澳洲坚果不定芽诱导及生根培养研究
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摘要
以澳洲坚果品种‘H2’幼苗的幼嫩茎段为外植体材料,分别采用WPM、MS、1/2MS培养基,分析添加不同植物生长调节剂及其配比对腋芽启动、增殖及生根的影响。结果表明:适合茎段腋芽启动的培养基为WPM+6-BA 2.0 mg/L+IBA 1.0 mg/L+AC 200 mg/L,诱导系数为3.51,腋芽萌发后生长健壮;最佳增殖培养基为MS+6-BA 2.0 mg/L+KT 0.5 mg/L,增殖系数达3.49;组培苗诱导生根培养基为1/2MS+6-BA 0.2 mg/L+IBA 0.5 mg/L+ABA 0.5 mg/L+AC 200 mg/L,生根率为42.2%。
The shoot segments of macadamia nut cultivar 'H2' were used as explant materials, and WPM, MS and 1/2 MS were used as the basic medium to study the effects of different exogenous hormones and their ratios on axillary bud initiation, proliferation and rooting of tissue cultured seedlings. The results show that suitable medium for axillary bud initiation is WPM + 6-BA 2.0 mg/L + IBA 1.0 mg/L + AC 200 mg/L, and the induction coefficient is 3.51. Meanwhile, the sprouts grow robustly after axillary buds germination. The optimal proliferation medium for axillary buds is MS + 6-BA 2.0 mg/L + KT 0.5 mg/L, and the proliferation coefficient reaches 3.49.The optimum rooting formula selected in this study is 1/2 MS + 6-BA 0.2 mg/L + IBA 0.5 mg/L + ABA 0.5 mg/L +AC 200 mg/L, the rooting rate is 42.2%.
The shoot segments of macadamia nut cultivar 'H2' were used as explant materials, and WPM, MS and 1/2 MS were used as the basic medium to study the effects of different exogenous hormones and their ratios on axillary bud initiation, proliferation and rooting of tissue cultured seedlings. The results show that suitable medium for axillary bud initiation is WPM + 6-BA 2.0 mg/L + IBA 1.0 mg/L + AC 200 mg/L, and the induction coefficient is 3.51. Meanwhile, the sprouts grow robustly after axillary buds germination. The optimal proliferation medium for axillary buds is MS + 6-BA 2.0 mg/L + KT 0.5 mg/L, and the proliferation coefficient reaches 3.49.The optimum rooting formula selected in this study is 1/2 MS + 6-BA 0.2 mg/L + IBA 0.5 mg/L + ABA 0.5 mg/L +AC 200 mg/L, the rooting rate is 42.2%.
引文
[1]贺熙勇,陶亮,柳觐,等.我国澳洲坚果产业概况及发展趋势[J].热带农业科技,2015,38(3):12-16.
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[4]Gitonga L,Gichuki S,Ngamau K,et al.Effect of explant type,source and genotype on in vitro shoot regeneration in Macadamia(Macadamia spp.)[J].Agricultural Biotechnology and Sustainable Development,2010,2(7):129-135.
[5]Cha-um S,Chanseetis C,Chintakovid W,et al.Promoting root induction and growth of in vitro macadamia(Macadamia tetraphylla"Keaau")plantlets using CO2-enriched photoautotrophic conditions[J].Plant Cell,Tissue and Organ Culture,2011,106:435-444.
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[8]柳觐,孔广红,倪书邦,等.澳洲坚果多倍体的化学诱导及流式细胞术鉴定[J].热带作物学报,2013,34(11):2138-2144.
[9]王小箐,李玲.植物生长调节剂在植物组织培养中的应用[M].北京:化学工业出版社,2002:36.
[10]Rout G R,Sahoo S.In vitro selection and plant regeneration of copper-tolerant plants from leaf explants of Nicotiana tabacum L.cv.'Xanthi'[J].Plant Breeding,2007,126(4):403-409.
[11]Bhalla P L,Mulwa R M S.Tissue culture and macadamia propagation[J].Acta Horticulturae,2003,616:343-346.
[12]费学谦,阙国宁,周立红.ZT、ABA等内源物质与杉树龄及其组培复壮的关系[J].林业科学研究,1992,5(3):317-320.
[13]郭敏敏,王清连,胡根海.利用高效液相色谱法分离和测定棉花组织培养过程种4种内源激素[J].生物技术通讯,2009,20(2):213-216.
[14]郑均宝,刘玉军,裴保华,等.几种木本植物插穗生根与内源IAA,ABA的关系[J].植物生理学报,1991,17(3):313-316.
[15]孟庆伟,高辉远.植物生物学[M].2版.北京:中国农业出版社,2017:199-240.
[16]周燕,高述民,李凤兰.胡杨不定根原基发生的分生细胞结构特征及内源激素变化分析[J].西北植物学报,2009,29(7):1342-1350.
[2]罗培四,何新华,韦巧云,等.澳洲坚果繁育技术研究进展[J].中国南方果树,2017,46(3):179-183.
[3]Oliveira J P S,Hakimi O,Murgu M,et al.Tissue culture and metabolome investigation of a wild endangered medicinal plant using high definition mass spectrometry[J].Plant Cell,Tissue and Organ Culture,2018,134(1):153-167.
[4]Gitonga L,Gichuki S,Ngamau K,et al.Effect of explant type,source and genotype on in vitro shoot regeneration in Macadamia(Macadamia spp.)[J].Agricultural Biotechnology and Sustainable Development,2010,2(7):129-135.
[5]Cha-um S,Chanseetis C,Chintakovid W,et al.Promoting root induction and growth of in vitro macadamia(Macadamia tetraphylla"Keaau")plantlets using CO2-enriched photoautotrophic conditions[J].Plant Cell,Tissue and Organ Culture,2011,106:435-444.
[6]肖再云.澳洲坚果的组织培养研究[J].热带农业科学,1999(4):12-14.
[7]郭凌飞,彭靖茹,覃剑峰,等.澳洲坚果组织培养研究初报[J].中国农学通报,2010(26):385-386.
[8]柳觐,孔广红,倪书邦,等.澳洲坚果多倍体的化学诱导及流式细胞术鉴定[J].热带作物学报,2013,34(11):2138-2144.
[9]王小箐,李玲.植物生长调节剂在植物组织培养中的应用[M].北京:化学工业出版社,2002:36.
[10]Rout G R,Sahoo S.In vitro selection and plant regeneration of copper-tolerant plants from leaf explants of Nicotiana tabacum L.cv.'Xanthi'[J].Plant Breeding,2007,126(4):403-409.
[11]Bhalla P L,Mulwa R M S.Tissue culture and macadamia propagation[J].Acta Horticulturae,2003,616:343-346.
[12]费学谦,阙国宁,周立红.ZT、ABA等内源物质与杉树龄及其组培复壮的关系[J].林业科学研究,1992,5(3):317-320.
[13]郭敏敏,王清连,胡根海.利用高效液相色谱法分离和测定棉花组织培养过程种4种内源激素[J].生物技术通讯,2009,20(2):213-216.
[14]郑均宝,刘玉军,裴保华,等.几种木本植物插穗生根与内源IAA,ABA的关系[J].植物生理学报,1991,17(3):313-316.
[15]孟庆伟,高辉远.植物生物学[M].2版.北京:中国农业出版社,2017:199-240.
[16]周燕,高述民,李凤兰.胡杨不定根原基发生的分生细胞结构特征及内源激素变化分析[J].西北植物学报,2009,29(7):1342-1350.