]Zhonghuaencao Editing Committee of State TCM Administration.Chinese Material Medica.Mongolian Drugs(中华本草.蒙药卷).Shanghai:Shanghai Science and Technollog Press,2004:385.
[4]The Inner Mongolia Health Department.Inner Mongolia Mongolian medicine standard.Chifeng(内蒙古蒙成药标准),chifeng:Inner Mongolian Science and Technology Press,1984:285,287,299,307.
[5]Wang GY(王国英),Xue PF(薛培凤).The Research Progression of Traditional Mongolian Medicine of Lan Pen Hua and other Plants in Scabiosa.Acta Aca Med Neimongol(内蒙古医学院学报),2009,31(5):487-490.
[6]Zhu RG(珠日根),Li FQ(李福全).Advances on Chemical Constituents andPharmacological Effects of flowers from Scabiosa tschilliensis.Chin J Ethnomed Ethnoharm(中国民族民间医药),2012,21(2):4-5.
[7]Li TE(黎田儿),Ou YH(欧阳辉),Yang LJ(杨林军),et al.Research progress on chemical constituents of plants from Scabiosa L.and their pharmacological activities.Chin J Exp Tradit Med Form(中国实验方剂学杂志),2015,21(18):226-230.
[8]Yang HX(杨宏昕)Bai YF(白音夫),Mi GQ(米裕青),et al.Effect of Mongolia scabiosa acid on MMP-1,TIMP-1,TGF-β1and PDGF expression in model rats with experimental liver fibrosis.Northwest Pharm J(西北药学杂志),2017,32(4):477-480.
[9]Yang HX(杨宏昕),Dong MX(董珉翔),Mi GQ(米裕青),et al.Effect of Mongolia Scabious Acid on Serum MMP-1 and TIMP-1 Expression Levels in Experimental Liver Fibrosis Rats.Chin Pharm(中国药业),2014,23(19):37-38.
[10]Chang L(常亮),Yang HX(杨宏昕),Zhao XY(赵小原),et al.Study on the effect of Mongolia scabiosa acid on the prevention and treatment of liver injury in rats.J Med Pharm Chin Minorities(中国民族医药杂志),2012,18(2):44-45.
[11]Ma JN,Bolraa S,Ji M,et al.Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S.tschilliensis.Natural Product Research,2016,30(5):590-594.
[12]Wang GY(王国英),Zhao ZL(赵子龙),Xue PF(薛培凤),et al.Chemical constituents from flowers of Scabiosa tschilliensis.Chin J Chin Mat Med(中国中药杂志),2015,40(5):807-813.
[13]Si TZHQ(司徒镇强),Wu ZHJ(吴军正).Cell culture.Xian:World Book Publishing House,2007.
[14]Xu SHY(徐叔云),Bian R(卞如)Chen X(陈修).experimental methodology ofPharmacology(Second Edition).People’s Medical Publishing House,1991:1441.
[15]Ou YH(欧阳辉),Li TE(黎田儿),He MZ(何明珍),et al.Identification of the Metabolites and Effective Components of Flos scabiosae Extract in Rats Using UHPLC-Q-Tof-MS/MS Method.Chin Pharm J(中国药学杂志),2016,51(14):1197-1203.