L-色氨酸分子印迹自组装膜的制备和应用研究
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摘要
以L-色氨酸为模板分子,十八烷基三氯硅烷(OTS)为功能单体,在构建L-色氨酸/OTS混合自组装膜和L-色氨酸分子印迹OTS自组装膜的基础上,完成了L-色氨酸印迹膜电化学传感器的制备与性能研究。结果表明L-色氨酸/OTS混合自组装膜形成后,覆盖在ITO玻璃表面,使其对电活性物质铁氰化钾的响应电流几乎为零,而空白ITO玻璃电极的响应电流很大,印迹膜修饰ITO玻璃电极的响应电流值介于前两者之间,说明印迹膜上有空穴存在,没有完全覆盖ITO玻璃表面;印迹自组装膜修饰电极对L-色氨酸分子的电流响应很大,而对与L-色氨酸分子结构相似的L-赖氨酸和L-亮氨酸的电流响应很小,且空白ITO玻璃电极对L-色氨酸分子的电流响应也很小,说明印迹膜对模板分子L-色氨酸分子具有专一识别性; L-色氨酸印迹膜传感器具有较低的检出限(1. 323μmol/L)和较高的灵敏度(3. 464μA/(μmol·L-1·cm2)),且传感器的重现性很好,储存时间较长。
The mixed L-tryptophan/octadecyltrichlorosilane( OTS) monolayer and L-tryptophanimprinted monolayer were prepared on ITO glass substrates using L-tryptophan as template molecule,octadecyltrichlorosilane as functional monomer by self-assembled monolayer( SAM) technique. The preparation and performance of the L-tryptophan-imprinted electrochemical sensor were also studied.The results indicate that the response current of the active substance ferricyanide is almost zero after the formation of L-tryptophan/OTS mixed self-assembled monolayer on the ITO glass surface. The bare ITO electrode has a high response current,and the response current value of the imprinted monolayer modified ITO glass electrode is medium,indicating the presence of the sites did not completely cover the surface of ITO glass. The imprinted monolayer electrode exhibits high current response to Ltryptophan molecules while L-lysine and L-leucine molecules that have similar structures with Ltryptophan molecules see a relative low current response. The current response of the bare ITO glass electrode to L-tryptophan molecules is also small,indicating that the molecularly imprinted monolayer has highly specific sensitive recognition of L-tryptophan. The L-tryptophan-imprinted sensor exhibits low detection limit of 1. 323 μmol/L and high sensitivity of 3. 464 μA/( μmol·L-1·cm2),and the sensor also shows a good reproducibility and long storage time.
The mixed L-tryptophan/octadecyltrichlorosilane( OTS) monolayer and L-tryptophanimprinted monolayer were prepared on ITO glass substrates using L-tryptophan as template molecule,octadecyltrichlorosilane as functional monomer by self-assembled monolayer( SAM) technique. The preparation and performance of the L-tryptophan-imprinted electrochemical sensor were also studied.The results indicate that the response current of the active substance ferricyanide is almost zero after the formation of L-tryptophan/OTS mixed self-assembled monolayer on the ITO glass surface. The bare ITO electrode has a high response current,and the response current value of the imprinted monolayer modified ITO glass electrode is medium,indicating the presence of the sites did not completely cover the surface of ITO glass. The imprinted monolayer electrode exhibits high current response to Ltryptophan molecules while L-lysine and L-leucine molecules that have similar structures with Ltryptophan molecules see a relative low current response. The current response of the bare ITO glass electrode to L-tryptophan molecules is also small,indicating that the molecularly imprinted monolayer has highly specific sensitive recognition of L-tryptophan. The L-tryptophan-imprinted sensor exhibits low detection limit of 1. 323 μmol/L and high sensitivity of 3. 464 μA/( μmol·L-1·cm2),and the sensor also shows a good reproducibility and long storage time.
引文
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[4] Yan M M,Zhao F N,She Y X,Hong S H,Zhang C,Cao X L,Wang M Q,Wang S S,Zheng L F,Jin M J,Shao H,Jin F,Liu H J,Wang J. Chin. J. Anal. Lab.,2018,37(5):607颜朦朦,赵风年,佘永新,洪思慧,张超,曹晓林,王猛强,王珊珊,郑鹭飞,金茂俊,邵华,金芬,刘海金,王静.分析试验室,2018,37(5):607
[5] Zhang Z J,Zhang X H,Liu B W,Liu J W. J. Am. Chem. Soc.,2017,139(15):5412
[6] Shen G J,Xiong D. J. Dalian Univ.,2014,35(6):53申贵隽,熊迪.大连大学学报,2014,35(6):53
[7] Wang M F,Lv Y Q,Liu X J,Qi W,Su R X,He Z M. ACS Appl. Mater. Interfaces,2016,8(22):14133
[8] Liu Y J. Preparation of two-dimensional SERS substrates with different configurations and detection study[D]. Ningbo University. 2017刘雅娇.不同构型二维SERS基底的制备及其检测研究[D].宁波大学. 2017年
[9] Suo A,Song L X,He J. J. Food Safe Qual Detec Technol,2014,5(11):3587索安,宋立新,何娟.食品安全质量检测学报,2014,5(11):3587
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[11] Teng Y,Su L Q,Wang Y Y,Han S. Chin. J. Anal. Lab.,2018,37(5):579滕跃,苏立强,王媛媛,韩爽.分析试验室,2018,37(5):579
[12] Wei X L,Qian Y X,Zhang Y F,Wang Z H. Chem Bull,2018,81(3):223魏小龙,钱亚眩,张亚芳,王志华.化学通报,2018,81(3):223
[13] Chen L,Li B. Food Chem.,2013,141:23
[14] Kitayama Y,Yoshikawa K,Takeuchi T. Macromolecules,2017,50(19):7526
[15] Zhang J,Cao D,Chen J M,Wang C Y. Materials Review,2013,27(10):55张进,曹丹,陈家美,王超英.材料导报,2013,27(10):55
[16] Li X,Zhang L M,Wu C R,Wei X P,Li J P. J. Instr. Anal. 2013,32(11):1344李雪,张连明,吴昌儒,魏小平,李建平.分析测试学报,2013,32(11):1344
[17] Amon A P,Lo D D,Montoro D T,Dewi R E,Longaker M T,Heilshorn S C. ACS Biomater. Sci. Eng.,2017,3(5):750
[18] Rajbhandary A,Raymond D M,Nilsson B L. Langmuir,2017,33(23):5803
[19] Huang W H,Tang H. Hot Working Technology,2017,46(16):158黄文恒,唐浩.热加工工艺,2017,46(16):158
[20] Qi W J,Zhang Y G,Liu Y,Song Q J. J. Anal. Sci. 2011,27(3):331齐文静,张义根,刘瑛,宋启军.分析科学学报,2011,27(3):331
[21] Prasad B B,Madhuri R,Tiwari M T,Sharma P S. Talanta,2010,81(1-2):187
[22] Wang C L,Hu X L,Guan P,Pu D. Membra. Sci. Technol. 2012,32(2):70王超丽,胡小玲,管萍,蒲东.膜科学与技术,2012,32(2):70
[23] Chen C,Yao C L,Yuan X S,Zhu J M. J. Instr. Anal. 2013,32(11):1333陈琛,姚成立,袁新松,朱金苗.分析测试学报,2013,32(11):1333