昭通小草坝天麻内生真菌的分离初探
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摘要
植物内生真菌能够参与植物活性成分的合成,是重要的活性次生代谢产物来源,本研究采用云南省昭通市彝良县小草坝天麻,利用组织分离法对天麻内生真菌进行分离培养,采用插片法对纯化后的菌丝进行显微观察,以期为天麻属内生真菌的分离提供一定的参考依据。
Endophytic fungi can participate in the synthesis of plant active components, which is an important source of active secondary metabolites. In this study, Xiaocaoba Gastrodia elata from Yiliang County, Zhaotong City, Yunnan Province, was used to isolate and culture endophytic fungi of Gastrodia elata, and the purified hyphal was observed by insert method in order to provide a certain reference for the isolation of endophytic fungi of Gastrodia.
Endophytic fungi can participate in the synthesis of plant active components, which is an important source of active secondary metabolites. In this study, Xiaocaoba Gastrodia elata from Yiliang County, Zhaotong City, Yunnan Province, was used to isolate and culture endophytic fungi of Gastrodia elata, and the purified hyphal was observed by insert method in order to provide a certain reference for the isolation of endophytic fungi of Gastrodia.
引文
[1]莫莉.天麻内生真菌的分离鉴定及药物化学的初步研究[D].贵阳:贵州大学,2009:1-74.
[2]唐科民.昭通天麻DNA条形码研究及药用成分分析[D].昆明:云南中医学院,2013.
[3]何晨,霍蕾,等.甘草的微生物转化[J].天然产物分离,2005,3(6):1-4.
[4]王兴红.鬼臼类植物内生真菌的分离及其抗癌活性研究[J].天然产物研究与开发,2006,18:723-727.
[5]胡凤,程玉鹏,王振月等.药用植物内生真菌研究现状及其应用前景[J].生物技术通讯,2008,19(5):781-783.
[6]孙桂丽,陈有为,夏国兴,等.云南重楼植物内生真菌的分离及抗菌活性筛选[J].微生物学杂志,2005,(6):59-62.
[7]Wei JC(魏景超).The Identification Handbook of Fungus(真菌鉴定手册).Shanghai:Shanghai Scientific and Technical Publishers,1979.
[8]Barnett HL,Hunter BB.Illustration of Deuteromycotina(半知菌属图解).Beijing:Scientific Press,1977.
[9]莫莉,康冀川,何劲等.天麻内生真菌菌群组成的初步研究[J].菌物研究,2008,6(4):211-215.
[10]毛益婷,代晓宇,等.不同生境下野生铁皮石斛内生真菌多样性的初步研究[J].新疆农业大学学报,2011,34(3):234~238.
[11]张博华,刘威,赵致,等.贵州仿野生栽培红天麻的生活史及物候期研究[J].中国中药杂志,2014,39(22):4311-4316.
[2]唐科民.昭通天麻DNA条形码研究及药用成分分析[D].昆明:云南中医学院,2013.
[3]何晨,霍蕾,等.甘草的微生物转化[J].天然产物分离,2005,3(6):1-4.
[4]王兴红.鬼臼类植物内生真菌的分离及其抗癌活性研究[J].天然产物研究与开发,2006,18:723-727.
[5]胡凤,程玉鹏,王振月等.药用植物内生真菌研究现状及其应用前景[J].生物技术通讯,2008,19(5):781-783.
[6]孙桂丽,陈有为,夏国兴,等.云南重楼植物内生真菌的分离及抗菌活性筛选[J].微生物学杂志,2005,(6):59-62.
[7]Wei JC(魏景超).The Identification Handbook of Fungus(真菌鉴定手册).Shanghai:Shanghai Scientific and Technical Publishers,1979.
[8]Barnett HL,Hunter BB.Illustration of Deuteromycotina(半知菌属图解).Beijing:Scientific Press,1977.
[9]莫莉,康冀川,何劲等.天麻内生真菌菌群组成的初步研究[J].菌物研究,2008,6(4):211-215.
[10]毛益婷,代晓宇,等.不同生境下野生铁皮石斛内生真菌多样性的初步研究[J].新疆农业大学学报,2011,34(3):234~238.
[11]张博华,刘威,赵致,等.贵州仿野生栽培红天麻的生活史及物候期研究[J].中国中药杂志,2014,39(22):4311-4316.