黑龙江省水稻纹枯病菌菌丝融合群判定及遗传多样性分析
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摘要
从黑龙江省27个县市采集分离到77个水稻纹枯病菌菌株,经显微镜观察全部为多核菌株。利用载玻片定位融合法,将分离纯化得到的77个菌株分别与AG1-1A、AG1-1C、AG3、AG5、AG-6和AG-8等6个标准菌株作对峙试验。结果表明,黑龙江省水稻纹枯病菌主要菌丝融合群为AG1-1A、AG1-1C和AG-5,出现频率分别是80.51%、12.99%、2.60%。营养亲合群判别结果表明,77个待测菌株分为23个营养亲和群,其中有11个亲和群由单个菌株独立组成,出现频率为47.83%。遗传变异分析中,当遗传距离为0.6667时,从不同菌丝融合群中选取的15个代表菌株可划分为5个类群,与菌丝融合群判定结果基本一致。
A total of 77 Rhizoctonia solani strains causing rice sheath blight were collected from27 counties in Heilongjiang Province, 77 strains by using microscope, and the results indicated that all the strains were multinuclear. The 77 isolates were further tested by using the method of slide positioning fusion with six standard strains, including AG1-1A, AG1-1C, AG3, AG5, AG-6, and AG-8.The results showed that the Rhizoctonia solani causing rice sheath blight in Heilongjiang Province were mainly in AG1-1A, AG1-1C and AG-5 fusion group, accounting for 80.51%, 12.99%, 2.60%, respectively.Vegetative compatibility results showed that 77 tested isolates were classified into the 23 vegetative compatibility groups,among which 11 groups were composed of a single strain, accounting for 47.83%.In the analysis of genetic variation, when the genetic distance was 0.6667, the 15 representative strains selected from different groups were divided into five groups, and the results were consistent with the results of the fusion group.
A total of 77 Rhizoctonia solani strains causing rice sheath blight were collected from27 counties in Heilongjiang Province, 77 strains by using microscope, and the results indicated that all the strains were multinuclear. The 77 isolates were further tested by using the method of slide positioning fusion with six standard strains, including AG1-1A, AG1-1C, AG3, AG5, AG-6, and AG-8.The results showed that the Rhizoctonia solani causing rice sheath blight in Heilongjiang Province were mainly in AG1-1A, AG1-1C and AG-5 fusion group, accounting for 80.51%, 12.99%, 2.60%, respectively.Vegetative compatibility results showed that 77 tested isolates were classified into the 23 vegetative compatibility groups,among which 11 groups were composed of a single strain, accounting for 47.83%.In the analysis of genetic variation, when the genetic distance was 0.6667, the 15 representative strains selected from different groups were divided into five groups, and the results were consistent with the results of the fusion group.
引文
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[8]刘霞,冯蕊,高达芳,等.云南省马铃薯黑痣病病原菌融合群鉴定及8种药剂对其的毒力[J].植物保护,2016,42(2):165-170.
[9]蒙姣荣,张超冲,李界秋,等.广西玉米纹枯病菌的菌丝融合群及其对杀菌剂的敏感性[J].中国农学通报,2006,22(7):452-454.
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[11]陈厚德,梁继农,朱华,等.江苏玉米纹枯病菌的菌丝融合群及其对药剂的敏感性[J].江苏农业学报,1997,13(2):94-98.
[12]唐朝荣,陈捷,纪明山,等.辽宁省玉米纹枯病病原学研究[J].植物病理学报,2000,30(4):319-326.
[13]蒙姣荣,张超冲,李界秋,等.广西水稻纹枯病菌菌丝融合群鉴定初报[J].中国农学通报,2006,22(6):327-329.
[14]冯典兴,郑爱萍,王世全,等.四川省不同寄主立枯丝核菌的遗传分化和致病力研究[J].植物病理学报,2005,35(6):520-525.
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[17]方正,陈怀谷,陈厚德,等.江苏省小麦纹枯病病原组成及其致病力研究[J].麦类作物学报,2006,26(1):117-120.
[18]王玲,黄雯雯,黄世文,等.浙皖鄂地区水稻纹枯病菌5个种群的遗传结构分析[J].生态学报,2010,30(20):5439-5447.
[19]叶兰钦,辛明明,杜金昆,等.SSR标记应用于烟草品种遗传多样性研究[J].中国农学通报,2009,25(1):56-62.
[20]王玲,左示敏,张亚芳,等.中国南方八省(自治区)水稻纹枯病菌群体遗传结构的SSR分析[J].中国农业科学,2015,48(13):2538-2548.
[21]刘翔,任佐华,陈娟芳,等.利用SSR分析湖南桃江病圃丽江新团黑谷上稻瘟病菌的遗传多样性[J].西南农业学报,2015,28(6):2496-2500.
[22]陈爱昌,魏周全,骆得功,等.甘肃省定西市马铃薯黑痣病菌菌丝融合群的鉴定及药剂筛选[J].植物保护,2016,42(1):197-202.
[23]陈延熙,张敦华,段霞渝,等.关于Rhizoctonia solani菌丝融合群分类和有性世代的研究[J].植物病理学报,1985(3):139-143.
[24]Seemal M,Sreenivas S S,Devaki N S.Identification of anastomosis group of Rhizoctonia solani associated with tobacco in Karnataka[J].Acta Biologica Indica,2014,3(2):654-658.
[25]田晓燕.马铃薯黑痣病菌菌丝融合群的鉴定[J].中国马铃薯,2011,25(5):298-301.
[26]郭强,崔一平,徐世强,等.不同地理来源的甘蔗梢腐病菌营养体亲和性研究[J].热带作物学报,2016,37(10):1949-1955.
[27]Kodama M,Qingyuan G,Inagaki K.Comparative distribution of vegetative compatibility groups of Rhizoctonia solani AG-1(1A)in neighboring paddy fields between 1992-1993 and 1998[J].Annals of the Phytopathological Society of Japan,2001,67(2):195.
[28]钟珊,张涛,杨俊,等.草莓丝核菌根腐病的病原菌鉴定[J].植物病理学报,2016,46(3):289-293.
[29]刘丽,张永军,许长征,等.一种改良的CTAB法提取产多糖真菌DNA[J].中国生物工程杂志,2014,34(5):75-79.
[30]温娜娜.基于SSR的玉米、水稻纹枯病菌(Rhizoctonia solani AG1-1A)群体遗传结构分析[D].泰安:山东农业大学,2012.
[31]杨静,刘海英,钱春荣,等.黑龙江省水稻品种SSR标记遗传多样性分析[J].东北农业大学学报,2008,39(6):1-10.
[32]邓先明.四川省西北稻区水稻纹枯病菌系研究[J].西南农业大学学报,1991,13(4):380-383.
[33]李祥,侯明生,胡汉桥,等.湖北省水稻纹枯病菌菌丝融合群鉴定[J].华中农业大学学报,1993,12(1):14-17.
[34]Liang J X,Qi J M,Fang P P,et al.Genetic diversity and genetic relatives analysis of tobacco germplasm based on inter-simple sequence repeat(ISSR)[J].Scientia Agricultura Sinica,2008,41(1):286-294.
[35]Lu G Y,Wu X M,Zhang D X,et al.SSR-based evaluation of distinctness and uniformity of rapeseed(Brassica napus L.)varieties under Chinese national official field tests[J].Scientia Agricultura Sinica,2008,41(1):32-42.
[36]Hua L,Yuan X P,Yu H Y,et al.A comparative study on SSRdiversity in Chinese major rice va-rieties planted in 1950s and during the most recent ten years[J].Chinese Journal of Rice Science,2007,21(2):150-154.
[37]Sun Y W,Li M S,Zhang D G,et al.Determine genetic diversity among 85 maize inbred lines using SSR markers[J].Journal of Maize Sciences,2007,15(6):19-26.
[38]Li Y H,Liiu Y,Guan R X,et al.Genetic structure and diversity of both enhanced germplasms developed during 10th Five-Year plan and modern cultivars released during 1963-1995 in China[J].Acta Agronomica Sinica,2007,33(10):1630-1636.
[2]李永刚,郝中娜,杨明秀,等.寒地水稻病害生防菌株L1的鉴定及抑菌机理研究[J].东北农业大学学报,2008,39(7):9-12.
[3]Kumar K V K,Yellareddygari S K,Reddy M S,et al.Efficacy of Bacillus subtilis MBI 600 against sheath blight caused by Rhizoctonia solani and on growth and yield of rice[J].Rice Science,2012,19(1):55-63.
[4]董金皋.农业植物病理学.第二版[M].北京:中国农业出版社,2010.
[5]Parmeter J R,Sher wood R T,Paltt W D.Anastomosis grouping among isolates of Thanatephorus cucumeris[J].Phytopathology,1969,59:1270-1278.
[6]Ogoshi A.Grouping of Rhizoctonia solani Kuhn and their perfect stages[J].Review of Plant Protection Research,1975(8):93-103.
[7]Li K M,Guo Q Y,Zhao L,et al.Study on the anastomosis groups of Rhizoctonia solani isolated from lucerne in Xinjiang and their pathogenicity[J].Pratacultural Science,2009,26(5):151-154.
[8]刘霞,冯蕊,高达芳,等.云南省马铃薯黑痣病病原菌融合群鉴定及8种药剂对其的毒力[J].植物保护,2016,42(2):165-170.
[9]蒙姣荣,张超冲,李界秋,等.广西玉米纹枯病菌的菌丝融合群及其对杀菌剂的敏感性[J].中国农学通报,2006,22(7):452-454.
[10]肖炎农,李建生,郑用链,等.湖北省玉米纹枯病病原丝核菌的种类和致病性[J].菌物系统,2002,21(3):419-424.
[11]陈厚德,梁继农,朱华,等.江苏玉米纹枯病菌的菌丝融合群及其对药剂的敏感性[J].江苏农业学报,1997,13(2):94-98.
[12]唐朝荣,陈捷,纪明山,等.辽宁省玉米纹枯病病原学研究[J].植物病理学报,2000,30(4):319-326.
[13]蒙姣荣,张超冲,李界秋,等.广西水稻纹枯病菌菌丝融合群鉴定初报[J].中国农学通报,2006,22(6):327-329.
[14]冯典兴,郑爱萍,王世全,等.四川省不同寄主立枯丝核菌的遗传分化和致病力研究[J].植物病理学报,2005,35(6):520-525.
[15]陈素清,吴斌,秦蓁,等.四川棉花立枯丝核菌(Rhizoctonia solani Klhn)菌丝融合群研究[J].西南农业学报,1998,12(1):56-60.
[16]喻大昭,杨小军,杨立军,等.湖北省小麦纹枯病病原菌菌丝融合群研究[J].湖北农业科学,2000(3):39-42.
[17]方正,陈怀谷,陈厚德,等.江苏省小麦纹枯病病原组成及其致病力研究[J].麦类作物学报,2006,26(1):117-120.
[18]王玲,黄雯雯,黄世文,等.浙皖鄂地区水稻纹枯病菌5个种群的遗传结构分析[J].生态学报,2010,30(20):5439-5447.
[19]叶兰钦,辛明明,杜金昆,等.SSR标记应用于烟草品种遗传多样性研究[J].中国农学通报,2009,25(1):56-62.
[20]王玲,左示敏,张亚芳,等.中国南方八省(自治区)水稻纹枯病菌群体遗传结构的SSR分析[J].中国农业科学,2015,48(13):2538-2548.
[21]刘翔,任佐华,陈娟芳,等.利用SSR分析湖南桃江病圃丽江新团黑谷上稻瘟病菌的遗传多样性[J].西南农业学报,2015,28(6):2496-2500.
[22]陈爱昌,魏周全,骆得功,等.甘肃省定西市马铃薯黑痣病菌菌丝融合群的鉴定及药剂筛选[J].植物保护,2016,42(1):197-202.
[23]陈延熙,张敦华,段霞渝,等.关于Rhizoctonia solani菌丝融合群分类和有性世代的研究[J].植物病理学报,1985(3):139-143.
[24]Seemal M,Sreenivas S S,Devaki N S.Identification of anastomosis group of Rhizoctonia solani associated with tobacco in Karnataka[J].Acta Biologica Indica,2014,3(2):654-658.
[25]田晓燕.马铃薯黑痣病菌菌丝融合群的鉴定[J].中国马铃薯,2011,25(5):298-301.
[26]郭强,崔一平,徐世强,等.不同地理来源的甘蔗梢腐病菌营养体亲和性研究[J].热带作物学报,2016,37(10):1949-1955.
[27]Kodama M,Qingyuan G,Inagaki K.Comparative distribution of vegetative compatibility groups of Rhizoctonia solani AG-1(1A)in neighboring paddy fields between 1992-1993 and 1998[J].Annals of the Phytopathological Society of Japan,2001,67(2):195.
[28]钟珊,张涛,杨俊,等.草莓丝核菌根腐病的病原菌鉴定[J].植物病理学报,2016,46(3):289-293.
[29]刘丽,张永军,许长征,等.一种改良的CTAB法提取产多糖真菌DNA[J].中国生物工程杂志,2014,34(5):75-79.
[30]温娜娜.基于SSR的玉米、水稻纹枯病菌(Rhizoctonia solani AG1-1A)群体遗传结构分析[D].泰安:山东农业大学,2012.
[31]杨静,刘海英,钱春荣,等.黑龙江省水稻品种SSR标记遗传多样性分析[J].东北农业大学学报,2008,39(6):1-10.
[32]邓先明.四川省西北稻区水稻纹枯病菌系研究[J].西南农业大学学报,1991,13(4):380-383.
[33]李祥,侯明生,胡汉桥,等.湖北省水稻纹枯病菌菌丝融合群鉴定[J].华中农业大学学报,1993,12(1):14-17.
[34]Liang J X,Qi J M,Fang P P,et al.Genetic diversity and genetic relatives analysis of tobacco germplasm based on inter-simple sequence repeat(ISSR)[J].Scientia Agricultura Sinica,2008,41(1):286-294.
[35]Lu G Y,Wu X M,Zhang D X,et al.SSR-based evaluation of distinctness and uniformity of rapeseed(Brassica napus L.)varieties under Chinese national official field tests[J].Scientia Agricultura Sinica,2008,41(1):32-42.
[36]Hua L,Yuan X P,Yu H Y,et al.A comparative study on SSRdiversity in Chinese major rice va-rieties planted in 1950s and during the most recent ten years[J].Chinese Journal of Rice Science,2007,21(2):150-154.
[37]Sun Y W,Li M S,Zhang D G,et al.Determine genetic diversity among 85 maize inbred lines using SSR markers[J].Journal of Maize Sciences,2007,15(6):19-26.
[38]Li Y H,Liiu Y,Guan R X,et al.Genetic structure and diversity of both enhanced germplasms developed during 10th Five-Year plan and modern cultivars released during 1963-1995 in China[J].Acta Agronomica Sinica,2007,33(10):1630-1636.