宁波甬江水域夏冬季节溺死兔内脏硅藻16SrDNA的鉴定
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摘要
目的采用PCR法检测夏季7月和冬季12月宁波甬江水域溺死家兔内脏组织中硅藻16SrDNA,评估其在夏季7月和冬季12月溺死鉴定中的应用价值。方法于夏季7月和冬季12月分别选取30只大白兔,总计60只大白兔,随机分为溺死组(n=10)、死后入水组(n=10)、空气栓塞组(n=10)。各组分别提取心、肝、肺、肾组织,应用PCR技术检测上述兔内脏硅藻16SrDNA。结果与死后入水组比较,夏季7月兔溺死组心、肝、肺、肾组织中硅藻16SrDNA检出率明显增高(P<0.05);而冬季12月溺死组仅心、肺组织中检见硅藻16SrDNA,与死后入水组比较无明显差异(P>0.05);夏季7月溺死兔组心、肝、肺、肾组织中硅藻16SrDNA检出率明显高于冬季季12月溺死兔组(P<0.05);夏季7月和冬季12月空气栓塞组心、肝、肺、肾组织中硅藻16SrDNA均未检出。结论宁波甬江水域夏季溺死兔中硅藻16SrDNA检出率高,PCR技术可用于溺死诊断;而冬季硅藻16SrDNA检出率低,运用该技术诊断溺死应慎重。
Objective To evaluate the application value of identification on drown by detection 16SrDNA of the diatoms in rabbits' internal organs in summer month of July and winter month of December in Yong Jiang River of Ningbo. Methods 60 Rabbits were randomly and medially divided into three groups in summer and winter: drowning group, postmortem immersion group and using only lethal aeroembolism as control group. Specimen including heart, liver, lung and kidney from each rabbit were tested with diatom 16SrDNA PCR method. Results Compared with postmortem immersion group, detection rate of diatom 16SrDNA of heart, liver, lung, renal tissue in drowning group was significantly higher than that in summer month of July(P<0.05), In December, the 16SrDNA of the drowning group was detected in heart and lung tissues, There was no significant difference compared with postmortem immersion group(P>0.05) In summer month of July, detection rate of 16SrDNA of heart, liver, lung, renal tissues in drowning group was significantly higher than that in winter month of December(P<0.05). Diatom 16SrDNA of heart, liver, lung, kidney tissues in air embolism group were not detected In summer month of July and winter month of December. Conclusion With the higher detection rate of diatom 16SrDNA in drowning rabbit in summer, the diatom 16SrDNA PCR method can be used for the diagnosis of drowning in Yongjiang River of Ningbo; while in winter, it should be carefully apllied with the lower detection rate of diatom 16SrDNA.
Objective To evaluate the application value of identification on drown by detection 16SrDNA of the diatoms in rabbits' internal organs in summer month of July and winter month of December in Yong Jiang River of Ningbo. Methods 60 Rabbits were randomly and medially divided into three groups in summer and winter: drowning group, postmortem immersion group and using only lethal aeroembolism as control group. Specimen including heart, liver, lung and kidney from each rabbit were tested with diatom 16SrDNA PCR method. Results Compared with postmortem immersion group, detection rate of diatom 16SrDNA of heart, liver, lung, renal tissue in drowning group was significantly higher than that in summer month of July(P<0.05), In December, the 16SrDNA of the drowning group was detected in heart and lung tissues, There was no significant difference compared with postmortem immersion group(P>0.05) In summer month of July, detection rate of 16SrDNA of heart, liver, lung, renal tissues in drowning group was significantly higher than that in winter month of December(P<0.05). Diatom 16SrDNA of heart, liver, lung, kidney tissues in air embolism group were not detected In summer month of July and winter month of December. Conclusion With the higher detection rate of diatom 16SrDNA in drowning rabbit in summer, the diatom 16SrDNA PCR method can be used for the diagnosis of drowning in Yongjiang River of Ningbo; while in winter, it should be carefully apllied with the lower detection rate of diatom 16SrDNA.
引文
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[2]余政梁,刘超,胡孙林,等.PCR-DHPLC法检测硅藻SSU基因在溺死鉴定中的应用[J].中国法医学杂志,2013,28(6):457-460.
[3]邓建强,刘宝琴,龙仁,等.硅藻COX-Ⅰ区种属特异性PCR用于法医学溺死诊断[J].川北医学院学报,2015,30(1):2-5.
[4]李鹏,徐曲毅,陈玲,等.检测藻类16Sr DNA特异性片段在溺死诊断中的应用[J].南方医科大学学报,2015,35(8):1215-1218.
[5]宋贤英,张捷,白春节,等.甬江干流浮游植物的群落结构及其季节变化[J].应用于环境生物学报,2013,19(4):663-669.
[6]周玉倩.硅藻18S r RNA鉴别实验家兔水中尸体死因的研究[D].芜湖:皖南医学院,2013.
[7]Zhao J,Liu C,Hu S L,et al.Microwave digestion-vacuum f iltration-automated scanning electron microscopyas a sensitive method for forensic diatom test[J].Int J Legal Med,2013,127(2):459-463.
[8]蔡海光,应捷,倪卓晖,等.宁波市三江流域夏季硅藻分布[J].法医学杂志,2016,32(6):413-414,419.
[9]Delabarde T,Keyser C,Tracqui A,et al.The potential of forensicanalysis on human bones found in riverine environment[J].Forensic Sci lnt,2013,228(1/2/3):1-5.
[10]何康,姬树伟,张宇红,等.干性溺死死亡1例[J].中国法医学杂志,2014,29(6):605-606,632.