紫色叶用莴苣中花青素含量与色差指标的相关性
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摘要
【目的】建立高效快速的紫色叶用莴苣花青素含量检测方法。【方法】以4个紫色叶用莴苣品种为试材,利用分光光度计和色差仪分别测定其花青素含量和色差指标,并对6个色差指标与花青素含量进行相关与回归分析。【结果】结果表明:品种Z14中花青素含量最高,Z38最低;叶片色差指标中除h以外,L、a、b、C、H均能反映花青素含量变化;以5个色差指标值分别与花青素含量建立回归方程,其中H与花青素含量之间的回归方程相关系数最大,为0.806。【结论】成功建立依赖色差仪测定色差指标快速测定紫色叶用莴苣中花青素含量的体系。
【Objective】To establish a fast method for determining anthocyanin content in purple lettuce.【Methods】Four purple lettuce cultivars were selected as material,and the anthocyanin contentand color difference were analyzed using spectrophotometer and colorimeter.Correlation and regression analysis between the color difference parameters and content of anthocyanin was conducted.【Result】The results showed that highest content of anthocyanin was detected in Z14,while the lowest in Z38.Significant correlations were occurred between all the color difference parameters and content of anthocyanin except for h.Regression equation were established between anthocyanin content and five color difference parameters respectively.The correlation coefficient of regression equation between H and anthocyanin content was the highest(0.806).【Conclusion】Anthocyanin concentration in purple lettuce can be quickly determined by measuring color difference parameters using a colorimeter.
【Objective】To establish a fast method for determining anthocyanin content in purple lettuce.【Methods】Four purple lettuce cultivars were selected as material,and the anthocyanin contentand color difference were analyzed using spectrophotometer and colorimeter.Correlation and regression analysis between the color difference parameters and content of anthocyanin was conducted.【Result】The results showed that highest content of anthocyanin was detected in Z14,while the lowest in Z38.Significant correlations were occurred between all the color difference parameters and content of anthocyanin except for h.Regression equation were established between anthocyanin content and five color difference parameters respectively.The correlation coefficient of regression equation between H and anthocyanin content was the highest(0.806).【Conclusion】Anthocyanin concentration in purple lettuce can be quickly determined by measuring color difference parameters using a colorimeter.
引文
[1]VanisreeMulabagal,Mathieu Ngouajio,Ajay Nair,et al.NairaIn vitro evaluation of red and green lettuce(Lactuca sativa)for functional food properties[J].Food Chemistry,2010,118:300-306
[2]AparnaGazula.Anthocyanin Levels in Nine Lettuce(Lactuca sativa)Cultivars:Influence of Planting Date and Relations among Analytic,Instrumented,and Visual Assessments of Color[J].HORTSCIENCE,2007,42:232-238
[2]孙日飞,张淑江,章时蕃,等.紫红色大白菜种质的创新研究[J].园艺学报,2006,33:1032
[4]Zhang W,Seki M,Furusaki S.Effect of temperature and its shift on growth and anthocyanin production in suspension cultures of strawberry cells[J].Plant Science,1997,127:207-214
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[6]Kobayashi Y,Akita M,Sakamoto K,et al.Large-scale production of anthocyanin by Aralia cordatacell suspension cultures[J].Applied Microbiology&Biotechnology,1993,40:215-218
[7]Mori T,Sakurai M.Production of anthocyanin from strawberry cell suspension cultures;effects of sugar and nitrogen[J].Journal of Food Science,1994,59:588-593
[8]Sang-UK CHON,Hee-OCK BOO,Buk-GU HEO,ShelaGorinstein.Anthocyanin content and the activities of polyphenol oxidase,peroxidase and phenylalanine ammonia-lyase in lettuce cultivars[J].International Journal ofFood Sciences and Nutrition,2012,63:45-48
[9]Harnly JM,Doherty R,Beecher GR,et al.Flavonoid content of U.S.fruits,vegetables,and nuts[J].Journal of Agricultural&Food Chemistry,2006,54:9966-9977
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[11]李建伟.pH示差法测定山楂中花青素的含量[J].长治医学院学报,2015(3):177-179
[12]殷丽琴,彭云强,钟成,等.高效液相色谱法测定8个彩色马铃薯品种中花青素种类和含量[J].食品科学,2015,36(18):143-147
[13]白新祥,胡可,戴思兰,等.不同花色菊花品种花色素成分的初步分析[J].北京林业大学学报,2006(2):84-89
[14]邝敏杰,齐敏玉,何静仁.紫菜薹花色苷组分鉴定及其稳定性和抗氧化性[J].中国农业科学,2014,47(20):4067-4077
[15]王利群,戴雄泽.色差计在辣椒果实色泽变化检测中的应用[J].辣椒杂志,2009(3):23-26
[16]张圆圆,齐冬梅,刘辉,等.观赏向日葵的花色多样性及其与花青苷的关系[J].园艺学报,2008,35(6):863-868
[17]刘维信,娄艳.白菜品种叶片色泽参数相关和聚类分析[J].中国蔬菜,2014(4):35-38
[18]王美玲,艾希珍,丁飞,等.紫甘蓝和普通甘蓝光合特性的比较[J].园艺学报,2008,35(4):547-552
[19]唐偲琦,李绍波,郎春辉,等.紫甘薯中花青素类色素提取和分光光度法测定[J].现代预防医学,2013,40(9):1721-1723
[20]林文超,王德森,王海龙,等.大白菜花青素含量及色差指标相关性研究[J].青岛农业大学学报:自然科学版,2011,28(3):201-204
[21]国艳梅,杜永臣,王孝宣,等.利用色差仪估测番茄果实番茄红素含量的研究[J].中国蔬菜,2008(11):10-14
[2]AparnaGazula.Anthocyanin Levels in Nine Lettuce(Lactuca sativa)Cultivars:Influence of Planting Date and Relations among Analytic,Instrumented,and Visual Assessments of Color[J].HORTSCIENCE,2007,42:232-238
[2]孙日飞,张淑江,章时蕃,等.紫红色大白菜种质的创新研究[J].园艺学报,2006,33:1032
[4]Zhang W,Seki M,Furusaki S.Effect of temperature and its shift on growth and anthocyanin production in suspension cultures of strawberry cells[J].Plant Science,1997,127:207-214
[5]Wellmann E,Harzdina G,Grisebach H.Introduction of anthocyanin formation and enzymes related to its biosynthesis by UV light in cell cultures of Haplopappusgracilis[J].Phytochemistry,1976,15:913-915
[6]Kobayashi Y,Akita M,Sakamoto K,et al.Large-scale production of anthocyanin by Aralia cordatacell suspension cultures[J].Applied Microbiology&Biotechnology,1993,40:215-218
[7]Mori T,Sakurai M.Production of anthocyanin from strawberry cell suspension cultures;effects of sugar and nitrogen[J].Journal of Food Science,1994,59:588-593
[8]Sang-UK CHON,Hee-OCK BOO,Buk-GU HEO,ShelaGorinstein.Anthocyanin content and the activities of polyphenol oxidase,peroxidase and phenylalanine ammonia-lyase in lettuce cultivars[J].International Journal ofFood Sciences and Nutrition,2012,63:45-48
[9]Harnly JM,Doherty R,Beecher GR,et al.Flavonoid content of U.S.fruits,vegetables,and nuts[J].Journal of Agricultural&Food Chemistry,2006,54:9966-9977
[10]毛建霏,付成平,郭灵安,等.可见分光光度法测定紫甘薯总花青素含量[J].食品与发酵科技,2010,46:201-204
[11]李建伟.pH示差法测定山楂中花青素的含量[J].长治医学院学报,2015(3):177-179
[12]殷丽琴,彭云强,钟成,等.高效液相色谱法测定8个彩色马铃薯品种中花青素种类和含量[J].食品科学,2015,36(18):143-147
[13]白新祥,胡可,戴思兰,等.不同花色菊花品种花色素成分的初步分析[J].北京林业大学学报,2006(2):84-89
[14]邝敏杰,齐敏玉,何静仁.紫菜薹花色苷组分鉴定及其稳定性和抗氧化性[J].中国农业科学,2014,47(20):4067-4077
[15]王利群,戴雄泽.色差计在辣椒果实色泽变化检测中的应用[J].辣椒杂志,2009(3):23-26
[16]张圆圆,齐冬梅,刘辉,等.观赏向日葵的花色多样性及其与花青苷的关系[J].园艺学报,2008,35(6):863-868
[17]刘维信,娄艳.白菜品种叶片色泽参数相关和聚类分析[J].中国蔬菜,2014(4):35-38
[18]王美玲,艾希珍,丁飞,等.紫甘蓝和普通甘蓝光合特性的比较[J].园艺学报,2008,35(4):547-552
[19]唐偲琦,李绍波,郎春辉,等.紫甘薯中花青素类色素提取和分光光度法测定[J].现代预防医学,2013,40(9):1721-1723
[20]林文超,王德森,王海龙,等.大白菜花青素含量及色差指标相关性研究[J].青岛农业大学学报:自然科学版,2011,28(3):201-204
[21]国艳梅,杜永臣,王孝宣,等.利用色差仪估测番茄果实番茄红素含量的研究[J].中国蔬菜,2008(11):10-14