不同大麻品种种子萌发期耐重金属铜胁迫能力评价
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摘要
探讨不同铜浓度对9个大麻品种种子萌发期的影响,并筛选出耐铜型品种,为今后重金属污染的治理、相关工作深入展开提供理论参考。采用纸床培养法,以巴马火麻(BM)、云麻1号(Y1)、云麻6号(Y6)、晋麻1号(J1)、皖麻1号(W1)、云麻5号(Y5)、庆麻1号(Q1)、云麻7号(Y7)、云晚6号(YW6)9个大麻品种为研究对象,设置不同铜(Cu2+)浓度(0、50、100、150、300、600、900 mg/L)进行种子萌发期胁迫试验,以600 mg/L Cu2+为筛选浓度,采用隶属函数法对9个不同大麻品种进行耐铜性评价。结果表明,不同浓度的铜溶液对9个大麻的各项指标均有影响,且随着胁迫强度的增强,发芽势、发芽率、发芽指数、活力指数、根长、芽长、鲜重、侧根数量均有明显下降,与对照相比差异显著,高浓度重金属铜对大麻萌发有抑制作用。利用隶属函数将9个大麻品种分类,其结果为:Y1、Q1、BM为低耐铜型,J1、Y5、W1为中耐铜型,Y7、Y6、YW6为高耐铜型。
This study explored the effect of different Cu~(2+) concentration on seed germination and seedling growth and compared the capability of Cu-tolerance among nine hemp varieties,and it provided theory for the soil ecological restoration in heavy metal Cu~(2+)pollution area and provided evidence for the further research on the effects of heavy metal on plant.Bama,Yunma 1,Yunma 6,Jinma1,Wanma1,Yunma 5,Qingma1,Yunma 7,Yunwan6 were used as materials in this study,nine industrial hemp cultivars under different Cu~(2+) stress conditions(0、50、100、150、300、600、900 mg/L) were determined,which were assessed using subordinate function methods under the stress of 600 mg/L Cu~(2+).The results showed that the germination energy,germination rate,germination index,vigor index,root length,shoot length,fresh weight,number of lateral root were all decreased with the increasing of Cu~(2+) stress.According to average subordinate function,different hemp Cu-tolerance were classified:Y1、Q1、BM were low Cu-tolerance cultivars,J1、Y5、W1 were middle Cu-tolerance cultivars,Y6、YW6、Y7 were high Cu-tolerance cultivars.
This study explored the effect of different Cu~(2+) concentration on seed germination and seedling growth and compared the capability of Cu-tolerance among nine hemp varieties,and it provided theory for the soil ecological restoration in heavy metal Cu~(2+)pollution area and provided evidence for the further research on the effects of heavy metal on plant.Bama,Yunma 1,Yunma 6,Jinma1,Wanma1,Yunma 5,Qingma1,Yunma 7,Yunwan6 were used as materials in this study,nine industrial hemp cultivars under different Cu~(2+) stress conditions(0、50、100、150、300、600、900 mg/L) were determined,which were assessed using subordinate function methods under the stress of 600 mg/L Cu~(2+).The results showed that the germination energy,germination rate,germination index,vigor index,root length,shoot length,fresh weight,number of lateral root were all decreased with the increasing of Cu~(2+) stress.According to average subordinate function,different hemp Cu-tolerance were classified:Y1、Q1、BM were low Cu-tolerance cultivars,J1、Y5、W1 were middle Cu-tolerance cultivars,Y6、YW6、Y7 were high Cu-tolerance cultivars.
引文
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[19]王翰,杨小录,何九军,等.重金属铜Cu(Ⅱ)对萝b种子萌发及幼苗叶绿素合成的影响[J].天水师范学院学报,2010,30(5):36-38.
[20]陈伟,张苗苗,宋阳阳,等.重金属胁迫对4种草坪草种子萌发的影响[J].草地学报,2013,21(3):556-563.
[21]高扬,辛树权,何锦冰,等.铜对大蒜根尖细胞有丝分裂影响的研究[J].北方园艺,2008(6):12-14.
[22]努扎艾提·艾比布,刘云国,宋华晓,等.重金属Zn、Cu对香根草生理生化指标的影响及其积累特性研究[J].农业环境科学学报,2010,29(1):54-59.
[23]赵玉红,拉巴曲吉,罗布,等.铜、镉、铅、锌对4种豆科植物种子萌发的影响[J].种子,2017,36(1):22-28.
[24]杨锦忠.玉米的重金属胁迫及其抗性[J].玉米科学,2000,8(3):62-66.
[2]徐云.大麻钾营养及耐低钾胁迫研究[D].昆明:云南大学,2016.
[3]唐东民,伍钧,唐勇,等.重金属胁迫对植物的毒害及其抗性机理研究进展[J].四川环境,2008,27(5):79-83.
[4]李广云,曹永富,赵书民,等.土壤重金属危害及修复措施[J].山东林业科技,2011,41(6):96-101.
[5]崔斌,王凌,张国印,等.土壤重金属污染现状与危害及修复技术研究进展[J].安徽农业科学,2012,40(1):373-375.
[6]郭鸿彦,杨明,谢晓慧,等.云南工业大麻产业化发展前景广阔[J].中国麻业,2002,24(4):46-49.
[7]梁淑敏,许艳萍,陈裕,等.工业大麻对重金属污染土壤的治理研究进展[J].生态学报,2013,33(5):1347-1356.
[8]许艳萍,陈璇,郭孟璧,等.4种重金属胁迫对工业大麻种子萌发的影响[J].西部林业科学,2014(4):78-82.
[9]陈仲英,龙瑜菡,徐云,等.工业大麻品种萌发期及幼苗前期重金属Pb耐性评价研究[J].中国麻业科学,2016,38(3):97-104.
[10]朱李奎,陆维超,马开骠,等.温度、盐分、p H值和光照强度对毛白杨种子萌发的影响[J].种子,2017(4):87-90.
[11]Huang J,Zhang Y,Peng J S,et al.Fission Yeast HMT1 lowers seed cadmium through phytochelatin-dependent vacuolar sequestration in Arabidopsis[J].Plant Physiology,2012(158):1779-1788.
[12]周璐璐,伏兵哲,许冬梅,等.盐胁迫对沙芦草萌发特性影响及耐盐性评价[J].草业科学,2015,32(8):1252-1259.
[13]任安芝,高玉葆.铅、镉、铬单一和复合胁迫对青菜种子萌发的生物学效应[J].生态学杂志,2000,19(1):19-22.
[14]陈洁,金晓玲,宁阳,等.3种含笑属植物抗寒生理指标的筛选及评价[J].河南农业科学,2016,45(2):113-118.
[15]杜玉玲,潘晨慧,李丹阳,等.利用隶属函数值法对7种一、二年生花卉耐盐性的综合评价[J].江苏农业科学,2017,45(6):105-111.
[16]张文娟,李悦,陈忠林,等.重金属胁迫对高羊茅和黑麦草种子萌发及幼苗生理特性的影响[J].安徽农业科学,2017,45(6):1-4.
[17]王慧芳,张凤杰,那日苏,等.铜胁迫对玉米幼苗生长的影响[J].安徽农业科学,2016(9):60-61.
[18]赵玉红,拉巴曲吉,罗布,等.铜、镉、铅、锌对4种豆科植物种子萌发的影响[J].种子,2017,36(1):22-28.
[19]王翰,杨小录,何九军,等.重金属铜Cu(Ⅱ)对萝b种子萌发及幼苗叶绿素合成的影响[J].天水师范学院学报,2010,30(5):36-38.
[20]陈伟,张苗苗,宋阳阳,等.重金属胁迫对4种草坪草种子萌发的影响[J].草地学报,2013,21(3):556-563.
[21]高扬,辛树权,何锦冰,等.铜对大蒜根尖细胞有丝分裂影响的研究[J].北方园艺,2008(6):12-14.
[22]努扎艾提·艾比布,刘云国,宋华晓,等.重金属Zn、Cu对香根草生理生化指标的影响及其积累特性研究[J].农业环境科学学报,2010,29(1):54-59.
[23]赵玉红,拉巴曲吉,罗布,等.铜、镉、铅、锌对4种豆科植物种子萌发的影响[J].种子,2017,36(1):22-28.
[24]杨锦忠.玉米的重金属胁迫及其抗性[J].玉米科学,2000,8(3):62-66.