苏打碱胁迫对玉米自交系苗期生长及生理特性的影响
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摘要
为进一步分析实验室前期获得的耐盐玉米自交系种质,本实验通过形态观察和生理指标测定来进行评价。结果表明,苏打碱胁迫7 d后,4个自交系株高均有所降低,只有郑58降低显著;郑58和WD郑58叶绿素含量显著提高,美-2叶绿素含量提高但不显著,黑玉米叶绿素含量略有降低但不显著;4个自交系丙二醛(MDA)含量均有所提高但都不显著,脯氨酸、可溶性糖含量和POD活性并没有显著上升。分析以上结果可能是实验胁迫强度或者胁迫时间不够,对这4个自交系细胞膜结构没有产生严重破坏,细胞没有产生过多的渗透调节物质以及保护酶来抵抗逆境胁迫。该实验结论可为以后玉米自交系盐碱胁迫鉴定提供参考。
In order to further analyze the salt-tolerant maize inbred lines, morphological observation and physiological detection were used in this study. The results showed that the height of 4 inbred lines decreased after 7 days of soda alkali stress, only‘Zheng 58'expressed significantly. Moreover, the chlorophyll content of‘Zheng 58'and‘WD Zheng 58'increased significantly, the chlorophyll content of‘Mei-2'increased but not significantly. In addition, the chlorophyll content of black maize decreased slightly but not significantly. Ultimately, the MDA content of the four inbred lines increased but not significantly; the content of proline and soluble sugar, the activity of POD did not increase significantly. The results implied that the stress intensity or stress time may be not enough to cause serious damage to the cell membrane structure, and the cell did not produce excessive osmotic regulators and protective enzymes to resist stress. The results can provide reference for identification maize inbred lines under soda alkali stress in the future.
In order to further analyze the salt-tolerant maize inbred lines, morphological observation and physiological detection were used in this study. The results showed that the height of 4 inbred lines decreased after 7 days of soda alkali stress, only‘Zheng 58'expressed significantly. Moreover, the chlorophyll content of‘Zheng 58'and‘WD Zheng 58'increased significantly, the chlorophyll content of‘Mei-2'increased but not significantly. In addition, the chlorophyll content of black maize decreased slightly but not significantly. Ultimately, the MDA content of the four inbred lines increased but not significantly; the content of proline and soluble sugar, the activity of POD did not increase significantly. The results implied that the stress intensity or stress time may be not enough to cause serious damage to the cell membrane structure, and the cell did not produce excessive osmotic regulators and protective enzymes to resist stress. The results can provide reference for identification maize inbred lines under soda alkali stress in the future.
引文
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[3]张磊,候云鹏,王立春.盐碱胁迫对植物的影响及提高植物耐盐碱性的方法[J].东北农业科学,2018,43(4):11-16.
[4]郝凤,刘晓静,张晓磊,等.混合盐碱胁迫对紫花苜蓿苗期氮磷吸收及生理特性的影响[J].中国沙漠,2015,35(5):1268-1274.
[5]于爽,杨新宇,高剑,等.盐碱胁迫对龙葵幼苗生长及叶绿素含量的影响[J].贵州农业科学,2018,46(7):131-134.
[6]朱志明.不同玉米自交系对盐胁迫的响应及其耐盐性鉴定[D].银川:宁夏大学,2017.
[7]常国伟,王越,邓杰,等.萌芽期玉米自交系耐苏打碱的等级评价[J].种子,2018,37(2):78-82.
[8]张治安,陈展宇.植物生理学实验技术[M].长春:吉林大学出版社,2008:50-64.
[9]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2015:274-277.
[10]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009:48-50.
[11]张华新,刘正祥,刘秋芳.盐胁迫下树种幼苗生长及其耐盐性[J].生态学报,2009,29(5):2263-2271.
[12]王佺珍,刘倩,高娅妮,等.植物对盐碱胁迫的响应机制研究进展[J].生态学报,2017,37(16):1-13.
[13]刘霞.Na2CO3胁迫对蚕豆幼苗生长及光合特性的影响[D].济南:山东师范大学,2008.
[14]赵可夫.植物抗盐生理[M].北京:中国科学技术出版社,1993:9-10.
[15]成铁龙,李焕勇,武海雯,等.盐胁迫下4种耐盐植物渗透调节物质积累的比较[J].林业科学研究,2015,28(6):826-832.
[16]王明华.改良剂对苏打盐碱土及玉米生理特性的影响[D].哈尔滨:东北农业大学,2016.
[17]张春霞,边鸣镝,于慧,等.碳酸钠胁迫对甜高粱种子萌发和幼苗期生理特性的影响[J].吉林农业大学学报,2011,33(2):134-138,143.
[18]付艳,高树仁,杨克军,等.盐胁迫对玉米耐盐系与盐敏感系苗期几个生理生化指标的影响[J].植物生理学报,2011,47(5):459-462.