尼泊尔酸模不同部位提取物及5种主要成分抗炎作用研究
|
摘要
[目的]探索尼泊尔酸模(Rumex nepalensis Spreng.,RNS)抗炎的有效部位及活性成分。[方法]采用二甲苯致小鼠耳肿胀和角叉菜胶致大鼠足肿胀模型研究其不同部位和5种主要成分的抗炎作用,并测定大鼠足肿胀组织匀浆中肿瘤坏死因子-α(TNF-α)、大鼠白介素1β(IL-1β)、大鼠血清中超氧化物歧化酶(SOD)以及丙二醛(MDA)的含量。[结果]与模型组相比,30%乙醇部位(RNS2)、50%乙醇部位(RNS3)高剂量组对二甲苯致小鼠耳肿胀和角叉菜胶引起的大鼠足肿胀具有显著抑制作用(P<0.05),表明这2个部位是尼泊尔酸模的有效部位;大黄素、大黄酚和大黄素甲醚可显著降低角叉菜胶致大鼠足肿胀组织中的TNF-α、IL-1β以及血清中MDA的含量(P<0.05)。[结论]尼泊尔酸模具有显著的抗炎作用,其作用机制可能是与抑制脂质氧化终产物MDA,以及调控TNF-α和IL-1β等炎症因子的释放有关。
[Objective]The research aimed to study on anti-inflammatory activities of different fractions and five main ingredients from Rumex nepalensis.[Method]Different fractions and 5 main components were used to investigate the anti-inflammatory effects on auricle edema induced by dimethyl benzene in mice and hand paw swelling induced by carrageenan in rats,and the tumor necrosis factor-α(TNF-α)in rat foot swollen tissue homogenate, rat interleukin-1β(IL-1β), superoxide dismutase(SOD) and malondialdehyde(MDA) in rat serum were measured.[Result]Compared to the model group,the RNS2 and RNS3 could relieve swollen auricle of mice by dimethyl benzene and hand paw swelling ratsby carrageenan(P<0.05), indicating that these two parts were the main anti-inflammatory effective fractions. Meanwhile, emodin, chrysophanol and physcion showed anti-inflammatory effects on reducing the level of TNF-α, IL-1β and MDA(P<0.05). [Conclusion]RNS has significant anti-inflammatory effects. The mechanism may be related to the inhibition of MDA levels and the regulation of the release of inflammatory factors such as TNF-α and IL-1β.
[Objective]The research aimed to study on anti-inflammatory activities of different fractions and five main ingredients from Rumex nepalensis.[Method]Different fractions and 5 main components were used to investigate the anti-inflammatory effects on auricle edema induced by dimethyl benzene in mice and hand paw swelling induced by carrageenan in rats,and the tumor necrosis factor-α(TNF-α)in rat foot swollen tissue homogenate, rat interleukin-1β(IL-1β), superoxide dismutase(SOD) and malondialdehyde(MDA) in rat serum were measured.[Result]Compared to the model group,the RNS2 and RNS3 could relieve swollen auricle of mice by dimethyl benzene and hand paw swelling ratsby carrageenan(P<0.05), indicating that these two parts were the main anti-inflammatory effective fractions. Meanwhile, emodin, chrysophanol and physcion showed anti-inflammatory effects on reducing the level of TNF-α, IL-1β and MDA(P<0.05). [Conclusion]RNS has significant anti-inflammatory effects. The mechanism may be related to the inhibition of MDA levels and the regulation of the release of inflammatory factors such as TNF-α and IL-1β.
引文
[1] 中国科学院《中国植物志》编辑委员会.中国植物志:第25卷第1分册[M].北京:科学出版社,1998:160.
[2] 贵州省药品监督管理局.贵州省中药材、民族药材质量标准[M].贵阳: 贵州科技出版社,2003:24.
[3] KHETWAL K S,MANRAL K,PATHAK R P.Constituents of the aerial parts of Rumex nepalensis Spreng [J].Indian drugs,1987,24(7):328-329.
[4] 汪念,朱斌,绳慧峰,等.尼泊尔酸模的化学成分[J].中国实验方剂学杂志,2011,17(19):132-136.
[5] 邓丽娜,李博然,王国伟,等.尼泊尔酸模化学成分研究[J].中草药,2016,47(12):2095-2099.
[6] 李敬,张兰胜.尼泊尔酸模中蒽醌类成分研究[J].时珍国医国药,2016,27(2):298-300.
[7] 王洪玲,李波,钟国跃,等.尼泊尔酸模根的化学成分研究[J].中药材,2018,41(4):876-879.
[8] 魏春华,程虹毓,高燕萍,等.藏药矮紫堇解热镇痛抗炎作用的研究[J].中国新药杂志,2017,26(3):337-342.
[9] 王芳,王珍珍,茅向军,等.土大黄游离蒽醌类成分HPLC指纹图谱[J].中国实验方剂学杂志,2017,23 (8):72-76.
[10] 刘守信,张玮.大黄素对重症急性胰腺炎致全身炎症反应综合征大鼠腹腔巨噬细胞ICAM-3的体外作用[J].中国药物与临床,2012,12(8):1014-1016.
[11] 康培良,刘玮,冯浩,等.急性水肿型胰腺炎IL-1β和TNF-α的变化及大黄素的干预作用[J].肝胆胰外科杂志,2012,24(2):140-142.
[12] 李超,张丹参,赵晓倩,等.三种大黄酚制剂改善脑缺血/再灌注小鼠记忆功能的实验筛选研究[J].中国药理学通报,2010,26(12):1607-1612.
[13] 颜娟.大黄酚脂质体对小鼠脑缺血再灌注损伤的神经保护作用及机制研究[D].石家庄:河北医科大学,2014.
[14] 李晓红.大黄酸抗炎作用及机理研究[D].北京:中国协和医科大学,2010.
[15] 刘顺,李赫宇,赵玲.白藜芦醇降血尿酸、抗炎作用研究进展[J].药物评价与研究,2016,39(2):304-307.
[2] 贵州省药品监督管理局.贵州省中药材、民族药材质量标准[M].贵阳: 贵州科技出版社,2003:24.
[3] KHETWAL K S,MANRAL K,PATHAK R P.Constituents of the aerial parts of Rumex nepalensis Spreng [J].Indian drugs,1987,24(7):328-329.
[4] 汪念,朱斌,绳慧峰,等.尼泊尔酸模的化学成分[J].中国实验方剂学杂志,2011,17(19):132-136.
[5] 邓丽娜,李博然,王国伟,等.尼泊尔酸模化学成分研究[J].中草药,2016,47(12):2095-2099.
[6] 李敬,张兰胜.尼泊尔酸模中蒽醌类成分研究[J].时珍国医国药,2016,27(2):298-300.
[7] 王洪玲,李波,钟国跃,等.尼泊尔酸模根的化学成分研究[J].中药材,2018,41(4):876-879.
[8] 魏春华,程虹毓,高燕萍,等.藏药矮紫堇解热镇痛抗炎作用的研究[J].中国新药杂志,2017,26(3):337-342.
[9] 王芳,王珍珍,茅向军,等.土大黄游离蒽醌类成分HPLC指纹图谱[J].中国实验方剂学杂志,2017,23 (8):72-76.
[10] 刘守信,张玮.大黄素对重症急性胰腺炎致全身炎症反应综合征大鼠腹腔巨噬细胞ICAM-3的体外作用[J].中国药物与临床,2012,12(8):1014-1016.
[11] 康培良,刘玮,冯浩,等.急性水肿型胰腺炎IL-1β和TNF-α的变化及大黄素的干预作用[J].肝胆胰外科杂志,2012,24(2):140-142.
[12] 李超,张丹参,赵晓倩,等.三种大黄酚制剂改善脑缺血/再灌注小鼠记忆功能的实验筛选研究[J].中国药理学通报,2010,26(12):1607-1612.
[13] 颜娟.大黄酚脂质体对小鼠脑缺血再灌注损伤的神经保护作用及机制研究[D].石家庄:河北医科大学,2014.
[14] 李晓红.大黄酸抗炎作用及机理研究[D].北京:中国协和医科大学,2010.
[15] 刘顺,李赫宇,赵玲.白藜芦醇降血尿酸、抗炎作用研究进展[J].药物评价与研究,2016,39(2):304-307.