摘要
目的探讨核转录因子E2相关因子2(Nrf2)在非小细胞肺癌(NSCLC)中的表达及临床意义。方法回顾性选取2013年7月至2016年12月在辽宁省肿瘤医院治疗的NSCLC患者组织标本72例,同时选取癌旁组织(距癌组织> 5 cm)标本作为对照。采用免疫组化染色和逆转录-聚合酶链反应(RT-PCR)检测Nrf2蛋白表达和mRNA表达,观察其与临床病理特征及预后的关系。结果 NSCLC组织Nrf2阳性表达率和Nrf2 mRNA表达分别为62. 50%和(1. 392±0. 223),明显高于癌旁组织(P <0. 05);肿瘤直径> 5 cm、TNM分期为Ⅲ~Ⅳ期、有淋巴结转移患者Nrf2蛋白阳性表达率分别为87. 88%、86. 36%和76. 19%,明显高于肿瘤直径≤5 cm、Ⅰ~Ⅱ期、无淋巴结转移患者(P <0. 05); Nrf2mRNA表达与患者临床病理特征无关(P> 0. 05); Nrf2蛋白表达和Nrf2 mRNA表达呈正相关(r_s=0. 366,P <0. 05);Nrf2蛋白阳性表达患者中位无进展生存时间和总生存时间分别为12个月(95%CI:9. 92~14. 08)和21个月(95%CI:19. 76~22. 25),明显低于Nrf2蛋白阴性表达者(P <0. 05)。结论 Nrf2在NSCLC患者中的表达明显升高,其与临床病理特征及预后有一定关系,值得进一步研究。
Objective To investigate the expression and clinical significance of Nrf2 in non-small cell lung cancer. Methods 72 specimens of NSCLC patients who were treated in our hospital from July 2013 to December 2016 were selected and the para cancerous tissue( From the cancer > 5 cm) was selected as the control,the expression of Nrf2 protein and Mrna was detected by immunohistochemical staining and RT-PCR,and the relationship with the clinicopathological features and prognosis was compared. Results The positive expression rate of Nrf2 and mRNA in NSCLC tissue were 62. 50% and 1. 392 ± 0. 223,which were significantly higher than that of para cancerous tissue( P < 0. 05). The expression of Nrf2 protein in patients with tumor diameter > 5 cm,TNM stage III-IV stage,lymph node metastasis were 87. 88%,86. 36% and76. 19%,significantly higher than the tumor diameter ≤5 cm,I ~ II,without lymph node metastasis( P < 0. 05). The expression of Nrf2 mRNA was not related to the clinicopathological features of the patients( P > 0. 05). The expression of Nrf2 protein was positively correlated with the expression of Nrf2 mRNA( rs= 0. 366,P < 0. 05). The median progression free survival time and overall survival time in patients with positive expression of Nrf2 protein were 12 months( 95% CI: 9. 92 ~ 14. 08) and 21 months( 95% CI: 19. 76 ~ 22. 25),which were significantly lower than that of negative expression of Nrf2 protein( P < 0. 05). Conclusion The expression of Nrf2 in NSCLC is obviously elevated,which has a certain relationship with the clinicopathological features and prognosis of the patients,is worth further study.
引文
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