氮离子束对黑稻光合性状的诱变效应
|
摘要
为了探索氮离子束对黑稻光合性状的诱变效应,以不同剂量梯度的氮离子束对信阳特种黑稻进行辐照,观察并分析其对黑稻的茎蘖动态和光合性状的影响。结果发现:离子束的注入抑制了茎蘖数的增加,降低了叶面积指数和产量,随着离子注入剂量的增加,表现出剂量效应;离子束辐照后,黑稻的株型指标发生了变化,叶绿素含量随着离子注入剂量的增加表现先上升后下降趋势,还出现了叶绿素突变体。研究证明:氮离子束对黑稻的光合性状具有显著的诱变效应,在黑稻诱变选育方面,氮离子束是一种有效的手段。
In order to explore the mutagenic effects of irradiation with nitrogen ion beam on photosynthetic characteristics of black rice,Xinyang special black rice was irradiated by different gradient doses of nitrogen ion beam,tiller dynamic changes were observed,and effects of photosynthetic traits of black rice were analyzed. This results showed that the injection of ion beam inhibited the increase of tillers and reduced the leaf area index. Further,as the ion implantation dose increased,those changes showed dosage effects. Some plant type indices of black rice were changed after ion beam irradiation. The chlorophyll content displayed the trend of first increased and then decreased as the ion implantation dose increased,and there were some chlorophyll mutant. This study proved that nitrogen ion beam had significant mutagenic effect on photosynthetic characteristics of black rice,which was an effective tool in the field of mutation breeding of black rice.
In order to explore the mutagenic effects of irradiation with nitrogen ion beam on photosynthetic characteristics of black rice,Xinyang special black rice was irradiated by different gradient doses of nitrogen ion beam,tiller dynamic changes were observed,and effects of photosynthetic traits of black rice were analyzed. This results showed that the injection of ion beam inhibited the increase of tillers and reduced the leaf area index. Further,as the ion implantation dose increased,those changes showed dosage effects. Some plant type indices of black rice were changed after ion beam irradiation. The chlorophyll content displayed the trend of first increased and then decreased as the ion implantation dose increased,and there were some chlorophyll mutant. This study proved that nitrogen ion beam had significant mutagenic effect on photosynthetic characteristics of black rice,which was an effective tool in the field of mutation breeding of black rice.
引文
[1]韩龙植,南钟浩,全东兴,等.特种稻种质创新与营养特性评价[J].植物遗传资源学报,2003,4(3):207-213.
[2]Noppawat Pengkumsri,Bhagavathi Sundaram Sivamaruthi,Sasithorn Sirilun,et al.Dietary supplementation of Thai black rice bran extract and yeast beta-glucan protects the dextran sodium sulphate mediated colitis induced rat[J].RSC Advances,2017,7(1):396-402.
[3]庞瑞华,黄雅琴,王景晨,等.特种稻的类别及信阳特种稻种质资源研究进展[J].湖北农业科学,2011,50(17):3461-3465.
[4]庞瑞华,王玲,李小宁,等.信阳地区水稻资源的遗传多样性分析[J].江苏农业科学,2016,44(7):113-116.
[5]张晓丽,王强,陈雷,等.东南亚特种水稻种质资源光合特性研究[J].植物遗传资源学报,2016,17(6):1008-1013.
[6]余增亮.离子束生物技术引论[M].合肥:安徽科学技术出版社,1998:195-222.
[7]余增亮.离子束与生命科学——一个新的研究领域[J].物理,1997,26(6):333-338.
[8]卫增泉,颉红梅,梁剑平,等.重离子束在诱变育种核分子改造中的应用[J].原子核物理评论,2003,20(1):38-41.
[9]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景[J].杂交水稻,2002,17(5):5-8.
[10]路淑霞,姬生栋,岳春辉,等.离子束介导的小麦变异株系染色体行为分析[J].河南师范大学学报,2011,39(1):165-168.
[11]程备久,田秋元,林毅,等.离子注入棉花诱变效应研究(II)离子注入对胚芽中同功酶和微核细胞率影响[J].安徽农学院学报,1991,18(2):148-152.
[12]黄群策,李新奇.离子束生物技术与稻属植物遗传改良研究[M].郑州:郑州大学出版社,2015.
[13]Benny Warman,Irfan Suliansyah,Etty Swasti,et al.Selection and Semi-Dwarf Allele Mutants Segregation Pattern as the Result of Gamma Ray Irradiation of West Sumatera Black Rice[J].International Journal on Advanced Science,Engineering and Information Technology,2015,5(5):362-365.
[14]冷语佳,钱前,曾大力.水稻理想株型的遗传基础研究[J].中国稻米,2014,20(2):1-6.
[15]吴世长,戴红燕,胡开伦,等.Co60-γ射线不同剂量辐射黑稻M1代性状研究[J].西昌学院学报:自然科学版,2007,21(1):13-17.
[16]夏英武,吴殿星.人工诱发的植物叶绿素突变体的遗传及应用[J].核农学通报,1996(1):42-44.
[2]Noppawat Pengkumsri,Bhagavathi Sundaram Sivamaruthi,Sasithorn Sirilun,et al.Dietary supplementation of Thai black rice bran extract and yeast beta-glucan protects the dextran sodium sulphate mediated colitis induced rat[J].RSC Advances,2017,7(1):396-402.
[3]庞瑞华,黄雅琴,王景晨,等.特种稻的类别及信阳特种稻种质资源研究进展[J].湖北农业科学,2011,50(17):3461-3465.
[4]庞瑞华,王玲,李小宁,等.信阳地区水稻资源的遗传多样性分析[J].江苏农业科学,2016,44(7):113-116.
[5]张晓丽,王强,陈雷,等.东南亚特种水稻种质资源光合特性研究[J].植物遗传资源学报,2016,17(6):1008-1013.
[6]余增亮.离子束生物技术引论[M].合肥:安徽科学技术出版社,1998:195-222.
[7]余增亮.离子束与生命科学——一个新的研究领域[J].物理,1997,26(6):333-338.
[8]卫增泉,颉红梅,梁剑平,等.重离子束在诱变育种核分子改造中的应用[J].原子核物理评论,2003,20(1):38-41.
[9]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景[J].杂交水稻,2002,17(5):5-8.
[10]路淑霞,姬生栋,岳春辉,等.离子束介导的小麦变异株系染色体行为分析[J].河南师范大学学报,2011,39(1):165-168.
[11]程备久,田秋元,林毅,等.离子注入棉花诱变效应研究(II)离子注入对胚芽中同功酶和微核细胞率影响[J].安徽农学院学报,1991,18(2):148-152.
[12]黄群策,李新奇.离子束生物技术与稻属植物遗传改良研究[M].郑州:郑州大学出版社,2015.
[13]Benny Warman,Irfan Suliansyah,Etty Swasti,et al.Selection and Semi-Dwarf Allele Mutants Segregation Pattern as the Result of Gamma Ray Irradiation of West Sumatera Black Rice[J].International Journal on Advanced Science,Engineering and Information Technology,2015,5(5):362-365.
[14]冷语佳,钱前,曾大力.水稻理想株型的遗传基础研究[J].中国稻米,2014,20(2):1-6.
[15]吴世长,戴红燕,胡开伦,等.Co60-γ射线不同剂量辐射黑稻M1代性状研究[J].西昌学院学报:自然科学版,2007,21(1):13-17.
[16]夏英武,吴殿星.人工诱发的植物叶绿素突变体的遗传及应用[J].核农学通报,1996(1):42-44.