复盐胁迫对木薯幼苗光合特性及抗氧化酶活性的影响
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摘要
为研究复盐胁迫对木薯幼苗光合特性和抗氧化酶活性的影响,本研究以‘华南8号’和‘桂热911’为代表试材,采用盆栽法,设置0(CK)、0.05、0.1、0.15、0.2 mol/L 5个复盐处理浓度,对不同浓度复盐处理后的木薯幼苗叶绿素含量、光合参数和抗氧化酶活性进行测定分析。结果表明,复盐胁迫下,‘华南8号’和‘桂热911’幼苗叶片中叶绿素含量呈先上升后下降的趋势,浓度为0.15 mol/L时叶绿素含量最高,分别是2.52、2.35 mg/g;光合参数Pn、Tr、Gs、Ci、WUE及Ls总体呈先上升后下降的趋势,低浓度复盐胁迫下光合速率‘华南8号’>‘桂热911’,高浓度复盐胁迫下品种间差异不显著。相同浓度复盐胁迫下‘桂热911’的SOD、POD和CAT活性均高于‘华南8号’。综合NaCl-Na2SO4胁迫对‘华南8号’和‘桂热911’光合指标和抗氧化酶活性的影响,‘桂热911’对NaCl-Na2SO4胁迫的耐受性高于‘华南8号’。
The aim is to study the effects of mixed saline stress on photosynthetic characteristics and antioxidant enzymes of cassava seedling. We chose‘SC8'and‘GR911'as representative test materialsand used potted method. The seedlings were treated with mixed saline treatment at 0(CK), 0.05, 0.1, 0.15 and 0.2 mol/L. With different concentrations of mixed saline, chlorophyll contents, photosynthetic parametersand antioxidant enzymes activity of the cassava seedlings were determined and analyzed. The results showedthat the chlorophyll contents in the leaves of‘SC8'and‘GR911'seedling went up first and then droppedunder mixed saline stress, reached the highest when NaCl-Na2 SO4 concentration was 0.15 mol/L, which was2.52 and 2.35 mg/g, respectively. The photosynthetic parameters Pn, Tr, Gs, Ci, WUE and Ls increased firstand then decreased, and the photosynthetic rate was‘SC8'>'GR911'under low mixed saline stress, but thedifference between‘SC8'and‘GR911'was not significant under high mixed saline stress. The enzymeactivities of SOD, POD and CAT in‘GR911'were higher than those in‘SC8'under the same concentration ofmixed saline stress. Comprehensive consideration of the effects of NaCl-Na2 SO4 stress on photosyntheticindexes and antioxidant enzyme activities of‘SC8'and‘GR911', NaCl-Na2 SO4 tolerance of‘GR911'is higher than that of‘SC8'.
The aim is to study the effects of mixed saline stress on photosynthetic characteristics and antioxidant enzymes of cassava seedling. We chose‘SC8'and‘GR911'as representative test materialsand used potted method. The seedlings were treated with mixed saline treatment at 0(CK), 0.05, 0.1, 0.15 and 0.2 mol/L. With different concentrations of mixed saline, chlorophyll contents, photosynthetic parametersand antioxidant enzymes activity of the cassava seedlings were determined and analyzed. The results showedthat the chlorophyll contents in the leaves of‘SC8'and‘GR911'seedling went up first and then droppedunder mixed saline stress, reached the highest when NaCl-Na2 SO4 concentration was 0.15 mol/L, which was2.52 and 2.35 mg/g, respectively. The photosynthetic parameters Pn, Tr, Gs, Ci, WUE and Ls increased firstand then decreased, and the photosynthetic rate was‘SC8'>'GR911'under low mixed saline stress, but thedifference between‘SC8'and‘GR911'was not significant under high mixed saline stress. The enzymeactivities of SOD, POD and CAT in‘GR911'were higher than those in‘SC8'under the same concentration ofmixed saline stress. Comprehensive consideration of the effects of NaCl-Na2 SO4 stress on photosyntheticindexes and antioxidant enzyme activities of‘SC8'and‘GR911', NaCl-Na2 SO4 tolerance of‘GR911'is higher than that of‘SC8'.
引文
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[2] Apel K, Hirt H. Reactive oxygen species:metabolism,oxidative stress,and signal transduction[J]. Annual Review of Plant Biology,2004,55:373-399.
[3] Allen R D. Dissection of oxidative stress tolerance using transgenic plants[J]. Plant Physiology,1995,107:1049-1054.
[4]吴强盛,刘琴.毛桃对盐胁迫的光合、抗氧化和离子响应[J].江西农业大学学报,2010,32(2):303-307.
[5]王金平,王舒甜,岳健敏,等.香樟幼苗对NaCl胁迫的生理响应[J].中国水土保持科学,2016,14(5):83-89.
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[7]薛晶晶,朱文丽,陈松笔.木薯华南8号组培苗对盐胁迫的生理响应[J].广西植物,2016,36(12):1460-1467.
[8]安飞飞,简纯平,杨龙,等.木薯幼苗叶绿素含量及光合特性对盐胁迫的响应[J].江苏农业学报,2015,31(3):500-504.
[9]杨龙,周锴,安飞飞,等.不同浓度盐胁迫对蛋黄木薯幼根抗氧化酶表达水平的影响[J].西南农业学报,2015,25(3):1057-1061.
[10]张振文,李开绵,叶剑秋,等.木薯光合作用特性研究[J].云南大学学报自然科学版,2007,29(6):628-632.
[11]陈冠喜,李开绵,叶剑秋,等.6个木薯品种生长发育及产量性状的初步研究[J].热带农业科学,2002,29(6):26-29.
[12] Li K, Zhu W, Zeng K, et al. Proteome Characterization of cassava(Manihot esculenta Crantz)somatic embryos, plantlets and tuberous roots[J]. Proteome Science, 2010(8):10.
[13] Gu B, Yao Q Q, Li K M, et al. Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors[J]. Starch,2013,65:253-263.
[14]朱新广,张其德.NaCl对光合作用影响的研究进展[J].植物学通报,1999,16(4):332-338.
[15]王遵亲.中国盐渍土[M].北京:科学出版社,1993.
[16]李合生.植物生物化学实验原理与技术[M].北京:高等教育出版社,2000:164-165.
[17] Amalo K, Chen G X, Asade K. Separate assays specific for ascorbate peroxidase and guaiacal peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase implants[J]. Plant Cell Physiology, 1994,35:497-504.
[18] Aebi H. Catalase in vitro[J]. Methods in Enzymology, 1984,105:121-126.
[19] Cheeseman J M. Mechanism of salinity tolerance in plants[J]. Plant Physiology,1988,87:547-550.
[20]林世青,许春辉,张其德,等.叶绿素荧光动力学在植物抗性生理学、生态学和农业现代化中的应用[J].植物学通报,1992,9(1):1-16.
[21] Von Wettatein D, Gough S, Kanangara C G. Chloro-phyll biosynthesis[J]. The Plant Cell,1995,7(7):1039-1057.
[22]牟建梅,张国芹,刘凤军,等.白菜叶绿素含量的测定方法筛选[J].江苏农业科学,2014,42(9):289-290.
[23]吴永波,薛建辉.盐胁迫对3种白蜡树幼苗生长与光合作用的影响[J].南京林业大学学报:自然科学版,2002,26(3):19-22.
[24]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片类囊体膜脂组成和功能的影响[J].植物生理学报,1997,23(2):105-110.
[25]王仁雷,华春,刘友良.盐胁迫对水稻光合特性的影响[J].南京农业大学学报,2002,25(4):11-14.
[26]刘全吉,孙学成,胡承孝,等.砷对小麦生长和光合作用特性的影响[J].生态学报,2009,29(2):854-859.
[27] Brugnoli E, Bjorkman O. Growth of cotton under continuous salinity stress:influence on allocation patern,stomatal and nonstomatal components of photosynthesis and dissipation of excess light energy[J]. Planta,1992,187:335-345.
[28] Jacob J, Lawlor D W. Stomatal and mesophyll limitations of photosynthesis in photosynthesis in phosphate deficient sunflower,maize and wheat plants[J]. Journal of Experimental Botany,1991,241:1001-1011.
[29]马旭俊,朱大海.植物超氧化物歧化酶(SOD)的研究进展[J].遗传,2003,25(2):225-231.
[30] Fazeli F, Ghorbanli M, Niknam V. Effect of drought on biomass,protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars[J]. Biology Plantarum, 2007,51(1):98-103.
[31] Selote D S, Khanna C R. Drought acclimation confers oxidative stress tolerance by inducing coordinated antioxidant defense at cellular and subcellular level in leaves of wheat seedlings[J].Physiology Plantarum,2006,127:494-506.
[32] Smirnoff N. The role of active oxygen in the response of plants to water deficit and desiccation[J]. New Phytologist,1993,125:27-58.
[33] Bowler C, Montagu M V, Inzc D. Superoxide dismutase and stress tolerance[J]. Annual Review of Plant Physiology and Plant Molecular Biology,1992,43(1):83-116.
[34]赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418.
[2] Apel K, Hirt H. Reactive oxygen species:metabolism,oxidative stress,and signal transduction[J]. Annual Review of Plant Biology,2004,55:373-399.
[3] Allen R D. Dissection of oxidative stress tolerance using transgenic plants[J]. Plant Physiology,1995,107:1049-1054.
[4]吴强盛,刘琴.毛桃对盐胁迫的光合、抗氧化和离子响应[J].江西农业大学学报,2010,32(2):303-307.
[5]王金平,王舒甜,岳健敏,等.香樟幼苗对NaCl胁迫的生理响应[J].中国水土保持科学,2016,14(5):83-89.
[6]韩浩章,王晓立,张颖,等.盐胁迫对秋季香樟幼苗抗氧化酶系统和光合特性的影响[J].浙江农业学报,2014,26(5):1235-1239.
[7]薛晶晶,朱文丽,陈松笔.木薯华南8号组培苗对盐胁迫的生理响应[J].广西植物,2016,36(12):1460-1467.
[8]安飞飞,简纯平,杨龙,等.木薯幼苗叶绿素含量及光合特性对盐胁迫的响应[J].江苏农业学报,2015,31(3):500-504.
[9]杨龙,周锴,安飞飞,等.不同浓度盐胁迫对蛋黄木薯幼根抗氧化酶表达水平的影响[J].西南农业学报,2015,25(3):1057-1061.
[10]张振文,李开绵,叶剑秋,等.木薯光合作用特性研究[J].云南大学学报自然科学版,2007,29(6):628-632.
[11]陈冠喜,李开绵,叶剑秋,等.6个木薯品种生长发育及产量性状的初步研究[J].热带农业科学,2002,29(6):26-29.
[12] Li K, Zhu W, Zeng K, et al. Proteome Characterization of cassava(Manihot esculenta Crantz)somatic embryos, plantlets and tuberous roots[J]. Proteome Science, 2010(8):10.
[13] Gu B, Yao Q Q, Li K M, et al. Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors[J]. Starch,2013,65:253-263.
[14]朱新广,张其德.NaCl对光合作用影响的研究进展[J].植物学通报,1999,16(4):332-338.
[15]王遵亲.中国盐渍土[M].北京:科学出版社,1993.
[16]李合生.植物生物化学实验原理与技术[M].北京:高等教育出版社,2000:164-165.
[17] Amalo K, Chen G X, Asade K. Separate assays specific for ascorbate peroxidase and guaiacal peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase implants[J]. Plant Cell Physiology, 1994,35:497-504.
[18] Aebi H. Catalase in vitro[J]. Methods in Enzymology, 1984,105:121-126.
[19] Cheeseman J M. Mechanism of salinity tolerance in plants[J]. Plant Physiology,1988,87:547-550.
[20]林世青,许春辉,张其德,等.叶绿素荧光动力学在植物抗性生理学、生态学和农业现代化中的应用[J].植物学通报,1992,9(1):1-16.
[21] Von Wettatein D, Gough S, Kanangara C G. Chloro-phyll biosynthesis[J]. The Plant Cell,1995,7(7):1039-1057.
[22]牟建梅,张国芹,刘凤军,等.白菜叶绿素含量的测定方法筛选[J].江苏农业科学,2014,42(9):289-290.
[23]吴永波,薛建辉.盐胁迫对3种白蜡树幼苗生长与光合作用的影响[J].南京林业大学学报:自然科学版,2002,26(3):19-22.
[24]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片类囊体膜脂组成和功能的影响[J].植物生理学报,1997,23(2):105-110.
[25]王仁雷,华春,刘友良.盐胁迫对水稻光合特性的影响[J].南京农业大学学报,2002,25(4):11-14.
[26]刘全吉,孙学成,胡承孝,等.砷对小麦生长和光合作用特性的影响[J].生态学报,2009,29(2):854-859.
[27] Brugnoli E, Bjorkman O. Growth of cotton under continuous salinity stress:influence on allocation patern,stomatal and nonstomatal components of photosynthesis and dissipation of excess light energy[J]. Planta,1992,187:335-345.
[28] Jacob J, Lawlor D W. Stomatal and mesophyll limitations of photosynthesis in photosynthesis in phosphate deficient sunflower,maize and wheat plants[J]. Journal of Experimental Botany,1991,241:1001-1011.
[29]马旭俊,朱大海.植物超氧化物歧化酶(SOD)的研究进展[J].遗传,2003,25(2):225-231.
[30] Fazeli F, Ghorbanli M, Niknam V. Effect of drought on biomass,protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars[J]. Biology Plantarum, 2007,51(1):98-103.
[31] Selote D S, Khanna C R. Drought acclimation confers oxidative stress tolerance by inducing coordinated antioxidant defense at cellular and subcellular level in leaves of wheat seedlings[J].Physiology Plantarum,2006,127:494-506.
[32] Smirnoff N. The role of active oxygen in the response of plants to water deficit and desiccation[J]. New Phytologist,1993,125:27-58.
[33] Bowler C, Montagu M V, Inzc D. Superoxide dismutase and stress tolerance[J]. Annual Review of Plant Physiology and Plant Molecular Biology,1992,43(1):83-116.
[34]赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418.