Pim1蛋白在乳腺不同病变组织中的表达差异及临床特征
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摘要
目的:探讨Pim1蛋白在健康人群、乳腺炎和乳腺癌患者乳腺组织中的表达差异和临床特征。方法:对我院空白组(39例正常居民)、乳腺炎组(39例乳腺炎患者)、癌旁组织组(39例乳腺癌患者的癌旁组织)、腺癌组织组(39例乳腺癌患者的乳腺癌组织)4组对象进行研究,检测各组对象乳腺组织中Pim1蛋白表达水平的差异,分析乳腺癌患者临床病情对其Pim1蛋白表达水平的影响。结果:乳腺炎组、腺癌组织组、癌旁组织组患者Pim1蛋白表达与空白组比较,差异均有统计学意义(P<0.05);癌旁组织组Pim1蛋白表达与腺癌组织组比较,差异有统计学意义(P<0.05);Pim1蛋白表达与乳腺癌患者的淋巴结转移、TNM分期、ER表达有关(P<0.05),与患者的年龄、组织学分级、PR及Her-2无关;以乳腺癌患者Pim1蛋白表达评定为因变量,以淋巴结转移、TNM分期、ER为自变量。影响乳腺癌患者Pim1蛋白表达的主要因素为:ER表达(OR=4.759)、淋巴结转移(OR=3.127)、TNM分期(OR=2.664)。结论:Pim1蛋白与乳腺炎、乳腺癌的发生发展存在密切关系,该指标检测可一定程度上评估患者乳腺炎症现状和癌变风险,对于乳腺癌早期诊断具有一定参考价值。
Objective:To explore the expression differences and clinical features of Pim1 protein in breast tissue of healthy population,mastitis and breast cancer patients.Methods:Between September 2017 and September 2018,during the blank group(39 cases of normal people)in our hospital,mastitis group(39 patients with mastitis),tissue adjacent to carcinoma group(39 cases of patients with breast cancer tissue adjacent to carcinoma),adenocarcinoma tissue group(39 patients with breast cancer of breast tissue)four groups of objects,object detection of four groups of Pim1 protein expression in breast tissue level difference,analysis of patients with breast cancer clinical condition on its Pim1 protein expression level.Results:Compared with the blank group,there were statistically significant differences in the expression of Pim1 protein in the mastitis group,the adenocarcinoma group and the paracancerous tissue group(P<0.05).The difference in the expression of Pim1 protein was statistically significant between the paracancerous group and the adenocarcinoma group(P<0.05).Pim1 protein expression is associated with lymph node metastasis,TNM staging,and ER expression in breast cancer patients.Pim1 protein expression is independent of patient age,histological grade,PR and Her-2.Pim1 protein expression was evaluated as a dependent variable in breast cancer patients,with lymph node metastasis,TNM staging,and ER as independent variables.The main factors affecting the expression of Pim1 protein in breast cancer patients were:ER expression(OR=4.759),lymph node metastasis(OR=3.127),TNM staging(OR=2.664).Conclusions:Pim1 protein is closely related to the occurrence and development of mastitis and breast cancer.The detection of this indicator can assess the current situation of breast inflammation and the risk of cancer to a certain extent,which is of certain reference value for the early diagnosis of breast cancer.
Objective:To explore the expression differences and clinical features of Pim1 protein in breast tissue of healthy population,mastitis and breast cancer patients.Methods:Between September 2017 and September 2018,during the blank group(39 cases of normal people)in our hospital,mastitis group(39 patients with mastitis),tissue adjacent to carcinoma group(39 cases of patients with breast cancer tissue adjacent to carcinoma),adenocarcinoma tissue group(39 patients with breast cancer of breast tissue)four groups of objects,object detection of four groups of Pim1 protein expression in breast tissue level difference,analysis of patients with breast cancer clinical condition on its Pim1 protein expression level.Results:Compared with the blank group,there were statistically significant differences in the expression of Pim1 protein in the mastitis group,the adenocarcinoma group and the paracancerous tissue group(P<0.05).The difference in the expression of Pim1 protein was statistically significant between the paracancerous group and the adenocarcinoma group(P<0.05).Pim1 protein expression is associated with lymph node metastasis,TNM staging,and ER expression in breast cancer patients.Pim1 protein expression is independent of patient age,histological grade,PR and Her-2.Pim1 protein expression was evaluated as a dependent variable in breast cancer patients,with lymph node metastasis,TNM staging,and ER as independent variables.The main factors affecting the expression of Pim1 protein in breast cancer patients were:ER expression(OR=4.759),lymph node metastasis(OR=3.127),TNM staging(OR=2.664).Conclusions:Pim1 protein is closely related to the occurrence and development of mastitis and breast cancer.The detection of this indicator can assess the current situation of breast inflammation and the risk of cancer to a certain extent,which is of certain reference value for the early diagnosis of breast cancer.
引文
1楚爱景,程旭锋,赵慧朵,等.柴芍乳癖颗粒对乳腺增生模型大鼠血清激素水平和乳腺组织中VEGF和bFGF蛋白表达水平的影响及其意义[J].吉林大学学报(医学版),2018,5(5):1005-1009,1118-1119.
2 殷倩倩,马东慎,魏瑜,等.不同分子亚型乳腺癌细胞系中RUNX3蛋白表达及定位研究[J].中国病理生理杂志,2018,34(9):1603-1609.
3 韩晓翠,李百隆,于立辉,等.盐霉素通过下调miR-221抑制乳腺癌MCF-7细胞增殖的实验观察[J].临床与实验病理学杂志,2018,(9):962-967.
4 熊萱,张远,李一,等.乳腺导管癌差异表达基因的生物信息学分析[J].贵州医药,2018,42(9):1027-1030,1153.
5 李平,任斌,李洪利,等.MiR-150-5p通过靶向调控Rab1A抑制乳腺癌细胞MDA-231的上皮-间质转化[J].中国生物化学与分子生物学报,2018,34(9):997-1003.
6 王文然,郭瑞,张华.乳腺恶性肿瘤间质中α-SMA表达及与其临床病理特征的关系[J].实用癌症杂志,2018,33(9):1393-1395,1406.
7 El-Nassan HB,Naguib BH,Beshay EA.Synthesis of new pyridothienopyrimidinone and pyridothienotriazolopyrimidine derivatives as pim-1inhibitors[J].J Enzyme Inhib Med Chem,2018,33(1):58-66.
8 张茂娜,张弘,张雷,等.微小RNA-326靶向CARD10抑制乳腺癌细胞的增殖和侵袭[J].山西医科大学学报,2018,(9):1063-1067.
9 刘琼雯,李秋雨,孙松,等.HIF-2α和Twist2在乳腺癌中的表达及其与预后的关系[J].中国现代医学杂志,2018,28(26):45-50.
10 盛文文.凋亡抑制因子Livin和Survivin在乳腺癌中的表达及临床意义[J].泰山医学院学报,2018,39(9):986-988.
11 师贞宗,汪令成,李金友,等.IncRNA MT1JP抑制乳腺癌细胞增殖并促进乳腺癌凋亡研究[J].临床和实验医学杂志,2018,17(17):1843-1847.
12 黄蓉,夏朝霞,杨峂,等.乳腺癌组织中SKA2mRNA和蛋白的表达水平及其与临床病理特征的相关性[J].浙江医学,2018,40(17):1922-1925.
13 许亚梅.丙泊酚下调ERK信号通路对乳腺癌细胞增殖和转移能力的影响[J].解放军预防医学杂志,2018,36(8):1032-1035.
14 Chojnacki K,Wińska P,Wielechowska M,et al.Biological properties and structural study of new aminoalkyl derivatives of benzimidazole and benzotriazole,dual inhibitors of CK2and PIM1kinases[J].Bioorg Chem,2018,80:266-275.
15 陈琢,孔艺然.沉默UHRF1对乳腺癌细胞增殖和转移的影响[J].实用肿瘤学杂志,2017,31(6):512-518.
16 欧阳林旗,王胜峰,魏凤,等.15-PGDH诱导药物逆转人乳腺癌MCF-7/ADR细胞耐药作用及机制[J].中国药学杂志,2018,53(16):1352-1358.
17 高丽娜,张萌萌,李丹丹,等.乳腺导管癌组织CUL4A蛋白表达及其临床意义[J].青岛大学医学院学报,2017,53(5):543-545,550.
18 陈建丽,陈晓文,张永喜.ACTL6A蛋白在乳腺癌组织中的表达及其临床意义[J].广东医科大学学报,2017,35(6):625-627.
19 Zhu HH,Wang XT,Sun YH,et al.Pim1overexpression prevents apoptosis in cardiomyocytes after exposure to hypoxia and oxidative stress via upregulating cell autophagy[J].Cell Physiol Biochem,2018,49(6):2138-2150.
20 欧阳雨晴,胡涵,章国良.葡萄糖调节蛋白和细胞色素P450酶在肿瘤化疗耐药中的研究进展[J].中国临床药理学杂志,2017,33(24):2637-2641.
21 Aouidate A,Ghaleb A,Ghamali M,et al.3D QSAR studies,molecular docking and ADMET evaluation,using thiazolidine derivatives as template to obtain new inhibitors of PIM1 kinase[J].Comput Biol Chem,2018,74:201-211.
2 殷倩倩,马东慎,魏瑜,等.不同分子亚型乳腺癌细胞系中RUNX3蛋白表达及定位研究[J].中国病理生理杂志,2018,34(9):1603-1609.
3 韩晓翠,李百隆,于立辉,等.盐霉素通过下调miR-221抑制乳腺癌MCF-7细胞增殖的实验观察[J].临床与实验病理学杂志,2018,(9):962-967.
4 熊萱,张远,李一,等.乳腺导管癌差异表达基因的生物信息学分析[J].贵州医药,2018,42(9):1027-1030,1153.
5 李平,任斌,李洪利,等.MiR-150-5p通过靶向调控Rab1A抑制乳腺癌细胞MDA-231的上皮-间质转化[J].中国生物化学与分子生物学报,2018,34(9):997-1003.
6 王文然,郭瑞,张华.乳腺恶性肿瘤间质中α-SMA表达及与其临床病理特征的关系[J].实用癌症杂志,2018,33(9):1393-1395,1406.
7 El-Nassan HB,Naguib BH,Beshay EA.Synthesis of new pyridothienopyrimidinone and pyridothienotriazolopyrimidine derivatives as pim-1inhibitors[J].J Enzyme Inhib Med Chem,2018,33(1):58-66.
8 张茂娜,张弘,张雷,等.微小RNA-326靶向CARD10抑制乳腺癌细胞的增殖和侵袭[J].山西医科大学学报,2018,(9):1063-1067.
9 刘琼雯,李秋雨,孙松,等.HIF-2α和Twist2在乳腺癌中的表达及其与预后的关系[J].中国现代医学杂志,2018,28(26):45-50.
10 盛文文.凋亡抑制因子Livin和Survivin在乳腺癌中的表达及临床意义[J].泰山医学院学报,2018,39(9):986-988.
11 师贞宗,汪令成,李金友,等.IncRNA MT1JP抑制乳腺癌细胞增殖并促进乳腺癌凋亡研究[J].临床和实验医学杂志,2018,17(17):1843-1847.
12 黄蓉,夏朝霞,杨峂,等.乳腺癌组织中SKA2mRNA和蛋白的表达水平及其与临床病理特征的相关性[J].浙江医学,2018,40(17):1922-1925.
13 许亚梅.丙泊酚下调ERK信号通路对乳腺癌细胞增殖和转移能力的影响[J].解放军预防医学杂志,2018,36(8):1032-1035.
14 Chojnacki K,Wińska P,Wielechowska M,et al.Biological properties and structural study of new aminoalkyl derivatives of benzimidazole and benzotriazole,dual inhibitors of CK2and PIM1kinases[J].Bioorg Chem,2018,80:266-275.
15 陈琢,孔艺然.沉默UHRF1对乳腺癌细胞增殖和转移的影响[J].实用肿瘤学杂志,2017,31(6):512-518.
16 欧阳林旗,王胜峰,魏凤,等.15-PGDH诱导药物逆转人乳腺癌MCF-7/ADR细胞耐药作用及机制[J].中国药学杂志,2018,53(16):1352-1358.
17 高丽娜,张萌萌,李丹丹,等.乳腺导管癌组织CUL4A蛋白表达及其临床意义[J].青岛大学医学院学报,2017,53(5):543-545,550.
18 陈建丽,陈晓文,张永喜.ACTL6A蛋白在乳腺癌组织中的表达及其临床意义[J].广东医科大学学报,2017,35(6):625-627.
19 Zhu HH,Wang XT,Sun YH,et al.Pim1overexpression prevents apoptosis in cardiomyocytes after exposure to hypoxia and oxidative stress via upregulating cell autophagy[J].Cell Physiol Biochem,2018,49(6):2138-2150.
20 欧阳雨晴,胡涵,章国良.葡萄糖调节蛋白和细胞色素P450酶在肿瘤化疗耐药中的研究进展[J].中国临床药理学杂志,2017,33(24):2637-2641.
21 Aouidate A,Ghaleb A,Ghamali M,et al.3D QSAR studies,molecular docking and ADMET evaluation,using thiazolidine derivatives as template to obtain new inhibitors of PIM1 kinase[J].Comput Biol Chem,2018,74:201-211.