14-3-3γ在子宫肌瘤中的表达及其临床意义
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摘要
目的研究不同月经周期子宫肌瘤中14-3-3γ蛋白的表达情况及其与雌、孕激素受体、子宫肌瘤临床病理参数之间的关系。方法收集行子宫全切或次切的子宫肌瘤患者65例,取子宫肌瘤和正常子宫肌层组织,标本均经病理确诊并根据子宫内膜确定月经周期。免疫组织化学方法观察14-3-3γ的定位情况。Western blot方法检测组织中14-3-3γ、ERα、ERβ、PR蛋白的表达情况,分析14-3-3γ与月经周期、患者年龄、子宫肌瘤大小、肌瘤位置、肌瘤类型及与ERα、ERβ、PR等表达的相关性。结果(1)子宫肌瘤中14-3-3γ表达水平在不同月经周期及不同患者年龄、肌瘤大小、肌瘤类型和肌瘤位置中均无统计学差异(均P>0.05)。(2)子宫肌瘤中14-3-3γ表达水平明显低于正常子宫肌层(P<0.05),尤其是增殖期和分泌期。年龄<48岁组、子宫肌瘤大小4~8c m组、多发性子宫肌瘤组和肌壁间子宫肌瘤组中,子宫肌瘤中14-3-3γ表达水平均低于正常子宫肌层组织(均P<0.05)。(3)子宫肌瘤中ERα、ERβ、PR蛋白表达水平较正常子宫肌层增高(均P<0.05),且子宫肌瘤中14-3-3γ表达水平与ERα呈负相关性(r=-0.305,P<0.05),而与ERβ、PR的表达无相关性(r=-0.157、-0.036,均P>0.05)。结论14-3-3γ在子宫肌瘤中的表达不受月经周期、患者年龄及子宫肌瘤大小、类型或位置而改变。14-3-3γ在子宫肌瘤中的表达低于正常子宫肌层组织,其在生育年龄妇女和4~8cm大小子宫肌瘤、多发肌瘤和肌壁间肌瘤中的作用更为重要,该作用与雌激素受体α相关。
Objective To investigate the expression of 14-3-3γ in uterine myoma and its clinicopathological significance.Methods Uterine myoma and normal myometrium samples were collected from 65 patients scheduled for hysterectomy between May 2012 and July 2013 in the Second Affiliated Hospital of Wenzhou Medical University.All the samples were definitely diagnosed by pathological results,and the phases of menstrual cycle were determined according to the endometrium.The distribution of 14-3-3γ was examined by immunohistochemical staining and the expression of 14-3-3γ,ERα,ERβ,PR proteins in tissue specemens was detected by Western blot.The correlation of 14-3-3γ expression with menstrual cycle,patient age,myoma size,myoma type,myoma location and ER α,ER β,PR expression was analyzed.Results There was no significant difference in expression of 14-3-3γ among proliferative phase,secretory phase and atrophic phase in uterine myoma(P >0.05),and the expression of 14-3-3γ was not correlated with the age of patients,the sizes,types and locations of myoma(P >0.05).The expression of 14-3-3γ in uterine myoma was significantly lower than that in matched normal myometrium,especially during proliferative and secretory phase(P<0.05).The expression levels of 14-3-3γ in myomas of patients aged <48 years old;and in myomas of 4~8cm in diameter,in multiple myomas and intramural myomas were significantly lower than those in normal myometrium(P<0.05).The expression of ER α,ERβ and PR proteins in uterine myoma was significantly higher than that in normal myometrium(P<0.05).The expression of 14-3-3γ in uterine myoma was negatively correlated with ER α(r=-0.305,P=0.026),but was not correlated with ERβ and PR expression.Conclusion The expression of 14-3-3γ protein in uterine myoma is not changed with menstrual cycle,age,myoma size,myoma type or myoma location.The expression of 14-3-3γ protein in uterine myoma is lower than that in normal myometrium,which may be associated with action of ER α.
Objective To investigate the expression of 14-3-3γ in uterine myoma and its clinicopathological significance.Methods Uterine myoma and normal myometrium samples were collected from 65 patients scheduled for hysterectomy between May 2012 and July 2013 in the Second Affiliated Hospital of Wenzhou Medical University.All the samples were definitely diagnosed by pathological results,and the phases of menstrual cycle were determined according to the endometrium.The distribution of 14-3-3γ was examined by immunohistochemical staining and the expression of 14-3-3γ,ERα,ERβ,PR proteins in tissue specemens was detected by Western blot.The correlation of 14-3-3γ expression with menstrual cycle,patient age,myoma size,myoma type,myoma location and ER α,ER β,PR expression was analyzed.Results There was no significant difference in expression of 14-3-3γ among proliferative phase,secretory phase and atrophic phase in uterine myoma(P >0.05),and the expression of 14-3-3γ was not correlated with the age of patients,the sizes,types and locations of myoma(P >0.05).The expression of 14-3-3γ in uterine myoma was significantly lower than that in matched normal myometrium,especially during proliferative and secretory phase(P<0.05).The expression levels of 14-3-3γ in myomas of patients aged <48 years old;and in myomas of 4~8cm in diameter,in multiple myomas and intramural myomas were significantly lower than those in normal myometrium(P<0.05).The expression of ER α,ERβ and PR proteins in uterine myoma was significantly higher than that in normal myometrium(P<0.05).The expression of 14-3-3γ in uterine myoma was negatively correlated with ER α(r=-0.305,P=0.026),but was not correlated with ERβ and PR expression.Conclusion The expression of 14-3-3γ protein in uterine myoma is not changed with menstrual cycle,age,myoma size,myoma type or myoma location.The expression of 14-3-3γ protein in uterine myoma is lower than that in normal myometrium,which may be associated with action of ER α.
引文
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[2]Li N,Wang H,Fan J,et al.Overexpression of 14-3-3 8 promotes tumor metastasis and indicates poor prognosis in breast carcinoma[J].Oncotarget,2014,5(1):249-257.
[3]Boon S S,Banks L.High-risk human papillomavirus E6 oncoproteins interact with 14-3-3in a PDZ binding motif-dependent manner[J].J Virol,2014,87(3):1586-1595.
[4]Wang L,Huang H,Liu D,et al.Evaluation of 14-3-3 protein family levels and associated receptor expression of estrogen and progesterone in Human Uterine Leiomyomas[J].Gynecol Endocrinol,2012,28(8):665-668.
[5]Lv J,Zhu X,Dong K,et al.Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics[J].Fertil Steril,2008,90(5):1892-1898.
[6]Kovacs K A,Lengyel F,Wilhelm F,et al.Involvement of FKHR(FOX01)transcription factor in human uterus leiomyoma growth[J].Fertil Steril,2010,94(4):1491-1535.
[7]Kim E H,Kim J Y,Lee Y H,et al.Comparison of estrogen receptor-α,progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and-resistant uterine fibroids[J].Obstet Gynecol Sci,2014,57(2):144-150.