δ-catenin基因与非小细胞肺癌血管形成相关性
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摘要
目的探讨δ-catenin基因在非小细胞肺癌组织及癌旁组织中的表达及与血管形成的关系。方法选取89例非小细胞肺癌组织,应用免疫组织化学方法检测癌组织中δ-catenin、CD31、VEGF蛋白的表达,并分析δ-catenin与CD31、VEGF表达的相关性;建立转染si-δ-catenin的细胞系,采用三维培养细胞管状形成实验分析si-δ-catenin对肺癌A549细胞管状形成能力的影响。结果δ-catenin蛋白在78例非小细胞肺癌中高表达,并与CD31、VEGF的表达呈正相关。三维培养细胞管状形成实验的结果显示si-δ-catenin能够削弱A549细胞的管状形成能力。结论δ-catenin与非小细胞肺癌的血管形成相关。
Objective To explore the expression of δ-catenin and the relationship between the expression and the angiogenesis in non-small cell lung cancer and paracancerous tissue. Methods The expression of δ-catenin, CD31 and VEGF proteins in 89 cases of non-small cell lung cancer was detected by immunohistochemistry, and the correlation of δ-catenin expression to CD31 and VEGF was analyzed. The effect of si-δ-catenin on the tubule formation ability of lung cancer A549 cells(transfected with si-δ-catenin) was analyzed by three-dimensional culture. Results The overexpression of si-δ-catenin protein in non-small cell lung cancer was found, and positively correlated with the expression of CD31 and VEGF. The three dimensional tubular-forming experiments showed that si-δ-catenin weakened the tubular-forming ability of A549 cells. Conclusion δ-catenin is related to the angiogenesis of non-small cell lung cancer.
Objective To explore the expression of δ-catenin and the relationship between the expression and the angiogenesis in non-small cell lung cancer and paracancerous tissue. Methods The expression of δ-catenin, CD31 and VEGF proteins in 89 cases of non-small cell lung cancer was detected by immunohistochemistry, and the correlation of δ-catenin expression to CD31 and VEGF was analyzed. The effect of si-δ-catenin on the tubule formation ability of lung cancer A549 cells(transfected with si-δ-catenin) was analyzed by three-dimensional culture. Results The overexpression of si-δ-catenin protein in non-small cell lung cancer was found, and positively correlated with the expression of CD31 and VEGF. The three dimensional tubular-forming experiments showed that si-δ-catenin weakened the tubular-forming ability of A549 cells. Conclusion δ-catenin is related to the angiogenesis of non-small cell lung cancer.
引文
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[9]Zhang B,Niu W,Dong HY,et al.Hypoxia induces endothelial mesenchymal transition in pulmonary vascular remodeling.[J].Internat J Mol Med,2018,42(1):270-278.
[10]Itatani Y,Kawada K,Yamamoto T,et al.Resistance to antiangiogenic therapy in cancer-alterations to anti-VEGFpathway.[J].Internat J Mol Sci,2018,19(4):1232.
[11]Chen Y,Chen Y,Mathy NW,et al.The role of VEGF in the diagnosis and treatment of malignant pleural effusion in patients with non-small cell lung cancer[J].Mol Med Rep,2018,17(6):8019-8030.
[2]Ba?i?I,Karlo R,Zadro A?,et al.Tumor angiogenesis as an important prognostic factor in advanced non-small cell lung cancer(Stage IIIA)[J].Oncology Letters,2018,15(2):2335-2339.
[3]Chen Y,Chen Y,Mathy NW,et al.The role of VEGF in the diagnosis and treatment of malignant pleural effusion in patients with non-small cell lung cancer[J].Mol Med Rep,2018,17(6):8019-8030.
[4]Lu Q,Aguilar BJ,Li M,et al.Genetic alterations ofδ-catenin/NPRAP/Neurojungin(CTNND2):functional implications in complex human diseases[J].Human Genetics,2016,135(10):1-10.
[5]He Y,Ryu T,Shrestha N,et al.Interaction of EGFR toδ-catenin leads toδ-catenin phosphorylation and enhances EGFRsignaling[J].Scientific Reports,2016,6:21207-21212.
[6]Kondratiuk I,??ski S,Urbańska M,et al.GSK-3βand MMP-9Cooperate in the Control of Dendritic Spine Morphology[J].Mol Neurobiol,2017,54(1):200-211.
[7]丁丝露,李光.Netrins与血管生成的研究进展[J].解剖科学进展,2018,24(2):197-200.
[8]刘文静,薛一雪,王萍,等.miR-107对人脑微血管内皮细胞增殖、迁移和血管形成能力的影响[J].解剖科学进展,2017,23(3):284-287.
[9]Zhang B,Niu W,Dong HY,et al.Hypoxia induces endothelial mesenchymal transition in pulmonary vascular remodeling.[J].Internat J Mol Med,2018,42(1):270-278.
[10]Itatani Y,Kawada K,Yamamoto T,et al.Resistance to antiangiogenic therapy in cancer-alterations to anti-VEGFpathway.[J].Internat J Mol Sci,2018,19(4):1232.
[11]Chen Y,Chen Y,Mathy NW,et al.The role of VEGF in the diagnosis and treatment of malignant pleural effusion in patients with non-small cell lung cancer[J].Mol Med Rep,2018,17(6):8019-8030.