EV71-VP1抗原在双歧杆菌中表达的免疫效果检测
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摘要
目的检测表达肠道病毒71型(enterovirus 71,EV71)VP1基因的重组双歧杆菌免疫后小鼠Ig G、Ig A表达变化,并对免疫的孕鼠产下的新生小鼠注射EV71病毒,以检测免疫保护作用可否从母代传给子代。方法 ELISA法检测免疫小鼠血清Ig G、Ig A抗体效价;用EV71毒株对免疫孕鼠所产下的新生小鼠攻毒。结果两种抗体效价都有明显升高(P<0.01);实验组受病毒攻击新生小鼠存活率明显高于对照组。结论口服表达VP1蛋白的双歧杆菌能够激活体内免疫系统产生特异性抗体,重组双歧杆菌可通过免疫孕鼠而使新生幼鼠获得保护性抗体。为研制EV71亚单位口服活疫苗奠定了研究基础。
Objective To constructe a bifidobacterium recombinant carrying the VP1 gene from enterovirus71 to create an oral vaccine to prevent EV71 infection. To detect the futher protective effect of the recombinant using ELISA in vovo. Methods We studied the efficacy of an orally administered vaccine candidate developed using recombinant baculovirus displaying VP1( Bac-VP1) in a murine model. Gastrointestinal delivery of Bac-VP1 significantly induced VP1-specific humoral( Ig G) and mucosal( Ig A) immune responses.Results Bifidobacterium recombinant with the VP1 gene from enterovirus71 was constructed. The virus-neutralizing titer of anti-EV71-VP1 significantly increased than the control group. Expression of Ig A and Ig G increased after oral treatment. A lethal challenge test indicated that the survival rate of bac-VP1 and VP1 group was significantly higher than the control group during 14 days. Conclusion Bac-VP1 could be a promising oral vaccine candidate against EV71 infections. Thus,Bac-VP1 may provide a needle free,painless approach for immunization against EV71,thereby increasing patient compliance and consequently increasing vaccination coverage.
Objective To constructe a bifidobacterium recombinant carrying the VP1 gene from enterovirus71 to create an oral vaccine to prevent EV71 infection. To detect the futher protective effect of the recombinant using ELISA in vovo. Methods We studied the efficacy of an orally administered vaccine candidate developed using recombinant baculovirus displaying VP1( Bac-VP1) in a murine model. Gastrointestinal delivery of Bac-VP1 significantly induced VP1-specific humoral( Ig G) and mucosal( Ig A) immune responses.Results Bifidobacterium recombinant with the VP1 gene from enterovirus71 was constructed. The virus-neutralizing titer of anti-EV71-VP1 significantly increased than the control group. Expression of Ig A and Ig G increased after oral treatment. A lethal challenge test indicated that the survival rate of bac-VP1 and VP1 group was significantly higher than the control group during 14 days. Conclusion Bac-VP1 could be a promising oral vaccine candidate against EV71 infections. Thus,Bac-VP1 may provide a needle free,painless approach for immunization against EV71,thereby increasing patient compliance and consequently increasing vaccination coverage.
引文
1 Jing Yuan,Xinyi Tang,Kai Yin,et al.GITRL as a genetic adjuvant enhances enterovirus 71 VP1 DNA vaccine immunogenicity[J].Immunol Rese,2015,62(1):81-88
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3 Zhang Zhiqin,Xiang Guoshi,Huang Xiaotian.Research progress of enterovirus 71 VP1[J].Chinese J Zoonoses,2015,31(4):377-379
4 Chung YC,Ho MS,Wu JC,et al.Immunization with virus-like particles of enterovirus 71 elicits potent immune responses and protects mice against lethal challenge[J].Vaccine,2008,26(15):1855-1862
5 Wu CN,Lin YC,Fann C,et al.Protection against lethal enterovirus71infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus.[J].Vaccine,2001,20(5-6):895-904
6 Zhijian Yu,Zhen Huang,Chongwen Shao,et al.Oral administration of interferon-α2b-transformed Bifidobacterium longum protects BALB/c mice against coxsackievirus B3-induced myocarditis[J].Virology Journal,2011,8(1):525
7 Zhijian Yu,Zhen Huang,Chongwen Sao,et al.Oral immunization of mice using Bifidobacterium longum expressing VP1 protein from enterovirus 71[J].Arch Virol,2013,158(5):1071-1077
8 李江,马永平,李世彬,等.重叠延伸PCR法合成EV71 VP1-LTB融合基因及其原核表达.[J].中国微生态学杂志,2011,23(5):385-389
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11 郭志英,易成,王树人,等.婴儿双歧杆菌/胞嘧啶脱氨酶肿瘤靶向性基因治疗系统的构建[J].中国肺癌杂志,.2004,7(2):95-98
12 Yi Cheng,Huang Ying,Guo Zhiying,et al.Construction of bifidobacterium Infantis/CD targeting gene therapy system.[J].Chinese-German Journal of Clinical Oncology,2005,4(4):244-247
13 Zhuhua Li,Peng Ye,Yanbiao Yang,et al.Construction of dual suicide gene therapy system p TRKH2/CD and p TRKH2/UPRT in Bifidobacterium infantis and its characterization[J].Chinese-German Journal of Clinical Oncology,2009,8(7):375-380
14 云雪霞,胡静,陈清.霍乱弧菌肠毒素B亚单位基因在大肠杆菌和双歧杆菌的表达[J].中国人兽共患病学报,2007,23(2):168-171
15 王国富,高峰,薛士鹏,吴利先.幽门螺杆菌融合基因vac A-hpa A的克隆及在大肠杆菌和双歧杆菌中的表达[J].中国现代医学杂志,2011,21(35):4388-4391
16 侯鑫,刘俊娥.大肠杆菌-长双歧杆菌穿梭载体的构建及PTEN在长双歧杆菌中的表达[J].微生物学报,2006,46(3):347-352
17 刘大伟,孙忠科,郭燕红,等.长双歧杆菌NCC2705高效转化系统的建立及GFP表达[J].生物技术通讯,2010,21(6):808-811
18 Liu Mei-chen,Jiang Qiang,Zhou Bi-ying,et al.Dynamic observation of immune responses induced in domestic pigs by immunization with different doses of a recombinant bifidobacterium vaccine against Taenia solium[J].J Pathogen Biol,2014,9(7):613-616
19 Tan J,Li W,Zhang L.Construction and identification of recombinant vaccine Bifidobacterium bifidum(p GEX-Sj32)of Schistosoma japonicum[J].Chinese J Endemiol,2015,34(1):37-40
2 Zhou SL,Ying XL,Han X,et al.Characterization of the enterovirus 71VP1 protein as a vaccine candidate[J].J Med Virol,2015,87(2):256-262
3 Zhang Zhiqin,Xiang Guoshi,Huang Xiaotian.Research progress of enterovirus 71 VP1[J].Chinese J Zoonoses,2015,31(4):377-379
4 Chung YC,Ho MS,Wu JC,et al.Immunization with virus-like particles of enterovirus 71 elicits potent immune responses and protects mice against lethal challenge[J].Vaccine,2008,26(15):1855-1862
5 Wu CN,Lin YC,Fann C,et al.Protection against lethal enterovirus71infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus.[J].Vaccine,2001,20(5-6):895-904
6 Zhijian Yu,Zhen Huang,Chongwen Shao,et al.Oral administration of interferon-α2b-transformed Bifidobacterium longum protects BALB/c mice against coxsackievirus B3-induced myocarditis[J].Virology Journal,2011,8(1):525
7 Zhijian Yu,Zhen Huang,Chongwen Sao,et al.Oral immunization of mice using Bifidobacterium longum expressing VP1 protein from enterovirus 71[J].Arch Virol,2013,158(5):1071-1077
8 李江,马永平,李世彬,等.重叠延伸PCR法合成EV71 VP1-LTB融合基因及其原核表达.[J].中国微生态学杂志,2011,23(5):385-389
9 罗耀玲,王勇,黄雪萍,等.大肠埃希菌热不稳定肠毒素B亚单位在婴儿双歧杆菌中的表达[J].中国微生态学杂志,2008,20(2):113-115
10 谢清.人血管内皮细胞生长因子受体s Flt-1在婴儿双歧杆菌中的表达和鉴定[J].现代临床医学,2010,36(5):330-331
11 郭志英,易成,王树人,等.婴儿双歧杆菌/胞嘧啶脱氨酶肿瘤靶向性基因治疗系统的构建[J].中国肺癌杂志,.2004,7(2):95-98
12 Yi Cheng,Huang Ying,Guo Zhiying,et al.Construction of bifidobacterium Infantis/CD targeting gene therapy system.[J].Chinese-German Journal of Clinical Oncology,2005,4(4):244-247
13 Zhuhua Li,Peng Ye,Yanbiao Yang,et al.Construction of dual suicide gene therapy system p TRKH2/CD and p TRKH2/UPRT in Bifidobacterium infantis and its characterization[J].Chinese-German Journal of Clinical Oncology,2009,8(7):375-380
14 云雪霞,胡静,陈清.霍乱弧菌肠毒素B亚单位基因在大肠杆菌和双歧杆菌的表达[J].中国人兽共患病学报,2007,23(2):168-171
15 王国富,高峰,薛士鹏,吴利先.幽门螺杆菌融合基因vac A-hpa A的克隆及在大肠杆菌和双歧杆菌中的表达[J].中国现代医学杂志,2011,21(35):4388-4391
16 侯鑫,刘俊娥.大肠杆菌-长双歧杆菌穿梭载体的构建及PTEN在长双歧杆菌中的表达[J].微生物学报,2006,46(3):347-352
17 刘大伟,孙忠科,郭燕红,等.长双歧杆菌NCC2705高效转化系统的建立及GFP表达[J].生物技术通讯,2010,21(6):808-811
18 Liu Mei-chen,Jiang Qiang,Zhou Bi-ying,et al.Dynamic observation of immune responses induced in domestic pigs by immunization with different doses of a recombinant bifidobacterium vaccine against Taenia solium[J].J Pathogen Biol,2014,9(7):613-616
19 Tan J,Li W,Zhang L.Construction and identification of recombinant vaccine Bifidobacterium bifidum(p GEX-Sj32)of Schistosoma japonicum[J].Chinese J Endemiol,2015,34(1):37-40