miR-15a-5p和miR-16-5p在骨肉瘤组织中的表达分析
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摘要
目的:使用microRNAs基因芯片及实时定量PCR法测定骨肉瘤组织中miR-15a-5p和miR-16-5p的相对表达含量,并与瘤旁组织对比,分析骨肉瘤细胞内miR-15a-5p和miR-16-5p的表达变化。方法:选取34例骨肉瘤组织蜡块样本,使用microRNAs基因芯片观察miR-15a-5p和miR-16-5p在骨肉瘤和瘤旁组织内的表达差异;实时定量PCR法测定骨肉瘤组织和瘤旁组织中miR-15a-5p和miR-16-5p的相对表达含量,并将两种结果对比分析。结果:microRNAs基因芯片结果显示,在骨肉瘤组织中,miR-15a-5p在肿瘤中的表达较瘤旁组织低1.79倍,miR-16-5p较瘤旁组织低1.62倍。实时定量PCR实验结果表明,miR-15a-5p和miR-16-5p表达较瘤旁组织降低,差异有统计学意义(P<0.05)。经过统计学计算,miR-15a-5p在肿瘤中的表达较瘤旁组织低3.14倍,miR-16-5p较瘤旁组织低5.65倍。结论:在骨肉瘤中,miR-15a-5p和miR-16-5p表达含量降低,提示这两种microRNAs在骨肉瘤中可能做为抑癌因子存在。
Objective: Use microRNA microarray chip and real time quantitative PCR method to analysis the relative expression levels of miR-15a-5p and miR-16-5p, then compare the difference between osteosarcoma paraffin block samples and peritumoral tissue.Methods: 34 cases of osteosarcoma paraffin block samples were chosen, microRNAs microarray chip and real time quantitative PCR method was used to measure the relative expression levels of miR-15a-5p and miR-16-5p. Results: microRNAs microarray chip results showed that the expression level of miR-15a-5p(miR-16-5p) in tumor tissue was 1.79(1.62) times lower than the adjacent tissue. Real time quantitative PCR results showed that compare with tumor adjacent tissues, the expression level of miR-15a-5p and miR-16-5p was low in osteosarcoma paraffin block samples. The results were considered significant through statistical analysis(P<0.05). Further analysis results showed that the expression level of miR-15a-5p(miR-16-5p) in tumor tissue was 3.14(5.65) times lower than the adjacent tissue.Conclusions: miR-15a-5p and miR-16-5p was low expressed in osteosarcoma cells, which suggest that these two microRNAs could be act as oncogene.
Objective: Use microRNA microarray chip and real time quantitative PCR method to analysis the relative expression levels of miR-15a-5p and miR-16-5p, then compare the difference between osteosarcoma paraffin block samples and peritumoral tissue.Methods: 34 cases of osteosarcoma paraffin block samples were chosen, microRNAs microarray chip and real time quantitative PCR method was used to measure the relative expression levels of miR-15a-5p and miR-16-5p. Results: microRNAs microarray chip results showed that the expression level of miR-15a-5p(miR-16-5p) in tumor tissue was 1.79(1.62) times lower than the adjacent tissue. Real time quantitative PCR results showed that compare with tumor adjacent tissues, the expression level of miR-15a-5p and miR-16-5p was low in osteosarcoma paraffin block samples. The results were considered significant through statistical analysis(P<0.05). Further analysis results showed that the expression level of miR-15a-5p(miR-16-5p) in tumor tissue was 3.14(5.65) times lower than the adjacent tissue.Conclusions: miR-15a-5p and miR-16-5p was low expressed in osteosarcoma cells, which suggest that these two microRNAs could be act as oncogene.
引文
[1]Jones KB,Salah Z,Del Mare S,et al.miRNA signatures associate with pathogenesis and progression of osteosarcoma[J].Cancer Res,2012,72(7):1865-1877
[2]Ottaviani G,Jaffe N.Clinical and pathologic study of two siblings with osteosarcoma[J].Med Pediatr Oncol,2002,38(1):62-64
[3]Geller DS,Gorlick R.Osteosarcoma:a review of diagnosis,management,and treatment strategies[J].Clin Adv Hematol Oncol,2010,8(10):705-718
[4]Bandi N,Zbinden S,Gugger M,et al.miR-15a and miR-16-1 are implicated in cell cycle regulation in a Rb-dependent manner and are frequently deleted or down-regulated in non-small cell lung cancer[J].Cancer Res,2009,69(13):5553-5559
[5]Xu F,Zhang X,Lei Y,et al.Loss of repression of Hu R translation by miR-16 may be responsible for the elevation of Hu R in human breast carcinoma[J].J Cell Biochem,2010,111(3):727-734
[6]Tsang WP,Kwok TT.Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells[J].J Nutr Biochem,2010,21(2):140-146
[7]Bottoni A,Piccin D,Tagliati F,et al.miR-15a and miR-16-1down-regulation in pituitary adenomas[J].J Cell Physiol,2005,204(1):280-285
[8]CK Cai,GY Zhao,LY Tian,et al.miR-15a and miR-16-1downregulate CCND1 and induce apoptosis and cell cycle arrest in osteosarcoma[J].Oncol Rep,2012,28(5):1764-1770
[9]Xu G,Wang J,Jia Y,et al.miR-142-3p functions as a potential tumor suppressor in human osteosarcoma by targeting HMGA1[J].Cell Physiol Biochem,2014,33(5):1329-1339
[10]Tang J,Zhao H,Cai H,et al.Diagnostic and prognostic potentials of microRNA-27a in osteosarcoma[J].Biomed Pharmacother,2015,71:222-226
[11]Yu LD,Jin RL,Gu PC,et al.Clinical significance of microRNA-130b in osteosarcoma and in cell growth and invasion[J].Asian Pac J Trop Med,2015,8(9):752-756
[12]Jones KB,Salah Z,Del Mare S,et al.miRNA signatures associate with pathogenesis and progression of osteosarcoma[J].Cancer Res,2012,72(7):1865-1877
[13]Poos K,Smida J,Nathrath M,et al.How microRNA and transcription factor co-regulatory networks affect osteosarcoma cell proliferation[J].PLo S Comput Biol,2013,9(8):e1003210
[14]Cao ZQ,Shen Z,Huang WY.Micro RNA-802 promotes osteosarcoma cell proliferation by targeting p27[J].Asian Pac J Cancer Prev,2013,14(12):7081-7084
[15]Osaki M,Takeshita F,Sugimoto Y,et al.Micro RNA-143 regulates human osteosarcoma metastasis by regulating matrix metalloprotease-13 expression[J].Mol Ther,2011,19(6):1123-1130
[16]Xu F.Loss of repression of Hu R translation by miR-16 may be responsible for the elevation of Hu R in human breast carcinoma[J].Journal of Cellular Biochemistry,2010,111(3):727-734
[17]Xia L.miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells[J].International Journal of Cancer,2008,123(2):372-379
[18]Bonci D.The miR-15a-miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities[J].Nature Medicine,2010,14(11):1271-1277
[19]Bhattacharya R.Nicoloso M.Arvizo R.Mi R-15a and Mi R-16 control Bmi-1 expression in ovarian cancer[J].Cancer Research,2009.69(23):9090-9095
[20]Oscier DG,Gardiner AG,Mould SJ,et al.Multivariate analysis of prognostic factors in CLL:clinical stage,IGVH gene mutational status,and loss or mutation of the p53 gene are independent prognostic factors[J].Blood,2002,100(4):1177-1184
[21]Calin GA,Dumitru CD,Shimizu M,et al.Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J].Proc Natl Acad Sci U S A,2002,99(24):15524-15529
[22]Calin GA,Ferracin M,Cimmino A,et al.A Micro RNA signature associated with prognosis and progression in chronic lymphocytic leukemia[J].N Engl J Med,2005,353(17):1793-1801
[23]Cimmino A,Calin GA,Fabbri M,et al.miR-15 and miR-16 induce apoptosis by targeting BCL2[J].Proc Natl Acad Sci U S A,2005,102(39):13944-13949
[24]Palamarchuk A,Efanov A,Nazaryan N,et al.13q14 deletions in CLL involve cooperating tumor suppressors[J].Blood,2010,115(19):3916-3922
[25]Klein U,Lia M,Crespo M,et al.The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia[J].Cancer Cell,2010,17(1):28-40
[2]Ottaviani G,Jaffe N.Clinical and pathologic study of two siblings with osteosarcoma[J].Med Pediatr Oncol,2002,38(1):62-64
[3]Geller DS,Gorlick R.Osteosarcoma:a review of diagnosis,management,and treatment strategies[J].Clin Adv Hematol Oncol,2010,8(10):705-718
[4]Bandi N,Zbinden S,Gugger M,et al.miR-15a and miR-16-1 are implicated in cell cycle regulation in a Rb-dependent manner and are frequently deleted or down-regulated in non-small cell lung cancer[J].Cancer Res,2009,69(13):5553-5559
[5]Xu F,Zhang X,Lei Y,et al.Loss of repression of Hu R translation by miR-16 may be responsible for the elevation of Hu R in human breast carcinoma[J].J Cell Biochem,2010,111(3):727-734
[6]Tsang WP,Kwok TT.Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells[J].J Nutr Biochem,2010,21(2):140-146
[7]Bottoni A,Piccin D,Tagliati F,et al.miR-15a and miR-16-1down-regulation in pituitary adenomas[J].J Cell Physiol,2005,204(1):280-285
[8]CK Cai,GY Zhao,LY Tian,et al.miR-15a and miR-16-1downregulate CCND1 and induce apoptosis and cell cycle arrest in osteosarcoma[J].Oncol Rep,2012,28(5):1764-1770
[9]Xu G,Wang J,Jia Y,et al.miR-142-3p functions as a potential tumor suppressor in human osteosarcoma by targeting HMGA1[J].Cell Physiol Biochem,2014,33(5):1329-1339
[10]Tang J,Zhao H,Cai H,et al.Diagnostic and prognostic potentials of microRNA-27a in osteosarcoma[J].Biomed Pharmacother,2015,71:222-226
[11]Yu LD,Jin RL,Gu PC,et al.Clinical significance of microRNA-130b in osteosarcoma and in cell growth and invasion[J].Asian Pac J Trop Med,2015,8(9):752-756
[12]Jones KB,Salah Z,Del Mare S,et al.miRNA signatures associate with pathogenesis and progression of osteosarcoma[J].Cancer Res,2012,72(7):1865-1877
[13]Poos K,Smida J,Nathrath M,et al.How microRNA and transcription factor co-regulatory networks affect osteosarcoma cell proliferation[J].PLo S Comput Biol,2013,9(8):e1003210
[14]Cao ZQ,Shen Z,Huang WY.Micro RNA-802 promotes osteosarcoma cell proliferation by targeting p27[J].Asian Pac J Cancer Prev,2013,14(12):7081-7084
[15]Osaki M,Takeshita F,Sugimoto Y,et al.Micro RNA-143 regulates human osteosarcoma metastasis by regulating matrix metalloprotease-13 expression[J].Mol Ther,2011,19(6):1123-1130
[16]Xu F.Loss of repression of Hu R translation by miR-16 may be responsible for the elevation of Hu R in human breast carcinoma[J].Journal of Cellular Biochemistry,2010,111(3):727-734
[17]Xia L.miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells[J].International Journal of Cancer,2008,123(2):372-379
[18]Bonci D.The miR-15a-miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities[J].Nature Medicine,2010,14(11):1271-1277
[19]Bhattacharya R.Nicoloso M.Arvizo R.Mi R-15a and Mi R-16 control Bmi-1 expression in ovarian cancer[J].Cancer Research,2009.69(23):9090-9095
[20]Oscier DG,Gardiner AG,Mould SJ,et al.Multivariate analysis of prognostic factors in CLL:clinical stage,IGVH gene mutational status,and loss or mutation of the p53 gene are independent prognostic factors[J].Blood,2002,100(4):1177-1184
[21]Calin GA,Dumitru CD,Shimizu M,et al.Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J].Proc Natl Acad Sci U S A,2002,99(24):15524-15529
[22]Calin GA,Ferracin M,Cimmino A,et al.A Micro RNA signature associated with prognosis and progression in chronic lymphocytic leukemia[J].N Engl J Med,2005,353(17):1793-1801
[23]Cimmino A,Calin GA,Fabbri M,et al.miR-15 and miR-16 induce apoptosis by targeting BCL2[J].Proc Natl Acad Sci U S A,2005,102(39):13944-13949
[24]Palamarchuk A,Efanov A,Nazaryan N,et al.13q14 deletions in CLL involve cooperating tumor suppressors[J].Blood,2010,115(19):3916-3922
[25]Klein U,Lia M,Crespo M,et al.The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia[J].Cancer Cell,2010,17(1):28-40