产脂肪酶芽孢杆菌对肉鸡生长性能、养分表观利用率及小肠脂肪酶活性的影响
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摘要
为了研究产脂肪酶芽孢杆菌EA-30-A对肉鸡生长性能、养分表观利用率及小肠脂肪酶活性的影响,试验选用1日龄健康肉鸡240只,随机分为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组,每组3个重复,每个重复20只。对照组饲喂基础日粮,试验Ⅰ组、试验Ⅱ组和试验Ⅲ组分别饲喂添加0.5%、1.0%和2.0%EA-30-A菌剂的试验日粮,试验期42 d。结果表明:在基础日粮中添加1.0%产脂肪酶芽孢杆菌EA-30-A能显著降低料重比(P<0.05);添加不同水平的EA-30-A均有提高肉鸡干物质、总能和粗脂肪表观利用率的趋势,其中添加1.0%水平时达到显著水平(P<0.05);添加不同量的EA-30-A均可不同程度地提高21和42日龄肉鸡小肠内脂肪酶活力,但对前期影响显著(P<0.05)。
The experiment was conducted to investigate the effects of dietary supplementation of Bacillus named EA-30-A producing lipase on performance,nutrients apparent utilization and intestinal lipase activity of broilers. A total of 240 one-day old healthy AA broilers were randomly allocated to 4 groups with 3 replicates per group and 20 broilers per replicate for 42 days. In the control group and the 3 experimental groups,0%,0. 5%,1. 0% and 2. 0% dietary agent were added into the basal diet respectively. The results showed that the experimental group adding 1. 0% inocula of EA-30-A significantly decreased the feed/gain ratio( P < 0. 05). Different levels of EA-30-A had a increasing trend of improving dry matter,gross energy and the apparent utilization of crude fat and the gourp adding 1. 0% dose reached the significant level( P < 0. 05). In addition,the intestinal lipase activity of AA broilers at the age of 21 days and 42 days were increased by different amount of agent EA-30-A at different degree,whereas the activity in the earlier stage was increased dramatically( P < 0. 05).
The experiment was conducted to investigate the effects of dietary supplementation of Bacillus named EA-30-A producing lipase on performance,nutrients apparent utilization and intestinal lipase activity of broilers. A total of 240 one-day old healthy AA broilers were randomly allocated to 4 groups with 3 replicates per group and 20 broilers per replicate for 42 days. In the control group and the 3 experimental groups,0%,0. 5%,1. 0% and 2. 0% dietary agent were added into the basal diet respectively. The results showed that the experimental group adding 1. 0% inocula of EA-30-A significantly decreased the feed/gain ratio( P < 0. 05). Different levels of EA-30-A had a increasing trend of improving dry matter,gross energy and the apparent utilization of crude fat and the gourp adding 1. 0% dose reached the significant level( P < 0. 05). In addition,the intestinal lipase activity of AA broilers at the age of 21 days and 42 days were increased by different amount of agent EA-30-A at different degree,whereas the activity in the earlier stage was increased dramatically( P < 0. 05).
引文
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[10]程志斌,吴淑云,张红兵,等.枯草芽孢杆菌对地面平养和笼养肉仔鸡生产性能及血液指标的影响[J].饲料工业,2012,33(18):38-42.
[11]樊月圆,廖国周,张红兵,等.枯草芽孢杆菌对地面平养和笼养肉鸡饲料营养利用率及血液生化指标的影响[J].饲料博览,2012(10):1-6.
[12]HABERER P,TOIT M,DICKS L M,et al.Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat,high-cholesterol diet-a preliminary in vivo trial[J].Int J Food Microbiol,2003,87(3):287-291.
[13]何前,陈庄,容庭,等.脂肪酶对黄羽肉鸡生产性能及养分表观利用率的影响[J].中国饲料,2010(19):17-19.
[2]刘桂武,周俊华,时本利,等.微生物脂肪酶在动物饲料中的应用研究[J].养猪,2011(5):11-12.
[3]郭晓军,郭威,袁洪水,等.一株饲用产脂肪酶芽孢杆菌的筛选及其紫外诱变育种[J].中国饲料,2015(12):27-29.
[4]岳增华,张贵义,陈山.植酸酶对肉鸡日粮养分表观利用率和表观代谢能的影响[J].饲料工业,2009,30(12):19-24.
[5]郭威,郭晓军,袁洪水,等.高效脂肪酶产生真菌的筛选、鉴定及产酶条件优化[J].饲料工业,2013,34(4):39-44.
[6]JIRAPHOCAKUL S,SULLIVAN T W,SHAHANI K M.Influence of a dried Bacillus subtilis culture and antibiotics on performance and intestinal microflora in turkeys[J].Poult Sci,1990,69(11):1966-1973.
[7]张仁义.地衣芽孢杆菌对肉鸡生长性能的影响及其作用机理的研究[D].福州:福建农林大学,2010.
[8]刘磊,朱立贤.芽孢乳杆菌对肉仔鸡生产性能、肠道发育和微生物菌群的影响[J].动物营养学报,2011,23(12),2136-2142.
[9]刘磊,刘影,成廷水,等.不同菌株芽孢杆菌对肉鸡生产性能的影响[J].饲料工业,2010,31(12):49-51.
[10]程志斌,吴淑云,张红兵,等.枯草芽孢杆菌对地面平养和笼养肉仔鸡生产性能及血液指标的影响[J].饲料工业,2012,33(18):38-42.
[11]樊月圆,廖国周,张红兵,等.枯草芽孢杆菌对地面平养和笼养肉鸡饲料营养利用率及血液生化指标的影响[J].饲料博览,2012(10):1-6.
[12]HABERER P,TOIT M,DICKS L M,et al.Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat,high-cholesterol diet-a preliminary in vivo trial[J].Int J Food Microbiol,2003,87(3):287-291.
[13]何前,陈庄,容庭,等.脂肪酶对黄羽肉鸡生产性能及养分表观利用率的影响[J].中国饲料,2010(19):17-19.