肺腺癌中FURIN、CEACAM6和MTA2的表达及意义
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摘要
目的探讨肺腺癌中弗林蛋白酶(FURIN)、癌胚抗原相关细胞黏附分子6(CEACAM6)和转移相关基因2(MTA2)蛋白的表达特征及临床意义。方法收集96例肺腺癌术后组织蜡块及临床资料作为观察组,收集正常肺组织70例作为对照组,应用免疫组化方法检测两组中FURIN、CEACAM6和MTA2的表达。结果 FURIN、CEACAM6和MTA2在两组中表达的阳性率比较差异均有统计学意义(P<0.05)。观察组中FURIN、CEACAM6和MTA2的表达与肿瘤的最大径、脉管浸润、TNM分期和Ki67的表达密切相关。观察组中FURIN和CEACAM6、FURIN和MTA2的表达均呈正相关性。结论肺腺癌术后组织中FURIN、CEACAM6和MTA2高表达,三者对病变的进展有明显促进作用,FURIN对CEACAM6和MTA2可能有正向调节作用。
Objective To investigate the expressions of FURIN,CEACAM6 and MTA2 in lung adenocarcinoma,as well as their clinical significance.Methods The study included 96 cases of lung adenocarcinoma as observation group,and 70 cases of normal lung tissue as control group.The protein expressions of FURIN,CEACAM6 and MTA2 were detected by immunohistochemical technique.Results The expressions of FURIN,CEACAM6 and MTA2 were significantly different between the two groups(P < 0.05).Their expressions were correlated with diameter,invasion,TNM stage and Ki67 expression in the observation group.Positive correlations between FURIN and CEACAM6 and FURIN and MTA2 were found in the observation group.Conclusion There exist higher expressions of FURIN,CEACAM6 and MTA2 in lung adenocarcinoma.They may promote the development of the neoplasm.
Objective To investigate the expressions of FURIN,CEACAM6 and MTA2 in lung adenocarcinoma,as well as their clinical significance.Methods The study included 96 cases of lung adenocarcinoma as observation group,and 70 cases of normal lung tissue as control group.The protein expressions of FURIN,CEACAM6 and MTA2 were detected by immunohistochemical technique.Results The expressions of FURIN,CEACAM6 and MTA2 were significantly different between the two groups(P < 0.05).Their expressions were correlated with diameter,invasion,TNM stage and Ki67 expression in the observation group.Positive correlations between FURIN and CEACAM6 and FURIN and MTA2 were found in the observation group.Conclusion There exist higher expressions of FURIN,CEACAM6 and MTA2 in lung adenocarcinoma.They may promote the development of the neoplasm.
引文
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[14]霍海峰,范宗宪,牟嘉砾,等.MTA2蛋白在喉鳞癌中的表达及其临床意义[J].现代肿瘤医学,2012,20(9):1809-1812.
[15]摆斌.胃癌中MTA2、MMP-7和MMP-14的表达关系[J].中国老年学杂志,2013,33(13):3085-3087.
[16]Chen RN,Huang YH,Lin YC,et al.Thyroid hormone promotes cell invasion through activation of FURIN expression in human hepatoma cell lines[J].Endocrinology,2008,149(8):3817-3831.
[17]Anders L,Mertins P,Lammich S,et al.FURIN-,ADAM10-,and gamma-secretase-mediated cleavage of a receptor tyrosine phosphatase and regulation of beta-catenin's transcriptional activity[J].Mol CeU Biol,2006,26(10):3917-3934.
[18]Lopez de Cicco R,Bassi DE,Zucker S,et al.Human carcinoma cell growth and invasiveness is impaired by the propeptide of the ubiquitous proprotein convertase FURIN[J].Cancer Res,2005,65(10):4162-4171.
[19]霍海峰,范宗宪,牟嘉砾,等.MTA2mRNA中喉鳞癌及癌旁不同距离组织中的表达研究[J].黑龙江医药科学,2012,35(5):15-16.
[20]Lee H,Ryu SH,Hong SS,et al.Overexpression of metastasisassociated protein 2 is associated with hepatocellular carcinoma size and differentiation[J].J Gastroenterol Hepatol,2009,24(8):1445-1450.
[2]Huang YH,Lin KH,Liao CH,et al.FURIN overexpression suppresses tumor growth and predicts a better postoperative disease-free survival in hepatocellular carcinoma[J].PLoS One,2012,7(7):e40738.
[3]Bassi DE,Mahloogi H,Al-Saleem L,et al.Elevated FURIN expression in aggressive human head and neck tumors and tumor cell lines[J].Mol Carcinog,2001,31(4):224-232.
[4]Fu J,Bassi DE,Zhang J,et al.Transgenic overexpression of the proprotein convertase FURIN enhances skin tumor growth[J].Neoplasia,2012,14(4):271-282.
[5]蔡永杰,赵兴波,李明江,等.CEACAM6在卵巢癌中的表达及临床意义[J].中国医药导报,2009,6(9):25-26.
[6]彭杰,蒋重骅,龙泓羽,等.癌胚抗原相关细胞黏附分子6在胰腺癌组织中的表达及其临床意义[J].中国现代医学杂志,2013,23(2):57-60.
[7]Cui Y,Niu A,Pestell R,et al.Metastasis-associated protein 2is a repressor of estrogen receptor alpha whose overexpression leads to estrogen-independent growth of human breast cancer cells[J].Mol Endocrinol,2006,20(9):2020-2035.
[8]孟宇宏,张建中.肺、胸膜、胸腺及心脏肿瘤病理学和遗传学[M].北京:人民卫生出版社,2006:32-35.
[9]Gawlik K,Shiryaev SA,Zhu W,et al.Autocatalytic activation of the FURIN zymogen requires removal of the emerging enzyme's N-terminus from the active site[J].PLoS One,2009,4(4):e5031.
[10]Huynh TT,Chan KS,Piva TJ.Effect of ultraviolet radiation on the expression of pp38MAPK and FURIN in human keratinocyte-derived cell lines[J].Photodermatol Photoimmunol Photomed,2009,25(1):20-29.
[11]李科,刘明华.癌胚抗原相关黏附分子6蛋白在肝细胞癌组织中的表达及临床意义[J].第三军医大学学报,2011,33(17):1883-1884.
[12]李华茹.老年大肠腺癌患者术后组织中CEACAM6和MMP-2的表达及对预后的意义[J].中国老年学杂志,2013,33(3):683-684.
[13]Yu W,Chen L,Yang YQ,et al.Cytochrome P450ω-hydroxylase promotes angiogenesis and metastasis by upregulation of VEGF and CEACAM6 in non-small cell lung cancer[J].Cancer Chemother Pharmacol,2011,68(3):619-629.
[14]霍海峰,范宗宪,牟嘉砾,等.MTA2蛋白在喉鳞癌中的表达及其临床意义[J].现代肿瘤医学,2012,20(9):1809-1812.
[15]摆斌.胃癌中MTA2、MMP-7和MMP-14的表达关系[J].中国老年学杂志,2013,33(13):3085-3087.
[16]Chen RN,Huang YH,Lin YC,et al.Thyroid hormone promotes cell invasion through activation of FURIN expression in human hepatoma cell lines[J].Endocrinology,2008,149(8):3817-3831.
[17]Anders L,Mertins P,Lammich S,et al.FURIN-,ADAM10-,and gamma-secretase-mediated cleavage of a receptor tyrosine phosphatase and regulation of beta-catenin's transcriptional activity[J].Mol CeU Biol,2006,26(10):3917-3934.
[18]Lopez de Cicco R,Bassi DE,Zucker S,et al.Human carcinoma cell growth and invasiveness is impaired by the propeptide of the ubiquitous proprotein convertase FURIN[J].Cancer Res,2005,65(10):4162-4171.
[19]霍海峰,范宗宪,牟嘉砾,等.MTA2mRNA中喉鳞癌及癌旁不同距离组织中的表达研究[J].黑龙江医药科学,2012,35(5):15-16.
[20]Lee H,Ryu SH,Hong SS,et al.Overexpression of metastasisassociated protein 2 is associated with hepatocellular carcinoma size and differentiation[J].J Gastroenterol Hepatol,2009,24(8):1445-1450.