黑素瘤与色素痣的转录组学研究
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摘要
目的分析黑素瘤与色素痣的差异表达基因,从转录组水平揭示黑素瘤的发病机制。方法本研究中选用4例黑素瘤和3例色素痣患者组织样本,提取RNA后用Illumina测序平台进行高通量测序。分析得到差异表达基因后,对其进行相关的功能聚类及相互作用分析。结果与色素痣比较,黑素瘤中表达上调的基因有249个,表达下调的基因有109个;差异表达基因功能富集分析的结果显示,黑素瘤中表达上调的基因更多的与免疫系统及其调节有关。结论免疫相关基因的改变可能在黑素瘤的发展中起重要作用。
Objective To analyse the differentially expressed genes of melanoma and pigmented nevus,and to reveal the pathogenesis of melanoma from the transcriptome level.Methods Four cases of melanoma patients and three pigmented nevus patients were enrolled in this study.After RNA extraction from melanoma and pigmented nevus tissues,high-throughput sequencing was performed using Illumina sequencing platform.Functional clustering and interaction analysis of the differentially expressed genes were further carried out.Results There were 249 genes up-regulated and 109 genes down-regulated in melanoma.Functional enrichment analysis of the differentially expressed genes showed that the up-regulated genes in melanoma were more related to the immune system and its regulation.Conclusion The expression changes of immune related genes may play important roles in the progression of melanoma.
Objective To analyse the differentially expressed genes of melanoma and pigmented nevus,and to reveal the pathogenesis of melanoma from the transcriptome level.Methods Four cases of melanoma patients and three pigmented nevus patients were enrolled in this study.After RNA extraction from melanoma and pigmented nevus tissues,high-throughput sequencing was performed using Illumina sequencing platform.Functional clustering and interaction analysis of the differentially expressed genes were further carried out.Results There were 249 genes up-regulated and 109 genes down-regulated in melanoma.Functional enrichment analysis of the differentially expressed genes showed that the up-regulated genes in melanoma were more related to the immune system and its regulation.Conclusion The expression changes of immune related genes may play important roles in the progression of melanoma.
引文
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[2]Bobby Y,Reddy,David M,et al.Somatic driver mutations in melanoma[J].Cancer,2017,123(11):2104-2117
[3]Mckean MA,Amaria R N.Multidisciplinary treatment strategies in high-risk resectable melanoma:role of adjuvant and neoadjuvant therapy[J].Cancer Treatment Reviews,2018,70:144-153
[4]Rastrelli M,Tropea S,Rossi C R,et al.Melanoma:epidemiology,risk factors,pathogenesis,diagnosis and classification[J].Vivo,2014,28(12):1005-1011
[5]石国光.10例色素痣恶变临床分析[J].皮肤病与性病,2007,29(2):9-10
[6]Chiriboga L,Meehan S,Osman I,et al.Endothelin-1in the tumor microenvironment correlates with melanoma invasion[J].Melanoma Research,2016,26(3):236-244
[7]Moro N,Mauch C,Zigrino P.Metalloproteinases in melanoma[J].European Journal of Cell Biology,2014,93(1-2):23-29
[8]Hodis E,Watson IR,Kryukov GV,et al.A Landscape of Driver Mutations in Melanoma[J].Cell,2012,150(2):251-263
[9]Wellbrock C,Karasarides M,Marais R.The RAFproteins take centre stage[J].Nature Reviews Molecular Cell Biology,2004,5(11):875-885
[10]Ott P A,Hodi F S,Robert C.CTLA-4 and PD-1/PD-L1 Blockade:New Immunotherapeutic Modalities with Durable Clinical Benefit in Melanoma Patients[J].Clinical Cancer Research,2013,19(19):5300-5309
[11]Hamid O,Robert C,Daud A,et al.Safety and Tumor Responses with Lambrolizumab(Anti-PD-1)in Melanoma[J].New England Journal of Medicine,2013,369(2):134-144
[12]Borland M G,Yao P L,Kehres E M,et al.PPARβ/δand PPARγInhibit Melanoma Tumorigenicity by Modulating Inflammation and Apoptosis[J].Toxicological Sciences,2017,159(2):436-448
[13]Ohno F,Nakahara T,Kido-Nakahara M,et al.Periostin Links Skin Inflammation to Melanoma Progression in Humans and Mice[J].International journal of molecular sciences 2019,20(1):169
[14]Zhou J,Nagarkatti P,Zhong Y,et al.Characterization of T-Cell Memory Phenotype after In Vitro Expansion of Tumor-infiltrating Lymphocytes from Melanoma Patients[J].Anticancer Research,2011,31(12):4099-4109
[15]Greening D W,Gopal S K,Xu R,et al.Exosomes and their roles in immune regulation and cancer[J].Seminars in Cell&Developmental Biology,2015,40:72-81