归芪多糖对衰老细胞周期及p53、p16蛋白的影响
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摘要
目的:研究归芪多糖对衰老的WI-38细胞的细胞周期及p53、p16蛋白表达的影响。方法:采用MTT法,研究不同浓度归芪多糖对WI-38细胞活力的影响,筛选出归芪多糖最佳作用浓度;采用流式细胞术分析细胞周期;Western blot法分析归芪多糖对p53、p16蛋白表达的影响。结果:0.016/mg·m L归芪多糖可明显促进细胞增殖;与衰老组细胞相比,归芪多糖可明显促进衰老细胞进入分裂期(P<0.01),并可显著降低衰老模型组细胞p53和p16蛋白的表达。结论:归芪多糖可延缓WI-38细胞的衰老,其作用机制与调控细胞周期,抑制p53-p21和p16-pRb信号通路有关。
Objective: To investigate the effect of Guiqi polysaccharide( GQP) on the cell cycle and the protein expression of p53 and p16 in senescence cells. Methods: MTT assay was used to observe the effect of different concentrations of GQP on the proliferation of WI-38 cells and screen the best concentration of GQP to promote the growth of WI-38 cells. Cell cycle was analyzed by flow cytometry,and Western blot analysis was performed to evaluate the protein expression of p53 and p16 in senescence cells. Results: 0. 016 mg / m L GQP was the best concentration of promoting cell proliferation. Compared with H2O2 model group,GQP could promote the senescence cells from G1-phase to S-phase and reduce the protein expression of p53 and p16,and the difference was significant( P < 0. 01). Conclusion: GQP has an protective effect on senescence cells. The anti-aging effect of GQP may be involved in regulating the cell cycle and weakening two signal pathways( p53-p21 and p16-pRb).
Objective: To investigate the effect of Guiqi polysaccharide( GQP) on the cell cycle and the protein expression of p53 and p16 in senescence cells. Methods: MTT assay was used to observe the effect of different concentrations of GQP on the proliferation of WI-38 cells and screen the best concentration of GQP to promote the growth of WI-38 cells. Cell cycle was analyzed by flow cytometry,and Western blot analysis was performed to evaluate the protein expression of p53 and p16 in senescence cells. Results: 0. 016 mg / m L GQP was the best concentration of promoting cell proliferation. Compared with H2O2 model group,GQP could promote the senescence cells from G1-phase to S-phase and reduce the protein expression of p53 and p16,and the difference was significant( P < 0. 01). Conclusion: GQP has an protective effect on senescence cells. The anti-aging effect of GQP may be involved in regulating the cell cycle and weakening two signal pathways( p53-p21 and p16-pRb).
引文
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[2]付小明,花芳,胡卓伟.细胞衰老与衰老相关性疾病[J].生理科学进展,2012,43(5):376.
[3]Pu XY,Li Y,Zhou LY.Deferring senile effect of polysaccharides from Angelica and Astragalus on aging mice[J].Human Health and Biomedical Engineering(HHBE),2011:289.
[4]蒲秀瑛,李言,张伟杰,等.归芪多糖延缓衰老作用的研究[J].天然产物研究与开发,2012,24:1630.
[5]Pu XY,Yu S,Fan WB,et al.Antitumor activity of polysaccharides from angelica and astragalus in vitro[J].Journal of Chemical and Pharmaceutical Research,2013,5(11):701.
[6]谢健,童培建,单乐天,等.H2O2氧化应激诱导兔椎间盘髓核细胞衰老的研究[J].中国骨伤,2013,26(4):332.
[7]徐文达,高平,杨文君.p53信号通路与肿瘤关系的研究进展[J].宁夏医科大学学报,2012,34(9):971.
[8]闫海龙,龚勇珍.氧化应激及p16和p53/p21与细胞衰老关系的研究进展[J].医学综述,2011,17(5):682.
[9]Cristofalo VJ,Sharf BB.Cellular senescence and DNA synthesis.Thymidine incorporation as a measure of population age in human diploid cells[J].Exptl Cell Res,1973,76(2):419.
[10]赵亮,张宗玉,童坦君.生物体衰老与复制衰老-体内与体外研究[J].生理学进展,2000,31(3):205.
[11]胡兵,安红梅,史云峰,等.补肾填精复方对WI38细胞周期及相关基因表达的影响[J].成都中医药大学学报,2007,30(2):35.
[12]Ohtsubo M,Roberts JM.Cyclin-dependent regulation of G1in mammalian fibroblasts[J].Sci,1993,259:1908.
[13]Shen YY,Hughes JT,Charlesworth JA,et al.Adiponectin is present in the urine in its native conformation,and specifically reduces the secretion of MCP-1 by proximal tubular cells[J].Nephrology,2008,13(1):405.