油用牡丹主要相关性状与SSR分子标记的关联分析
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摘要
利用筛选出的16对多态性简单重复序列(SSR)标记对36份产油量较好、观赏性较佳的牡丹材料进行多态性扫描,在分析其遗传多样性和群体结构的基础上,采用TASSEL软件的一般线性模型(GLM)进行标记与25个性状的关联分析。结果表明:16对多态性引物共检测到65条条带,每对引物所扩增出的条带变幅为2.00到8.00不等,平均每一个标记条带数为4.06;多态性信息含量(P_(I,C))为0.19～0.73,平均值为0.50;群体结构分析将供试材料划分为3个亚群,分别包含9、15和12份试验材料;通过关联分析,发现3个SSR位点与单株种子产量、聚合蓇葖果数等9个性状极显著关联(P<0.01)。研究表明:所选的36份牡丹种质资源群体的群体结构简单、具有较高的遗传多样性,适用于牡丹目标性状的关联分析。关联分析能够有效地找到与油用牡丹表型性状紧密连锁的SSR标记,用于分子标记辅助育种。
Sixteen simple sequence repeat(SSR) markers were screened for polymorphism among 36 accessions with good oil yield and good ornamental quality,by analyzing their genetic diversity and population structure.The TASSEL GLM(general linear model) programs was used to perform correlation analysis of the markers and 25 traits.The results showed that 16 of polymorphic primers detected a total of 65 bands,and the bands amplified by each pair varied from 2.00 to 8.00,with an average of 4.06 bands per marker.The mean polymorphism information content value was with a range from 0.19 to0.73,with an average of 0.50.Population structure analysis divided the test materials into 3 subgroups containing 9,15 and 12 materials,respectively.By association analysis,there were 3 SSR markers obviously associated with 9 horticultural traits including individual seed yield and number of aggregated fruits.The selected population of 36 peony germplasm resources has a simple population structure and high genetic diversity,which is suitable for the correlation analysis of peony target traits.Association analysis can effectively find SSR markers closely linked to phenotypic traits of oil peony for molecular marker assisted breeding.
Sixteen simple sequence repeat(SSR) markers were screened for polymorphism among 36 accessions with good oil yield and good ornamental quality,by analyzing their genetic diversity and population structure.The TASSEL GLM(general linear model) programs was used to perform correlation analysis of the markers and 25 traits.The results showed that 16 of polymorphic primers detected a total of 65 bands,and the bands amplified by each pair varied from 2.00 to 8.00,with an average of 4.06 bands per marker.The mean polymorphism information content value was with a range from 0.19 to0.73,with an average of 0.50.Population structure analysis divided the test materials into 3 subgroups containing 9,15 and 12 materials,respectively.By association analysis,there were 3 SSR markers obviously associated with 9 horticultural traits including individual seed yield and number of aggregated fruits.The selected population of 36 peony germplasm resources has a simple population structure and high genetic diversity,which is suitable for the correlation analysis of peony target traits.Association analysis can effectively find SSR markers closely linked to phenotypic traits of oil peony for molecular marker assisted breeding.
引文
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[14] 李健,胡永红.油用牡丹ISSR-PCR反应体系的建立和优化[J].分子植物育种,2017,15(8):3129-3135.
[15] 郭琪,郭大龙,郭丽丽,等.SSR分子标记在牡丹亲缘关系研究中的应用与研究进展[J].植物学报,2015,50(5):652-664.
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[17] 李莉莉.甘肃油用紫斑牡丹品种资源调查及评价[D].兰州:甘肃农业大学,2016.
[18] 王占营,王二强,郭亚珍,等.洛阳地区西北品种群油用型牡丹筛选初报[J].江西农业学报,2014,26(7):32-34.
[19] GONG L, DENG Z N. EST-SSR markers for gerbera (Gerbera hybrida)[J]. Molecular Breeding,2010,26(1):125-132.
[20] WU J, CAI C F, CHENG F Y, et al. Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences[J]. Molecular Breeding,2014,34(4):1853-1866.
[21] LI L, CHENG F Y, ZHANG Q X. Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae)[J]. American Journal of Botany,2011,98(2):16-18.
[22] YU J M, BUCKLE E S R. Genetic association mapping and genome organization of maize[J]. Current Opinion in Biotechnology,2006,17(2):155-160.
[23] 于海萍.牡丹SSR分子标记的开发及其在亲缘关系分析中的应用[D].北京:北京林业大学,2013.
[24] 郭丽丽,李明月,郭大龙,等.不同品种群牡丹表型SSR标记的遗传多样性及聚类分析[J].上海农业学报,2018,34(2):43-49.
[25] FRANKHAM R, BALLOU J D, BRISCOE D A. Introduction to conservation genetics[M]. Cambridge: Cambridge University Press,2002:29-62.
[26] 徐进,刘子梁,欧阳磊,等.柳杉初级种子园遗传多样性[J].东北林业大学学报,2014,42(4):1-5.
[27] 吴静,成仿云,庞利铮,等.紫斑牡丹表型性状与SSR分子标记的关联分析[J].北京林业大学学报,2016,38(8):80-87.
[2] YU H P, CHENG F Y, ZHONG Y, et al. Development of simple sequence repeat (SSR) markers from Paeonia ostii to study the genetic relationships among tree peonies (Paeoniaceae)[J]. Scientia Horticulturae,2013,164(17):58-64.
[3] WU J, CHENG F Y, CAI C F, et al. Association mapping for floral traits in cultivated Paeonia rockii based on SSR markers[J]. Molecular Genetics and Genomics,2017,292(1):187-200.
[4] 吴静,成仿云,钟原.紫斑牡丹花色表型数量分类研究[J].园艺学报,2016,43(5):947-956.
[5] 杨琴,袁涛,孙湘滨.两个牡丹品种开花过程中花色变化的研究[J].园艺学报,2015,42(5):930-938.
[6] 张雪莲,解志军,朱长红,等.油用牡丹产业发展前景及策略分析[J].农村经济与科技,2015,26(12):67-68.
[7] 朱恒星,戴前莉,李辉乾,等.基于SWOT分析的重庆市油用牡丹产业发展研究[J].山西农业大学学报(自然科学版),2016,36(3):166-169.
[8] 张正周,张驰松,郑旗,等.油用牡丹籽油水酶法提取工艺的研究[J].农业与技术,2016,36(3):30-32,46.
[9] ZHANG W X, YIN D, HUANG D Z, et al. Altitudinal patterns illustrate the invasion mechanisms of alien plants in temperate mountain forests of northern China[J]. Forest Ecology and Management,2015,351(1):1-8.
[10] 姚欢欢.油用牡丹种子油提取及剩余物综合利用[D].哈尔滨:东北林业大学,2013.
[11] 杨勇,刘佳坤,曾秀丽,等.四川牡丹部分野生居群种子脂肪酸组分比较[J].园艺学报,2015,42(9):1807-1814.
[12] 宋宏伟,刘少华,沈植国,等.不同栽培条件下油用牡丹种子产量及含油率[J].经济林研究,2018,36(1):105-109.
[13] 侯娜,王纪辉,吴运辉,等.贵州不同引种地油用牡丹种子营养成分含量分析[J].西南林业大学学报(自然科学版),2017,37(6):36-40.
[14] 李健,胡永红.油用牡丹ISSR-PCR反应体系的建立和优化[J].分子植物育种,2017,15(8):3129-3135.
[15] 郭琪,郭大龙,郭丽丽,等.SSR分子标记在牡丹亲缘关系研究中的应用与研究进展[J].植物学报,2015,50(5):652-664.
[16] 罗建让,张延龙,郭丽萍,等.35个栽培牡丹品种油用特性的评价研究[J].中国粮油学报,2016,31(10):60-65.
[17] 李莉莉.甘肃油用紫斑牡丹品种资源调查及评价[D].兰州:甘肃农业大学,2016.
[18] 王占营,王二强,郭亚珍,等.洛阳地区西北品种群油用型牡丹筛选初报[J].江西农业学报,2014,26(7):32-34.
[19] GONG L, DENG Z N. EST-SSR markers for gerbera (Gerbera hybrida)[J]. Molecular Breeding,2010,26(1):125-132.
[20] WU J, CAI C F, CHENG F Y, et al. Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences[J]. Molecular Breeding,2014,34(4):1853-1866.
[21] LI L, CHENG F Y, ZHANG Q X. Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae)[J]. American Journal of Botany,2011,98(2):16-18.
[22] YU J M, BUCKLE E S R. Genetic association mapping and genome organization of maize[J]. Current Opinion in Biotechnology,2006,17(2):155-160.
[23] 于海萍.牡丹SSR分子标记的开发及其在亲缘关系分析中的应用[D].北京:北京林业大学,2013.
[24] 郭丽丽,李明月,郭大龙,等.不同品种群牡丹表型SSR标记的遗传多样性及聚类分析[J].上海农业学报,2018,34(2):43-49.
[25] FRANKHAM R, BALLOU J D, BRISCOE D A. Introduction to conservation genetics[M]. Cambridge: Cambridge University Press,2002:29-62.
[26] 徐进,刘子梁,欧阳磊,等.柳杉初级种子园遗传多样性[J].东北林业大学学报,2014,42(4):1-5.
[27] 吴静,成仿云,庞利铮,等.紫斑牡丹表型性状与SSR分子标记的关联分析[J].北京林业大学学报,2016,38(8):80-87.