外源赤霉素对GAR小麦光合特性影响的初探
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摘要
为了厘清赤霉素在敏感性(GAR)小麦响应干旱中的作用。通过盆栽称重控水试验研究了2种水分条件下矮秆基因Rht13、Rht8及其对照rht基因对小麦抗旱性的影响。结果显示,在干旱条件下外源赤霉素对小麦抗旱性的提高起到明显的负调控作用,并且其对Rht13基因型及Rht8基因型小麦的负影响程度远大于rht基因型小麦。研究说明,外源赤霉素条件下,Rht13和Rht8品系小麦剧烈响应外源赤霉素,导致叶片过分伸长,过长的叶片导致叶绿素含量降低,从而降低了叶片的净光合速率,并进一步限制了地上部生物量的累积。
To clarify the role of gibberellin in response to drought in sensitive(GAR)wheat,the effects of dwarf genes Rht13, Rht8 and their control rht genes on drought resistance of wheat under two water conditions were studied by pot weighing and water control experiments. The results showed that exogenous gibberellin played an obvious negative regulation on the improvement of drought resistance of wheat under drought conditions, and its negative effect on Rht13 and Rht8 wheat were much greater than that of rht wheat. It was found that under the condition of exogenous gibberellin, Rht13 and Rht8 strain severe responsed to exogenous gibberellin, causing excessive leaf elongation. Excessive length of leaves led to a decrease in chlorophyll content in leaves, the net photosynthetic rate of leaves was reduced, the accumulation of aboveground biomass was further restricted.
To clarify the role of gibberellin in response to drought in sensitive(GAR)wheat,the effects of dwarf genes Rht13, Rht8 and their control rht genes on drought resistance of wheat under two water conditions were studied by pot weighing and water control experiments. The results showed that exogenous gibberellin played an obvious negative regulation on the improvement of drought resistance of wheat under drought conditions, and its negative effect on Rht13 and Rht8 wheat were much greater than that of rht wheat. It was found that under the condition of exogenous gibberellin, Rht13 and Rht8 strain severe responsed to exogenous gibberellin, causing excessive leaf elongation. Excessive length of leaves led to a decrease in chlorophyll content in leaves, the net photosynthetic rate of leaves was reduced, the accumulation of aboveground biomass was further restricted.
引文
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[15]陈利青,张瑞杰,张伟,等.不同糜子品种光合特性及农艺性状比较[J].山西农业科学,2019,47(4):553-559.
[16]王颂宇,杨绣娟,董泽鹏,等.麦豆套作对小麦农艺性状和光合特性的影响[J].山西农业科学,2019,47(2):180-184.
[17]赵跃锋,陈昆,张清华.盐胁迫对茄子光合特性、叶绿素荧光及保护酶活性的影响[J].山西农业科学,2018,46(11):1797-1799,1906.
[18]SONIA P W,REGULA B,ABIEL R,et al.Gibberellin deficiency confers both lodging and drought tolerance in small cereals[J].Front Plant Sci.,2016,7:643.
[19]COLEBROOK E H,THOMAS S G,PHILLIPS A L,et al.The role of gibberellin signalling in plant responses to abiotic stress[J].Journal of Experimental Biology,2014,217:67-75.
[20]NIR I,MOSHELION M,WEISS D.The Arabidopsis GIB-BERELLIN METHYL TRANSFERASE 1 suppresses gibberellin activity,reduces whole-plant transpiration and promotes drought tolerance in transgenic tomato[J].Plant Cell and Environment,2014,37(1):113-123.
[2]石建斌,王宁,周红,等.陆地棉中赤霉素合成途径关键酶基因的时空表达变化[J].华北农学报,2018,33(4):9-16.
[3]李浩亮,史金钟,何登峰,等.外源赤霉素对烤烟叶片生长和品质的影响[J].河南农业科学,2006(4):51-54.
[4]田佳星.毛白杨响应赤霉素的转录调控与等位变异解析[D].北京:北京林业大学,2016.
[5]WANG C,YANG A,YIN H,et al.Influence of water stress on endogenous hormone contents and cell damage of maize seedlings[J].Journal of Integrative Plant Biology,2008,50(4):427-434.
[6]HAMAYUN M,KHAN S A,KHAN A L,et al.Exogenous gibberellic acid reprograms soybean to higher growth and salt stress tolerance[J].Journal of Agricultural and Food Chemistry,2010,58(12):7226-7232.
[7]SCHROEDER J I,KWAK J M,ALLEN G J.Guard cell abscisic acid signalling and engineering drought hardiness in plants[J].Nature,2001,410:327-330.
[8]IUCHI S,KOBAYASHI M,TAJI T,et al.Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase,a key enzyme in abscisic acid biosynthesis in Arabidopsis[J].Plant J.,2001,27(4):325-333.
[9]韩德复.松嫩盐碱化草地羊草生长及种群建成与内源激素关系的研究[D].长春:东北师范大学,2015.
[10]GASPERINI D,GREENLAND A,HEDDEN P,et al.Genetic and physiological analysis of Rht8 in bread wheat:an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids[J].J.Exp.Bot.,2012,63(12):4419-4436.
[11]COELHO M,COLEBROOK E H,LLOYD D P A,et al.The involvement of gibberellin signalling in the effect of soil resistance to root penetration on leaf elongation and tiller number in wheat[J].Plant and Soil,2013,371(1/2):81-94.
[12]ELLIS M H,REBETZKE G J,CHANDLER P,et al.The effect of different height reducing genes on the early growth of wheat[J].Functional Plant Biology,2004,31(6):583-589.
[13]FARRELLl A D,OUGHAM H J,TOMOS A D.The effect of gibberellic acid on the response of leaf extension to lowtemperature[J].Plant Cell and Environment,2006,29(7):1329-1337.
[14]KIMS K,SOHN E Y,JOO G J,et al.Influence of jasmonic acid on endogenous gibberellin and abscisic acid in salt-stressed chard plant[J].Journal of Environmental Biology,2009,30(3):333-338.
[15]陈利青,张瑞杰,张伟,等.不同糜子品种光合特性及农艺性状比较[J].山西农业科学,2019,47(4):553-559.
[16]王颂宇,杨绣娟,董泽鹏,等.麦豆套作对小麦农艺性状和光合特性的影响[J].山西农业科学,2019,47(2):180-184.
[17]赵跃锋,陈昆,张清华.盐胁迫对茄子光合特性、叶绿素荧光及保护酶活性的影响[J].山西农业科学,2018,46(11):1797-1799,1906.
[18]SONIA P W,REGULA B,ABIEL R,et al.Gibberellin deficiency confers both lodging and drought tolerance in small cereals[J].Front Plant Sci.,2016,7:643.
[19]COLEBROOK E H,THOMAS S G,PHILLIPS A L,et al.The role of gibberellin signalling in plant responses to abiotic stress[J].Journal of Experimental Biology,2014,217:67-75.
[20]NIR I,MOSHELION M,WEISS D.The Arabidopsis GIB-BERELLIN METHYL TRANSFERASE 1 suppresses gibberellin activity,reduces whole-plant transpiration and promotes drought tolerance in transgenic tomato[J].Plant Cell and Environment,2014,37(1):113-123.