固相萃取柱法分离sn-2位单甘酯的研究
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摘要
为探寻与气相色谱分析相配套的前处理方法,以高效测定油脂中sn-2位(β位)脂肪酸组成及含量。考察了胰脂酶添加量对甘油三酯酶解程度的影响,并采用Florisil固相萃取柱对酶解产物中sn-2位单甘酯的分离纯化条件进行优化。采用高温气相色谱法分析Florisil固相萃取柱对油脂酶解产物中sn-2位单甘酯的分离能力,结果表明经过Florisil固相萃取法分离得到的sn-2位单甘酯纯度能达到95%;气相色谱法分析常见的7种油脂的sn-2位脂肪酸组成,结果表明Florisil固相萃取法与传统薄层色谱法测得的sn-2位脂肪酸组成不存在显著性差异。
A pretreatment method matching with gas chromatographic was found to high-efficiently analyze sn-2 fatty acid composition in oil. The effect of dosage of pancreatic lipase on the enzymolysis degree of triglyceride was investigated and the conditions of separation and purification of sn-2 monoacylglycerol in enzymatic hydrolysis product were optimized using a Florisil solid phase extraction column. The ability of Florisil solid phase extraction column separating sn-2 monoacylglycerol was analyzed by high temperature gas chromatography method. The results showed that the purity of sn-2 monoacylglycerol separated by Florisil solid phase extraction column could reach 95%. The sn-2 fatty acids compositions of seven kinds of common oils were analyzed by gas chromatography method,and the results showed that there was no significant differences between the compositions of sn-2 fatty acids obtained by Florisil solid phase extraction method and traditional thin layer chromatography( TLC).
A pretreatment method matching with gas chromatographic was found to high-efficiently analyze sn-2 fatty acid composition in oil. The effect of dosage of pancreatic lipase on the enzymolysis degree of triglyceride was investigated and the conditions of separation and purification of sn-2 monoacylglycerol in enzymatic hydrolysis product were optimized using a Florisil solid phase extraction column. The ability of Florisil solid phase extraction column separating sn-2 monoacylglycerol was analyzed by high temperature gas chromatography method. The results showed that the purity of sn-2 monoacylglycerol separated by Florisil solid phase extraction column could reach 95%. The sn-2 fatty acids compositions of seven kinds of common oils were analyzed by gas chromatography method,and the results showed that there was no significant differences between the compositions of sn-2 fatty acids obtained by Florisil solid phase extraction method and traditional thin layer chromatography( TLC).
引文
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[12]LIN M,LONG M X,LI G L,et al.Analysis of peanut using near-infrared spectroscopy and gas chromatography-mass spectrometry:correlation of chemical components and volatile compounds[J].Int J Food Prop,2016(3):508-520.
[13]COLIN C,PATRICK H,PAUL B,et al.Quantitation of the main constituents of some authentic sesame seed oils of different origin[J].Agric Food Chem,2006,54(17):6266-6270.
[14]毛程鑫,李桂华,李普选,等.牡丹籽油的脂肪酸组成及理化特性分析[J].现代食品科技,2014(4):142-146.
[2]周文化,郑仕宏,蒋爱民.植物油脂脂肪酸组成及位置分布研究进展[J].粮食与油脂,2011(3):4-6.
[3]徐俊杰,黄建花,宋志国,等.脂肪酸碳链长度对甘三酯体外模拟消化的影响[J].中国油脂,2014,39(6):49-52.
[4]郭玉宝,裘爱泳.甘三酯分析新方法:RP-HPLC/APCIMS[J].粮食与油脂,2002(5):42-44.
[5]WEI C F,XIAO H G,GUAN J T,et al.Structure identification of triacylglycerols in the seed oil of Momordica charantia L.var.abbreviata ser[J].J Am Oil Chem Soc,2009,86(1):33-39.
[6]徐同成,王文亮,程安玮,等.甘油三酯中脂肪酸分布位置检测方法研究进展[J].粮油加工,2010(11):35-36.
[7]李存法,何金环.固相萃取技术及其应用[J].天中学刊,2005,20(5):13-16.
[8]孙海红,钱叶苗,宋相丽,等.固相萃取技术的应用与研究新进展[J].现代化工,2011,10(2):21-26.
[9]李兴峰,张志国.固相萃取分离甘油三酯水解产物中的单甘酯[J].食品科技,2013(7):60-66.
[10]吴琳,刘四磊,魏芳,等.Florisil固相萃取法联用气相色谱测定油脂中sn-2位脂肪酸[J].中国油料作物学报,2015,37(2):227-233.
[11]李桂华,毕艳兰.油脂油料检验与分析技术[M].北京:化学工业出版社,2006:107-109.
[12]LIN M,LONG M X,LI G L,et al.Analysis of peanut using near-infrared spectroscopy and gas chromatography-mass spectrometry:correlation of chemical components and volatile compounds[J].Int J Food Prop,2016(3):508-520.
[13]COLIN C,PATRICK H,PAUL B,et al.Quantitation of the main constituents of some authentic sesame seed oils of different origin[J].Agric Food Chem,2006,54(17):6266-6270.
[14]毛程鑫,李桂华,李普选,等.牡丹籽油的脂肪酸组成及理化特性分析[J].现代食品科技,2014(4):142-146.