青海汉、回族胎膜早破与MMP-3 rs35068180、-8 rs11225395基因多态性（英文）
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摘要
目的利用PCR-RFLP及测序法分析青海地区回、汉族胎膜早破孕妇MMP-3rs35068180、-8 rs11225395基因多态性特点及是否具有民族差异性。方法提取胎膜早破孕妇和对照组组织DNA,利用PCR-RFLP及琼脂糖凝胶电泳、测序法检测基因多态性,并行相关性分析。结果 MMP-3 rs35068180A/A、A/Del、Del/Del基因型频率在汉族对照组和胎膜早破组中分别是80.53%、17.70%、1.77%和100.00%、0.00%、0.00%,两组基因型频率分布具有显著差异性(P=0.000);在回族中分别是81.61%、16.09%、2.30%和100.00%、0.00%、0.00%,两组基因型频率呈现显著差异性(P=0.003)。MMP-8 rs11225395三种基因型A/A、A/G、G/G在汉族对照组和胎膜早破组中基因型频率分别为17.14%、31.43%、51.43%和14.16%、57.52%、28.32%,两组相比,基因型频率分布具有显著性差异(P=0.002);回族对照组和胎膜早破基因型频率分布分别为17.24%、32.76%、50.00%和14.94%、57.47%、27.59%,两组基因型频率相比具有显著性差异(P=0.009)。结论 MMP-3 rs35068180、-8 rs11225395基因多态性在青海汉族、回族胎膜早破者间无民族差异性。同时,青海汉族、回族对照组和胎膜早破组中MMP-3 rs35068180、-8 rs11225395基因频率分布差异无统计学意义。
Objective To analyze the polymorphism of matrix metalloprotease-3(MMP-3) rs35068180 and-8 rs11225395 gene in pregnant women with premature rupture of membranes in Han-and Hui-Chinese in Qinghai province by PCR-RFLP and sequencing. Methods Genome DNA was extracted from preterm premature rupture of membranes and control group.The polymorphism of the gene was analyzed by PCR-RFLP,agarose gel electrophoresis and sequencing. Results The frequency of MMP-3 rs35068180 A/A,A/Del,Del/Del genotype was 80. 53%,17.70%,1.77% and 100.00%,0.00%,0.00%,respectively,in the Han control and the premature rupture of membranes groups,the genotype frequency was significantly different between two groups(P = 0.000).Meanwhile the frequency of genotype was 81.61%,16.09%,2.30% and 100.00%,0.00%,0.00%,respectively,in the control and the premature rupture of membranes group in Hui Chinese,and the genotype frequency was obviously different between two groups(P = 0. 003). The frequency of MMP-8 rs11225395 A/A,A/G,G/G genotype was 17. 14%,31. 43%,51.43% and 14.16%,57. 52%,28. 32%,respectively,in the Han control and the premature rupture of membranes groups,the genotype frequency was significantly different between two groups(P = 0.002).Meanwhile the frequency of genotype was 17.24%,32.76%,50.00% and 14.94%,57.47%,27.59%,respectively,in the control and the premature rupture of membranes group in Hui Chinese,and the genotype frequency was obviously different between two groups(P = 0.009).Conclusion The polymorphisms of MMP-3 rs35068180 and-8 rs11225395 may be related to the premature rupture of membranes in Qinghai Han and Hui Chinese pregnant women. Meanwhile,the allele frequencies of MMP-3 rs35068180 and-8 rs11225395 were not specific between Han and Hui nationality.
Objective To analyze the polymorphism of matrix metalloprotease-3(MMP-3) rs35068180 and-8 rs11225395 gene in pregnant women with premature rupture of membranes in Han-and Hui-Chinese in Qinghai province by PCR-RFLP and sequencing. Methods Genome DNA was extracted from preterm premature rupture of membranes and control group.The polymorphism of the gene was analyzed by PCR-RFLP,agarose gel electrophoresis and sequencing. Results The frequency of MMP-3 rs35068180 A/A,A/Del,Del/Del genotype was 80. 53%,17.70%,1.77% and 100.00%,0.00%,0.00%,respectively,in the Han control and the premature rupture of membranes groups,the genotype frequency was significantly different between two groups(P = 0.000).Meanwhile the frequency of genotype was 81.61%,16.09%,2.30% and 100.00%,0.00%,0.00%,respectively,in the control and the premature rupture of membranes group in Hui Chinese,and the genotype frequency was obviously different between two groups(P = 0. 003). The frequency of MMP-8 rs11225395 A/A,A/G,G/G genotype was 17. 14%,31. 43%,51.43% and 14.16%,57. 52%,28. 32%,respectively,in the Han control and the premature rupture of membranes groups,the genotype frequency was significantly different between two groups(P = 0.002).Meanwhile the frequency of genotype was 17.24%,32.76%,50.00% and 14.94%,57.47%,27.59%,respectively,in the control and the premature rupture of membranes group in Hui Chinese,and the genotype frequency was obviously different between two groups(P = 0.009).Conclusion The polymorphisms of MMP-3 rs35068180 and-8 rs11225395 may be related to the premature rupture of membranes in Qinghai Han and Hui Chinese pregnant women. Meanwhile,the allele frequencies of MMP-3 rs35068180 and-8 rs11225395 were not specific between Han and Hui nationality.
引文
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[2]Escobar-Padilla B.,Gordillo-Lara L.D,Martinez-Puon H.Risk factors associated with preterm birth in a second level hospital[J].Rev Med Inst Mex Seguro Soc,2017,55(4):424-8.
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[8]Abenhaim H.A.,Alrowaily N.,Czuzoj-Shulman N.,et al.Pregnancy outcomes in women with bariatric surgery as compared with morbidly obese women[J].J Matern Fetal Neonatal Med,2016,29(22):3596-601.
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[11]Kurdoglu M.,Kolusari A.,Adali E.,et al.Does residual amniotic fluid after preterm premature rupture of membranes have an effect on perinatal outcomes?12 years experience of a tertiary care center[J].Arch Gynecol Obstet,2010,281(4):601-7.
[12]Fonseca F.L.,da Costa Aguiar Alves B.,Azzalis L.A.,et al.Matrix Metalloproteases as Biomarkers of Disease[J].Methods Mol Biol,2017,1579:299-311.
[13]An F.,Du J.,Cao Y.,et al.,MMP8 polymorphism is associated with susceptibility to osteonecrosis of the femoral head in a Chinese Han population[J].Oncotarget,2017,8(13):21561-6.
[14]Basaran R.,Senol M.,Ozkanli S.,et al.Correlation of matrix metalloproteinase(MMP)-1,-2,-3,and-9 expressions with demographic and radiological features in primary lumbar intervertebral disc disease[J].J Clin Neurosci,2017,41:46-49.
[15]Chen J.,Liu W.,Cao Y.,et al.MMP-3 and MMP-8single-nucleotide polymorphisms are related to alcohol-induced osteonecrosis of the femoral head in Chinese males[J].Oncotarget,2017,8(15):25177-88.
[16]de Oliveira H.C.,Tschoeke A.,da Cruz G.C.,et al.MMP-1 and MMP-8 expression in giant-cell fibroma and inflammatory fibrous hyperplasia[J].Pathol Res Pract,2016,212(12):1108-12.
[17]Markelova E.V.,Romanchuk A.L.,Prosekova E.V.,et al.Assessing the level of matrix metal proteinases 1,8,9,their tissue inhibitor,type I,in cases of odontogenic phlegmons[J].Bratisl Lek Listy,2017,118(1):51-5.
[18]Dong Z.,Bonfil R.D.,Chinni S.,et al.Matrix metalloproteinase activity and osteoclasts in experimental prostate cancer bone metastasis tissue[J].Am J Pathol,2005,166(4):1173-86.
[19]Mehats C.,Schmitz T.,Marcellin L.,et al.Biochemistry of fetal membranes rupture[J].Gynecol Obstet Fertil,2011,39(6):365-9.
[20]Meraz Cruz N.,Beltran Montoya J.,Estrada Gutierrez G.,et al.Identification of extracellular matrix metalloprotease 3 in the fetal membrane of the rat and its possible implication in the rupture of chorioamniotic membranes[J].Ginecol Obstet Mex,2006,74(12):671-7.
[21]Chaemsaithong P.,Romero R.,Docheva N.,et al.Comparison of rapid MMP-8 and interleukin-6 point-of-care tests to identify intra-amniotic inflammation/infection and impending preterm delivery in patients with preterm labor and intact membranes[J].J Matern Fetal Neonatal Med,2017(37):1-17.
[22]Guo N.,Xu Y.,Cao Z.Absinthin attenuates LPS-induced ALI through MIP-1alpha-mediated inflammatory cell infiltration[J].Exp Lung Res,2015,41(9):514-24.
[23]Quintero P.A.,Knolle M.D.,Cala L.F.,et al.Matrix metalloproteinase-8 inactivates macrophage inflammatory protein-1 alpha to reduce acute lung inflammation and injury in mice[J].J Immunol,2010,184(3):1575-88.
[24]Rella J.M.,Jilma B.,Fabry A.,et al.MMP-8 genotypes influence the inflammatory response in human endotoxemia[J].Inflammation,2014,37(2):451-6.
[25]钟颖,周俊,周明.基质金属蛋白酶-9表达与早产胎膜早破的相关性研究[J].中国妇幼保健,2013,(9):1502-4.
[26]伍玲,侯红瑛,陈新娟,等.基质金属蛋白酶MMP-9在胎膜早破早产孕妇血清、羊水中的含量及意义[J].中国实用医药,2010,5(22):34-35.
[27]储赞军,岳天孚.巨噬细胞移动抑制因子及基质金属蛋白酶-9在胎膜早破产妇胎膜中的表达及意义[J].中国妇幼保健,2010,25(10):1442-6.
[2]Escobar-Padilla B.,Gordillo-Lara L.D,Martinez-Puon H.Risk factors associated with preterm birth in a second level hospital[J].Rev Med Inst Mex Seguro Soc,2017,55(4):424-8.
[3]Liu J.,Feng Z.C.,Wu J.The incidence rate of premature rupture of membranes and its influence on fetal-neonatal health:a report from mainland China[J].J Trop Pediatr,2010,56(1):36-42.
[4]Dars S.,Malik S.,Samreen I.,et al.Maternal morbidity and perinatal outcome in preterm premature rupture of membranes before 37 weeks gestation[J].Pak J Med Sci,2014,30(3):626-9.
[5]Herbst A,Kallen K.Time between membrane rupture and delivery and septicemia in term neonates[J].Obstet Gynecol,2007,110(3):612-8.
[6]Helmo F.R.,Alves E.A.R.Moreira R.A.A.,et al.,Intrauterine infection,immune system and premature birth[J].J Matern Fetal Neonatal Med,2017,(33):1-7.
[7]Hackney D.N.,Kuo K.,Petersen R.J.,et al.Determinants of the competing outcomes of intrauterine infection,abruption,or spontaneous preterm birth after preterm premature rupture of membranes[J].J Matern Fetal Neonatal Med,2016,29(2):258-63.
[8]Abenhaim H.A.,Alrowaily N.,Czuzoj-Shulman N.,et al.Pregnancy outcomes in women with bariatric surgery as compared with morbidly obese women[J].J Matern Fetal Neonatal Med,2016,29(22):3596-601.
[9]Mercer B.Antibiotics in the management of PROM and preterm labor[J].Obstet Gynecol Clin North Am,2012,39(1):65-76.
[10]Buhimschi C.S.,Bhandari V.,Hamar B.D.,et al.Proteomic profiling of the amniotic fluid to detect inflammation,infection,and neonatal sepsis[J].PLo S Med,2007,4(1):18-21.
[11]Kurdoglu M.,Kolusari A.,Adali E.,et al.Does residual amniotic fluid after preterm premature rupture of membranes have an effect on perinatal outcomes?12 years experience of a tertiary care center[J].Arch Gynecol Obstet,2010,281(4):601-7.
[12]Fonseca F.L.,da Costa Aguiar Alves B.,Azzalis L.A.,et al.Matrix Metalloproteases as Biomarkers of Disease[J].Methods Mol Biol,2017,1579:299-311.
[13]An F.,Du J.,Cao Y.,et al.,MMP8 polymorphism is associated with susceptibility to osteonecrosis of the femoral head in a Chinese Han population[J].Oncotarget,2017,8(13):21561-6.
[14]Basaran R.,Senol M.,Ozkanli S.,et al.Correlation of matrix metalloproteinase(MMP)-1,-2,-3,and-9 expressions with demographic and radiological features in primary lumbar intervertebral disc disease[J].J Clin Neurosci,2017,41:46-49.
[15]Chen J.,Liu W.,Cao Y.,et al.MMP-3 and MMP-8single-nucleotide polymorphisms are related to alcohol-induced osteonecrosis of the femoral head in Chinese males[J].Oncotarget,2017,8(15):25177-88.
[16]de Oliveira H.C.,Tschoeke A.,da Cruz G.C.,et al.MMP-1 and MMP-8 expression in giant-cell fibroma and inflammatory fibrous hyperplasia[J].Pathol Res Pract,2016,212(12):1108-12.
[17]Markelova E.V.,Romanchuk A.L.,Prosekova E.V.,et al.Assessing the level of matrix metal proteinases 1,8,9,their tissue inhibitor,type I,in cases of odontogenic phlegmons[J].Bratisl Lek Listy,2017,118(1):51-5.
[18]Dong Z.,Bonfil R.D.,Chinni S.,et al.Matrix metalloproteinase activity and osteoclasts in experimental prostate cancer bone metastasis tissue[J].Am J Pathol,2005,166(4):1173-86.
[19]Mehats C.,Schmitz T.,Marcellin L.,et al.Biochemistry of fetal membranes rupture[J].Gynecol Obstet Fertil,2011,39(6):365-9.
[20]Meraz Cruz N.,Beltran Montoya J.,Estrada Gutierrez G.,et al.Identification of extracellular matrix metalloprotease 3 in the fetal membrane of the rat and its possible implication in the rupture of chorioamniotic membranes[J].Ginecol Obstet Mex,2006,74(12):671-7.
[21]Chaemsaithong P.,Romero R.,Docheva N.,et al.Comparison of rapid MMP-8 and interleukin-6 point-of-care tests to identify intra-amniotic inflammation/infection and impending preterm delivery in patients with preterm labor and intact membranes[J].J Matern Fetal Neonatal Med,2017(37):1-17.
[22]Guo N.,Xu Y.,Cao Z.Absinthin attenuates LPS-induced ALI through MIP-1alpha-mediated inflammatory cell infiltration[J].Exp Lung Res,2015,41(9):514-24.
[23]Quintero P.A.,Knolle M.D.,Cala L.F.,et al.Matrix metalloproteinase-8 inactivates macrophage inflammatory protein-1 alpha to reduce acute lung inflammation and injury in mice[J].J Immunol,2010,184(3):1575-88.
[24]Rella J.M.,Jilma B.,Fabry A.,et al.MMP-8 genotypes influence the inflammatory response in human endotoxemia[J].Inflammation,2014,37(2):451-6.
[25]钟颖,周俊,周明.基质金属蛋白酶-9表达与早产胎膜早破的相关性研究[J].中国妇幼保健,2013,(9):1502-4.
[26]伍玲,侯红瑛,陈新娟,等.基质金属蛋白酶MMP-9在胎膜早破早产孕妇血清、羊水中的含量及意义[J].中国实用医药,2010,5(22):34-35.
[27]储赞军,岳天孚.巨噬细胞移动抑制因子及基质金属蛋白酶-9在胎膜早破产妇胎膜中的表达及意义[J].中国妇幼保健,2010,25(10):1442-6.