辣椒单倍体育种技术及其在育种中的应用
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摘要
利用单倍体育种技术进行种质资源创新,可明显缩短育种时间、丰富种质资源、提高育种效率。介绍了单倍体育种技术以及在辣椒育种中的应用,概述了影响辣椒单倍体植株诱导和分化的主要影响因素,旨为相关研究提供参考。
Using haploid breeding technology to innovate germplasm resources can significantly shorten breeding time, enrich germplasm resources, and improve breeding efficiency. Haploid breeding technology and its application in pepper breeding, as well as the main factors affecting the induction and differentiation of haploid plants in pepper were summarized in this paper, with the aim of providing references for related researches.
Using haploid breeding technology to innovate germplasm resources can significantly shorten breeding time, enrich germplasm resources, and improve breeding efficiency. Haploid breeding technology and its application in pepper breeding, as well as the main factors affecting the induction and differentiation of haploid plants in pepper were summarized in this paper, with the aim of providing references for related researches.
引文
[1]刘冰江,霍雨猛,杨妍妍,吴雄.蔬菜单倍体离体诱导及其在遗传育种中的应用[J].山东农业科学,2018,50(2):151-157
[2]Forster B P,Heberle-Bors E,Kasha K J,Touraev A.The resurgence of haploids in higher plants[J].Trends Plant Sci,2007,12(8):368-375
[3]Dunwell J M.Haploids in flowering plants:origins and exploitation[J].Plant Biotechnology Journal,2010,8(4):377-424
[4]Dwivedi S L,Britt A B,Tripathi L,Sharma S,Upadhyaya H D,Ortiz R.Haploids:Constraints and opportunities in plant breeding[J].Biotechnology Advances,2015,33(6):812-829
[5]Tsukazaki H,Kuginuki Y,Aida R,Suzuki T.Agrobacterium-mediated transformation of a doubled haploid line of cabbage[J].Plant Cell Reports,2002,21(3):257-262
[6]Cogan N,Harvey E,Robinson H,Lynn J,Pink D,Newbury H J,Puddephat I.The effect of anther culture and plant genetic background on Agrobacterium rhizogenesmediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea[J].Plant Cell Reports,2001,20(8):755-762
[7]殷丽琴,付绍红,杨进,李云,王继胜,王茂林.植物单倍体的产生、鉴定、形成机理及应用[J].遗传,2016,38(11):979-991
[8]Karimi-Ashtiyani R,Ishii T,Niessen M,Stein N,Heckmann S,Gurushidze M,Banaei-Moghaddam AM,Fuchs J,Schubert V,Koch K,Weiss O,Demidov D,Schmidt K,Kumlehn J,Houben,A.Point mutation impairs centromeric CENH3 loading and induces haploid plants[J].Proc Natl Acad Sci USA,2015,112(36):11211-11216
[9]Guha S,Maheshwari S C.In vitro Production of Embryos from Anthers of Datura[J].Nature,1964,204:497
[10]Seguisimarro J M.Doubled haploidy in model and recalcitrant species[J].Frontiers in Plant Science,2015,6:1175
[11]Maluszynski M,Kasha K J,Szarejko I.Published doubled haploid protocols in plant species[M].Springer Netherlands,2003
[12]Dunwell J M.Recent advances in the application of in vitro systems to plant improvement[J].Acta Horticulturae,2010,829:23-31
[13]Srivastava P,Chaturvedi R.In vitro androgenesis in tree species:An update and prospect for further research[J].Biotechnology Advances,2008,26(5):482-491
[14]Pickersgill B.Genetic resources and breeding of Capsicum spp.[J].Euphytica,1997,96(1):129-133
[15]王立浩,张正海,毛胜利,曹亚从,堵玫珍,张宝玺.甜椒抗番茄斑点萎蔫病毒的种质创新[J].中国蔬菜,2016,1(2):19-23
[16]李浚明,朱登云.植物组织培养教程[M].北京:中国农业大学出版社,2005
[17]Guha S,Maheshwari S C.Cell division and differentiation of embryos in the pollen grains of Datura in vitro[J].Nature,1966,212:97-98
[18]王玉英,孙敬三,王敬驹,钱南芬.小黑麦(Triticale)和辣椒(Capsicum annuum)花粉植株的诱导[J].中国科学,1973(1):104-107
[19]George L,Narayanaswamy S.Haploid Capsicum,through experimental androgenesis[J].Protoplasma,1973,78(4):467-470
[20]Shmykova N A,Pyshnaya O N,Shumilina D V,Dzhos E A.Morphological characteristics of doubled haploid plants of pepper produced using microspore/anther in vitro culture of the interspecies hybrids of Capsicum annum L.and C.chinense Jacq[J].Russian Agricultural Sciences,2014,40(6):417-421
[21]邢永萍,张树根,李春林,王振泉,张军民,蒋钟仁,李春玲.甜椒新品种海丰16号的选育[J].中国蔬菜,2014,1(6):49-51
[22]张树根,邢永萍,张军民,王振泉,邓晓梅,蒋钟仁,李春玲.辣椒新品种海丰1052[J].中国蔬菜,2015,1(5):63-65
[23]Chen J F,Cui L,Malik A A,Mbira K G.In vitro haploid and dihaploid production via unfertilized ovule culture[J].Plant Cell Tissue and Organ Culture,2011,104(3):311-319
[24]王仲慧,刘金兵,刁卫平,王述彬,潘宝贵,戈伟.辣椒花药培养胚状体发生途径影响因子研究[J].核农学报,2015,29(8):1471-1478
[25]Pawe?Nowaczyk,Anna Kisia?a.Effect of selected factors on the effectiveness of Capsicum annuum L.anther culture[J].Journal of Applied Genetics,2006,47(2):113-117
[26]Kristiansen K,Andersen S B.Effects of donor plant temperature,photoperiod,and age on anther culture response of Capsicum annuum L[J].Euphytica,1993,67(1-2):105-109
[27]张嘉园,辛鑫,刘亚忠,李秋孝,邢泽农.花药培养技术在辣椒育种中的应用[J].中国种业,2018(6):23-26
[28]张正海,毛胜利,王立浩,张宝玺.辣椒单倍体离体诱导及育种应用[J].园艺学报,2012,39(9):1715-1726
[29]胡梅,高杰,葛菊芬,许建,朱君芳,王娜.辣椒花蕾长度和单核靠边期花粉细胞比例间的关系[J].天津农业科学,2015,21(9):10-12
[30]Kim M,Jang I C,Kim J A,Park E J,Yoon M,Lee Y.Embryogenesis and plant regeneration of hot pepper(Capsicum annuum L.)through isolated microspore culture[J].Plant Cell Reports,2008,27(3):425-434
[31]张子君,徐矿红,田云,吴振红.辣椒花药培养诱导胚状体成苗[J].辽宁农业科学,2000(4):43-45
[32]陈晓,詹玉丝,徐小利,齐卫强,常高正,郅玉宝,易明林,吕淑玉.花药培养建立辣椒DH纯系的初步研究[J].河南农业科学,2003,32(9):52-55
[33]Parra-Vega V,Seguí-Simarro J M.Anther culture in pepper(Capsicum annuum L)[J].Methods in Molecular Biology,2016,1359(5):467-474
[34]NeftalíOchoa-Alejo.Anther culture of chili pepper(Capsicum spp.)[M]//Plant Cell Culture Protocols.Humana Press,2012
[35]王烨,张宝玺,连勇,王立浩.不同预处理对辣椒小孢子存活率的影响[J].中国蔬菜,2004,1(4):4-6
[36]Lantos C,AnikóGémes Juhász,Pál Vági,Róbert Mihály,Zoltán Kristóf,János Pauk.Androgenesis induction in microspore culture of sweet pepper(Capsicum annuum L.)[J].Plant Biotechnology Reports,2012,6(2):123-132
[37]孙少霞.辣椒(Capsicum annuum L.)花药、小孢子培养技术研究[D].南京:南京农业大学,2009
[38]王立浩,张宝玺,郭家珍,杨桂梅,堵玫珍.辣椒花药培养中若干影响因素的研究[J].园艺学报,2004,31(2):199-204
[39]Zhao J,Zou X X,Zhang Z Q,Yang B Z,Zhou S D.Influences of carbon sources and plant growth regulators on anther culture efficiency of pepper[J].Agricultural Science&Technology,2010,11(4):102-105
[40]Heidari A.Enhancement of microspore embryogenesis induction and plantlet regeneration of sweet pepper(Capsicum annuum L.)using putrescine and ascorbic acid[J].Proto plasma,2018(5)
[41]付文婷.辣椒单倍体培养主要影响因素研究进展[J].广东农业科学,2011,38(23):38-41
[42]李春玲,蒋仲仁.对甜椒花药培养中一些影响因素的研究[J].中国蔬菜,1983,1(2):35-37
[43]苏秀红.影响辣(甜)椒花药培养效率的因素研究[D].郑州:河南农业大学,2002
[44]李素文,黄亚杰,肖瑜,张斌.《辣椒花药离体培养》评述[J].中国蔬菜,2012,1(20):1-6
[45]陈斌,耿三省,张晓芬,刘凡.辣椒单倍体培养研究进展[J].长江蔬菜,2007(12):36-41
[46]赖黎丽,张嘉园,杨梅,宋文胜,朱华国.新疆加工型辣椒花药培养技术研究[J].北方园艺,2017(19):6-10
[47]陈薇,陈恩,庞基良.植物花粉培养研究进展[J].氨基酸和生物资源.2016,38(1):6-12
[2]Forster B P,Heberle-Bors E,Kasha K J,Touraev A.The resurgence of haploids in higher plants[J].Trends Plant Sci,2007,12(8):368-375
[3]Dunwell J M.Haploids in flowering plants:origins and exploitation[J].Plant Biotechnology Journal,2010,8(4):377-424
[4]Dwivedi S L,Britt A B,Tripathi L,Sharma S,Upadhyaya H D,Ortiz R.Haploids:Constraints and opportunities in plant breeding[J].Biotechnology Advances,2015,33(6):812-829
[5]Tsukazaki H,Kuginuki Y,Aida R,Suzuki T.Agrobacterium-mediated transformation of a doubled haploid line of cabbage[J].Plant Cell Reports,2002,21(3):257-262
[6]Cogan N,Harvey E,Robinson H,Lynn J,Pink D,Newbury H J,Puddephat I.The effect of anther culture and plant genetic background on Agrobacterium rhizogenesmediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea[J].Plant Cell Reports,2001,20(8):755-762
[7]殷丽琴,付绍红,杨进,李云,王继胜,王茂林.植物单倍体的产生、鉴定、形成机理及应用[J].遗传,2016,38(11):979-991
[8]Karimi-Ashtiyani R,Ishii T,Niessen M,Stein N,Heckmann S,Gurushidze M,Banaei-Moghaddam AM,Fuchs J,Schubert V,Koch K,Weiss O,Demidov D,Schmidt K,Kumlehn J,Houben,A.Point mutation impairs centromeric CENH3 loading and induces haploid plants[J].Proc Natl Acad Sci USA,2015,112(36):11211-11216
[9]Guha S,Maheshwari S C.In vitro Production of Embryos from Anthers of Datura[J].Nature,1964,204:497
[10]Seguisimarro J M.Doubled haploidy in model and recalcitrant species[J].Frontiers in Plant Science,2015,6:1175
[11]Maluszynski M,Kasha K J,Szarejko I.Published doubled haploid protocols in plant species[M].Springer Netherlands,2003
[12]Dunwell J M.Recent advances in the application of in vitro systems to plant improvement[J].Acta Horticulturae,2010,829:23-31
[13]Srivastava P,Chaturvedi R.In vitro androgenesis in tree species:An update and prospect for further research[J].Biotechnology Advances,2008,26(5):482-491
[14]Pickersgill B.Genetic resources and breeding of Capsicum spp.[J].Euphytica,1997,96(1):129-133
[15]王立浩,张正海,毛胜利,曹亚从,堵玫珍,张宝玺.甜椒抗番茄斑点萎蔫病毒的种质创新[J].中国蔬菜,2016,1(2):19-23
[16]李浚明,朱登云.植物组织培养教程[M].北京:中国农业大学出版社,2005
[17]Guha S,Maheshwari S C.Cell division and differentiation of embryos in the pollen grains of Datura in vitro[J].Nature,1966,212:97-98
[18]王玉英,孙敬三,王敬驹,钱南芬.小黑麦(Triticale)和辣椒(Capsicum annuum)花粉植株的诱导[J].中国科学,1973(1):104-107
[19]George L,Narayanaswamy S.Haploid Capsicum,through experimental androgenesis[J].Protoplasma,1973,78(4):467-470
[20]Shmykova N A,Pyshnaya O N,Shumilina D V,Dzhos E A.Morphological characteristics of doubled haploid plants of pepper produced using microspore/anther in vitro culture of the interspecies hybrids of Capsicum annum L.and C.chinense Jacq[J].Russian Agricultural Sciences,2014,40(6):417-421
[21]邢永萍,张树根,李春林,王振泉,张军民,蒋钟仁,李春玲.甜椒新品种海丰16号的选育[J].中国蔬菜,2014,1(6):49-51
[22]张树根,邢永萍,张军民,王振泉,邓晓梅,蒋钟仁,李春玲.辣椒新品种海丰1052[J].中国蔬菜,2015,1(5):63-65
[23]Chen J F,Cui L,Malik A A,Mbira K G.In vitro haploid and dihaploid production via unfertilized ovule culture[J].Plant Cell Tissue and Organ Culture,2011,104(3):311-319
[24]王仲慧,刘金兵,刁卫平,王述彬,潘宝贵,戈伟.辣椒花药培养胚状体发生途径影响因子研究[J].核农学报,2015,29(8):1471-1478
[25]Pawe?Nowaczyk,Anna Kisia?a.Effect of selected factors on the effectiveness of Capsicum annuum L.anther culture[J].Journal of Applied Genetics,2006,47(2):113-117
[26]Kristiansen K,Andersen S B.Effects of donor plant temperature,photoperiod,and age on anther culture response of Capsicum annuum L[J].Euphytica,1993,67(1-2):105-109
[27]张嘉园,辛鑫,刘亚忠,李秋孝,邢泽农.花药培养技术在辣椒育种中的应用[J].中国种业,2018(6):23-26
[28]张正海,毛胜利,王立浩,张宝玺.辣椒单倍体离体诱导及育种应用[J].园艺学报,2012,39(9):1715-1726
[29]胡梅,高杰,葛菊芬,许建,朱君芳,王娜.辣椒花蕾长度和单核靠边期花粉细胞比例间的关系[J].天津农业科学,2015,21(9):10-12
[30]Kim M,Jang I C,Kim J A,Park E J,Yoon M,Lee Y.Embryogenesis and plant regeneration of hot pepper(Capsicum annuum L.)through isolated microspore culture[J].Plant Cell Reports,2008,27(3):425-434
[31]张子君,徐矿红,田云,吴振红.辣椒花药培养诱导胚状体成苗[J].辽宁农业科学,2000(4):43-45
[32]陈晓,詹玉丝,徐小利,齐卫强,常高正,郅玉宝,易明林,吕淑玉.花药培养建立辣椒DH纯系的初步研究[J].河南农业科学,2003,32(9):52-55
[33]Parra-Vega V,Seguí-Simarro J M.Anther culture in pepper(Capsicum annuum L)[J].Methods in Molecular Biology,2016,1359(5):467-474
[34]NeftalíOchoa-Alejo.Anther culture of chili pepper(Capsicum spp.)[M]//Plant Cell Culture Protocols.Humana Press,2012
[35]王烨,张宝玺,连勇,王立浩.不同预处理对辣椒小孢子存活率的影响[J].中国蔬菜,2004,1(4):4-6
[36]Lantos C,AnikóGémes Juhász,Pál Vági,Róbert Mihály,Zoltán Kristóf,János Pauk.Androgenesis induction in microspore culture of sweet pepper(Capsicum annuum L.)[J].Plant Biotechnology Reports,2012,6(2):123-132
[37]孙少霞.辣椒(Capsicum annuum L.)花药、小孢子培养技术研究[D].南京:南京农业大学,2009
[38]王立浩,张宝玺,郭家珍,杨桂梅,堵玫珍.辣椒花药培养中若干影响因素的研究[J].园艺学报,2004,31(2):199-204
[39]Zhao J,Zou X X,Zhang Z Q,Yang B Z,Zhou S D.Influences of carbon sources and plant growth regulators on anther culture efficiency of pepper[J].Agricultural Science&Technology,2010,11(4):102-105
[40]Heidari A.Enhancement of microspore embryogenesis induction and plantlet regeneration of sweet pepper(Capsicum annuum L.)using putrescine and ascorbic acid[J].Proto plasma,2018(5)
[41]付文婷.辣椒单倍体培养主要影响因素研究进展[J].广东农业科学,2011,38(23):38-41
[42]李春玲,蒋仲仁.对甜椒花药培养中一些影响因素的研究[J].中国蔬菜,1983,1(2):35-37
[43]苏秀红.影响辣(甜)椒花药培养效率的因素研究[D].郑州:河南农业大学,2002
[44]李素文,黄亚杰,肖瑜,张斌.《辣椒花药离体培养》评述[J].中国蔬菜,2012,1(20):1-6
[45]陈斌,耿三省,张晓芬,刘凡.辣椒单倍体培养研究进展[J].长江蔬菜,2007(12):36-41
[46]赖黎丽,张嘉园,杨梅,宋文胜,朱华国.新疆加工型辣椒花药培养技术研究[J].北方园艺,2017(19):6-10
[47]陈薇,陈恩,庞基良.植物花粉培养研究进展[J].氨基酸和生物资源.2016,38(1):6-12