稠油解烃菌与乳化剂产生菌复配降解降黏机理
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摘要
油杆菌W3分离自江苏油田韦5井,能够以稠油为唯一碳源生长,并降解稠油。54℃好氧振荡培养7 d之后,稠油能够完全乳化分散到培养基中,降黏率达到了76.88%,降解速率达到了99.7 mg/d,原油族组分分析发现,稠油中饱和烃,芳香烃和胶质的含量分别降低了4.14%,6.77%和4.33%。该菌与另一株筛选得到的、能够合成乳化剂的地芽孢杆菌W12复配(V_(W3)∶V_(W12)=1∶1)后作用稠油,既能够增强稠油降解效果(降解速率加快,原油族组分含量进一步降低),又能够改善稠油的乳化分散状态(乳化稳定性增强,平均乳化粒径减小56.7%),此时的稠油的乳化黏度为20.59 MPa·s,仅为初始黏度的3.9%。这两株菌的复配增强了稠油降解和乳化的双重降黏效果,大幅提高了稠油的流动性。
Petrobacter sp. W3 which could utilize heavy oil as the sole carbon source was isolated from Wei5 block,Jiangsu oilfield. The degradation ratio of heavy oil could reach 99. 7 mg/d after 7-day aerobic culture in54 ℃. Under the same conditions,W3 could perfectlyemulsify the same volume of heavy oil,the viscosity of heavy oil reduced by 76. 88%,the content of Saturated hydrocarbon,aromatic hydrocarbon and resins decreased by4. 14%,6. 77% and4. 33%. When this oil was degraded by the combination of W3 and Geobacillus sp. W12( V_(W3)∶ V_(W12)=1∶ 1),the two strains not only enhance heavy oil degradation( accelerate the degradation ratio,petroleum component continue to decrease),but also to improve the oil emulsion stability( the average partial size of oil reduced by56. 7%). The viscosity of heavy oil was 20. 59 MPa·s,only 3. 9% of the original. The experiments of the two strains achieved dual effects of viscosity break by means of heavy oil biodegradation and biological emulsion,which can significantly improve the mobilityof heavy oil.
Petrobacter sp. W3 which could utilize heavy oil as the sole carbon source was isolated from Wei5 block,Jiangsu oilfield. The degradation ratio of heavy oil could reach 99. 7 mg/d after 7-day aerobic culture in54 ℃. Under the same conditions,W3 could perfectlyemulsify the same volume of heavy oil,the viscosity of heavy oil reduced by 76. 88%,the content of Saturated hydrocarbon,aromatic hydrocarbon and resins decreased by4. 14%,6. 77% and4. 33%. When this oil was degraded by the combination of W3 and Geobacillus sp. W12( V_(W3)∶ V_(W12)=1∶ 1),the two strains not only enhance heavy oil degradation( accelerate the degradation ratio,petroleum component continue to decrease),but also to improve the oil emulsion stability( the average partial size of oil reduced by56. 7%). The viscosity of heavy oil was 20. 59 MPa·s,only 3. 9% of the original. The experiments of the two strains achieved dual effects of viscosity break by means of heavy oil biodegradation and biological emulsion,which can significantly improve the mobilityof heavy oil.
引文
1韩松,付步飞,李畅,等.1株稠油降黏菌BIT-BS002的性质研究与矿场应用.微生物学杂志,2012;32(4):24-29Han Song,Fu Bufei,Li Chang,et al.Characteristics and field application of bio-surfactant produced by Bacillus subtilis BIT-BS002.Journal of Microbiolog,2012;32(4):24-29
2王新伟,蔡婷,刘宇,等.稠油重质组分微生物降解作用研究进展.生态环境学报,2013;22(7):1255-1262Wang Xinwei,Cai Ting,Liu Yu,et al.Research advances in microbial degradation of heavy components in heavy oil.Ecology and Environmental Sciences,2013;22(7):1255-1262
3李彩风,徐闯,冯云,等.以稠油为唯一碳源的降解菌株的筛选与评价.河北大学学报(自然科学版),2014;34(4):405-409Li Caifeng,Xu Chuang,Feng Yun,et al.Screening and evaluation of degrading bacteria using heavy oil as sole carbon source.Journal o Heibei University(Natural Science Edition),2014;34(4):405-409
4 Fatma Z F,Sami M,Abdelmalek B,et al.Naphthalene and crude oil degradation by biosurfactant producing Streptomyces spp.isolated from Mitidja plain soil(North of Algeria).International Biodeterioration&Biodegradation,2014;86:300-308
5王大威,张健,齐义彬,等.稠油降解菌的筛选及其对胶质降解作用.微生物学报,2012;52(3):353-359Wang Dawei,Zhang Jian,Qi Yibin,et al.Isolation of viscous-oil degrading microorganism and biodegradation to resin.Acta Microbiologica Sinica,2012;52(3):353-359
6冯海柱,程武刚,陈刚,等.生物表面活性剂提高采收率技术室内研究.当代化工,2015;44(2):243-248Feng Haizhu,Cheng Wugang,Chen Gang,et al.Indoor study on EOR with biosurfactants.Contemporary Chemical Industry,2015;44(2):243-248
7齐义彬,李红,曹美娜,等.一株多环芳烃降解菌吉2及其降解能力.化工学报,2015;66(3):1072-1079Qi Yibin,Li Hong,Cao Meina,et al.A PAH-degrading strain JI 2and its biodegradation potential ability.CIESC Journal,2015;66(3):1072-1079
8赵玲侠,高配科,曹美娜,等.大庆聚驱后油藏内源微生物群落结构解析与分布特征研究.环境科学,2012;33(2):289-296Zhao Lingxia,Gao Peike,Cao Meina,et al.Research on population structure and distribution characteristic of indigenous microorganism in post-polymer-flooding oil reservoir.Environmental Science,2012;33(2):289-296
9齐义彬,曹美娜,黄立信,等.嗜热解烃菌的组合降黏降解机理.科学技术与工程,2014;14(24):18-22Qi Yibin,Cao Meina,Huang Lixin,et al.Mechanism of crude oil viscosity reduction and degradation by thermophilic hydrocarbon-degrading bacteria combination.Science Technology and Engineering,2014;14(24):18-22
10冯俊生,刘杨丹.石油降解菌的分离鉴定及降解能力初步研究.科学技术与工程,2013;13(9):2616-2618Feng Junsheng,Liu Yangdan.Isolation and identification of degrading bacteria and preliminary study on their degradation ability.Science Technology and Engineering,2013;13(9):2616-2618
11 Kikuchi T,Hasumi K.Enhancement of reciprocal activation of prourokinase and plasminogen by the bacterial lipoprtide surfactins and iturin Cs.The Journal of Antibiotics,2003;56(1):34-37
12黄红玺,张峰,许明标,等.油包水乳状液稳定性影响因素分析.断块油气田,2009;16(6):99-101Huang Hongxi,Zhang Feng,Xu Mingbiao,et al.Main influence factors of water-in-oil emulsion stability.Fault-block Oil&Gas Field,2009;16(6):99-101
13 Zheng C G,Yu L,Huang L X,et al.Investigation of a hydrocarbon-degrading strain,Rhodococcus ruber Z25,for the potential o microbial enhanced oil recovery.Journal of Petroleum Science and Engineering,2012;81:49-56
14国家发展和改革委员会.SY/T5119-2008岩石中可溶有机物及原油族组分分析.北京:石油工业出版社,2008The National Development and Reform Commission.SY/T5119-2008 The soluble organic matter and oil component analysis in the rock.Beijing:Petroleum Industry Press,2008
15张川,袁超鹏,王胜,等.两性离子聚合物钻井液低温流变特性研究.科学技术与工程,2015;15(31):30-35Zhang Chuan,Yuan Chaopeng,Wang Sheng,et al.Study on rheological properties of low temperature amphoteric polymer drilling fluid.Science Technology and Engineering,2015;15(31):30-35
16 Chen B L,Wang Y S,Hu D F.Biosorption and biodegradation o polycyclic aromatic hydrocarbons in aqueous solutions by a consortium of white-rot fungi.Journal of Hazardous Materials,2010;179:845-851
17 Jain P S,Bari S B,Surana S J.Isolation of stigmasterol andγ-sitosterol from petroleum ether extract of woody stem of abelmischus manihot.Asian Journal of Biological Sciences,2009:1767-1773
18徐爽,黄志勇,路福平,等.高温产生物乳化剂菌的筛选及其功能分析.科技导报,2011;29(15):52-57Xu Shuang,Huang Zhiyong,Lu Fuping,et al.Screening bioemulsifier-produced microorganisms and its function analysis.Science&Technology Review,2011;29(15):52-57
19杜国丰.一株稠油降解菌的筛选及其产物乳化性能研究.环境科学与管理,2016;41(7):55-59Du Guofeng.Study on screening a strain off degrading bacteria using think oil and emulsifving properties of its metabolites.Environmenta Science and Management,2016;41(7):55-59
2王新伟,蔡婷,刘宇,等.稠油重质组分微生物降解作用研究进展.生态环境学报,2013;22(7):1255-1262Wang Xinwei,Cai Ting,Liu Yu,et al.Research advances in microbial degradation of heavy components in heavy oil.Ecology and Environmental Sciences,2013;22(7):1255-1262
3李彩风,徐闯,冯云,等.以稠油为唯一碳源的降解菌株的筛选与评价.河北大学学报(自然科学版),2014;34(4):405-409Li Caifeng,Xu Chuang,Feng Yun,et al.Screening and evaluation of degrading bacteria using heavy oil as sole carbon source.Journal o Heibei University(Natural Science Edition),2014;34(4):405-409
4 Fatma Z F,Sami M,Abdelmalek B,et al.Naphthalene and crude oil degradation by biosurfactant producing Streptomyces spp.isolated from Mitidja plain soil(North of Algeria).International Biodeterioration&Biodegradation,2014;86:300-308
5王大威,张健,齐义彬,等.稠油降解菌的筛选及其对胶质降解作用.微生物学报,2012;52(3):353-359Wang Dawei,Zhang Jian,Qi Yibin,et al.Isolation of viscous-oil degrading microorganism and biodegradation to resin.Acta Microbiologica Sinica,2012;52(3):353-359
6冯海柱,程武刚,陈刚,等.生物表面活性剂提高采收率技术室内研究.当代化工,2015;44(2):243-248Feng Haizhu,Cheng Wugang,Chen Gang,et al.Indoor study on EOR with biosurfactants.Contemporary Chemical Industry,2015;44(2):243-248
7齐义彬,李红,曹美娜,等.一株多环芳烃降解菌吉2及其降解能力.化工学报,2015;66(3):1072-1079Qi Yibin,Li Hong,Cao Meina,et al.A PAH-degrading strain JI 2and its biodegradation potential ability.CIESC Journal,2015;66(3):1072-1079
8赵玲侠,高配科,曹美娜,等.大庆聚驱后油藏内源微生物群落结构解析与分布特征研究.环境科学,2012;33(2):289-296Zhao Lingxia,Gao Peike,Cao Meina,et al.Research on population structure and distribution characteristic of indigenous microorganism in post-polymer-flooding oil reservoir.Environmental Science,2012;33(2):289-296
9齐义彬,曹美娜,黄立信,等.嗜热解烃菌的组合降黏降解机理.科学技术与工程,2014;14(24):18-22Qi Yibin,Cao Meina,Huang Lixin,et al.Mechanism of crude oil viscosity reduction and degradation by thermophilic hydrocarbon-degrading bacteria combination.Science Technology and Engineering,2014;14(24):18-22
10冯俊生,刘杨丹.石油降解菌的分离鉴定及降解能力初步研究.科学技术与工程,2013;13(9):2616-2618Feng Junsheng,Liu Yangdan.Isolation and identification of degrading bacteria and preliminary study on their degradation ability.Science Technology and Engineering,2013;13(9):2616-2618
11 Kikuchi T,Hasumi K.Enhancement of reciprocal activation of prourokinase and plasminogen by the bacterial lipoprtide surfactins and iturin Cs.The Journal of Antibiotics,2003;56(1):34-37
12黄红玺,张峰,许明标,等.油包水乳状液稳定性影响因素分析.断块油气田,2009;16(6):99-101Huang Hongxi,Zhang Feng,Xu Mingbiao,et al.Main influence factors of water-in-oil emulsion stability.Fault-block Oil&Gas Field,2009;16(6):99-101
13 Zheng C G,Yu L,Huang L X,et al.Investigation of a hydrocarbon-degrading strain,Rhodococcus ruber Z25,for the potential o microbial enhanced oil recovery.Journal of Petroleum Science and Engineering,2012;81:49-56
14国家发展和改革委员会.SY/T5119-2008岩石中可溶有机物及原油族组分分析.北京:石油工业出版社,2008The National Development and Reform Commission.SY/T5119-2008 The soluble organic matter and oil component analysis in the rock.Beijing:Petroleum Industry Press,2008
15张川,袁超鹏,王胜,等.两性离子聚合物钻井液低温流变特性研究.科学技术与工程,2015;15(31):30-35Zhang Chuan,Yuan Chaopeng,Wang Sheng,et al.Study on rheological properties of low temperature amphoteric polymer drilling fluid.Science Technology and Engineering,2015;15(31):30-35
16 Chen B L,Wang Y S,Hu D F.Biosorption and biodegradation o polycyclic aromatic hydrocarbons in aqueous solutions by a consortium of white-rot fungi.Journal of Hazardous Materials,2010;179:845-851
17 Jain P S,Bari S B,Surana S J.Isolation of stigmasterol andγ-sitosterol from petroleum ether extract of woody stem of abelmischus manihot.Asian Journal of Biological Sciences,2009:1767-1773
18徐爽,黄志勇,路福平,等.高温产生物乳化剂菌的筛选及其功能分析.科技导报,2011;29(15):52-57Xu Shuang,Huang Zhiyong,Lu Fuping,et al.Screening bioemulsifier-produced microorganisms and its function analysis.Science&Technology Review,2011;29(15):52-57
19杜国丰.一株稠油降解菌的筛选及其产物乳化性能研究.环境科学与管理,2016;41(7):55-59Du Guofeng.Study on screening a strain off degrading bacteria using think oil and emulsifving properties of its metabolites.Environmenta Science and Management,2016;41(7):55-59