逆境环境对不同品种优质鸡血清免疫指标的影响
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摘要
试验通过在冷热温度环境下分析地方优质鸡血清免疫指标以及相关酶活性变化情况,旨在为选育抗逆性较强的地方优质鸡提供理论依据。试验选用160日龄健康济宁百日鸡、琅琊鸡、沂蒙鸡各9只,依次置于-5℃、15℃、35℃三种温度(湿度55%)的环境控制舱中饲养48h,测定血清中免疫球蛋白(IgA、IgG、IgM)以及谷胱甘肽过氧化物酶、超氧化物歧化酶、碱性磷酸酶活性指标。结果显示:沂蒙鸡在-5℃和35℃温度环境下IgA水平显著高于济宁百日鸡(P<0.05),而IgM水平显著高于琅琊鸡(P<0.05),并且在15℃温度环境下IgA水平显著高于琅琊鸡(P<0.05),IgM水平显著高于济宁百日鸡(P<0.05);-5℃、35℃时沂蒙鸡超氧化物歧化酶水平显著高于琅琊鸡(P<0.05);-5℃环境下碱性磷酸酶水平与15℃比较略有下降,但差异不显著(P>0.05),与35℃比较显著下降(P<0.05),并且品种间差异不明显(P>0.05)。研究结果表明,沂蒙鸡在不同温度环境下IgA、IgG、IgM水平显著高于济宁百日鸡和琅琊鸡,而冷热应激环境下沂蒙鸡抗氧化能力显著高于琅琊鸡。
This study was conducted to analyze the serum immunity index and related enzyme activity of local quality chicken in the environment of cold and heat, which was designed to provide theoretical basis for selecting and breeding local high-quality chicken with strong resistance. A total of twenty seven 160-day-old Jining Hundred chicken,Langyachicken and Yimeng chicken each of nine were assigned to three environmental chambers with maintaining the relative humidity at 55%, which was kept at -5 ℃, 15 ℃, 35 ℃ for 48 hoursrespectively to determinethe serum immunoglob-ulin(IgA, IgG, IgM) and glutathione peroxidase, superoxide dismutase, alkaline phosphatase activity indicators.These survey results indicate that the level of Ig A in Yimeng chicken was significantly higher than that of Jining Bairi chicken(P<0.05), while the level of Ig M was significantly higher than that of Langya chicken(P<0.05) at the 15 ℃ and35 ℃. Meanwhile, the level of IgA was significantly higher than that of Langya chicken(P<0.05), and the level of IgM was significantly higher than that of Jining Bairi chicken(P<0.05). The level of superoxide dismutase(sod) in Yimeng chicken were significantly higher than that of langya chicken(P<0.05) at-5 ℃ and 35 ℃. Furthermore, the level of alkaline phosphatase at-5 ℃ slight declines compared with temperature of 15 ℃, but the difference was not significant(P>0.05), however, it was significantly decreased compared with temperature of 35 ℃(P<0.05), and no obvious difference between varieties(P>0.05). The results indicated that the levels of Ig A, Ig G and Ig M in Yimeng chicken were higher than those of Jiningbairi chicken and Langya chicken in different temperature environment, and the antioxidant ability of Yimeng chicken was higher than that of Langya chicken under cold and hot stress environment.
This study was conducted to analyze the serum immunity index and related enzyme activity of local quality chicken in the environment of cold and heat, which was designed to provide theoretical basis for selecting and breeding local high-quality chicken with strong resistance. A total of twenty seven 160-day-old Jining Hundred chicken,Langyachicken and Yimeng chicken each of nine were assigned to three environmental chambers with maintaining the relative humidity at 55%, which was kept at -5 ℃, 15 ℃, 35 ℃ for 48 hoursrespectively to determinethe serum immunoglob-ulin(IgA, IgG, IgM) and glutathione peroxidase, superoxide dismutase, alkaline phosphatase activity indicators.These survey results indicate that the level of Ig A in Yimeng chicken was significantly higher than that of Jining Bairi chicken(P<0.05), while the level of Ig M was significantly higher than that of Langya chicken(P<0.05) at the 15 ℃ and35 ℃. Meanwhile, the level of IgA was significantly higher than that of Langya chicken(P<0.05), and the level of IgM was significantly higher than that of Jining Bairi chicken(P<0.05). The level of superoxide dismutase(sod) in Yimeng chicken were significantly higher than that of langya chicken(P<0.05) at-5 ℃ and 35 ℃. Furthermore, the level of alkaline phosphatase at-5 ℃ slight declines compared with temperature of 15 ℃, but the difference was not significant(P>0.05), however, it was significantly decreased compared with temperature of 35 ℃(P<0.05), and no obvious difference between varieties(P>0.05). The results indicated that the levels of Ig A, Ig G and Ig M in Yimeng chicken were higher than those of Jiningbairi chicken and Langya chicken in different temperature environment, and the antioxidant ability of Yimeng chicken was higher than that of Langya chicken under cold and hot stress environment.
引文
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[2]庄桂玉,朱本银.琅琊鸡资源调查与保种开发[J].中国畜牧业,2014(4):54-56.
[3]王亚平,龙君江,邢文丽,等.鲁南地区不同鸡种体尺、屠宰及肉质性状的测定与分析[J].黑龙江畜牧兽医,2013,45(3):31-34.
[4]李佃场,徐振浩,周卫东,等.鸡的热应激及其防治措施[J].中国家禽,2005,27(10):45-47.
[5]黄立.产蛋鸡热应激的防制[J].中国家禽,2004,26(14):19-20.
[6]李香.偏热环境对肉鸡行为、生理生化指标及av UCP m RNA表达影响[D].邯郸:河北工程大学,2017.
[7]黄淑成.热应激条件下内毒素对肉鸡免疫功能影响的研究[D].郑州:河南农业大学,2015.
[8]王伟.亚麻木脂素对鹅生长性能及免疫功能的影响[D].哈尔滨:东北农业大学,2007.
[9]王国明.羊主要先天性免疫指标的检测与分析[D].青岛:山东农业大学,2012.
[10]王大力.猪干扰素-γ基因多态性及与生产性能、免疫指标关联性分析[D].大庆:黑龙江八一农垦大学,2009.
[11]李明皓.两种杂交鹅生产性能及血清生化指标的研究[D].大庆:黑龙江八一农垦大学,2008.
[12]李菊,张日俊.益生素对肉仔鸡生长性能、屠体性状及肉品质的影响[J].动物营养学报,2007,19(4):372-378.
[13]马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,19(10):53-56.