叶用芥菜叶缘裂刻性状的遗传与相关基因表达分析
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摘要
以叶缘裂刻和叶缘锯齿的2种芥菜为试材,构建F_2和BC_1分离群体,对群体单株的裂刻性状进行调查,并对相关基因进行qRT-PCR分析,以期明确叶用芥菜叶缘裂刻性状的遗传规律,为芥菜育种提供参考依据。结果表明:芥菜叶缘裂刻性状由1对显性基因控制,将该基因命名为BjLB(Leaf-lobe in Brassica juncea);qRT-PCR分析结果表明,KNAT1基因在叶缘裂刻芥菜中的表达量高于叶缘锯齿芥菜的表达量,与预期结果一致,其它基因在2个亲本中表达差异不明显,推测KNAT1基因有可能是候选基因。
Two Brassica juncea mustard materials with leaf margin and leaf margin serration were used as materials,the F_2 and BC_1 segregating populations were constructed,the splicing traits of individual plants were investigated and qRT-PCR analysis of related genes was performed in order to clarify the inheritance of leaf margin traits of leaf mustard and provide theoretical basis for Brassica juncea breeding.The results showed that the leaf margin of the mustard leaf was controlled by a pair of dominant genes,and the gene was named BjLB(Leaf-lobe in Brassica juncea);qRT-PCR analysis showed that the expression level of KNAT1 gene in the leaf margin was higher than that of the leaf margin,and the other genes difference expressed in the two parents were not obvious,and it was speculated that the KNAT1 gene might be a candidate gene.
Two Brassica juncea mustard materials with leaf margin and leaf margin serration were used as materials,the F_2 and BC_1 segregating populations were constructed,the splicing traits of individual plants were investigated and qRT-PCR analysis of related genes was performed in order to clarify the inheritance of leaf margin traits of leaf mustard and provide theoretical basis for Brassica juncea breeding.The results showed that the leaf margin of the mustard leaf was controlled by a pair of dominant genes,and the gene was named BjLB(Leaf-lobe in Brassica juncea);qRT-PCR analysis showed that the expression level of KNAT1 gene in the leaf margin was higher than that of the leaf margin,and the other genes difference expressed in the two parents were not obvious,and it was speculated that the KNAT1 gene might be a candidate gene.
引文
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[2] SHYAM P.Brassica cytogenetics:A historical journey and my personal reminiscence[J].Chinese journal of oil crop sciences,2010,32(1):163-172.
[3] 涂金星,傅廷栋.甘蓝型油菜几个苗期形态标记的遗传[J].华中农业大学学报,2001(20):318-320.
[4] SHANI E,BEN-GERA H,SHLEIZER-BURKO S,et al.Cytokinin regulates compound leaf development in tomato[J].Plant Cell,2010(22):3206-3217.
[5] BILSBOROUGH G D,RUNIONS A,BARKOULAS M,et al.Model for the regulation of Arabidopsis thaliana leaf margin development[J].P Natl Acad SciUSA,2011,108:3424-3429.
[6] FLEISHON S,SHANI E,ORI N,et al.Negative reciprocal interactions between gibberellin and cytokinin in tomato[J].New Phytol,2011,190:609-617.
[7] YANAI O,SHALLI E,RUSS D,et al.Gibberellin partly mediates LANCEOLATE activity in tomato[J].Plant J,2011,68(4):571-82.
[8] 曾国平,曹寿椿.不结球白菜主要经济性状遗传规律的研究Ⅰ、11个质量性状的遗传分析[J].南京农业大学学报,1996,19(3):23-30.
[9] 刘静.萝卜败蕾的细胞形态学和小白菜裂叶性状分子标记研究[D].杨凌:西化农林科技大学,2008.
[10] 葛颂,洪德元.泡沙参复合体(桔梗科)的物种生物学研究III:性状的遗传变异及其分类价值[J].植物分类学报,1995,33(5):433-443.
[11] 王玉刚,李萌,吕晓雯,等.白菜叶裂数性状主基因+多基因遗传分析[J].西北植物学报,2012,32(2):252-256.
[12] 刘义华,冷蓉,张召荣,等.茎瘤芥叶片刺毛与缺刻的遗传分析[J].园艺学进展(第七辑),2006(1):666-669.
[13] LI F,KITASHIBA H,INABA K et al.A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits[J].DNA Research,2009(16):311-323.
[14] LOU P,ZHAO J,KIM J S,et al.Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa[J].Journal of Experimental Botany,2007,58:4005-4016.
[15] 邓杰,王辉,程锋,等.控制大白菜和白菜型油菜叶缘裂刻的 QTL 定位及分析[J].园艺学报,2012,39(4):661-668.
[16] ZHAO J J,PAULO M J,JAMAR D,et al.Association mapping of leaf traits flowering time and phytate content in Brassica rapa[J].Genome,2007,50:963-973.
[17] FENG L,KITASHIBA H,INABA K,et al.A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits[J].DNA Research,2009(16):311-323.
[18] 李婉莎,王春涛,胡向阳.拟南芥叶边缘锯齿状突变体的分离与鉴定[J].植物分类与资源学报,2012,34(1):28-32.
[19] BYRNE M,TIMMERMANS M,KIDNER C,et al.Development of leaf shape[J].Curr Opin Plant Biol,2001(4):38-43.
[20] GUO M,THOMAS J,COLLINS G,et al.Direct repression of KNOX loci by the asymmetric leaves complex of Arabidopsis[J].Plant Cell,2008(20):48-58.
[21] HAKE S,SMITH H M S,HOLTAN H,et al.The role of KNOX genes in plant development[J].Annu Rev Cell Dev Biol,2004(20):125-151.
[22] HAY A,BARKOULAS M,TSIANTIS M.Asymmetric leaves and auxin activities converge to repress brevipedicellus expression and promote leaf development in Arabidopsis[J].Develo Pment,2006,133:3955-3961.
[23] UCHIDA N,KIMURA S,KOENIG D,et al.Coordination of leaf development via regulation of KNOXI genes[J].J Plant Res,2010,123:7-14.
[24] VOLLBREEHT E,VEIT B,SINHA N,et al.The developmental gene knotted-1is a member of a maize homeobox gene family[J].Nature,1991,350:241-243.
[25] 余四斌.分子遗传图谱的构建[M].北京:科学出版社,2004:403-440.
[26] 霍雪萍,张琳萍,赵向绒,等.RNA 质量对内参基因选择和 qRT-PCR 结果评价的影响[J].生物技术通讯,2018,29(3):415-418.