应用正交设计优化冰菜扦插方法
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摘要
采用L_9(3~4)正交试验设计方法,探讨了插穗规格、生长调节剂种类和浓度3种因素对冰菜枝条扦插成活率的影响。结果表明:试验9个组合处理中,冰菜扦插成活率最高为80%;3种因素对冰菜枝条扦插成活率均有显著影响,插穗规格是影响扦插成活率的主要因子,其次是生长调节剂种类,生长调节剂浓度影响最小;随着冰菜枝条由二级侧枝(老枝条)向四级侧枝(嫩枝条)改变,扦插成活率呈逐步增加趋势;生长调节剂对试验指标的影响大小为GGR> IAA> NAA。冰菜扦插最佳方法是选用四级侧枝(粗度3～4 mm),50 mg·L~(-1)的GGR试剂浸泡处理1 h,扦插成活率达90.56%。
By using L_9(3~4) orthogonal experiment design method,this paper discussed the effects of 3 factors,namely cutting size,kinds of growth regulator and concentration,on the survival rate of ice vegetable branch cutting.The results showed that among the 9 combined treatments,the highest survival rate was 80%.All 3 factors had significant effects on the survival rate.The cutting size is the main factor affecting survival rate,followed by kinds of growth regulator.Regulator concentration had the least influence.As the ice plant shoots changed from the secondary lateral branch(old branch) to fourth lateral branch(tender branch),the survival rate showed the tendency of gradual increasing.The effect of growth regulator on the test indexes was GGR > IAA > NAA.The best cuttage method was to select the fourth lateral branch (3-4 mm) and soaked it in 50 mg·L~(-1) GGR for an hour.The survival rate could be up to 90.56%.
By using L_9(3~4) orthogonal experiment design method,this paper discussed the effects of 3 factors,namely cutting size,kinds of growth regulator and concentration,on the survival rate of ice vegetable branch cutting.The results showed that among the 9 combined treatments,the highest survival rate was 80%.All 3 factors had significant effects on the survival rate.The cutting size is the main factor affecting survival rate,followed by kinds of growth regulator.Regulator concentration had the least influence.As the ice plant shoots changed from the secondary lateral branch(old branch) to fourth lateral branch(tender branch),the survival rate showed the tendency of gradual increasing.The effect of growth regulator on the test indexes was GGR > IAA > NAA.The best cuttage method was to select the fourth lateral branch (3-4 mm) and soaked it in 50 mg·L~(-1) GGR for an hour.The survival rate could be up to 90.56%.
引文
曹艳云,潘月芳,黄志玲,彭玉华,郝海坤.2005.CPD生根粉在速生桉扦插育苗中的应用.湖南农业科学,19(1):54-55.
李培闪,刘小菊.2014.不同生根剂对玫瑰嫩枝扦插成活率的影响.现代园艺,(1):11-12.
廖恒登,陈明才,刘永贤,黄拔程.2008.不同植物生长调节剂处理对冬种免耕马铃薯生长特性与产量的影响.北京:中国作物学会马铃薯专业委员会2008年马铃薯大会.
刘宏涛.2005.园林花卉繁育技术.沈阳:辽宁科学技术出版社.
刘建辉,曾清如,杨德通.2013.双吉尔生根粉在金叶假连翘扦插中的应用研究.湖南农业科学,(8):30-31.
刘君,朱良其,张加正,朱长志,陈伟强,黑银秀.2016.浙江地区冰菜栽培技术.浙江农业科学,57(7):1089-1090.
申展,李铁华,文仕知,何功秀,黄守成,唐爱国.2013.不同因素对闽楠嫩枝扦插生根的影响.中南林业科技大学学报,33(1):63-67.
徐微风,覃和业,刘姣,王蕾,吕瑞,郭建春,符少萍,李瑞梅,胡新文,段瑞军.2017.冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化.江苏农业学报,33(4):775-781.
钟泰林,石柏林,钱奇霞,揭任娟.2003.几种草本花卉繁育试验.浙江林学院学报,20(1):108-110.
仲兆清,潘春香.2014.不同基质、外源激素与扦插时间对油茶扦插育苗的影响.南方农业学报,45(4):623-627.
朱永超,李彬,廖伟彪.2016.3种生长素对蓝叶忍冬枝条扦插生根的影响.草业科学,33(1):61-66.
Hailer M S,Barnes J D,Cushman J C,Borland A M.2012.ACAM-and starch-deficient mutant of the facultative CAM species Mesembryanthemum crystallinum reconciles sink demands by repartitioning carbon during acclimation to salinity.J Exp Bot,63(5):1985-1996.
Himabindu Y,Chakradhar T,Reddy M C,Kanygin A,Redding K E,Chandrasekhar T.2016.Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes.Environ Exp Bot,124:39-63.
李培闪,刘小菊.2014.不同生根剂对玫瑰嫩枝扦插成活率的影响.现代园艺,(1):11-12.
廖恒登,陈明才,刘永贤,黄拔程.2008.不同植物生长调节剂处理对冬种免耕马铃薯生长特性与产量的影响.北京:中国作物学会马铃薯专业委员会2008年马铃薯大会.
刘宏涛.2005.园林花卉繁育技术.沈阳:辽宁科学技术出版社.
刘建辉,曾清如,杨德通.2013.双吉尔生根粉在金叶假连翘扦插中的应用研究.湖南农业科学,(8):30-31.
刘君,朱良其,张加正,朱长志,陈伟强,黑银秀.2016.浙江地区冰菜栽培技术.浙江农业科学,57(7):1089-1090.
申展,李铁华,文仕知,何功秀,黄守成,唐爱国.2013.不同因素对闽楠嫩枝扦插生根的影响.中南林业科技大学学报,33(1):63-67.
徐微风,覃和业,刘姣,王蕾,吕瑞,郭建春,符少萍,李瑞梅,胡新文,段瑞军.2017.冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化.江苏农业学报,33(4):775-781.
钟泰林,石柏林,钱奇霞,揭任娟.2003.几种草本花卉繁育试验.浙江林学院学报,20(1):108-110.
仲兆清,潘春香.2014.不同基质、外源激素与扦插时间对油茶扦插育苗的影响.南方农业学报,45(4):623-627.
朱永超,李彬,廖伟彪.2016.3种生长素对蓝叶忍冬枝条扦插生根的影响.草业科学,33(1):61-66.
Hailer M S,Barnes J D,Cushman J C,Borland A M.2012.ACAM-and starch-deficient mutant of the facultative CAM species Mesembryanthemum crystallinum reconciles sink demands by repartitioning carbon during acclimation to salinity.J Exp Bot,63(5):1985-1996.
Himabindu Y,Chakradhar T,Reddy M C,Kanygin A,Redding K E,Chandrasekhar T.2016.Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes.Environ Exp Bot,124:39-63.