原花青素对玉米赤霉烯酮诱导小鼠睾丸TM4支持细胞凋亡的保护作用
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摘要
为了研究原花青素对玉米赤霉烯酮诱导的小鼠睾丸支持细胞TM4凋亡的保护作用,试验采用体外培养小鼠睾丸支持细胞TM4,用MTT法分别测定原花青素(PC)、玉米赤霉烯酮(ZEA)对TM4细胞生长的影响,流式细胞术检测PC对ZEA诱导的TM4细胞凋亡的保护作用,并检测PC、ZEA引起的总超氧化物歧化酶(T-SOD)的活性变化及丙二醛(MDA)的含量变化。结果表明:PC可显著增加TM4细胞的存活率,抑制TM4细胞的凋亡,提高T-SOD活性,降低MDA含量;有效抑制ZEA诱导的TM4细胞凋亡,主要是通过抗氧化作用对经ZEA染毒的TM4细胞起到保护作用。
The study was aimed to investigate the protective effect of procyanidine( PC) on apoptosis of TM4 cells induced by the zearalenone( ZEA). The effect of the growth of TM4 cells of the PC and ZEA was determined in vitro culture of TM4 by using MTT method. The protective effects of PC on induced apoptosis of TM4 cells induced by ZEA were detected by using flow cytometry. The activities of total superoxide dismutase( T-SOD) and the content of malondialdehyde( MDA) were detected and analyzed by kit. The results showed that PC can significantly increase the survival rates of TM4 cells,and exhibit inhibitory effect of ZEA on the apoptosis of TM4 cells; PC improves the T-SOD activity,and reduces the high content of MDA in cells induced by ZEA. These data demonstrate that PC can effectively inhibit the apoptosis of TM4 cells induced by ZEA through the antioxidant effect of ZEA exposed TM4 cells.
The study was aimed to investigate the protective effect of procyanidine( PC) on apoptosis of TM4 cells induced by the zearalenone( ZEA). The effect of the growth of TM4 cells of the PC and ZEA was determined in vitro culture of TM4 by using MTT method. The protective effects of PC on induced apoptosis of TM4 cells induced by ZEA were detected by using flow cytometry. The activities of total superoxide dismutase( T-SOD) and the content of malondialdehyde( MDA) were detected and analyzed by kit. The results showed that PC can significantly increase the survival rates of TM4 cells,and exhibit inhibitory effect of ZEA on the apoptosis of TM4 cells; PC improves the T-SOD activity,and reduces the high content of MDA in cells induced by ZEA. These data demonstrate that PC can effectively inhibit the apoptosis of TM4 cells induced by ZEA through the antioxidant effect of ZEA exposed TM4 cells.
引文
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[3]董双,何剑斌,张炎炎,等.玉米赤霉烯酮对雄性小鼠生殖系统的毒性作用[J].畜牧与兽医,2016,48(6):25-29.
[4]姜淑贞,杨维仁,杨在宾.玉米赤霉烯酮的污染和残留及其作用机制[J].中国饲料,2011(2):41-44.
[5]KANG C,LEE H,YOO Y S,et al.Evaluation of oxidative DNA damage using an alkaline single cell gel electrophoresis(SCGE)comet assay,and the protective effects of N-Acetylcysteine amide on zearalenone-induced cytotoxicity in Chang liver cells[J].Toxicol Res,2013,29(1):43-52.
[6]韩建鑫,何剑斌,高锋,等.原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用[J].中国畜牧兽医,2016,43(2):402-406.
[7]CAPANOGLU E,de VOS R C,HALL R D,et al.Changes in polyphenol content during production of grape juice concentrate[J].Food Chem,2013,139(1/2/3/4):521-526.
[8]FRACASSETTI D,COSTA C,MOULAY L,et al.Ellagic acid deriveaives,ellagitannins,proanthocyanidins and other phenolics,vitamin C and antioxidant capacity of two powder products from camu-camu fruit(Myrciaria dubia)[J].Food Chem,2013,139(1/2/3/4):578-588.
[9]陈欢,马磊,黄茹,等.原花青素在对乙酰氨基酚诱导的急性肝损伤中的保护作用及机制[J].重庆医科大学学报,2015,40(3):358-361.
[10]张慧文,张玉,马超美.原花青素的研究进展[J].食品科学,2015,36(5):296-304.
[11]王亚军,刘青,郑王龙等.玉米赤霉烯酮对Leydig细胞凋亡及caspase-9、caspase-3蛋白表达的影响[J].中国兽医学报,2014,34(1):131-135.
[12]王建花,张炎炎,龙淼.玉米赤霉烯酮所致氧化应激的研究进展[J].畜牧与饲料科学,2015(9):30-32.
[13]ABID-ESSEFI S,OUANES Z,HASSEN W,et al.Cytotoxicity,inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone[J].Toxicol In Vitro,2004,18(4):467-474.
[14]张娣.葡萄籽中原花青素抗氧化效果研究进展[J].黑龙江生态工程职业学院学报,2014,27(2):17-18.
[15]ZHANG HUIWEN,YANG YUMEI,MA CHAOMEI,et al.Structures and antioxidant and intestinal disaccharidase inhibitory activities of A-type proanthocyanidins from peanut skin[J].J Agric Food Chem,2013,61(37):8814-8820.
[16]SERRA A T,ROCHA J,SEPODES B,et al.Evaluation of cardiovascular protective effect of different apple varieties-correlation of response with composition[J].Food Chem,2012,135(4):2378-2386.
[17]SCHULZKE J D,GITTER A H,MANKERTZ J,et al.Epithelial transport and barrier function in occluding-deficient mice[J].Biochim Biophys Acta,2005,1669(1):34-42.
[18]MARIN D E,PISTOL G C,NEAGOE I V,et al.Effects of zearalenone on oxidative stress and inflammation in weanling piglets[J].Food Chem Toxicol,2013,58:408-415.
[19]王雪.莱菔硫烷对镉暴露小鼠睾丸支持细胞保护机制的研究[D].沈阳:沈阳农业大学,2016.
[20]王亚军.玉米赤霉烯酮诱导大鼠Leydig细胞凋亡及自噬保护作用[D].扬州:扬州大学,2014.