基质辅助激光解吸电离飞行时间质谱在病原微生物鉴定中的应用
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摘要
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)作为一种新兴的蛋白质组学检测技术,以其快速、准确、高通量、易操作等特点,已广泛应用于微生物鉴定领域,受到国内外的广泛关注。本文介绍了MALDI-TOF MS技术的原理,并对其在病原微生物鉴定、同源性分析分型、耐药性检测方面的应用进行综述。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) has been widely used in microbial identification,as a novel proteomics detection technology. It has attracted worldwide attention because of its fast,accurate,high throughput and easy operation. In this paper,the principle of MALDI-TOF MS technology was introduced,as well as the application in microbial identification,homology analysis and typing and drug-resistance.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) has been widely used in microbial identification,as a novel proteomics detection technology. It has attracted worldwide attention because of its fast,accurate,high throughput and easy operation. In this paper,the principle of MALDI-TOF MS technology was introduced,as well as the application in microbial identification,homology analysis and typing and drug-resistance.
引文
[1] Seng P,Drancourt M,Gouriet F,et al. Ongoing revolution in bacteriology:routine identification of bacteria by matrixassisted laser desorption ionization time-of-flight mass spectrometry[J].Clin Infect Dis,2009,49(4):543-551.
[2]张明新,朱敏,王玫,等.应用基质辅助激光解析电离飞行时间质谱鉴定常见细菌和酵母菌[J].中华检验医学杂志,2011,34(11):988-992.
[3]汤旭,肖迪,张慧芳,等.MALDI-TOF-MS鉴定布鲁氏菌方法建立和评价[J].中国人兽共患病学报,2016,32(9):772-778.
[4]李东菊,朱元祺,梁冰.MALDI-TOF MS用于肺炎克雷伯菌同源性分析的初步研究[J].中国微生态学杂志,2016,28(5):528-532.
[5]魏超,代晓航,郭灵安,等.MALDI-TOF MS与16S rDNA方法对肠杆菌科微生物鉴定比较分析[J].质谱学报,2018,39(2):209-215.
[6]张丽荣,黄辉涛,方艳梅,等.霍乱弧菌基质辅助激光解析电离飞行时间质谱技术谱库的构建[J].中国卫生检验杂志,2018,28(2):172-175.
[7]程颖慧,王颖,杨晓朱,等.基质辅助激光解吸/电离飞行时间质谱法鉴定检疫性病菌炭疽菌[J].广东农业科学,2018,45(7):100-104.
[8]李滨洲,陈飞,郭珍珍,等.基质辅助激光解吸电离飞行时间质谱技术分离鉴定生鲜猪肉中沙门氏菌/大肠杆菌类似菌[J].食品安全质量检测学报,2018,9(4):717-722.
[9]俞静,陈峰,李媛睿,等.应用MALDI-TOF MS技术快速鉴定布鲁菌[J].国际医学检验杂志,2018,39(12):1443-1447.
[10] Fenselau CP. MALDI MS and strategies for protein analysis[J]. Analytical Chemistry,1997,69(21):661-665.
[11]顾兵,李永军.基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用及价值[J].检验医学,2013,31(11):814-816.
[12] Delphine M,Laurent B,Ingrid W,et al. Comparison of the Microflex LT and VITEK MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of?ight mass spectrometry[J]. J Clin Microbiol,2012,50(4):1313-1325.
[13] Nagy E,Maier T,Urban E,et al. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry[J]. Clinical Microbiology and Infection,2009,15(8):796-802.
[14] Li Y,Gu B,Liu G,et al. MALDI-TOF MS versus VITEK2ANC card for identification of anaerobic bacteria[J]. J Thorac Dis,2014,6(5):517-523.
[15] Handal N,Bakken JS,Smith TH,et al. Anaerobic blood culture isolates in a Norwegian university hospital:identification by MALDI-TOF MS vs 16S rRNA sequencing and antimicrobial susceptibility profiles[J]. APMIS,2015,123(9):749-758.
[16] Chao QT,Lee TF,Teng SH,et al. Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts[J]. PLoS One,2014,9(10):e109376.
[17] Marklein G,Josten M,Klanke U,et al. Matrix-assisted laser desorption ionization time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates[J]. J Clin Microbiol,2009,47(9):2912-2917.
[18]叶丽艳,郭玲,马艳宁,等.基质辅助激光解析电离飞行时间质谱技术在快速鉴定真菌中的临床应用研究[J].中华医院感染学杂志,2015,25(15):3361-3363.
[19] Pierre T. Becker,Annelies de B,Delphine M,et al. Identifcation of flamentous fungi isolates by MALDI-TOF mass spectrometry:clinical evaluation of an extended reference spectra library[J]. Med Mycol,2014,52(8):826-834.
[20]贺靖冬,侯敏,李庆,等. MALDI-TOF MS在呼吸道感染丝状真菌鉴定中的应用[J].检验医学与临床,2016,13(z2):14-16.
[21] Ling HZ,Yuan ZJ,Shen JL,et al. Accuracy of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinical pathogenic fungi:a Meta-analysis[J]. J Clin Microbiol,2014,52(7):2573-2582.
[22] Hotchkiss RS,Moldawer LL,Opal SM,et al. Sepsis and septic shock[J]. Nat Rev Dis Primers,2016(2):16045.
[23]马坚,俞万钧,胡必杰,等.通过基质辅助激光解析电离飞行时间质谱系统直接快速鉴定阳性血培养[J].中华医院感染学杂志,2017,27(12):2676-2679.
[24]芦嘉.MALDI-TOF MS在短期培养后胸腹腔积液中细菌快速鉴定的应用[J].现代检验医学杂志,2018,33(2):74-81.
[25]王琳,李佳萍,陈功祥,等.MALDI-TOF MS在中段尿样本细菌直接检测中的应用[J].检验医学,2015,30(2):108-112.
[26]张景皓,方毅,张艳梅,等.MALDI-TOF MS结合短期培养法快速检测中段尿样本中的病原菌[J].检验医学,2017,32(4):326-330.
[27]郑光辉.利用MALDI-TOF MS技术对脑脊液培养阳性原液快速鉴定[C].第七届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编.宁波:2016:1.
[28] Manuel W,Holger R,Thomas M,et al. MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages[J]. Int J Med Microbiol,2011,301(1):64-68.
[29] Verroken A,Bauraing C,Deplano A,et al. Epidemiological investigation of a nosocomial outbreak of multidrugresistant Corynebacterium striatum at one Belgian university hospital[J].Clin Microbiol Infect,2014,20(1):44-50.
[30]李进,胡韦维,张峰领,等.多重耐药铜绿假单胞菌的两种同源性分析方法的比较[J].第三军医大学学报,2018,40(14):1258-1262.
[31]闫军,王娉,赵晓美,等.基于MALDI-TOF/TOF技术的单增李斯特菌四种血清型的快速鉴别[J].中国农业科技导报,2018,20(10):138-145.
[32]郑秋月,战晓微,肖珊珊,等.沙门氏菌MALDI-TOF MS分型方法建立[J].中国公共卫生,2014,30(10):1344-1347.
[33] Anna F. Lau,Honghui Wang,Rebecca A,et al. A rapid matrixassisted laser desorption ionization-time of flight mass spectrometry-based method for single-plasmid tracking in an outbreak of carbapenem-resistant Enterobacteriaceae[J]. J Clin Microbiol,2014,52(8):2804-2812.
[34] Irene B,Stefan Z. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours[J]. J Clin Microbiol,2011,49(9):3321-3324.
[35] Sparbier K,Schubert S,Kostrzewa M. MBTASTRA:A suitable tool for fast antibiotic susceptibility testing[J]. Methods,2016,104(15):48-54.
[2]张明新,朱敏,王玫,等.应用基质辅助激光解析电离飞行时间质谱鉴定常见细菌和酵母菌[J].中华检验医学杂志,2011,34(11):988-992.
[3]汤旭,肖迪,张慧芳,等.MALDI-TOF-MS鉴定布鲁氏菌方法建立和评价[J].中国人兽共患病学报,2016,32(9):772-778.
[4]李东菊,朱元祺,梁冰.MALDI-TOF MS用于肺炎克雷伯菌同源性分析的初步研究[J].中国微生态学杂志,2016,28(5):528-532.
[5]魏超,代晓航,郭灵安,等.MALDI-TOF MS与16S rDNA方法对肠杆菌科微生物鉴定比较分析[J].质谱学报,2018,39(2):209-215.
[6]张丽荣,黄辉涛,方艳梅,等.霍乱弧菌基质辅助激光解析电离飞行时间质谱技术谱库的构建[J].中国卫生检验杂志,2018,28(2):172-175.
[7]程颖慧,王颖,杨晓朱,等.基质辅助激光解吸/电离飞行时间质谱法鉴定检疫性病菌炭疽菌[J].广东农业科学,2018,45(7):100-104.
[8]李滨洲,陈飞,郭珍珍,等.基质辅助激光解吸电离飞行时间质谱技术分离鉴定生鲜猪肉中沙门氏菌/大肠杆菌类似菌[J].食品安全质量检测学报,2018,9(4):717-722.
[9]俞静,陈峰,李媛睿,等.应用MALDI-TOF MS技术快速鉴定布鲁菌[J].国际医学检验杂志,2018,39(12):1443-1447.
[10] Fenselau CP. MALDI MS and strategies for protein analysis[J]. Analytical Chemistry,1997,69(21):661-665.
[11]顾兵,李永军.基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用及价值[J].检验医学,2013,31(11):814-816.
[12] Delphine M,Laurent B,Ingrid W,et al. Comparison of the Microflex LT and VITEK MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of?ight mass spectrometry[J]. J Clin Microbiol,2012,50(4):1313-1325.
[13] Nagy E,Maier T,Urban E,et al. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry[J]. Clinical Microbiology and Infection,2009,15(8):796-802.
[14] Li Y,Gu B,Liu G,et al. MALDI-TOF MS versus VITEK2ANC card for identification of anaerobic bacteria[J]. J Thorac Dis,2014,6(5):517-523.
[15] Handal N,Bakken JS,Smith TH,et al. Anaerobic blood culture isolates in a Norwegian university hospital:identification by MALDI-TOF MS vs 16S rRNA sequencing and antimicrobial susceptibility profiles[J]. APMIS,2015,123(9):749-758.
[16] Chao QT,Lee TF,Teng SH,et al. Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts[J]. PLoS One,2014,9(10):e109376.
[17] Marklein G,Josten M,Klanke U,et al. Matrix-assisted laser desorption ionization time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates[J]. J Clin Microbiol,2009,47(9):2912-2917.
[18]叶丽艳,郭玲,马艳宁,等.基质辅助激光解析电离飞行时间质谱技术在快速鉴定真菌中的临床应用研究[J].中华医院感染学杂志,2015,25(15):3361-3363.
[19] Pierre T. Becker,Annelies de B,Delphine M,et al. Identifcation of flamentous fungi isolates by MALDI-TOF mass spectrometry:clinical evaluation of an extended reference spectra library[J]. Med Mycol,2014,52(8):826-834.
[20]贺靖冬,侯敏,李庆,等. MALDI-TOF MS在呼吸道感染丝状真菌鉴定中的应用[J].检验医学与临床,2016,13(z2):14-16.
[21] Ling HZ,Yuan ZJ,Shen JL,et al. Accuracy of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinical pathogenic fungi:a Meta-analysis[J]. J Clin Microbiol,2014,52(7):2573-2582.
[22] Hotchkiss RS,Moldawer LL,Opal SM,et al. Sepsis and septic shock[J]. Nat Rev Dis Primers,2016(2):16045.
[23]马坚,俞万钧,胡必杰,等.通过基质辅助激光解析电离飞行时间质谱系统直接快速鉴定阳性血培养[J].中华医院感染学杂志,2017,27(12):2676-2679.
[24]芦嘉.MALDI-TOF MS在短期培养后胸腹腔积液中细菌快速鉴定的应用[J].现代检验医学杂志,2018,33(2):74-81.
[25]王琳,李佳萍,陈功祥,等.MALDI-TOF MS在中段尿样本细菌直接检测中的应用[J].检验医学,2015,30(2):108-112.
[26]张景皓,方毅,张艳梅,等.MALDI-TOF MS结合短期培养法快速检测中段尿样本中的病原菌[J].检验医学,2017,32(4):326-330.
[27]郑光辉.利用MALDI-TOF MS技术对脑脊液培养阳性原液快速鉴定[C].第七届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编.宁波:2016:1.
[28] Manuel W,Holger R,Thomas M,et al. MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages[J]. Int J Med Microbiol,2011,301(1):64-68.
[29] Verroken A,Bauraing C,Deplano A,et al. Epidemiological investigation of a nosocomial outbreak of multidrugresistant Corynebacterium striatum at one Belgian university hospital[J].Clin Microbiol Infect,2014,20(1):44-50.
[30]李进,胡韦维,张峰领,等.多重耐药铜绿假单胞菌的两种同源性分析方法的比较[J].第三军医大学学报,2018,40(14):1258-1262.
[31]闫军,王娉,赵晓美,等.基于MALDI-TOF/TOF技术的单增李斯特菌四种血清型的快速鉴别[J].中国农业科技导报,2018,20(10):138-145.
[32]郑秋月,战晓微,肖珊珊,等.沙门氏菌MALDI-TOF MS分型方法建立[J].中国公共卫生,2014,30(10):1344-1347.
[33] Anna F. Lau,Honghui Wang,Rebecca A,et al. A rapid matrixassisted laser desorption ionization-time of flight mass spectrometry-based method for single-plasmid tracking in an outbreak of carbapenem-resistant Enterobacteriaceae[J]. J Clin Microbiol,2014,52(8):2804-2812.
[34] Irene B,Stefan Z. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours[J]. J Clin Microbiol,2011,49(9):3321-3324.
[35] Sparbier K,Schubert S,Kostrzewa M. MBTASTRA:A suitable tool for fast antibiotic susceptibility testing[J]. Methods,2016,104(15):48-54.